Apidologie最新文献

{"title":"Continuous hive monitoring reveals colony growth and activity differences among mite-resistant and Italian honey bee stocks","authors":"William G. Meikle,&nbsp;Milagra Weiss,&nbsp;Vincent A. Ricigliano","doi":"10.1007/s13592-025-01188-7","DOIUrl":"10.1007/s13592-025-01188-7","url":null,"abstract":"<div><p>The choice of honey bee genetic stock represents an important management decision for beekeepers to improve resilience against biotic and abiotic stressors impacting their operations. Three different genetic stocks of honey bees (Pol-line, Russian and Italian) were monitored in southern Arizona field experiments for two successive 9-month periods (June to February). Continuous monitoring techniques and detailed hive assessments were used to measure differences in colony performance and behavior. On average, Italian colonies produced significantly more brood compared to both Pol-line and Russian bees and had significantly more foragers and adult bees on average than Pol-line colonies. However, both Pol-line and Russian colonies exhibited a faster rate of weight gain during the primary nectar and pollen flow season (June–September) and slower weight loss during the dearth season (October–February) compared to Italian colonies, which corresponded to results from published cage studies. Pol-line colonies started flight activity 10 min later on average than Italian colonies and ended daily flight activity about 30 min later on average than Russian colonies. There were no internal hive temperature differences among bee stocks, but Pol-line colonies maintained the highest carbon dioxide (CO₂) concentrations. Finally, Varroa mite levels in Pol-line and Russian colonies were significantly lower than Italian colonies and were correlated with brood area. Our findings highlight the potential advantages of Russian and Pol-line bees for use in commercial beekeeping operations. These observed stock differences could translate to improved crop pollination efficiency and reduced reliance on chemical mite controls.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01188-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145143903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A thirty-two-year (1984–2015) longitudinal analysis of honey bee disease and pathogen prevalence in the USA","authors":"Mohamed Alburaki,&nbsp;Samuel K. Abban,&nbsp;Jay D. Evans,&nbsp;Yan Ping Chen","doi":"10.1007/s13592-025-01187-8","DOIUrl":"10.1007/s13592-025-01187-8","url":null,"abstract":"<div><p>From 1984 to 2015, the Bee Disease Diagnostic Lab at the USDA-ARS Beltsville Bee Research Laboratory (MD, USA) analyzed 66,056 samples submitted for disease diagnosis, comprising 35,883 adult bees and 30,173 brood samples collected from symptomatic colonies nationwide. This dataset provided valuable insights into honey bee disease dynamics over three decades. Adult bee samples were screened for <i>Nosema</i> spp. and tracheal mite (<i>Acarapis woodi</i>). Brood samples were microscopically analyzed for the presence of both <i>Paenibacillus larvae</i> and <i>Melissococcus plutonius</i>, the causative agents of American foulbrood (AFB) and European foulbrood (EFB) diseases, respectively. Antibiotic resistance was tested in AFB-positive samples (<i>n</i> = 6,785) for tetracycline and tylosin. Longitudinal analysis revealed significant (<i>p</i> &lt; 0.001) inter-state and seasonal differences in disease prevalence. AFB was significantly more prevalent nationwide (44.71%) compared to EFB (10.01%), with a negative correlation between the two diseases (R = -0.4, <i>p</i> &lt; 0.01). <i>P. larvae</i> resistance to tetracycline and tylosin declined significantly (<i>p</i> &lt; 0.001) in later years, with national resistance averages of 42.52% and 27.78%, respectively. Additionally, positive correlations were recorded between AFB prevalence and <i>P. larvae</i> resistance to both antibiotics. <i>Nosema</i> spp. prevalence ranged from 0% to 77.9% across states, with a national average of 24.09% and significant seasonal variations (<i>p</i> &lt; 0.001). From 2008 to 2015, <i>Nosema</i> infection rates increased significantly, contrasting with a marked decline in tracheal mite prevalence since 2007 (national average of 12.48%), which was negatively correlated with <i>Nosema</i> infection (R = -0.3, <i>p</i> &lt; 0.05). This study provides unprecedented longitudinal insights into honey bee disease dynamics in the United States, highlighting the significant emergence of EFB and <i>Nosem</i>a as threats from 2008 to 2015 and a drastic reduction in tracheal mite prevalence. These findings underscore the need for continued monitoring and adaptive management strategies to protect honey bee health and ensure sustainable pollination services.