Archives of Razi Institute最新文献

{"title":"Molecular Analysis of <i>Enterococcus Faecalis</i> Isolates in a 4-year Period.","authors":"Sattari-Maraji Azin, Emaneini Mohammad, Jabalameli Fereshteh, Beigverdi Reza","doi":"10.32592/ARI.2023.78.6.1873","DOIUrl":"10.32592/ARI.2023.78.6.1873","url":null,"abstract":"<p><p>In the present research, we aimed to determine the characteristics of E. faecalis strains collected from an Iranian Children's Hospital for four years. Sixty-seven E. faecalis isolates with virulence genes detection, variable-number tandem repeat (VNTR), and multiple-locus variable-number tandem repeat analysis (MLVA) typing were investigated. A high frequency of virulence genes belonged to gelatinase (73%) and Enterococcus faecalis (62%). The MLVA of 67 E. faecalis isolates revealed 52 VNTR patterns and 38 MLVA types (MTs). Furthermore, genetic diversities with the majority of the MT1 as a major MT in different Wards of the Children's Hospital were found.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"78 6","pages":"1873-1877"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Identification and Phylogenetic Study Based on the Fusion Gene of Newcastle Disease Virus Isolated from Broiler Poultry Farms in Markazi Province, Iran.","authors":"V Vahidi, S Ebrahimi, N Akbari, P Jafari","doi":"10.32592/ARI.2023.78.6.1794","DOIUrl":"10.32592/ARI.2023.78.6.1794","url":null,"abstract":"<p><p>Newcastle disease (ND) is an economically significant and extremely spreadable viral illness affecting a wide variety of avian species. ND can rapidly spread within poultry farms and result in considerable economic losses for the global poultry industry. This disease is endemic in Iran, and despite intensive vaccination efforts in the poultry industry, outbreaks of ND occur unexpectedly. This study aimed to isolate the Newcastle disease virus (NDV) from poultry farms with breathing problems in Markazi province, Iran, and investigate the evolutionary relationship and molecular characteristics of the isolates during 2017-2019. To this end, tissue samples (lung, brain, and trachea) were taken from 42 broiler farms exhibiting respiratory symptoms. The samples were inoculated into 9-11-day-old embryonated eggs, and the virus was isolated from 20 (47.6%) of the 42 farms. Subsequently, RT-PCR was used to amplify partial fusion gene sequences from the new isolates. The amplified products were sequenced and compared phylogenetically to the standard pilot dataset (125 selected sequences) generated by the NDV consortium. As determined by phylogenetic analysis, all nine isolates belonged to subgenotype VII.1.1 of genotype VII and were highly similar to isolates from other parts of Iran and China. Moreover, all isolates possessed a polybasic cleavage site motif (112RRQKRF117), characteristic of virulent strains. Furthermore, the present isolates shared a high nucleotide identity (96%) with viruses previously isolated from other provinces of Iran, as determined by BLAST searches and multiple alignments. In addition, they shared a high degree of sequence similarity but were distinct from the existing NDV vaccines. Therefore, the genetic dissimilarity between current vaccine strains and circulating NDVs must be considered in vaccination programs.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"78 6","pages":"1794-1803"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the Scabies Incidence at As'ad Islamic Boarding School, Jambi City.","authors":"R Fauzah, S Suparmi","doi":"10.32592/ARI.2023.78.6.1719","DOIUrl":"10.32592/ARI.2023.78.6.1719","url":null,"abstract":"<p><p>Scabies is an infectious disease. Based on preliminary observations, numerous scabies sufferers have been found among students living in As'ad Islamic Boarding School, Jambi City, Indonesia. Scabies disease develops in humans and can be influenced by both human factors and the environment. This study aims to identify factors associated with scabies incidence at the As'ad Islamic Boarding School. This analytical observational research was conducted using a cross-sectional study design. The variables measured were scabies symptoms, knowledge, personal hygiene, contact history, humidity, and room occupancy density. The