Apidologie最新文献

{"title":"The role of native and exotic bees in Cucurbita maxima var. zapallito pollination: the effects of pollination intensity and visits on commercial fruits","authors":"Mariana P. Mazzei,&nbsp;Leonardo Galetto,&nbsp;José L. Vesprini","doi":"10.1007/s13592-025-01146-3","DOIUrl":"10.1007/s13592-025-01146-3","url":null,"abstract":"<p>We conducted experimental pollinations, controlled visits, and observed pollinators’ behaviour to identify activity supporting <i>Cucurbita maxima</i> var. <i>zapallito</i> fruit production. We predict that (a) stigmas artificially pollinated with pinned native bees (<i>Eucera fervens</i>) will have more pollen grains and will produce heavier commercial fruits than those pollinated with exotic bees (<i>Apis mellifera</i>); (b) the differences in fruit weight between the native and exotic bees are expected to increase with the intensity of artificial pollination; (c) controlling <i>E. fervens</i> visits in natural pollination will show fruit trends similar to artificial pollination. Unlike <i>A. mellifera</i>, <i>E. fervens</i> reached flowers by contacting stigmas or the anthers with their abdomen, depositing more pollen grains (&gt; 80) than <i>A. mellifera</i>. Artificial pollination treatments with the two pinned-bee types resulted in fruits of similar weights regardless of pollination intensity. Conversely, in natural pollination, weight differences were observed when the number of visits increased. <i>E. fervens</i> provided at least 132 pollen grains with &gt; 3 visits: the threshold necessary for producing commercial fruits. The visitation frequency registered exceeded this minimum reaching one visit every 10 min. Other factors, such as pollination date and plant, influenced fruit production. Few visits are enough for obtaining commercial fruits: being an immature fruit, squash does not require seed development, besides species with separate flowers developed strategies to achieve rapid pollen saturation. Squash crops rely on the ecological pollination service provided by bees underscoring the conservation value of native bee species.</p>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143110128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-lived winter honey bees show unexpectedly high levels of flight activity compared to short-lived summer bees","authors":"Etienne Minaud,&nbsp;François Rebaudo,&nbsp;Fabrice Requier","doi":"10.1007/s13592-024-01144-x","DOIUrl":"10.1007/s13592-024-01144-x","url":null,"abstract":"<div><p>In temperate regions, the overwintering success of honey bee colonies, <i>Apis mellifera</i>, depends on the last generations of long-lived bees emerging in autumn, known as winter bees. While the physiological qualities of winter bees and their extended lifespan are well documented, yet literature on their flight activity performance is scarce. Here we studied the flight activity of long-lived winter honey bees and compared their performance with short-lived bees. Using radio frequency identification (RFID), we automatically monitored the number of flights, the total flight duration and the lifespan of 523 honey bees that emerged over the course of a year, including short-lived bees from spring, summer and autumn, and long-lived winter bees. We found that flight activity performance of short-lived bees decreased progressively from spring to autumn for both the number of flights and the total flight duration. Furthermore, we confirm that only a fraction of the bees emerging before winter are long-lived winter bees, with a lifespan of 143.5 ± 23.5 days (mean ± SD). With an average of 37.5 ± 44.2 flights and 12.7 ± 15.5 h of flight, we found that long-lived winter bees were substantially more active than summer and autumn short-lived bees, but performed similar activity than spring short-lived bees. We also found that a small proportion of long-lived winter bees participate in the vast majority of the flight activity of the colony. Our results suggest that the extended lifespan of long-lived winter bees does not affect their flight activity performance, probably explained by their physiological qualities.