mt-COI heteroplasmy characterization of Tetragonisca angustula (Hymenoptera: Apidae)

IF 2.4 3区农林科学 Q1 ENTOMOLOGY

Apidologie Pub Date : 2025-05-09 DOI:10.1007/s13592-025-01181-0

Natália Leite Padoan, Paulo Cseri Ricardo, Larissa Batista Cont, Maria Cristina Arias, Elaine Françoso

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Abstract

Heteroplasmy is characterized by different mitochondrial haplotypes within the same organism. After evidence of heteroplasmy was found in mitochondrial sequences in Tetragonisca angustula—a bee species of great economic and ecological importance—the aim of this study was to confirm the presence of heteroplasmy in the species, analyse the frequency of synonymous and non-synonymous mutations, and classify them according to their impact on the protein’s tertiary structure. For this purpose, mitochondrial DNA was isolated from nuclear DNA in two T. angustula individuals, a portion of COI was amplified by PCR from pure mitochondrial DNA, and the different mitochondrial haplotypes were recovered through vector cloning in competent bacteria. Our results confirmed the presence of 11 and 17 different mitochondrial haplotypes in the first and second samples analysed, respectively. These different mitochondrial haplotypes are characterized by the predominance of synonymous mutations or mild and moderate non-synonymous mutations, meaning they have little impact on the protein’s tertiary structure. These results strongly support the presence of heteroplasmy in T. angustula and provide some insights into its characterization and inheritance.

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膜翅目：蜂科四角蜂的mt-COI异质性研究

异质性是指同一生物体内存在不同的线粒体单倍型。在Tetragonisca angustula（一种具有重要经济和生态意义的蜜蜂物种）线粒体序列中发现了异质性的证据后，本研究的目的是确认该物种中异质性的存在，分析同义和非同义突变的频率，并根据它们对蛋白质三级结构的影响对它们进行分类。为此，我们从2个古鳗个体的细胞核DNA中分离线粒体DNA，用PCR方法从纯线粒体DNA中扩增出一部分COI，并在感态菌中通过载体克隆获得不同的线粒体单倍型。我们的结果证实，在分析的第一个和第二个样本中，分别存在11和17种不同的线粒体单倍型。这些不同的线粒体单倍型的特点是同义突变或轻度和中度非同义突变的优势，这意味着它们对蛋白质的三级结构影响很小。这些结果有力地支持了古鳗异质性的存在，并为其特征和遗传提供了一些见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Apidologie 生物-昆虫学

CiteScore

5.10

自引率

8.30%

发文量

审稿时长

3 months

期刊介绍： Apidologie is a peer-reviewed journal devoted to the biology of insects belonging to the superfamily Apoidea. Its range of coverage includes behavior, ecology, pollination, genetics, physiology, systematics, toxicology and pathology. Also accepted are papers on the rearing, exploitation and practical use of Apoidea and their products, as far as they make a clear contribution to the understanding of bee biology. Apidologie is an official publication of the Institut National de la Recherche Agronomique (INRA) and Deutscher Imkerbund E.V. (D.I.B.)