research samples consisted of 178 students at the As'ad Islamic Boarding School in Jambi City, who were selected randomly. The required data were collected by questionnaires and analyzed by Chi-square test and logistic regression. Most of the students had crowded living conditions (94.4%), low room humidity (80.8%), poor personal hygiene (66.3%), a low level of knowledge about scabies (70.8%), contact with scabies sufferers (61.8%), and symptoms of scabies (60.7%). Symptoms of scabies were related to knowledge (P=0.000), personal hygiene (P=0.000), contact history (P=0.000), humidity (P=0.000), and room occupancy density (P=0.001). The incidence of scabies in students at the As'ad Islamic Boarding School was influenced by insufficient knowledge, poor personal hygiene, history of contact, low humidity, and overcrowding.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"78 6","pages":"1719-1727"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and Risk Factors Associated with Akabane Virus Infection in Sheep and Goats in Fars Province, Iran.","authors":"M Manavian, M Hashemi, M Bakhshesh, F Tavan, M Samsami, F Saemi","doi":"10.32592/ARI.2023.78.6.1771","DOIUrl":"10.32592/ARI.2023.78.6.1771","url":null,"abstract":"<p><p>Akabane disease is an arthropod-borne viral disease that affects ruminants. This teratogenic pathogen causes severe economic losses in ruminants worldwide and in Iran; however, it has not received enough attention in Fars province, Iran. Therefore, this study aimed to determine the influence of age, gender, climate, farming system, and history of abortions on the seroprevalence of the Akabane disease in sheep and goats in Fars province. In the present study, Fars province was divided into three climates, and three cities were randomly selected from each climatic region. In each city, two epidemiologic units were selected, and all sheep and goats in each unit were sampled. Overall, 540 serum samples (391 sheep and 149 goats) were collected and examined with the commercial ELISA kit. The results showed that 83 out of 540 (15.4%) samples were seropositive and had antibodies against the Akabane virus (AKAV). The effect of gender and age on the rate of the AKAV was not significant. Animals in warm climates were 4.218 times more likely to have antibodies against the AKAV than animals in cold climates. Females were 1.32 times more likely to exhibit seropositivity. The odds of AKAV infection were higher in animals with an abortion history than in healthy animals. The findings of the present study indicated that the prevalence of the AKAV was high in small ruminants in Fars province. Therefore, it is necessary to conduct more studies to control the risk factors involved in the spread of this virus.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"78 6","pages":"1771-1778"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemistry of the Thrombin-Like Enzyme and Its Purification from Iranian Echis Carinatus Snake Venom: Its Interaction with Platelet Receptors.","authors":"N Nasri Nasrabadi, H Vatanpour, N Mohammadpour Dounighi, M Najafi, M Ahmadinejad, M A Bayatzadeh","doi":"10.32592/ARI.2023.78.6.1822","DOIUrl":"10.32592/ARI.2023.78.6.1822","url":null,"abstract":"<p><p>Snake venoms are rich in valuable substances that have medical potential in the diagnosis and treatment of hemostatic diseases. The present paper was aimed at the purification and functional characterization basis of a thrombin-like enzyme and its role in the functioning of the coagulation cascade and platelet aggregation pathway. A thrombin-like serine protease was purified from the Iranian <i>Echis carinatus</i> venom (TLIECV), employing a one-step chromatographic procedure. This peptide was collected in high yield and purity by a single chromatographic step using RP-HPLC equipped with a C₁₈ column. This peptide showed a 3000 Da molecular weight in gel-electrophoresis. Evidence in the SDS-PAGE gel has confirmed high recovery of fraction in optimal terms. Subsequently, this peptide was identified via its intact molecular mass and peptide mass fingerprint (PMF) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Multiple sequence alignments were performed by ClustalW, the Bioedit