Apidologie最新文献

{"title":"Deformed wing virus–induced changes in honey bee reception and preference for pollen scents","authors":"Diego Silva,&nbsp;Felipe Becerra,&nbsp;Sebastián Salazar,&nbsp;Nolberto Arismendi,&nbsp;Juan Pablo Alveal,&nbsp;Ricardo Ceballos,&nbsp;Nelson Zapata,&nbsp;Marisol Vargas","doi":"10.1007/s13592-025-01196-7","DOIUrl":"10.1007/s13592-025-01196-7","url":null,"abstract":"<div><p>Honey bees (<i>Apis mellifera</i> L.) play a crucial role in pollination, and their olfactory system is essential for food collection and source selection. This study evaluates how the deformed wing virus (DWV), particularly its variant A, affects the olfactory sensitivity and behavioral responses of bees (10 to 20 days old) to volatile compounds from different pollen sources. We collected and analyzed the volatile fractions of three types of pollen (polyfloral and two monofloral) using dynamic HeadSpace and gas chromatography–mass spectrometry (GC–MS). The chemical analysis revealed differences in volatile compound profiles among the pollen types, including the presence of benzaldehyde, lilac alcohol, and 1–4 cineole, which are known to impact honey bee olfaction. Behavioral assays using a Y-olfactometer showed that while non-inoculated bees (N-DWV) responded to the aromas of all pollens in higher proportions, DWV-inoculated bees (I-DWV) exhibited significant reductions in behavioral responses. Specifically, I-DWV bees showed lower response rates, and a higher proportion of non-responding individuals compared to N-DWV bees. Notably, I-DWV bees were more attracted to the aroma of <i>Eucryphia cordifolia</i> when exposed simultaneously to the aroma of <i>Rubus ulmifolius</i>. This preference may be related to specific volatiles emitted by <i>E. cordifolia</i> that signal higher nutritional or medicinal value. Future analyses focused on the antiviral activity of this pollen could help clarify whether such attraction is associated with adaptive mechanisms against viral infection. Additionally, electrophysiological recordings indicated that DWV-A infection decreases olfactory sensitivity, particularly in response to complex odors. This decline in olfactory function and behavioral preferences could compromise foraging efficiency and overall colony health. These findings highlight the impact of DWV-A on honey bee sensory and behavioral processes, raising concerns about broader implications for colony survival and pollination services.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145142103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method for delivery of exogenous nucleic acids during the non-ovipositional phase in honeybees","authors":"Linxuan Xie,&nbsp;Huali Song,&nbsp;Yaohui Li,&nbsp;Yongyan He,&nbsp;Xiaomei Peng,&nbsp;Xiangyou Tang,&nbsp;Mingyu Song,&nbsp;Jinshan Xu","doi":"10.1007/s13592-025-01200-0","DOIUrl":"10.1007/s13592-025-01200-0","url":null,"abstract":"<div><p>Honeybees are essential global pollinators and key model organisms for studying insect social behavior. Genetic manipulation is crucial for understanding honeybee biology, but current methods rely on microinjecting early-stage embryos, which is technically challenging, inefficient, and often results in high embryo mortality. In this study, we identified AcQP, an ovary-targeting peptide from the Asian honeybee (<i>Apis cerana</i>), capable of delivering DNA specifically to ovarian cells and transmitting it to progeny via the laying queen. Notably, AcQP also targets the ovaries of <i>Apis mellifera</i> and other non-<i>Apis</i> species, including silkworms and mosquitoes. By integrating AcQP with the Gal4- upstream activation sequences system, we successfully introduced exogenous DNA into queen bee ovarian cells via abdominal spiracle injection. This study established an efficient ovary–targeted DNA delivery method, representing a significant advancement in honeybee genetic engineering.