Apidologie最新文献_第7页

New patterns of polymorphism in the karyotypic analysis of the genus Plebeia (Hymenoptera, Apidae) Plebeia 属（膜翅目，猿科）核型分析中的多态性新模式

IF 2.4 3区农林科学

Apidologie Pub Date : 2024-07-02 DOI: 10.1007/s13592-024-01090-8

Cristiano Lula Campos, Gisele Amaro Teixeira, Denilce Meneses Lopes, Jamille de Araujo Bitencourt, Debora Diniz Bezerra, Rogério Marcos de Oliveira Alves, Hugo de Azevedo Werneck, Ana Maria Waldschmidt

{"title":"New patterns of polymorphism in the karyotypic analysis of the genus Plebeia (Hymenoptera, Apidae)","authors":"Cristiano Lula Campos, Gisele Amaro Teixeira, Denilce Meneses Lopes, Jamille de Araujo Bitencourt, Debora Diniz Bezerra, Rogério Marcos de Oliveira Alves, Hugo de Azevedo Werneck, Ana Maria Waldschmidt","doi":"10.1007/s13592-024-01090-8","DOIUrl":"10.1007/s13592-024-01090-8","url":null,"abstract":"<div>Plebeia is a taxonomically complex genus of stingless bees characterized by remarkable interspecific morphological similarity. On the other hand, cytogenetic data have been useful to resolve taxonomic uncertainties and to infer the diversification processes. Therefore, the goal of this study was to provide a karyotypic analyses in five nominal taxa of Plebeia from northeastern Brazil: P. droryana Friese (Természetrajzi Füzetek kiadja a Magyar nemzeti Muzeum 23:381–394, 1900), P. cf. mosquito Smith 1863, Plebeia aff. droryana 1, Plebeia aff. droryana 2, and Plebeia aff. fravocincta based on traditional and molecular cytogenetic methods to test their applicability in cytotaxonomy and to understand the karyoevolutionary processes in these bees. All species shared a chromosomal number of 2n = 34, except for some individuals of Plebeia aff. flavocincta that presented up to two small and heterochromatic B chromosomes. Species-specific karyotype formulae were observed while the heterochromatin segments were mainly distributed on short arms and pericentromeric regions of most chromosomes, including subtle C-banding differences in each species. The 18S rDNA sites were terminally located on pair 3 of P. droryana, P. cf. mosquito, Plebeia aff. droryana 2, and Plebeia aff. droryana 1, and on the first metacentric pair in Plebeia aff. flavocincta. These data reinforced the efficiency of cytogenetics as an informative tool to discriminate Plebeia species. The present data suggest that structural rearrangements such as inversions and duplications/deletions of heterochromatin and ribosomal clusters are the main drivers of karyotypic evolution in Plebeia.</div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141521042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex and caste effects on the vibrational sensitivity in honey bees (Apis mellifera) 蜜蜂（Apis mellifera）振动敏感性的性别和种姓效应

IF 2.4 3区农林科学

Apidologie Pub Date : 2024-06-28 DOI: 10.1007/s13592-024-01091-7

Sarah Chehaimi, Wolfgang H. Kirchner

{"title":"Sex and caste effects on the vibrational sensitivity in honey bees (Apis mellifera)","authors":"Sarah Chehaimi, Wolfgang H. Kirchner","doi":"10.1007/s13592-024-01091-7","DOIUrl":"10.1007/s13592-024-01091-7","url":null,"abstract":"<div>In the darkness of their nests, most social insect species communicate relying on chemical, tactile, electrical and mechanical signals. In honeybees, vibrational signals play a role not only in worker communication but also in communication among virgin queens in the process of swarming. Whereas the sensitivity to vibrations has been well studied in worker bees, vibrational sensitivity of queens and drones has never been investigated. We therefore studied the sensitivity to vibrations comparatively in workers, virgin and laying queens and in drones, focusing on the frequency range mainly used by virgin queens (350–500 Hz). Bees were tested behaviorally for responses to pulses of substrate borne vibrations in arenas placed on vibrational exciters. Vibrational amplitudes were measured using (LDV-calibrated) accelerometers. Real stimuli and sham stimuli were presented in a pseudorandom order. The threshold was defined as the lowest tested amplitude at which significantly more behavioral reactions were shown to real stimuli than to sham stimuli. Workers and virgin queens respond to amplitudes down to 0.25 m/s2. The thresholds of laying queens are substantially higher and they respond to minimum amplitudes of 0.55 m/s2. Drones show responses to amplitudes down to 0.6 m/s2. We conclude that sex and caste have effects on vibrational sensitivity in honeybees: virgin queens are significantly more sensitive than laying queens, indicating that a high sensitivity is crucial for survival during the process of swarming; workers are likewise highly sensitive to execute efficient everyday work; drones are significantly the least sensitive.</div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01091-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141501511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eggs of the bee Osmia lignaria endure weeks of prolonged cold weather 蜜蜂茭白的卵能经受数周的长期寒冷天气

