Apidologie最新文献

{"title":"Postdiapause mating in bumblebee queens (Bombus terrestris): a novel strategy for conservation and laboratory breeding","authors":"Alena Votavová","doi":"10.1007/s13592-025-01173-0","DOIUrl":"10.1007/s13592-025-01173-0","url":null,"abstract":"<div><p>In bumblebees, successful colony establishment typically requires mating before diapause, as unmated queens were previously thought to have limited reproductive potential. However, the possibility of postdiapause mating has remained largely unexplored. This study investigated the reproductive success of <i>Bombus terrestris</i> queens by reversing the typical sequence of mating and diapause under laboratory conditions. Gynes underwent diapause for 4 or 7 months before mating with unrelated males. The results revealed high survival rates during diapause, with no significant differences between the two diapause durations. Postdiapause mating success was also high, with 82.6% of queens in the 4-month group and 77.7% in the 7-month group successfully mating. The length of diapause appeared to slightly affect the ability of nests to produce new gynes, but the difference was not statistically significant. These findings challenge the assumption that unmated gynes are inevitably constrained in their reproductive potential. If confirmed in other species, this strategy could provide a valuable tool for conservation breeding programs.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A scientific note on ‘Rapid brood decapping’—a method for assessment of honey bee (Apis mellifera) brood infestation with Tropilaelaps mercedesae","authors":"Aleksandar Uzunov,&nbsp;Irakli Janashia,&nbsp;Chao Chen,&nbsp;Cecilia Costa,&nbsp;Marin Kovačić","doi":"10.1007/s13592-025-01171-2","DOIUrl":"10.1007/s13592-025-01171-2","url":null,"abstract":"<div><p><i>Tropilaelaps mercedesae</i> is a parasitic mite species that negatively affects the health of <i>Apis mellifera</i> colonies. Recent reports show that it is spreading westwards, through Central Asia into Europe. Several field and laboratory methods have been proposed to detect <i>Tropilaelaps</i> spp. in <i>A. mellifera</i> colonies; however, most of them are recognised to be either laborious, costly or ineffective for detecting the mites, and some are even destructive to the colony. Here, we introduce a novel method for detecting and monitoring <i>T. mercedesae</i> based on the mite's characteristic biology (reduced feeding as bee pupae mature, brief dispersal phase on adult bees and agility) and the use of wax strips for decapping sealed brood area. Sealed worker brood cells at the development stage of white to purple-eyed pupae are swiftly decapped with wax strips to observe and count surfacing adult mites. The results from our study show supporting evidence of over 90% detection efficacy and brood survival, and ease of application. Therefore, we recommend the novel ‘Rapid brood decapping’ method as a reliable tool for detecting and monitoring <i>T. mercedesae</i> infestation. This method is suitable for beekeeping and research settings, being less invasive and stressful for colonies compared to other existing methods, cost-effective and quick.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A scientific note on microbial diversity in pollen stored by Melipona quadrifasciata: a comparative study of fermentation stages","authors":"Laís Calpacci Câmera,&nbsp;Yuri Ribeiro Diogo,&nbsp;Vitor Gonçalves Vital,&nbsp;Keith Dayane Leite Lira,&nbsp;Vinícius Guimarães Ferreira,&nbsp;Luciana Teresa Dias Cappelini,&nbsp;Suzan Pantaroto de Vasconcellos,&nbsp;Michelle Manfrini Morais","doi":"10.1007/s13592-025-01163-2","DOIUrl":"10.1007/s13592-025-01163-2","url":null,"abstract":"","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First evidence of large hive beetle (Oplostomus fuligineus) invasion in the Arabian region: a survey-based study of spread and future management","authors":"Yahya Al Naggar,&nbsp;Zubair Ahmad,&nbsp;Hamed A. Ghramh,&nbsp;Hatem Sharaf El-Din","doi":"10.1007/s13592-025-01166-z","DOIUrl":"10.1007/s13592-025-01166-z","url":null,"abstract":"<div><p>Large hive beetles (<i>Oplostomus fuligineus</i>, LHB) have long been recognized as significant pests of honey bee colonies, particularly across the African