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145144362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pollen nutrition shapes demographic performance and gene expression in a bumblebee (Bombus eximius)","authors":"Dun-Yan Wang,&nbsp;Ming‐Chih Chiu,&nbsp;Chia Chang Hsieh,&nbsp;Mei-Hwa Kuo,&nbsp;Ming-Cheng Wu","doi":"10.1007/s13592-025-01197-6","DOIUrl":"10.1007/s13592-025-01197-6","url":null,"abstract":"<div><p>Bumblebees (<i>Bombus</i> spp.) are essential pollinators in a wide range of ecosystems, including agricultural systems. Addressing the critical knowledge gap in species-specific nutritional requirements, which leads to inconsistent colony development in native bumblebees like <i>Bombus eximius</i>, this study investigated the effects of pollen diet on microcolony development and reproduction. We examined the effects of three monofloral pollen diets, namely, <i>Brassica napus</i>, <i>Camellia sinensis</i>, and <i>Zea mays</i>, on worker egg-laying, drone larval development, lifespan, and gene expression. Our findings revealed that pollen nutritional quality significantly affected reproductive output, including the preoviposition period (time to first egg) and oviposition periods (duration of egg-laying), as well as offspring development in bumblebees. Worker bees fed <i>C. sinensis</i> and <i>B. napus</i> pollen exhibited significantly higher expression of the <i>vitellogenin</i> (encoding a yolk protein precursor crucial for oocyte development and egg production) and <i>hexamerin</i> (encoding a major storage protein vital for development and amino acid reserves) genes in their abdomens than did those fed <i>Z. mays</i> pollen. Microcolonies fed <i>C. sinensis</i> pollen demonstrated the highest reproductive success, the shortest mean semi-generation time, and the longest lifespan, indicating its superior nutritional quality for <i>B. eximius</i>. These results highlight the critical role of pollen nutrition in bumblebee health and suggest that <i>C. sinensis</i> pollen could enhance the performance of managed bumblebee colonies.\u0000</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145143973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of different constant temperatures on the survival of honey bee, Apis mellifera (Hymenoptera: Apidae)","authors":"Muhammad Sarmad,&nbsp;Pavel Ryšánek","doi":"10.1007/s13592-025-01191-y","DOIUrl":"10.1007/s13592-025-01191-y","url":null,"abstract":"<p>Honey bees play a crucial role as beneficial insects for producing honey and pollinating crops. <i>Apis mellifera</i> (Linnaeus) (Hymenoptera: Apidae) is one of the most important species of honey bees globally. Insects experience various temperature stresses in their natural habitat that differ from their typical temperature range. Being an important pollinator, the current study is designed to investigate the effect of temperature stress during summer and winter on bees’ survival, recovery rate, and longevity. To check their sensitivity, the worker bees were exposed to summer (31 to 19 °C) and winter (12 to 0 °C) temperatures. Different temperatures in summer were significantly influenced the survival rate and the time required to achieve 50% mortality. The 50% mortality was observed in 13.79 days at 31 °C and gradually decreased with the decrease in temperature. The recovery rate and longevity of the bees decreased, when exposed to cold temperatures, 12 to 0 °C for 1, 2, and 3 h. Both aspects eventually decreased with the increase in exposure duration. Based on the results, it is concluded that the survival rate, recovery rate, and longevity of the bees were greatly affected by the change in temperature. </p>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01191-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145142904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why do queen bumblebees emerge from hibernation during weekends?","authors":"Joan Díaz-Calafat,&nbsp;Jaime Luna-Santamaría","doi":"10.1007/s13592-025-01192-x","DOIUrl":"10.1007/s13592-025-01192-x","url":null,"abstract":"<div><p>Climate-driven shifts in bumblebee emergence and flowering time can disrupt plant reproduction and affect pollinator health. To study such mismatches, accurate phenological data is crucial but challenging to collect, especially for pollinator hibernation emergence. Citizen science is increasingly being used to expand the spatial and temporal scope of data collection in research. Nevertheless, these data can be biased due to different reasons. We found that 33.14% of records in Europe and 32.47% in North America were gathered on weekends – exceeding what would be expected by random chance and showing opposite patterns to bumblebee museum specimen records. Bias also affected queen emergence date and varied by species, suggesting that species-specific traits may mediate the bias extent. We also present a case study showing how adjusting for day-of-the-week effects can change the statistical significance of temporal trends in bumblebee emergence dates. We thus recommend including the day of the week in statistical models to account for temporal biases. Our findings highlight the importance of correcting temporal biases in citizen science data to ensure accurate evaluations of ecological responses to climate change.