Apidologie最新文献

{"title":"Differential gene expression responsible for caste determination at both larval and adult stages of Bombus terrestris","authors":"Baodi Guo,&nbsp;Shibonage K. Mashilingi,&nbsp;Muhammad Naeem,&nbsp;Chunting Jie,&nbsp;Ziyu Zhou,&nbsp;Guiling Ding,&nbsp;Jiaxing Huang,&nbsp;Jiandong An","doi":"10.1007/s13592-024-01117-0","DOIUrl":"10.1007/s13592-024-01117-0","url":null,"abstract":"<div><p>The influence of gene expression on female caste determination in social Hymenoptera is one of the best-characterized examples of developmental polyphenism. We used mRNA-seq to profile differentially expressed genes (DEGs) between workers and queens at four development stages (early larvae: 1-day-old larvae; mid larvae: 5-day-old larvae; late larvae: 9-day-old larvae; and 7-day-old adult bee) of <i>Bombus terrestris</i>. When DEGs of different female castes were compared, it was found that there were more DEGs at the mid larval and adult stages than at other stages. Caste differentiation may be linked to candidate genes such as <i>Vitellogenin</i>, <i>Kr-h1</i>, <i>Dnmt3</i>, <i>Hexamerin</i>, <i>Yellow</i>, and <i>Chymotrypsin-2</i>. Additionally, the gene expression profiles were observed differently among the four development stages. Comparing one stage to the next showed that, more number of DEGs were found in queens than workers. In the investigation of how gene expression affects larval caste determination, WGCNA showed that five modules were closely associated with larval caste determination. The functions of genes were mainly enriched in energy metabolism and energy production within mitochondria, suggesting that the energy requirements during the larval development process varied between caste determination. The current study sheds light on the patterns of gene expression in the female caste of <i>Bombus terrestris</i>, which will be useful for future research on polyphenism through differential gene expressions.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology of the hypopharyngeal gland of the stingless bee Scaptotrigona postica (Apidae: Meliponini) and the effects of pesticides","authors":"Renan Gabriel de Almeida Sousa,&nbsp;Pedro Vale de Azevedo Brito","doi":"10.1007/s13592-024-01118-z","DOIUrl":"10.1007/s13592-024-01118-z","url":null,"abstract":"<div><p>Bees play a crucial role in pollination but are exposed to various pesticides during nectar and pollen collection, which can impact their health and pollination efficiency. This study aimed to investigate morphological and histochemical changes in the hypopharyngeal glands of <i>Scaptotrigona postica</i> after exposure to three chemical compounds: glyphosate, fipronil, and Tween. Nurse bees of <i>S. postica</i> were divided into four experimental groups and fed with sucrose syrup containing the compounds for 24 h. After the exposure period, the hypopharyngeal glands were analyzed for morphology using light microscopy and transmission electron microscopy. The hypopharyngeal glands of <i>S. postica</i> exhibited a tubuloacinar structure, with unicellular acini approximately 54 µm in diameter. Exposure to fipronil resulted in changes in the organization of the rough endoplasmic reticulum (RER) and increased area and sphericity of the acini compared to all other groups, as well as a higher presence of proteins in the cytoplasm compared to other pesticides, potentially due to increased food consumption. Bees exposed to glyphosate showed alterations in the mitochondria compared to all groups. The Tween group did not exhibit significant changes in cellular ultrastructure, only a smaller area and sphericity compared to the control group. The hypopharyngeal glands are highly sensitive to pesticides and are efficient bioindicators. Significant changes in these glands can compromise the maintenance of the colony.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scientific note: overlapping temporal distributions of drone flights of Apis laboriosa and Apis cerana in Bhutan","authors":"Tshering Nidup,&nbsp;Phuntsho Namgay Galay,&nbsp;Woesel Lhendup,&nbsp;Gard W. Otis","doi":"10.1007/s13592-024-01109-0","DOIUrl":"10.1007/s13592-024-01109-0","url":null,"abstract":"","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nesting biology of two species of oil-collecting bees of the genus Paratetrapedia s.l. (Apidae: Tapinotaspidini) in Argentina","authors":"Juan Pablo Torretta,&nbsp;Adan A. Avalos,&nbsp;Lionel E. Fernandez Pacella,&nbsp;Leopoldo J. Álvarez","doi":"10.1007/s13592-024-01114-3","DOIUrl":"10.1007/s13592-024-01114-3","url":null,"abstract":"<div><p><i>Paratetrepedia</i> is the richest genus in the tribe Tapinotaspidini and its species have a wide distribution in the Neotropical region; however, little is known about the nesting biology of its species. Here, we report information about two species: <i>Paratetrapedia</i> (<i>Lophopedia</i>) <i>nigrispinis</i> (Vachal) and<i> P</i>. (<i>Paratetrapedia</i>) <i>leucostoma</i> (Cockerell) in Argentina. Nests of both species were similar: brood cells were elongated ovals and were excavated in the wood, arranged in linear series of one to three. Brood cells were internally shiny (varnish-like), possibly due to the lining carried out by the females, and were partitioned with the sawdust obtained from the excavated cells. Our data suggest that both species showed a strong association with Malpighiaceae flowers (however, the number of brood cells analysed was very low [1 and 2 brood cells] and this fact suggests that the assumption of oligolecty can be speculative) and could have bivoltine life cycles with one rapid spring/early-summer generation, and a second generation in late-summer/autumn. These <i>Paratetrapedia</i> species shared common behavioural traits with other studied wood-nesting species of the genus, which we suggest calling them “carpenter oil-collecting bees”.