IF 2.4 3区农林科学

Apidologie Pub Date : 2024-06-26 DOI: 10.1007/s13592-024-01085-5

James H. Cane, Ronald C. Spendal

{"title":"Eggs of the bee Osmia lignaria endure weeks of prolonged cold weather","authors":"James H. Cane, Ronald C. Spendal","doi":"10.1007/s13592-024-01085-5","DOIUrl":"10.1007/s13592-024-01085-5","url":null,"abstract":"<div>Our insights into the responses of immature stages of vernal solitary bees to the thermal challenges of spring weather mostly come from studies of cavity-nesting megachilid bees in constant temperature incubators. Most of these studies reported either the average timing of adult emergence or the general pace of immature development. In this study, we tracked the longevity and developmental fates of eggs laid by nearly 200 female Osmia lignaria (Hymenoptera: Megachilidae) occupying observation nests set outdoors in a nature preserve. In the spring of 2022, the nesting population was challenged by a prolonged period of unseasonably chilly wet weather. As a consequence, fewer nest cells were provisioned, and hatching among the 2416 eggs was delayed for as much as a month. Surprisingly, these delays incurred no ill-effects on either egg survival or subsequent larval development, both of which were comparable to the much warmer spring of 2021 (3630 eggs). The population’s 33% reproductive shortfall in 2022 was instead attributable to limited hours warm enough for flight and foraging, in part evidenced by the paucity of multi-cell days. Eggs and larvae of this bee, and probably other vernal solitary bees, readily tolerate prolonged episodes of cold springtime weather.\u0000</div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141501512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Higher early than late-season residue load of pesticides in honey bee bread in Slovakia 斯洛伐克蜜蜂面包中的农药残留量早季高于晚季

IF 2.4 3区农林科学

Apidologie Pub Date : 2024-06-24 DOI: 10.1007/s13592-024-01079-3

Martin Staroň, Abdulrahim T. Alkassab, Rastislav Sabo, Lenka Demková, Alexandra Valenčáková, Miloslav Michalko, Jaroslav Legáth, Jens Pistorius, Lucia Sabová

{"title":"Higher early than late-season residue load of pesticides in honey bee bread in Slovakia","authors":"Martin Staroň, Abdulrahim T. Alkassab, Rastislav Sabo, Lenka Demková, Alexandra Valenčáková, Miloslav Michalko, Jaroslav Legáth, Jens Pistorius, Lucia Sabová","doi":"10.1007/s13592-024-01079-3","DOIUrl":"10.1007/s13592-024-01079-3","url":null,"abstract":"<div>Bees are often exposed to pesticide residues during their foraging trips in agricultural landscapes. The analysis of in-hive stored pollen reflects the spectrum of visited plants and can be almost used to link the exposure to pesticides.In the current study, bee bread samples were collected in May and July from 17 sites located in southern Slovakia. Samples were analysed using a multi-residue pesticide analysis method for a broad spectrum of active substances and microscopic for pollen identification.Our results revealed a bee bread contamination with 19 different active substances, with fungicides being predominant. Sixteen of them are authorized in the EU, but chlorpyrifos, chlorpyrifos-methyl, and chloridazon are not. The highest concentrations for pendimethalin (1400 µg/kg), fluazifop-butyl (640 µg/kg), fenpropidin (520 µg/kg), fluopyram (130 µg/kg), and difenoconazole (95 µg/kg) were detected. The total residue load in bee bread sampled in the early season (May) was significantly higher than in the late season (July). The mean residue load of insecticides analysed in July comprised 46% of May’s load, which is alarming due to the importance of bee bread in the diet for winter-rearing bees. Moreover, results from both sampling periods showed that fungicides were positively associated with plant families Apiaceae and Papaveraceae and herbicides with Aceraceae, Salicaceae, and Brassica-type/Brassicaceae.Hence, bee bread can be considered a suitable matrix and a good bio-indicator reflecting honey bee exposure to pesticides over the season.</div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01079-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141501514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endochitinase from the Microsporidia Nosema ceranae facilitates infection in the honey bee Apis mellifera 来自陶瓷诺斯马氏小孢子虫的内切酶促进蜜蜂的感染