software. Molegro Data Modeller (MDM) 3.0 software was used to predict the putative tertiary structure of the peptide. The enzyme possessed fibrinogenolytic, procoagulant, and aggregation inducer properties. Moreover, the SDS-PAGE (12%) was applied to examine fibrinogenolytic function. The purified enzyme degraded the Aα chain of fibrinogen while the Bβ and γ chains were not digested. According to that, the deficient human plasma in factor X and normal human plasma were also coagulated by TLIECV, it takes part in the common and intrinsic routes of the coagulation cascade. These findings proved that TLIECV is a serine protease identical to procoagulant thrombin-like snake venom proteases; however, it specifically releases the Aα chain of bovine fibrinogen. Because of its function to make up for the deficiency of factor X and its platelet aggregation inducer property, TLIECV could be considered a molecular impact to reveal the hemostasis mechanisms.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"78 6","pages":"1822-1835"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Different amounts of Zinc Oxide Nanoparticles on the Performance and Activity of Mucosal Enzymes in Japanese Quail from 1 to 21 Days of Age.","authors":"F Ahmadi, J Ghiasi Ghaleh Kandi, F Rahimi","doi":"10.32592/ARI.2023.78.6.1787","DOIUrl":"10.32592/ARI.2023.78.6.1787","url":null,"abstract":"<p><p>This study aimed to investigate the effect of varying quantities of zinc oxide nanoparticles (ZnO NPs) on growth performance and mucosal enzyme activity in Japanese quails at an early age. Using a completely randomized experimental design, 160 one-day-old quail chicks were randomly assigned to 4 experimental treatments and each treatment contained 4 replicate pens of 10 birds. The experimental treatments included T1: control (a basal diet containing 35.2 mg Zn only ), T2, T3, and T4 containing basal diet plus 20, 40, and 60 mg ZnO NPs, respectively. Performance characteristics were recorded weekly. After 21 days, one quail was selected and slaughtered from each experimental cage with a body weight equal to the average body weight of quails in the same experimental cage. After slaughtering and opening the abdominal cavity, a 5 cm sample was taken from the jejunum of the small intestine. The jejunum sample was stored at -80°C until the measurement of alkaline phosphatase, amylase, and lipase enzymes. The results showed that live weight was higher in the T3 and T4 groups than in the control group (<i>P</i><0.05). The feed conversion ratio was also lower in birds fed with basal diets supplemented with 40 and 60 mg ZnO NPs/kg (T3 and T4, respectively), compared to control treatments (<i>P</i>>0.05). The results showed that amylase and lipase activity increased in the birds fed with 40 and 60 mg ZnO NPs/kg of the basal diet, in comparison to the control treatment; however, they were not significant (<i>P</i>>0.05). The results of this study indicated that the addition of 40 or 60 mg ZnO NPs/kg to the basal diet could be used as a supplement to improve performance traits and enhance mucosal enzyme activity in Japanese quail in the starter stage.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"78 6","pages":"1787-1793"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Characterization of Staphylococcus aureus from Raw Cow's Milk and Investigating the Effect of Bifidobacterium bifidum Probiotic Cell Free Supernatant on Their Enterotoxins Genes Expression.","authors":"H Jalaliani, Saa Anvar, K Amini, G Karim","doi":"10.32592/ARI.2023.78.6.1680","DOIUrl":"10.32592/ARI.2023.78.6.1680","url":null,"abstract":"<p><p>The present reserach aimed to detect and isolate the genes involved in the staphylococcal enterotoxins (SEs) production in strains isolated from unprocessed cow's milk and to examine the impact of <i>Bifidobacterium bifidum</i> probiotic cell-free supernatant (CFS) on their expression. Standard techniques were used for isolation and identification of Staphylococci strains in unprocessed milk. The PCR was used to identify strains carrying enterotoxin genes. The <i>B. bifidum</i> CFS was applied to strains containing the target genes, and the genes expression levels were quantified using Real-time