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01192-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145142647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Living in harmony with nature: the key to resilience of honeybees (Apis mellifera) in Africa","authors":"Haftom Gebremedhn,&nbsp;Regis Lefebre,&nbsp;Dirk C. de Graaf","doi":"10.1007/s13592-025-01193-w","DOIUrl":"10.1007/s13592-025-01193-w","url":null,"abstract":"<div><p>Managed honeybees play a crucial role in enhancing agricultural crop production and plant biodiversity conservation and ensuring food security. However, beekeepers, especially those in the Northern Hemisphere, are experiencing high honeybee colony losses, mainly due to the ectoparasitic mite <i>Varroa destructor</i>. This could threaten our future food security. The presence of <i>V. destructor</i> has also been reported in Africa; however, honeybees are less affected by the mite. Moreover, local beekeepers do not consider the mite as a threat to their colonies, and colonies survive without mites’ treatment. Mite-resistant honeybee populations have also been reported in various parts of Europe, but they are limited in number and confined to specific regions. Therefore, understanding why mite-resilient honeybee populations are flourishing in many African countries is crucial for developing effective management strategies to improve honeybee health worldwide. Hence, this review synthesizes existing knowledge about the factors and traits that allow mite-resilient honeybee populations in Africa to flourish and aims to translate the insights gained from Africa into more effective approaches for managing the impact of <i>V. destructor</i> worldwide. The results suggest that African-derived honeybees are resilient to a broad set of threats including <i>V. destructor</i> because they live in harmony with nature. Resilience is attributed to a more bee-friendly environment, to better balanced colony densities, to beekeepers’ management practices, and to the bee’s behavioural adaptation and physiological and genetic traits. Nature-based beekeeping practices that allow natural selection to operate and maintain the genetic diversity of local honeybee subspecies are the key to their resilience against <i>V. destructor</i>.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01193-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145142646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trace element levels in Italian honeybees and wild plants: which factors matter?","authors":"Gloria Isani,&nbsp;Cecilia Rudelli,&nbsp;Giulia Andreani,&nbsp;Micaela Fabbri,&nbsp;Chiara Suanno,&nbsp;Luigi Parrotta,&nbsp;Stefano Del Duca","doi":"10.1007/s13592-025-01194-9","DOIUrl":"10.1007/s13592-025-01194-9","url":null,"abstract":"<div><p>Honey bees (<i>Apis mellifera</i> L.) can be considered interesting biosamplers for monitoring the contaminants they may accumulate as a function of distance and time since they are exposed to these compounds either directly or indirectly. The aim of this research was to investigate how the levels of essential and non-essential trace elements in honey bees and plants are altered by hive management (organic or conventional), agricultural practices, and seasonal conditions. The levels of iron, zinc, copper, lead, and cadmium were determined in honey bees and wild plants from six different apiaries located in four Italian regions using atomic absorption spectrometry. Iron was the most abundant essential trace element, followed by zinc, while copper was an order of magnitude less abundant. Honey bees from conventional farms had significantly higher copper levels than those from organic farms. Regarding the non-essential elements, only honey bee samples from apiary 6 (conventional) showed cadmium levels of around 0.1 µg/g wet weight, and only some samples from apiary 4 (conventional) showed an average lead level of 0.83 µg/g wet weight. Among the factors tested, the season (spring and summer) and the apiary management were critical factors affecting content of trace elements in honey bees. In particular, the season is the one that has the greatest influence on element content, highlighting the possible adverse effects of climate change on honey bee health.