continent. Although LHBS are native to Sub-Saharan Africa, they have recently been recorded in North Africa, feeding primarily on young bee larvae within colonies, which leads to severe damage, especially in weaker colonies. In 2021, Abou-Shaara et al. predicted a high risk of LHB invasion into the North African and South Europe regions due to temperature variations. As a result, we performed a follow-up study based on a survey to assess the spread of LHB across the Arabian region. The questionnaire contained essential items from the global COLOSS surveys. During a two-month survey in 2024, 54 beekeepers from 11 Arabian countries responded, with Egypt, Algeria, Saudi Arabia, and Yemen contributing the most data. The infestation rate was highest in countries along the Mediterranean and the Red Sea, including Algeria, Tunisia, Egypt, Palestine, Saudi Arabia, and Yemen, indicating that LHB may favor coastal weather conditions. Nearly all beekeepers notice significant hive beetle infestations in May, June, and July. This study is the first thorough survey undertaken in the Arabian region, establishing the first recorded occurrence of LHB, confirming earlier predictions, and emphasizing the critical need for additional research and management strategies.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of zinc-methionine and Sel-Plex on the hypopharyngeal gland size, royal jelly yield and composition and the relative expression of hsp90 and trx gene in honey bees under heat stress","authors":"Khalil Rasouli-Nadergoli,&nbsp;Ali Asghar Sadeghi,&nbsp;Parvin Shawrang,&nbsp;Mohammad Chamani","doi":"10.1007/s13592-025-01159-y","DOIUrl":"10.1007/s13592-025-01159-y","url":null,"abstract":"<p>The aim of this study was to evaluate the effect of organic zinc and selenium on the antioxidant indices, acini size, royal jelly production, and relative expression of <i>hsp90</i> and <i>trx</i> genes in honey bees under heat stress. Twenty five colonies were exposed to heat stress (42 °C for at least 4 h per day) and randomly divided into five treatments. Treatments were C), the control group receiving syrup without additives and other groups receiving syrup containing 2500 µg/L of zinc (T1), 5000 µg/L of zinc (T2), 500 µg/L selenium (T3), and 1000 µg/L selenium (T4). Organic zinc increased (<i>P</i> &lt; 0.05), but selenium supplementation decreased (<i>P</i> &lt; 0.05) the activities of thioredoxin peroxidase and catalase. Organic zinc and selenium supplements decreased (<i>P</i> &lt; 0.05), the relative gene expression of <i>hsp90</i> but increased (<i>P</i> &lt; 0.05) gene expression of <i>trx</i> as compared to control. The lowest acini size was observed in C and the greatest acini size was observed in T2 (<i>P</i> &lt; 0.05). Acini size increased (<i>P</i> &lt; 0.05) as dose of organic zinc increased but not for selenium doses. It was concluded that supplementation of zinc at dose of 5000 µg/L and selenium at a dose of 500 µg /L has beneficial effects on producing royal jelly colonies.</p>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural viral infection, individual immunity, and flight performance in honey bee Apis mellifera drones","authors":"Angelina Fathia Osabutey,&nbsp;Sharif Hamdo,&nbsp;Tal Erez,&nbsp;Elad Bonda,&nbsp;Assaf Otmy,&nbsp;Victoria Soroker","doi":"10.1007/s13592-025-01157-0","DOIUrl":"10.1007/s13592-025-01157-0","url":null,"abstract":"<div><p>In honey bees, drones’ flight ability is crucial for their mating success. In this study, we assessed the flight ability of drones from three age cohorts (1–3 weeks old) by flight mill and subsequently examined their sexual development and status, viral infection by deformed wing viruses type A and B (DWV-A, DWV-B) and Israeli acute paralysis virus (IAPV) and expression of six immune genes by RT-qPCR. Sexually matured drones flew longer distances compared to immature drones. Infections of DWV-A and IAPV were common in drones, but their impact on drone flight differed. Flight distance and speed were negatively correlated with loads of IAPV and DWV-B, respectively, in 14- to 15-day-old drones, but not in 7 days old. Flight speed was negatively correlated with the expression of protein lethal 2 (<i>Pl2</i>) and peptidoglycan recognition protein S2 (<i>PGRP-S2</i>) in 14- to 15-day-old drones. These findings suggest trade-off between a drone’s flight performance and their immunity and/or the impact of virus infection on drones’ flight ability.