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01144-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm comet assay as a novel tool in assessing genotoxicity in high-mortality honey bee (Apis mellifera) populations","authors":"Vesela Mitkovska,&nbsp;Tsenka Chassovnikarova,&nbsp;Penka Vasileva,&nbsp;Ivan Stoyanov,&nbsp;Plamen Petrov,&nbsp;Nikolay Petkov,&nbsp;Evgeniya N. Ivanova","doi":"10.1007/s13592-024-01137-w","DOIUrl":"10.1007/s13592-024-01137-w","url":null,"abstract":"<div><p>The recent increase in mortality rates amongst honey bee colonies is a cause for concern. Assessing DNA damage in reproductive cells is crucial for species survival. This study aims to evaluate the potential of the sperm comet assay as a tool for in situ assessment of the genotoxic impact on honey bee populations with established high mortality rates. Previous studies have identified the presence of pesticide residues in bees and food stocks within the hives, indicating the existence of genotoxic agents in the localities under investigation. The values of comet assay parameters, namely Tail Intensity (TI%) and Olive Tail Moment (OTM, µm), scored in the sperm cells, increased following the mortality trend of the honey bee populations under study, providing evidence of significant DNA damage occurring during spermatogenesis. The alkaline comet assay in hemolymph confirmed the genotoxic effects observed in the sperm comet assay. The current study demonstrated for the first time that the sperm comet assay could serve as a reliable novel method for assessing genotoxicity in <i>A. mellifera</i>.\u0000</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary dopamine supplementation has no effect on ovary activity in queen-less or queen-right honeybee (Apis mellifera) workers","authors":"R. A. Knapp,&nbsp;M. R. Lovegrove,&nbsp;V. C. Norman,&nbsp;E. J. Duncan","doi":"10.1007/s13592-024-01141-0","DOIUrl":"10.1007/s13592-024-01141-0","url":null,"abstract":"<div><p>Eusociality in honeybees (<i>Apis mellifera</i>) is characterised by a reproductive division of labour, where the queen monopolises reproduction while worker reproduction is constrained. This constraint is mediated by queen mandibular pheromone (QMP), which inhibits worker ovary development through Notch signalling and possibly oocyte apoptosis. Dopamine has been implicated in regulating reproduction in worker honeybees, with prior studies suggesting that dietary dopamine enhances ovary activity in queen-less workers. This study aimed to test whether dopamine supplementation could overcome QMP-mediated reproductive constraint in worker honeybees. Using caged honeybee experiments, we administered dopamine and its precursor L-dopa at a range of concentrations, both in the presence and absence of QMP. Results showed that neither dopamine nor L-dopa supplementation affected ovary development, survival rates, or food intake, which contrasts with a previous study. These findings suggest that dopamine may not be a major factor in QMP-mediated reproductive inhibition. Instead, we suggest that the multifaceted nature of QMP’s components together with the complexity of neuroendocrine signalling makes it likely that multiple redundant mechanisms regulate worker reproduction. Future research should investigate the interplay between nutrition, dopamine and QMP components to fully understand the regulation of ovary activation in honeybee workers.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01141-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relative effectiveness of methods that sample worker honey bees to estimate Varroa destructor populations in Apis mellifera colonies","authors":"Michelle A. Taylor,&nbsp;R. Mark Goodwin,&nbsp;Heather M. McBrydie,&nbsp;Harlan M. Cox,&nbsp;Bernard C. Dominiak","doi":"10.1007/s13592-024-01143-y","DOIUrl":"10.1007/s13592-024-01143-y","url":null,"abstract":"<div><p>Honey bee (<i>Apis mellifera</i>) colonies usually survive less than 16 months if populations of parasitic mite (<i>Varroa destructor</i>) are not controlled. The timing of when to control Varroa populations is aided by using methods that estimate the number of Varroa in hives. The relative efficacy of eight methods used to calculate the number of Varroa in samples of 300 adult bees was established: icing sugar shake (78%), low-suds detergent wash (86%), ethanol wash (72%), 10-min exposure to Apistan^® (42%), ether roll (42%), a 30-s exposure to grapefruit leaf smoke (5%), a 30-s exposure to fluvalinate smoke (0%), and a 30-s exposure to tobacco leaf smoke (0%). A range of approaches were investigated to improve the efficacy and reliability of some of the methods. The following approaches improved the efficacy of four methods so that more than 95% of the Varroa were recovered: two 15-s shakes with at least 10 g of icing sugar; three 15-s washes with low-suds detergent; three 30-s washes with ethanol; and a 35-min exposure to an Apistan^® strip.