Antioxidative Potential and Activity of Potassium Polyacrylate and Coenzyme Q10 on Rat Hepatic Mitochondrial Permeability Transition Pores.

Q3 Veterinary
A O Adeoye, J O Olanlokun, D J Porta, J O Akinyelu, M A Rivoira, N H Garcia
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引用次数: 0

Abstract

Multiple biological activities of coenzyme Q10 have been demonstrated, opening up opportunities for research and development. However, the biological action of potassium polyacrylate and its effect on the mitochondrial permeability transition pores are both poorly understood. Therefore, this study investigated the in vitro antioxidative potential of potassium polyacrylate (PCK) and coenzyme Q10 (CoQ10) and their effects on mitochondrial permeability transition pores. In vitro antioxidant and angiotensin-converting enzyme inhibitory activities were assessed using standard methods, and lipid peroxidation was also determined. Mitochondrial swelling was evaluated as the change in absorbance under succinate-energized conditions. Cytochrome c release and mitochondrial ATPase activity were assessed. The results showed that PCK and CoQ10 significantly scavenged DPPH and nitric oxide radicals in a concentration-dependent manner and demonstrated a better ferricreducing antioxidant potential. PCK exhibited a high DPPH radical scavenging ability with the lowest IC50 value of 54.05 µg/mL while CoQ10 exhibited higher reducing power with the IC50 value of 82.14 µg/mL.Both were also found to inhibit angiotensin-converting enzyme activity. In addition, PCK and CoQ10 significantly (p<0.05) prevented lipid peroxidation, modulated the opening of mitochondrial permeability transition (mPT) pores and caused no significant release of cytochrome c. However, CoQ10 showed a mild inductive effect on mPT pores at higher concentrations. PCK and CoQ10 also increased mitochondrial ATPase activity. The results of this study suggest that both PCK and CoQ10 may be helpful in the treatment of diseases such as neurological disorders where excessive apoptosis is associated with excessive tissue degradation.

聚丙烯酸钾和辅酶Q10对大鼠肝脏线粒体通透性过渡孔的抗氧化潜力和活性。
辅酶Q10的多种生物活性已被证实,为研究和开发提供了机会。然而,聚丙烯酸钾的生物学作用及其对线粒体通透性过渡孔的影响尚不清楚。因此,本研究考察了聚丙烯酸钾(PCK)和辅酶Q10 (CoQ10)的体外抗氧化潜能及其对线粒体通透性过渡孔的影响。采用标准方法评估体外抗氧化和血管紧张素转换酶抑制活性,并测定脂质过氧化作用。线粒体肿胀通过琥珀酸激活条件下吸光度的变化来评估。测定细胞色素c释放量和线粒体atp酶活性。结果表明,PCK和CoQ10对DPPH和一氧化氮自由基的清除作用呈浓度依赖性,具有较强的还原铁的抗氧化能力。PCK具有较强的DPPH自由基清除能力,IC50最低为54.05µg/mL; CoQ10具有较强的还原能力,IC50值为82.14µg/mL。两者都能抑制血管紧张素转换酶的活性。此外,PCK和CoQ10显著(p
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来源期刊
Archives of Razi Institute
Archives of Razi Institute Veterinary-Veterinary (all)
CiteScore
1.50
自引率
0.00%
发文量
108
审稿时长
12 weeks
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