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01157-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Varroa mites exhibit strong host fidelity despite spatial desegregation of mite and host species in Vietnam","authors":"Thi Thu Ha Nguyen,&nbsp;Yong-Chao Su,&nbsp;Adam L. Cronin","doi":"10.1007/s13592-025-01167-y","DOIUrl":"10.1007/s13592-025-01167-y","url":null,"abstract":"<div><p>Parasitic mites of the genus <i>Varroa</i> pose a serious threat to global apiculture. Host switches from native to introduced <i>Apis</i> species have occurred in multiple <i>Varroa</i> species, in some cases with devastating results. Additional host shifts and hybridisation among mite species and mite lineages on different hosts represent significant risks for apiculture and natural ecosystems. Quantifying this risk is thus an essential step toward management. The risk of hybridization among mite lineages on different hosts is likely to be at its highest in eastern Asia, where multiple host and mite lineages occur sympatrically. The available evidence, however, is somewhat contradictory, suggesting gene flow is occurring in some localities, but limited or absent in others. In this study, we elucidate the relationships between <i>Varroa</i> mites collected from <i>A. mellifera</i> and <i>A. cerana</i> in Vietnam, a region of high overlap of mite species and hosts, using a combination of genome-wide SNPs and mitochondrial sequence data. Our results indicate an absence of any new host shifts and a lack of gene flow across host species and between mite species, despite considerable overlap in mite and host species ranges. This confirms the findings of several earlier studies in this area but contrasts with evidence of hybridization in other regions, suggesting regional differences in the potential for gene flow between host/mite combinations. Nonetheless, we suggest this area remains one of high risk, because of (i) considerable overlap of mite lineages which elsewhere have been shown capable of interbreeding, (ii) evidence we find of human-mediated long-range dispersal events, which increase the chance of interaction among different mite lineages, and (iii) the persistent threat of secondary factors such as disease transmission, which may be facilitated by both of the factors above.\u0000</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01167-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term Vespa velutina nigrithorax pressure: Honey bee risk survival to alien invasion","authors":"Ana Diéguez-Antón,&nbsp;Olga Escuredo,&nbsp;M. Carmen Seijo,&nbsp;M. Shantal Rodríguez-Flores","doi":"10.1007/s13592-025-01158-z","DOIUrl":"10.1007/s13592-025-01158-z","url":null,"abstract":"<div><p>The yellow-legged hornet is an invasive species that has been established in Galicia, northwestern Spain, since 2012. This hornet shows an increased level of invasiveness, which allows it to grow and spread rapidly throughout the territory. Furthermore, the climatic characteristics of the region have contributed to their establishment, with beekeeping being the most affected sector. The study aimed to monitor the predatory behavior of Vespa velutina and its correlation with environmental conditions, as well as the honey bee survival risk of the predation. Temperatures ranging from 17 to 26 °C were the most suitable for observing the higher number of hornets. The hornets were observed in front of the colonies for 16 h a day for 11 months. The presence of five or more hornets posed a risk to the survival of the honey bee colonies. Beekeepers should manage their colonies with artificial feeding and use control methods for survival against V. velutina.