Morphology of the hypopharyngeal gland of the stingless bee Scaptotrigona postica (Apidae: Meliponini) and the effects of pesticides

IF 2.4 3区农林科学 Q1 ENTOMOLOGY

Apidologie Pub Date : 2024-10-10 DOI:10.1007/s13592-024-01118-z

Renan Gabriel de Almeida Sousa, Pedro Vale de Azevedo Brito

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引用次数: 0

Abstract

Bees play a crucial role in pollination but are exposed to various pesticides during nectar and pollen collection, which can impact their health and pollination efficiency. This study aimed to investigate morphological and histochemical changes in the hypopharyngeal glands of Scaptotrigona postica after exposure to three chemical compounds: glyphosate, fipronil, and Tween. Nurse bees of S. postica were divided into four experimental groups and fed with sucrose syrup containing the compounds for 24 h. After the exposure period, the hypopharyngeal glands were analyzed for morphology using light microscopy and transmission electron microscopy. The hypopharyngeal glands of S. postica exhibited a tubuloacinar structure, with unicellular acini approximately 54 µm in diameter. Exposure to fipronil resulted in changes in the organization of the rough endoplasmic reticulum (RER) and increased area and sphericity of the acini compared to all other groups, as well as a higher presence of proteins in the cytoplasm compared to other pesticides, potentially due to increased food consumption. Bees exposed to glyphosate showed alterations in the mitochondria compared to all groups. The Tween group did not exhibit significant changes in cellular ultrastructure, only a smaller area and sphericity compared to the control group. The hypopharyngeal glands are highly sensitive to pesticides and are efficient bioindicators. Significant changes in these glands can compromise the maintenance of the colony.

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无刺蜂Scaptotrigona postica（Apidae：Meliponini）咽下腺的形态和杀虫剂的影响

蜜蜂在授粉过程中发挥着至关重要的作用，但在采集花蜜和花粉的过程中会接触到各种杀虫剂，这可能会影响它们的健康和授粉效率。本研究旨在调查Scaptotrigona postica暴露于草甘膦、氟虫腈和吐温三种化合物后下咽腺的形态和组织化学变化。蜜蜂分为四个实验组，分别喂食含有上述化合物的蔗糖浆 24 小时，暴露期结束后，使用光学显微镜和透射电子显微镜分析下咽腺的形态。波斯菊的下咽腺呈管状腺体结构，单细胞尖头直径约为 54 µm。与所有其他组别相比，接触氟虫腈会导致粗面内质网（RER）的组织发生变化，并增加尖头的面积和球形度，而且与其他杀虫剂相比，细胞质中的蛋白质含量更高，这可能是由于食物消耗量增加所致。与所有组别相比，接触草甘膦的蜜蜂线粒体发生了变化。吐温组的细胞超微结构没有明显变化，只是面积和球形度比对照组小。下咽部腺体对杀虫剂高度敏感，是有效的生物指示器。这些腺体的显著变化会影响蜂群的维持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Apidologie 生物-昆虫学

CiteScore

5.10

自引率

8.30%

发文量

审稿时长

3 months

期刊介绍： Apidologie is a peer-reviewed journal devoted to the biology of insects belonging to the superfamily Apoidea. Its range of coverage includes behavior, ecology, pollination, genetics, physiology, systematics, toxicology and pathology. Also accepted are papers on the rearing, exploitation and practical use of Apoidea and their products, as far as they make a clear contribution to the understanding of bee biology. Apidologie is an official publication of the Institut National de la Recherche Agronomique (INRA) and Deutscher Imkerbund E.V. (D.I.B.)