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01143-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143109202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commercial honey bee keeping compromises wild bee conservation in Mediterranean nature reserves","authors":"Clément Tourbez,&nbsp;William Fiordaliso,&nbsp;Avi Bar-Massada,&nbsp;Amit Dolev,&nbsp;Denis Michez,&nbsp;Achik Dorchin","doi":"10.1007/s13592-024-01130-3","DOIUrl":"10.1007/s13592-024-01130-3","url":null,"abstract":"<div><p>Global commercial honey bee keeping for crop pollination and honey production is increasingly identified as a threat to wild bee populations. Intensive honey bee keeping is particularly alarming in Mediterranean regions that are considered global diversity hotspots for bees. Yet, the effects on the bee communities and their variation as a function of the distance to apiaries (i.e. collection of bee hives) remain poorly explored. To fill this gap, we investigated 56 sites adjacent and far (&gt; 1 km) from apiaries in three large nature reserves in Northern Israel. We explored the effects of proximity to apiaries on wild bee taxonomic and phylogenetic diversity and compared bee community composition between sites far and near to apiaries. Our records of 295 bee species include regionally restricted and undescribed species and contribute to the knowledge of Israel’s bee fauna. We found that proximity to apiaries reduced both the taxonomic and phylogenetic diversity of wild bees, and that this impact depended on individual abundance. We show that across nature reserves, 41.7 to 56.5% of the bee species diversity is concentrated in sites far from apiaries, with fewer species shared between near and far sites. Our results suggest that honey bee keeping is correlated with lower species richness in wild Mediterranean bee communities, potentially disrupting pollination processes and ultimately compromising ecosystem stability. We caution against intensive bee keeping in or near nature reserves for the conservation and safeguarding of diverse Mediterranean wild bee communities.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of exposure of larvae to boscalid at field concentrations on gene expression in honey bees","authors":"Victor Desclos le Peley,&nbsp;Carole Moreau-Vauzelle,&nbsp;Daniel Raboteau,&nbsp;Tiffany Laverre,&nbsp;Stéphane Grateau,&nbsp;Pierrick Aupinel,&nbsp;Freddie-Jeanne Richard","doi":"10.1007/s13592-024-01131-2","DOIUrl":"10.1007/s13592-024-01131-2","url":null,"abstract":"<div><p><i>Apis mellifera</i> often encounters the fungicide boscalid in agricultural landscape. In this study, we assessed the impacts of ingesting boscalid and Pictor Pro® (a commercial formulation of boscalid) at environmentally relevant doses during the larval development phase. Following chronic exposure, we measured survival, development time, and gene expression in both larvae and in 10-day-old workers. The genes analyzed were involved in immunity, detoxification, development, and mitochondrial activity pathways. We found no significant impact of boscalid in larval survival and emergence. However, genes related to cytochrome b, succinate dehydrogenase, and catalase were downregulated in larvae and adult workers exposed to boscalid, though the differences were not significant when compared to the solvent control. Late variation was observed in 10 days adult bees’ overexpression of the vitellogenin gene, which is linked to development. The effects observed with boscalid molecule were not seen with the commercially formulated product, Pictor Pro®.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo genome assembly and annotations of Bombus lapidarius and Bombus niveatus provide insights into the environmental adaptability","authors":"Vahap Eldem,&nbsp;Yusuf Ulaş Çınar,&nbsp;Selahattin Barış Çay,&nbsp;Onur Obut,&nbsp;Selim Can Kuralay,&nbsp;Mehmet Ali Balcı,&nbsp;Pınar Akbaba,&nbsp;Tuana Öğretici,&nbsp;Tunç Dabak,&nbsp;Burcu Daşer Özgişi,&nbsp;Çiğdem Özenirler,&nbsp;Gökmen Zararsız,&nbsp;Esma Gamze Aksel,&nbsp;Fatih Dikmen","doi":"10.1007/s13592-024-01140-1","DOIUrl":"10.1007/s13592-024-01140-1","url":null,"abstract":"<div><p>Bumblebees are ubiquitous, cold-adapted, primitively eusocial bees and important pollinators for crops and vegetation. However, many