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01187-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145143936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicted m6A and m5C marks in honeybee larval transcriptomes imply functional differences in caste development","authors":"Luana Bataglia,&nbsp;Flavia Cristina de Paula Freitas,&nbsp;Daniel Guariz Pinheiro,&nbsp;Zilá Luz Paulino Simões,&nbsp;Francis Morais Franco Nunes","doi":"10.1007/s13592-025-01186-9","DOIUrl":"10.1007/s13592-025-01186-9","url":null,"abstract":"<div><p>Female (diploid) honeybee (<i>Apis mellifera</i>) larvae are developmentally plastic and may become either queens or workers, depending on the nutritional stimuli received during the larval stages. Caste-specific diets activate distinct regulatory networks, leading to differential expression of coding and non-protein-coding genes, splicing variants, differences in the chromatin structures, and DNA methylation patterns, as well as variations in proteomic landscapes and the activity of key signaling pathways. Previous studies have reported the involvement of RNA epigenetics in honeybee caste fate. To further explore this and gain new insights on RNA modifications in caste diphenism, we predicted m⁶A and m⁵C RNA methylation sites in differentially expressed transcriptomes of the fourth instar larvae (L4) of queens and workers. We showed enriched functions among upregulated transcripts in L4 workers with predicted m⁶A and m⁵C sites, such as the metabolism of macromolecules and juvenile hormone synthesis. In L4 queens, these sites were predicted in upregulated transcripts that participate in mitochondrial energy metabolism, cytoskeletal organization, transport, and localization of molecules. Our findings add relevant information and novel perspectives on the potential role of the epitranscriptomic layer in the complex molecular interactions that guide the process of caste differentiation.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145164853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental stress assessment in genetically related honey bee foragers (Apis mellifera) kept in urban and rural conditions","authors":"Łukasz Nicewicz,&nbsp;Agata Wanda Nicewicz,&nbsp;Mirosław Nakonieczny,&nbsp;Marzena Stańska,&nbsp;Tomasz Stański","doi":"10.1007/s13592-025-01184-x","DOIUrl":"10.1007/s13592-025-01184-x","url":null,"abstract":"<div><p>Urban beekeeping is common and has become a permanent part of the landscape of many cities. However, there is scarce information about the health of honey bees (<i>Apis mellifera</i>) from apiaries in cities. Here, we present that the living environment of bees from urban apiaries is not indifferent to their total antioxidant capacity, immune system (defensin-2), and proteostasis (Hsp70). Still, the city does not always have only negative effects. Using an experimental system of genetically related bee colonies located in urban and rural environments, we found that genetic factors, environmental factors, or both impact the levels of the analyzed stress biomarkers. The response to environmental stress in the urban and rural areas was tissue-dependent and changed during the season. Our results will be valuable in discussing bee welfare in cities.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 3","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01184-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145164854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A commercial fungus-based biopesticide impacts survival and recognition system of a tropical social bee","authors":"Sircio Alison dos Santos,&nbsp;André Rodrigues de Souza,&nbsp;Amanda Prato,&nbsp;Bruno Gusmão Vieira,&nbsp;Denise Araujo Alves,&nbsp;Fábio Santos do Nascimento","doi":"10.1007/s13592-025-01185-w","DOIUrl":"10.1007/s13592-025-01185-w","url":null,"abstract":"<div><p>The use of fungus-based biopesticides has grown worldwide as an effective and ecologically sustainable strategy for the control of crop pests, which in turn increases the need to investigate potential harmful effects on non-target organisms in tropical agroecosystems. However, such effects of commercial formulations based on the entomopathogenic fungus <i>Beauveria bassiana</i> on stingless bees, the main pollinators of a wide range of flowering crops, remains largely unexplored. Here, we tested if Boveril^®WP, a commercial bioinsecticide, affects survival, cuticular hydrocarbon profile and social recognition in the stingless bee <i>Scaptotrigona depilis</i>. Combining exposure assay, cuticular hydrocarbon profiling, and lure presentation, we found that this mycopesticide causes the following: (1) lethal effects when topically applied on the thorax (LT₅₀ = 30 min) or abdomen (LT₅₀ = 24 h) of foragers; (2) quantitative changes in cuticular hydrocarbon profile of foragers within 12 h, containing significantly higher proportions of three linear alkanes and one methylated alkane; (3) subtle effect on social recognition by entrance guards, with lower aggression rates toward exposed foragers (nestmates or not), which may facilitate the transmission of lethal pathogens into and among colonies. This study demonstrates the potential incompatibility of a common biopesticide with a native pollinator, thus highlighting the much-needed Integrated Pest and Pollinator Management (IPPM) approaches for the best use of ecological services provided by non-target organisms.