PCR. Using 16SrDNA sequencing, the phylogenic relationship of the isolated strains was determined. Analysis revealed that bacteria such as Staphylococcus species were found in the 72% of the samples. The PCR test showed the presence of various SE superantigens, including <i>SEA</i> (16.7%), <i>SEC</i> (11.7%), <i>SED</i> (8.3%), <i>SEE</i> (6.7%), and <i>SEB</i> (1.7%) in isolated strains. The <i>B. bifidum</i> CFS had obvious antimicrobial activity against strains 24, 51, 54, and 35 of Staphylococcus species, and the minimum inhibitory concentration and minimum bactericidal concentration values for these strains treated with <i>B. bifidum</i> CFS were in the range of 31.25-125 μg/ml. Strains 51 and 24 were clustered with <i>S.aureus</i> ATCC 25923, and strains 54 and 35 were clustered with <i>S.aureus</i> ATCC 12600, respectively. The RT-PCR exhibited that probiotics CFS suppressed the expression of SEA, SEB, SEC, and SEE genes (P<0.05). The average fold change for <i>SEA</i>, <i>SEB</i>, <i>SEC</i>, and <i>SED</i> genes was -1.681, -1.28, -1.52, and -0.84, respectively. The research demonstrated that probiotic bacteria can lower enterotoxin production by downregulating the expression of SEs genes.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"78 6","pages":"1680-1689"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study of the Effects of Al(OH)₃ and AlPO₄ Adjuvants on the Production of Neutralizing Antibodies (NAbs) against Bovine parainfluenza Virus Type 3 (BPIV3) in Guinea Pigs.","authors":"R Heidary, G Nikbakht Brujeni, M Lotfi, A Hajizadeh, A R Yousefi","doi":"10.32592/ARI.2023.78.6.1779","DOIUrl":"10.32592/ARI.2023.78.6.1779","url":null,"abstract":"<p><p>Aluminum-containing adjuvants are extensively used in inactive human and animal vaccines owing to their favorable immunostimulatory and safe properties. Nonetheless, there is controversy over the effects of different aluminum salts as an adjuvant for the bovine parainfluenza virus type 3 (BPIV3) vaccine. In order to find a suitable adjuvant, we studied the effects of two adjuvants (i.e., aluminum hydroxide [Al(OH)₃] and aluminum potassium sulfate [AlPO₄]) on the production of neutralizing antibodies (NAbs) for an experimental BPIV3 vaccine. The animals under study (Guinea pigs) were randomly assigned to five groups of experimental vaccines containing Al(OH)₃ (AH), AlPO₄ (AP), Al(OH)₃-AlPO₄ mixture (MIX), commercial vaccine (COM), and control (NS). The treatment groups were immunized with two doses of vaccine 21 days apart (on days 0 and 21), and the control group received normal saline under the same conditions. The animals were monitored for 42 days, and blood samples were then taken. The results indicated that all vaccines were able to induce the production of NAbs at levels higher than the minimum protective titer (0.6). An increase in titer was observed throughout the monitoring period. Moreover, an increase in both the level and mean titer of NAbs obtained from the vaccine containing Al(OH)₃ adjuvant was significantly higher than in the other studied groups (P≤0.005). The comparison of NAbs titer in other groups did not display a significant difference. Considering the speed of rising and the optimal titer of NAbs production in the experimental vaccine, the Al(OH)₃ adjuvant is a suitable candidate for preparing a vaccine against BPIV3 for immunization.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"78 6","pages":"1779-1786"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Vaccination on Distribution and Immune Response of Avian Influenza Virus H9N2 in Coturnix coturnix.","authors":"E Golgol, M Mayahi, Z Boroomand, A Shoshtari","doi":"10.32592/ARI.2023.78.6.1746","DOIUrl":"10.32592/ARI.2023.78.6.1746","url":null,"abstract":"<p><p>Influenza viruses can multiply in quails and be transmitted to other animal species. As vaccination reduces virus shedding in chickens, the effect of the killed H9N2 avian influenza virus (AIV) on tissue distribution and virus shedding was evaluated in quails. One hundred 20-day-old quails were divided into six equal groups, kept in separate pens, and fed ad libitum. Before vaccination, blood samples were randomly collected from the wing veins. Four groups