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01158-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143553856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the potency, safety, and genetic makeup of Bacillus thuringiensis NBAIR BtVGa2: a promising biocontrol agent against Galleria mellonella L.","authors":"Venu H. Shamappa,&nbsp;Shylesha A. Nanjundaiah,&nbsp;Ruqiya Seegenahalli,&nbsp;Shivakumara K. Thammayya,&nbsp;Jagadeesh Patil,&nbsp;Prasannakumar M. Krishnareddy,&nbsp;Pramesh Devanna,&nbsp;Satya N. Sushil,&nbsp;Manjunatha Channappa","doi":"10.1007/s13592-025-01160-5","DOIUrl":"10.1007/s13592-025-01160-5","url":null,"abstract":"<div><p>The greater wax moth, <i>Galleria mellonella</i> L., is a noxious pest in beekeeping, causing huge economic loss by weakening the colonies and reducing honey production. Present wax moth management techniques are ineffective, potentially leading to the loss of bee colonies. Utilization of <i>Bacillus thuringiensis</i> is emerging as a sustainable practice for managing <i>G. mellonella</i>. Hence, in the present study, five <i>B</i>. <i>thuringiensis</i> isolates were isolated from soil samples and a <i>G. mellonella</i> cadaver, and all the isolates were characterized for morphological and molecular characteristics. The in vitro bioassay studies showed strain NBAIR BtVGa2 isolated from <i>G. mellonella</i> cadaver is very effective against wax moth second instar larvae with a lower LC₅₀ value of 5.40 μg/mL as compared to other <i>B</i>. <i>thuringiensis</i> strains. Biosafety studies showed NBAIR BtVGa2 is 97% safer for larvae and adults of honeybees since the strain does not consist of any hymenopteran-specific insecticidal genes. The plasmid sequencing of NBAIR BtVGa2 generated 891,714 paired-end reads with 34.13% GC content and a 0.89-Mbp genome size, along with 903 protein-coding genes with a single tRNA. The plasmid profiling of NBAIR BtVGa2 identified many insecticidal genes, viz., <i>cry</i>1Ac5, <i>cry</i>2Aa9, Zwa5A, Zwa5B, Zwa6, mpp46Ab1, and vpb4Ca1, in which mpp46Ab1 and vpb4Ca1 were novel genes with only 33.61 and 66.42% sequence similarity, respectively. The presence of these genes was validated by PCR amplification, and the structure of these insecticidal genes has also been predicted. Overall, the study identified a highly efficient strain of <i>B</i>. <i>thuringiensis</i> for the biological management of <i>G. mellonella</i>. The study also deciphered molecular mechanisms behind the efficiency of the NBAIR BtVGa2 strain. The study opened the path for the biological method of management of <i>G. mellonella</i> considering the safety of honeybees.</p><h3>Graphical Abstract</h3><p>Depicting molecular mechanism behind biocontrol potential of <i>Bacillus thuringiensis</i> strain NBAIR BtVGa2</p>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scientific note updating allelic nomenclature standards of the highly diverse complementary sex-determiner locus in honey bees","authors":"Kate E. Ihle,&nbsp;Alexandria N. Payne,&nbsp;Christine G. Elsik,&nbsp;Lelania Bilodeau","doi":"10.1007/s13592-025-01161-4","DOIUrl":"10.1007/s13592-025-01161-4","url":null,"abstract":"<div><p>Honey bees rely on haplodiploidy for sex determination. Here, diploid eggs develop into females (i.e., queens and workers) and haploid eggs develop into males (i.e., drones). This system is regulated by <i>complementary sex-determiner</i> (<i>csd</i>)—a single, multi-allelic locus with very high diversity. Honey bees heterozygous at the <i>csd</i> locus will develop into females, while hemizygosity results in normal male development. Homozygosity at <i>csd</i> results in abnormal diploid males. Diploid males are usually destroyed by the workers early in development which can negatively impact the colony population, growth, and productivity. As such, maintaining genetic diversity at <i>csd</i> is critical for the health and productivity of honey bee populations. This is reflected in the increasing number of studies examining <i>csd</i> diversity in local and global populations. However, many <i>csd</i> alleles appear in multiple studies and have been assigned several different names, complicating cross-study comparisons. In 2020, we developed a standardized nomenclature for the hypervariable region (HVR) of honey bee <i>csd</i> alleles to facilitate a better understanding of <i>csd</i> diversity within and between populations. Here, we present an updated database including all <i>csd</i> sequences deposited into GenBank since our last publication. In only the last 4 years, 370 new <i>csd</i> sequences were deposited, including 154 novel HVR sequences.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}