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Higher early than late-season residue load of pesticides in honey bee bread in slovakia","authors":"Martin Staroň,&nbsp;Abdulrahim T. Alkassab,&nbsp;Rastislav Sabo,&nbsp;Lenka Demková,&nbsp;Alexandra Valenčáková,&nbsp;Miloslav Michalko,&nbsp;Jaroslav Legáth,&nbsp;Jens Pistorius,&nbsp;Lucia Sabová","doi":"10.1007/s13592-024-01119-y","DOIUrl":"10.1007/s13592-024-01119-y","url":null,"abstract":"","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01119-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142410629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haplotype diversity and Varroa destructor infestation patterns in commercial beekeeping operations across Southwestern Saudi Arabia","authors":"Keegan Nichols,&nbsp;Khalid A. Khan,&nbsp;Tonya Shepherd,&nbsp;Hamed A. Ghramh,&nbsp;Juliana Rangel","doi":"10.1007/s13592-024-01105-4","DOIUrl":"10.1007/s13592-024-01105-4","url":null,"abstract":"<div><p>The honey bee subspecies native to Saudi Arabia, <i>Apis mellifera jemenitica</i> Rutter, is currently being threatened by genetic pressure from exotic subspecies imported by commercial beekeepers. Uncontrolled interbreeding between native and exotic subspecies could dilute advantageous adaptations and give rise to new haplotypes that are not well suited for Saudi Arabia’s harsh climate. In this study, we analyzed the mitochondrial haplotypes and parasitization patterns by <i>Varroa destructor</i> mites in commercial beekeeping operations across Southwestern Saudi Arabia. We sampled 300 workers from five randomly chosen colonies at each of the eleven apiaries from December 2022 to February 2023. We extracted DNA from two workers per colony for analysis of the mitochondrial COI-COII region and assessed the remaining workers for mite infestation levels. We also haplotyped a subgroup of mites. All 55 colonies analyzed belonged to the <i>A. m. jemenitica</i> subspecies, which is part of the Z subgroup of the A lineage. Mitochondrial genome analysis revealed ten distinct haplotype sequences at four of the eleven locations. The average (± SEM) number of mites per 100 workers across all sites was 1.95 ± 0.96 and was below a 3% infestation threshold at nine of the eleven locations. All tested mites belonged to the Korean haplotype. The Al-Riyan, Al-Radha, and Khaitaa apiaries had both, no novel haplotypes, and the lowest <i>Varroa</i> mite infestation levels. This study could ultimately inform a colony selection process for the implementation of a breeding program aimed at improving honey bee productivity in Southwestern Saudi Arabia.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The heat is on: impact of heat waves on critical thermal maxima in larvae and adults of solitary bee Osmia bicornis (Hymenoptera: Megachilidae)","authors":"Agnieszka Gudowska,&nbsp;Dawid Moroń","doi":"10.1007/s13592-024-01112-5","DOIUrl":"10.1007/s13592-024-01112-5","url":null,"abstract":"<div><p>Extreme temperature events, such as heat waves, are increasing in frequency, magnitude, and duration. These events are believed to contribute to pollinator decline. Critical thermal maxima (CT_max) is a key physiological trait for understanding an organism’s ecology and predicting its responses to changes in climate. In this study, we investigated whether different life stages with distinct thermoregulatory behaviors differ in their CT_max in the solitary bee <i>Osmia bicornis</i>, one of the most common and important pollinators in Central Europe. Additionally, we tested the influence of excessively high temperatures, heat waves, on the CT_max in <i>Osmia bicornis</i>. We found CT_max varied among life stages, with adults exhibiting higher CT_max than larvae. Both females and males of adult bees showed a negative correlation between CT_max and body mass. Interestingly, adult bees exposed to different heat waves during their larval stage did not exhibit significant shifts in CT_max. These results suggest that bees may have limited capacity to enhance heat tolerance in response to prior heat exposure.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01112-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Main causes of producing honey bee colony losses in southwestern Spain: a novel machine learning-based approach","authors":"Eduardo José García-Vicente,&nbsp;María Benito-Murcia,&nbsp;María Martín Domínguez,&nbsp;Ana Pérez Pérez,&nbsp;María González Sánchez,&nbsp;Ismael Rey-Casero,&nbsp;Juan Manuel Alonso Rodríguez,&nbsp;Óscar Barquero-Pérez,&nbsp;David Risco Pérez","doi":"10.1007/s13592-024-01108-1","DOIUrl":"10.1007/s13592-024-01108-1","url":null,"abstract":"<div><p>Honey bees assume a pivotal role as primary pollinators, but they are currently facing a growing crisis of colony losses on a global scale. This sector is important for generating essential products, preserving ecosystems, and crop pollination. This study includes the sampling of 179 beehives from three apiaries in the traditional beekeeping area of Extremadura (Spain) vital beekeeping sector and was carried out between 2020 and 2021 using the decision trees-based model. Some studies have tried to identify the primary causative factors of this issue. However, it is insufficient because the approach disregards potential nonlinear interactions among the various factors. For this reason, through meticulous exploration of different causative factors including <i>Varroa destructor</i>, <i>Nosema ceranae</i>, Deformed Wing Virus (DWV), Chronic Bee Paralysis Virus (CBPV), and strength factors, our study employed for first time machine learning methods to identify the most important variables generating colony loss. Our analysis underscores the importance of brood levels (operculated and open), pollen and honey, <i>Varroa destructor</i> infestation, virus (DWV), and honey bee populations as key determinants of colony survival. These findings hold promise for guiding efficacious colony management strategies and underscoring the latent potential of machine-learning applications in the realm of beekeeping.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01108-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The selection traits of mite non-reproduction (MNR) and Varroa sensitive hygiene (VSH) show high variance in subsequent generations and require intensive time investment to evaluate","authors":"Lina Sprau,&nbsp;Birgit Gessler,&nbsp;Melanie Liebsch,&nbsp;Kirsten Traynor,&nbsp;Peter Rosenkranz,&nbsp;Martin Hasselmann","doi":"10.1007/s13592-024-01110-7","DOIUrl":"10.1007/s13592-024-01110-7","url":null,"abstract":"<p>The honey bee ectoparasite <i>Varroa destructor</i> is the main cause of honey bee colony losses worldwide. Over the last decades, several projects have focused on improving the robustness of <i>Apis mellifera</i> against this parasitic mite. Selection traits, such as mite non-reproduction (MNR) and <i>Varroa</i> sensitive hygiene (VSH), are favored selection factors in <i>Varroa</i> resistance projects. VSH is a trait where adult honey bees remove the <i>Varroa</i>-infested brood. During this process, the female mites are arrested in their reproductive cycle leading to a reduction of the <i>Varroa</i> population within the bee colony. From 2019 to 2022, 1402 queens were instrumentally inseminated with single or multiple drones in a breeding program. Colonies headed by these queens were established annually, and the MNR and VSH levels were analyzed. VSH was evaluated in response to cells artificially infested with <i>Varroa</i>, and colonies with high VSH values were used to generate our selected VSH stock. Despite crossing high VSH drones and queens, we measured a remarkable heterogeneity of MNR and VSH in the next generation(s), most likely due to the well-described, high recombination rate in the honey bee genome. When assessed multiple times in the same colony, great variance between measurements was observed. Detailed evaluations of daughter colonies are thus required if selection programs want to breed colonies with reliable VSH traits. This constant need to evaluate all offspring to ensure the desirable resistance traits are present results in high workloads and great expenses in selection programs. Furthermore, such large-scale breeding programs are inefficient due to high fluctuations between measurements and generations, indicating we need to develop new approaches and improved methods for assessing <i>Varroa</i> resistance.</p>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-024-01110-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142414225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low concentrations of imidacloprid affect the survival of Tetragona clavipes in a short period of exposure (24 h)","authors":"Aline A. de Oliveira,&nbsp;Lainara O. Carvalho,&nbsp;Hellem Victoria R. dos Santos,&nbsp;Luiz Ricardo G. R. de Oliveira,&nbsp;Althiéris S. Saraiva","doi":"10.1007/s13592-024-01111-6","DOIUrl":"10.1007/s13592-024-01111-6","url":null,"abstract":"<div><p>Neonicotinoid pesticides are widely used to control insect pests in agriculture and livestock production. However, they have been found to negatively affect bees, especially <i>Apis mellifera</i>. Despite this, little research has been conducted on the impact of neonicotinoids on other bee species, including native ones. This study aimed to assess the risks to native bees exposed to low concentrations of imidacloprid (IMI). The <i>Tetragona clavipes</i> (bee native to Brazil) were exposed to eight concentrations of IMI-based insecticide, through contaminated food or mist (350 µL/min) for 24 h. The LC_10-24 h, LC_50-24 h, LC_90-24 h in acute topical effect (ATE) and acute residual effect (ARE) of IMI-based insecticide against bees were calculated. The LC_10-24 h, LC_50-24 h, and LC_90-24 h in ARE were found to be more toxic compared to ATE. In summary, the study highlights that even low levels of IMI can significantly reduce bee survival within 24 h. The urgency to tackle the effects of neonicotinoid pesticides on native bee populations is evident. Preserving bees is crucial for ensuring sustainability, food security, and ecosystem integrity. Responsible pesticide management and conservation efforts are pivotal in safeguarding pollinators and upholding ecological balance.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"55 5","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142413536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}