IF 2.4 3区农林科学

Apidologie Pub Date : 2024-06-24 DOI: 10.1007/s13592-024-01078-4

Amanda Martins da Cruz Souza, Carolina Gonçalves Santos, André Henrique Oliveira, Lenise Silva Carneiro, Matheus Tudor Cândido Santos de Resende, Leandro Licursi Oliveira, José Eduardo Serrão

{"title":"Endochitinase from the Microsporidia Nosema ceranae facilitates infection in the honey bee Apis mellifera","authors":"Amanda Martins da Cruz Souza, Carolina Gonçalves Santos, André Henrique Oliveira, Lenise Silva Carneiro, Matheus Tudor Cândido Santos de Resende, Leandro Licursi Oliveira, José Eduardo Serrão","doi":"10.1007/s13592-024-01078-4","DOIUrl":"10.1007/s13592-024-01078-4","url":null,"abstract":"<div>Bees are crucial pollinators in terrestrial ecosystems, responsible for 80% of insect-driven pollination and playing a vital role in the pollination of 75% of crops. The honey bee, Apis mellifera, is not only used in honey production but also serves as a pollinator in agriculture. However, A. mellifera faces various challenges, including exposure to pathogens such as the Microsporidia Nosema ceranae, which has been linked to decreased crop yields and colony losses. Nosema ceranae spores infect adult honey bees by penetrating the midgut lumen and invading the cytoplasm of epithelial cells, completing their life cycle. However, the midgut possesses a protective mechanical barrier called the peritrophic matrix, composed of chitin and proteins, which prevents epithelial infection. Nevertheless, N. ceranae overcomes this primary defense mechanism, though the specific mechanisms it employs to cross the peritrophic matrix and reach the midgut epithelium are not yet well understood. This study aimed to investigate the potential role of the predicted endochitinase from N. ceranae to infect bees. We tested the hypothesis that inhibiting the expression of N. ceranae endochitinase through RNA interference would impact the pathogen infection of A. mellifera. Bees treated with dsRNA targeting endochitinase, administered 12 and 24 h after spore inoculation, exhibited suppressed endochitinase gene expression and a decrease in the number of total and viable N. ceranae spores in the midgut. These results indicate that inhibiting the expression of the target gene through RNA interference affects Microsporidia infection, underscoring the importance of this enzyme in the infection process.\u0000</div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01078-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142200884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistance to Varroa destructor is a trait mainly transmitted by the queen and not via worker learning 对破坏者 Varroa 的抗性主要是由蜂王传播的，而不是通过工蜂学习传播的。

IF 2.4 3区农林科学

Apidologie Pub Date : 2024-06-20 DOI: 10.1007/s13592-024-01084-6

Stephen J. Martin, Isobel Grindrod, Georgiana Webb, Rhona Toft, Ethel Villalobos

{"title":"Resistance to Varroa destructor is a trait mainly transmitted by the queen and not via worker learning","authors":"Stephen J. Martin, Isobel Grindrod, Georgiana Webb, Rhona Toft, Ethel Villalobos","doi":"10.1007/s13592-024-01084-6","DOIUrl":"10.1007/s13592-024-01084-6","url":null,"abstract":"<div>How beekeepers can propagate the Varroa-resistant traits they have in their colonies depends on how varroa resistance, i.e. the key hygienic behavioural traits, is passed onto the next generation. This study investigates if the key hygienic traits are passed between workers via learning as is known to happen in bumble bees, or are the resistant traits encoded into the queens and thus her offspring. To test this, we re-queened known mite-resistant colonies with mite-naïve (susceptible) queens in both Hawaii and the UK. We also placed resistant queens in susceptible colonies in the UK. After 5 months in Hawaii and 12 months in the UK, mite levels in adults and brood were measured. In Hawaii, mite removal and cell recapping levels were also assessed. In both locations, the mite levels in colonies headed by suspectable (mite-naïve) queens or their daughters significantly exceeded that found in colonies headed by resistant queens or their daughters. The initial presence of resistant or suspectable workers did not affect the result. Therefore, to propagate mite-resistant traits, beekeepers only need to re-queen a colony with a locally mated queen from an established resistant population, as some UK and Hawaiian beekeepers are already doing.</div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01084-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141528883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The stingless bee Trigona fulviventris prefers sweet and salty over savory nectar 无刺蜂 Trigona fulviventris 喜欢甜味和咸味花蜜，而不是咸味花蜜