species are declining worldwide due to multiple factors, including human-induced habitat loss, agricultural chemicals, global warming, and climate change. In particular, future climate scenarios predict a shift in the spatial distribution of bumblebees under global warming, with some species declining and others potentially expanding. Here, we report a de novo genome assembly and annotation for <i>Bombus lapidarius</i> and <i>Bombus niveatus</i> to decipher species-specific potential genomic capacity against such environmental stressors. With harboring more than 23,000 protein-coding genes, the assembled genomes of <i>B. lapidarius</i> and <i>B. niveatus</i> are 244.44 Mb (scaffold N50 of 9.45 Mb) and 259.84 Mb (scaffold N50 of 10.94 Mb), respectively, which exhibit similar trends in terms of genome size and composition with other bumblebees. Gene family analysis reveals differences in species-specific expanded gene families. <i>B. lapidarius</i> exhibits expanded genes related to pre/postsynaptic organization, while <i>B. niveatus</i> shows a distinct expansion in gene families regulating cellular growth, aging, and responses to abiotic and biotic stressors, such as those containing SCAN domains, WD-repeats, and Ras-related proteins. Our genome-wide screens revealed positive selection on environmental stress-responsive genes such as <i>dip2</i>,<i> yme1l</i>, and <i>spg7</i> in <i>B. lapidarius</i>, whereas positive selection signatures were found in genes such as <i>myd88</i>, <i>mybbp1A</i>, and <i>rhau</i>, which are involved in environmental stress resistance for <i>B. niveatus</i>. These high-quality genome assemblies and comparative genome analysis unveil potential drivers that underlie genome evolution in bumblebees, offering valuable insights into environmental adaptation and conservation efforts.\u0000</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01140-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scientific note on mortality of a stingless bee Tetragona clavipes colony infested with clown beetles Hololepta reichii (Histeridae) and black soldier flies Hermetia illucens (Stratiomyidae) in Southeastern Brazil","authors":"Thiago S. Depintor,&nbsp;David De Jong","doi":"10.1007/s13592-024-01142-z","DOIUrl":"10.1007/s13592-024-01142-z","url":null,"abstract":"","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical exposure of honey bee queens to heat-inactivated Israeli acute paralysis virus does not protect their offspring against active infection","authors":"Prabashi Manuja Wickramasinghe,&nbsp;Chenoa NG Kaufman,&nbsp;Olav Rueppell","doi":"10.1007/s13592-024-01135-y","DOIUrl":"10.1007/s13592-024-01135-y","url":null,"abstract":"<div><p>Honey bees (<i>Apis mellifera</i>) confront a multitude of challenges to their health throughout their lifespan and have naturally evolved protective mechanisms to defend against biological stressors. Transgenerational immune priming (TGIP) is one such defense mechanism that confers protection against bacterial infections from parents to offspring. However, it is unclear whether TGIP in honey bees also protects against viral infections, which may offer a promising pathway to decrease the honey bees’ susceptibility to viral infections. We studied our hypothesis that honey bees can prime their offspring against Israeli acute paralysis virus (IAPV). We tested the prediction that the offspring of queens exposed to thermally inactivated IAPV would exhibit higher survival of an acute IAPV infection than the offspring of sham-treated queens. Based on pilot studies that compared the effects of different inoculation methods, we topically inoculated experimental queens with heat-inactivated IAPV and compared survival of an infection with active IAPV between their offspring and offspring of sham-treated control queens. IAPV infection significantly decreased offspring survival but maternal exposure to the inactive virus did not affect this outcome. Our results fail to support the notion that maternal exposure confers the same level of protection against virus infections as observed against bacterial infections, at least in this specific instant, underscoring the intricate nature of the honey bees’ transgenerational immune response. Further development of effective strategies against viral threats to improve honey bee health is needed.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01135-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}