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144125851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mt-COI heteroplasmy characterization of Tetragonisca angustula (Hymenoptera: Apidae)","authors":"Natália Leite Padoan,&nbsp;Paulo Cseri Ricardo,&nbsp;Larissa Batista Cont,&nbsp;Maria Cristina Arias,&nbsp;Elaine Françoso","doi":"10.1007/s13592-025-01181-0","DOIUrl":"10.1007/s13592-025-01181-0","url":null,"abstract":"<div><p>Heteroplasmy is characterized by different mitochondrial haplotypes within the same organism. After evidence of heteroplasmy was found in mitochondrial sequences in <i>Tetragonisca angustula</i>—a bee species of great economic and ecological importance—the aim of this study was to confirm the presence of heteroplasmy in the species, analyse the frequency of synonymous and non-synonymous mutations, and classify them according to their impact on the protein’s tertiary structure. For this purpose, mitochondrial DNA was isolated from nuclear DNA in two <i>T. angustula</i> individuals, a portion of <i>COI</i> was amplified by PCR from pure mitochondrial DNA, and the different mitochondrial haplotypes were recovered through vector cloning in competent bacteria. Our results confirmed the presence of 11 and 17 different mitochondrial haplotypes in the first and second samples analysed, respectively. These different mitochondrial haplotypes are characterized by the predominance of synonymous mutations or mild and moderate non-synonymous mutations, meaning they have little impact on the protein’s tertiary structure. These results strongly support the presence of heteroplasmy in <i>T. angustula</i> and provide some insights into its characterization and inheritance.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01181-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Honey bee queen’s response to nutritional stress may depend on the stock variety","authors":"Julia D. Fine,&nbsp;Christopher Mayack,&nbsp;Michelle C. Lucadello,&nbsp;Arian Avalos,&nbsp;Elizabeth M. Walsh","doi":"10.1007/s13592-025-01182-z","DOIUrl":"10.1007/s13592-025-01182-z","url":null,"abstract":"<div><p>There are several honey bee (<i>Apis mellifera</i>) stocks with desirable traits that have been developed through selective breeding, but there are likely to be resultant trade-offs due to biological constraints or neutral evolution. The Russian honey bee (RHB) stock has higher resistance to <i>Varroa</i> mites (<i>Varroa destructor</i>) and tends to have more frequent brood rearing breaks relative to the common Italian honey bee (IHB) stock, which requires high levels of food consumption to sustain long brood rearing cycles. Here, we investigate how queens from the two stocks perform following prolonged nutritional stress in the form of chronic pollen deprivation and a 3-week recovery period. Overall, we find that IHB queens are heavier regardless of their nutritional treatment and that those that did not experience pollen dearth lay more eggs with a higher protein to egg weight ratio. While we cannot rule out the influence of external factors present in the colony setting such as pathogens, our findings suggest that IHB queen egg laying performance may be relatively higher when pollen resources are abundant, but at the same time, they have a higher level of sensitivity to nutritional stress. In addition, our results suggest that the more frequent brood rearing breaks exhibited by RHBs may not be due to reductions in queen egg laying in response to nutritional stress. We find that the best performing honey bee queen (RHB or IHB) is context dependent due to specific trade-offs in performance, which beekeepers may want to consider in the future for honey bee breeding and management.