IF 2.4 3区农林科学

Apidologie Pub Date : 2024-06-17 DOI: 10.1007/s13592-024-01081-9

Gemma Nydia Villagómez, Johannes Spaethe, Sara Diana Leonhardt

{"title":"The stingless bee Trigona fulviventris prefers sweet and salty over savory nectar","authors":"Gemma Nydia Villagómez, Johannes Spaethe, Sara Diana Leonhardt","doi":"10.1007/s13592-024-01081-9","DOIUrl":"10.1007/s13592-024-01081-9","url":null,"abstract":"<div>Nectar, an important reward in plant-pollinator interactions, mainly provides sugars, but also other nutrients. Nectar sugar content is known to affect pollinator preferences, while the effect of other nectar nutrients on flower visitor choices is less well understood. To explore whether nectar nutrients affect the foraging preferences of the stingless bee Trigona fulviventris, we conducted feeding choice experiments with sucrose solution enriched with either an amino acid mixture (in high and low concentrations), sodium chloride (table salt), a fatty acid mixture, or quinine (a bitter tasting substance used as negative control) against pure sucrose solution in a lowland rainforest in Costa Rica. Bees were first trained to feeders presenting either a yellow (only sucrose solution) or a blue (sucrose solution plus tested substance) visual cue that the bees could use to associate with the solution content. They were subsequently offered a choice between the blue and yellow feeders in an unrewarded test. The bees preferred pure sucrose solutions over sucrose solutions with quinine, amino acids, or fatty acids, but not over solutions with salt. Our findings indicate that T. fulviventris is able to differentiate various nutrients in nectar and prefers pure sucrose solution or sucrose solutions with salt. Sugar and salt may therefore represent important nutrients in nectar and are likely key cues to assess nectar quality in tropical stingless bees. In contrast, other nutrients appear to compromise the bees’ taste for nectar and are likely obtained from other sources.</div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01081-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141501513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estuarine floodplains harbor greater diversity of orchid bees (Hymenoptera: Apidae: Euglossini) than mangroves in coastal Amazonia 在亚马孙沿海地区，河口冲积平原比红树林蕴藏着更丰富的兰花蜂（膜翅目：鳞翅目：Euglossini）种类

IF 2.4 3区农林科学

Apidologie Pub Date : 2024-06-11 DOI: 10.1007/s13592-024-01073-9

José J Corrêa-Neto, Juliana Hipólito, Cristiano Feitosa Ribeiro, J. Christopher Brown, Marcio Luiz de Oliveira

{"title":"Estuarine floodplains harbor greater diversity of orchid bees (Hymenoptera: Apidae: Euglossini) than mangroves in coastal Amazonia","authors":"José J Corrêa-Neto, Juliana Hipólito, Cristiano Feitosa Ribeiro, J. Christopher Brown, Marcio Luiz de Oliveira","doi":"10.1007/s13592-024-01073-9","DOIUrl":"10.1007/s13592-024-01073-9","url":null,"abstract":"<div>Variations in vegetation types in a given location, including the availability of floral resources, can directly influence the composition of orchid bee (Euglossini) communities. A diverse range of vegetation types are found along the coast of the Brazilian Amazon, with Mangrove (MANG) and Estuarine Floodplain (FE) forests being the predominant types. With the exception of dryland forests, taxonomic information and distribution of Euglossini in MANG and EF in the coastal Amazon are still incipient. Based on this, we investigated the role of these vegetation types in maintaining Euglossini bee communities on the coast of the Brazilian Amazon. We sampled bees using aromatic baits in 48 locations, 23 in EF and 25 in MANG, comparing the abundance, richness, diversity, and composition of bees. We collected a total of 1017 specimens from four genera and 22 species of Euglossini bees. Abundance and richness were, on average, greater in EF. Species composition differed statistically but was weakly explained by vegetation type. We also identified three indicator bee species for EF and one for MANG. Apparently, floristic diversity in EF is the main predictor for greater abundance and richness of Euglossini bees in the studied region. Our findings reinforce the importance of conserving native vegetation along the Amazon coast, considered one of the most threatened on the planet, to maintain the biodiversity of its pollinators.</div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141410051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The development of a loop-mediated isothermal amplification (LAMP) assay to detect American foulbrood in managed honey bee populations 开发环介导等温扩增（LAMP）测定法，以检测管理蜜蜂种群中的美洲恶臭病