were vaccinated with the inactivated H9N2 Razi Institute vaccine at 21 days subcutaneously at the back of neck. Three weeks later, two groups were re-vaccinated. Two weeks later, at the age of 56 days, three groups were challenged with 100 μL of allantoic fluid containing 10⁵ EID₅₀ H9N2 through the oculonasal route. Blood samples were collected from quails at 42, 56, 63, and 70 days from each group to determine AIV antibodies by the hemagglutination inhibition test. Three quails were randomly selected and euthanized from each group on days 1, 3, and 6 post-inoculation (PI). Tissue samples were collected, and the RT-PCR test was performed. No clinical signs or gross lesions existed in any of the groups during the experiment. However, the virus was detected in different tissues on the first, third, and sixth days after the challenge in unvaccinated challenged birds. Virus detection was significantly more frequent in the quails vaccinated once and challenged than in the twice-vaccinated challenged group (<i>P</i>≤0.05). On the third day of PI, the virus was detected in some organs of the challenged groups. On the sixth day of PI, the virus was detected only in the lungs of two unvaccinated and once-vaccinated challenged birds. It was concluded that the vaccination of quails against AIV H9 is necessary to protect them from clinical signs, as well as respiratory tract and intestine replication. Two-time vaccination significantly protects the respiratory and intestine tracts, compared to one-time vaccination (<i>P</i>≤0.05).</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"78 6","pages":"1746-1752"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compared to Aluminum Hydroxide Adjuvant, Montanide ISA 206 VG Induces a Higher and More Durable Neutralizing Antibody Response against FMDV in Goats.","authors":"M Taghizadeh, S M Azimi, M Dabaghian","doi":"10.32592/ARI.2023.78.6.1843","DOIUrl":"10.32592/ARI.2023.78.6.1843","url":null,"abstract":"<p><p>Foot-and-mouth disease (FMD) has a high prevalence in cloven-hoofed animals. It is also highly contagious and remains a serious threat to livestock worldwide. Despite the widespread vaccination program in Iran, outbreaks of FMD continue to occur. Vaccination is one of the most effective methods of preventing FMD. The vaccines used in Iran are of the inactivated type and contain several serotypes. Since inactivated vaccines without adjuvants do not induce a high and durable antibody response, it is necessary to use adjuvants. Montanide ISA 206 VG is a mineral oil-based adjuvant that produces a water-in-oil-in-water (w:o:w) emulsion in vaccine preparations. However, a large number of manufacturers in Iran and around the world still use alum adjuvant (with or without saponin) to produce the FMD vaccine. This study used Montanide ISA 206 and alum adjuvants to administer the O2010 serotype of the FMD virus to goats. A total of six goats were divided randomly into three groups. Vaccines were administered subcutaneously twice, at a one-month interval. Blood sampling was done at different times, and the micro-neutralization method was used to measure the neutralizing antibody titer in each serum. Seven days after the second vaccination, the alum group's antibody titer was higher but not statistically significant. However, from the 28th day after the second injection until the end of the study, the Montanide ISA 206 group's antibody titer was significantly higher than that of the alum group. Six months after the second injection, the antibody titer in the ISA 206 group remained at the peak level, while in the alum group, it decreased and reached the minimum protective level. Nine months after the second injection, the antibody titer remained at its peak level in the ISA 206 group, whereas it dropped significantly in the alum group. Based on the findings, ISA 206 VG is capable of generating long-term humoral immunity in goats against the FMD serotype O2010 and could replace aluminum hydroxide adjuvants in FMD vaccine preparations.</p>","PeriodicalId":8311,"journal":{"name":"Archives of Razi Institute","volume":"78 6","pages":"1843-1851"},"PeriodicalIF":0.0,"publicationDate":"2023-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}