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01182-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glyphosate-based herbicide increases the number of foraging trips but does not affect the homing of Bombus terrestris","authors":"Kimmo Kaakinen,&nbsp;Satu Ramula,&nbsp;Marjo Helander","doi":"10.1007/s13592-025-01180-1","DOIUrl":"10.1007/s13592-025-01180-1","url":null,"abstract":"<div><p>The intensification of pesticide use is believed to be one of the main causes of the global decline in pollinators. The ability to forage resources effectively and return to the colony is crucial for individual eusocial bees and their colonies, and some pesticides are known to disturb this ability. Our study investigated the effects of the most widely used type of pesticide, glyphosate-based herbicides (GBHs), on the foraging and homing ability of the buff-tailed bumblebee, <i>Bombus terrestris</i> (L.) (Hymenoptera: Apidae). We conducted two experiments in which we exposed bees to field-realistic doses of GBH at colony and/or individual levels and observed their foraging activity and the homing ability of displaced bees. We found that 24-h colony-level GBH exposure increased the number of bumblebees’ foraging bouts, but it did not affect the duration of bouts or the homing ability. Regarding the homing rate, there was a marginally significant interaction between acute individual-level treatment and release distance from the colony. Even though the negligible impacts of short-term GBH exposure on homing ability are encouraging, the greater number of (possibly unnecessary) foraging bouts after colony-level GBH exposure might be costly for bumblebees.\u0000</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01180-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wild and managed bee communities in canola respond to landscape context and farm management","authors":"Rae L. Olsson,&nbsp;Vera W. Pfeiffer,&nbsp;Benjamin W. Lee,&nbsp;Liesl Oeller,&nbsp;David W. Crowder","doi":"10.1007/s13592-025-01176-x","DOIUrl":"10.1007/s13592-025-01176-x","url":null,"abstract":"<div><p>Pollinating bees promote greater yield and quality of oilseed crops like canola. Canola acreage has grown over 300% in the past decade in the Pacific Northwest region of the US, providing seasonal pulses of nectar and pollen resources that may affect bee abundance and community structure. However, because pollination by insects does not limit canola seed production, few studies have examined the biodiversity of pollinators that use these resources, or the floral traits of canola that affect pollinators. Here, we conducted surveys at canola farms across the inland Northwest USA to assess how bee biodiversity and abundance varied based on canola production practices and floral traits of canola varieties. We show that mining bees (Adrenidae) were more abundant earlier in the season and sweat bees (Halictidae) later in the season, and that bees were more abundant on farms with less floral nectar and on those in less developed landscapes. Floral traits such as larger petal size and increased nectar were correlated with lower bee abundance and diversity. However, this may reflect that petal size was associated with canola type, and that more abundant and diverse bee communities may have been more effective at removing floral nectar. While we did not find a direct effect of canola type on bees, we did find that winter canola had larger petals but lower nectar volume, which indirectly affected bee diversity and abundance. This research provides information for canola growers and land managers interested in pollinator conservation and offers a framework for future research in pollinator management.\u0000</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in pollen preferences between young worker bees and mature foragers (Apis mellifera L.)","authors":"Rocío Lajad,&nbsp;Andrés Arenas","doi":"10.1007/s13592-025-01177-w","DOIUrl":"10.1007/s13592-025-01177-w","url":null,"abstract":"<div><p>Pollen selection in <i>Apis mellifera</i> colonies is crucial for their development and productivity. Bees consume the pollen stored in the hive at early ages (mostly when they perform as nurses) but switch from consuming it to collecting it when they mature into foragers. Because of these differences in pollen use, we hypothesize that pollen preferences between young bees and foragers are not the same. In addition, we hypothesize that pollen preferences are also affected by experiences with the resource. To study differences in pollen preferences between the two groups, we compared the consumption preferences of nurse-aged workers for four monofloral pollens in the cages where they were confined, versus the foraging preferences of free-flying mature foragers for the same pollens offered in a foraging station. To study whether pollen experience affects choices, initial preferences and preferences obtained several hours after offering pollen were compared in both young bees and foragers. We observed that the pollen preferences of young bees and foragers were different and were modified by the experience. Interestingly, preferences of experienced young bees and foragers became more similar to each other than when they were naïves, a response that could be advantageous for the nutrition of early workers, who would have resources available in a proportion more similar to the one they consume.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 2","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}