IF 2.4 3区农林科学

Apidologie Pub Date : 2024-06-11 DOI: 10.1007/s13592-024-01080-w

Danielle Ackerly, Lily Tran, Travis Beddoe

{"title":"The development of a loop-mediated isothermal amplification (LAMP) assay to detect American foulbrood in managed honey bee populations","authors":"Danielle Ackerly, Lily Tran, Travis Beddoe","doi":"10.1007/s13592-024-01080-w","DOIUrl":"10.1007/s13592-024-01080-w","url":null,"abstract":"<div>Paenibacillus larvae (American foulbrood) is a lethal and arguably the most destructive and economically important notifiable bacterial disease that severely impacts brood and colonies of the Apis mellifera (Western honey bee) worldwide. Detection in apiaries requires visual inspection of clinically symptomatic hives, which is unreliable, with laboratory confirmation required for definitive diagnosis. These methods can be costly, time-consuming, and require access to specialised equipment operated by experienced personnel. Disease confirmation is essential for notifiable diseases to mitigate spread and economic damages; therefore, rapid, sensitive, and specific point of care diagnostics are critical to prevent misdiagnosis and further outbreaks. To improve diagnostic turnaround, we developed a highly sensitive and specific novel loop-mediated isothermal amplification (LAMP) assay for the detection of P. larvae (AFB-LAMP), designed to amplify a small region of the DNA gyrase subunit B gene (GyrB) with 100% specificity demonstrated against non-target bacterial species of the honey bee gut microbiome and analytical sensitivity of 5 × 10−7 ng P. larvae with detection times within 20 min. To further reduce diagnostic resources and times, a bead-beating DNA extraction method suitable for field use was optimised which resulted in an AFB-LAMP diagnostic sensitivity and specificity of 97 and 98%, respectively. Thus, this AFB-LAMP is applicable for use in the field allowing for improved disease management of an agriculturally important species.</div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01080-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141410078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity of Apis mellifera complementary sex determiner (csd) in four Australian breeding populations 澳大利亚四个繁殖种群中蜜蜂互补性决定因子（csd）的遗传多样性

IF 2.4 3区农林科学

Apidologie Pub Date : 2024-06-04 DOI: 10.1007/s13592-024-01076-6

Erica Y. Y. Mo, Patsavee Utaipanon, Tiffane Bates, John Davies, Catherine E. Grueber, Julianne Lim, Elizabeth A. Frost, Nadine C. Chapman

{"title":"Genetic diversity of Apis mellifera complementary sex determiner (csd) in four Australian breeding populations","authors":"Erica Y. Y. Mo, Patsavee Utaipanon, Tiffane Bates, John Davies, Catherine E. Grueber, Julianne Lim, Elizabeth A. Frost, Nadine C. Chapman","doi":"10.1007/s13592-024-01076-6","DOIUrl":"10.1007/s13592-024-01076-6","url":null,"abstract":"<div>Honey bee (Apis mellifera) breeding has intensified as populations experience increasing stress and pollination demand increases. Breeding programmes risk genetic diversity losses as mating is focused on a small group of individuals. Loss of diversity at the complementary sex determiner (csd) locus results in decreased viability and reduced honey production. Bees that are homozygous at csd become inviable males rather than workers. We examined csd diversity in four Australian breeding populations: a queen bee breeder from New South Wales, another from Queensland, a Western Australian breeding programme involving 11 bee breeders, and a research population at the NSW Department of Primary Industries. We found 82 unique csd alleles across the four populations, 16 of which have not been previously reported. This study provides a snapshot of csd diversity in Australia which will be useful for the national honey bee genetic improvement programme (Plan Bee).\u0000</div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01076-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141253103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0