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145142436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New highly discordant chromosome number and cytogenetic characterization in Trigona (Apidae, Meliponini)","authors":"Anderson Medeiros Dalbosco,&nbsp;Adriane Barth,&nbsp;Marco Antonio Costa","doi":"10.1007/s13592-025-01189-6","DOIUrl":"10.1007/s13592-025-01189-6","url":null,"abstract":"<div><p><i>Trigona</i> comprises 35 species, of which 25 occur in Brazil. Of these, 12 species have been cytogenetically investigated and most showed the chromosome number 2n = 34, except for <i>Trigona braueri</i>, which showed 2n = 32, due to a probable numerical reduction by chromosome fusion. In this study, <i>Trigona pellucida</i> was cytogenetically analyzed by classical and molecular techniques (Giemsa staining, CMA₃/DAPI fluorochrome staining and FISH using 18S rDNA, telomeric and microsatellite probes). New chromosome numbers, n = 10 and 2n = 20 and the diploid karyotypic formula 2 k = 14 M + 4SM + 2A were described. These new chromosome numbers were quite divergent from the other <i>Trigona</i> and such intrageneric variation was unexpected in the tribe Meliponini. CMA₃/DAPI fluorochromes staining showed CMA₃ positive segments in all chromosomes of <i>T</i>. <i>pellucida</i>, which was also divergent from other <i>Trigona</i> species. In situ hybridization with 18S rDNA probes occurred on the short arm of both chromosomes of the ninth pair, which also coincided with CMA₃⁺ regions. Microsatellite probes showed markings on all chromosomes, and in seven pairs these markings occurred in the terminal regions of both arms. FISH with telomeric probes showed regular markings in the terminal regions of the chromosomes. The present result with <i>Trigona pellucida</i> was surprising, and pose more challenges for understanding the karyotypic evolution of stingless bees than previously assumed.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145142178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing potential viral transmission between honeybees and hive-infesting ants using novel per-hive co-detection indices","authors":"Ignacio Rivas Fontan,&nbsp;Fernanda N. Gonzalez,&nbsp;Joaquin Moja,&nbsp;Cecilia Ferrufino,&nbsp;Daniel Zanola,&nbsp;Bruno Scally,&nbsp;Luis Calcaterra,&nbsp;Maria José Dus Santos,&nbsp;Roxana Josens","doi":"10.1007/s13592-025-01190-z","DOIUrl":"10.1007/s13592-025-01190-z","url":null,"abstract":"<div><p>Understanding which ant species interacting with honeybees can transmit or acquire viruses is crucial for managing honeybee health. Our objective was to develop a new methodology using two indices, based on a per-hive approach, to identify ant-virus combinations with high transmission potential (Matching Index) and to assess the relative risk posed to honeybees (Risk-to-Bee Index). Our survey in apiaries in Buenos Aires Province, Argentina, revealed that honeybee hives more infested by ants had smaller colonies. The Argentine ant, <i>Linepithema humile,</i> was among the top three ant species infesting hives. Ant brood and queens showed higher virus detection rates compared to workers, increasing virus prevalence in ants. Four viruses were detected in <i>L. humile</i>, but only deformed wing virus (DWV) was more prevalent in honeybees in hives with <i>L. humile</i>. Argentine ants tested negative for chronic bee paralysis virus, which was common in <i>Camponotus</i> species. In our study, per-hive indices suggest that Argentine ants would transmit DWV and black queen cell virus to honeybees, while honeybees would transmit DWV and acute bee paralysis virus to Argentine ants and <i>Camponotus mus</i>. Importantly, although our indices are simpler than previous tools to evaluate interspecific virus transmission, they do not provide definitive conclusions. However, the suggestions they offered align with all existing empirical data, highlighting their robustness. Thus, this approach provides a novel tool for prioritizing research on high-risk virus transmission between honeybees and ants, emphasizing its potential impact on honeybee management.\u0000</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145145265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating interaction between genotype and microbiome profile in Irish honeybees","authors":"Dara Meehan,&nbsp;Dalimil Bujdoš,&nbsp;Paul W. O’Toole","doi":"10.1007/s13592-025-01183-y","DOIUrl":"10.1007/s13592-025-01183-y","url":null,"abstract":"<div><p>Previous work from our group identified distinct microbiome profiles in thriving versus non-thriving honeybee colonies in southwest Ireland, and highlighted seasonality, rather than location, as a significant factor in microbiome dynamics. Ireland harbours a relatively pure population of <i>Apis mellifera mellifera</i>, a subspecies in decline across mainland Europe, that is threatened by introgression from imported honeybee types such as the Buckfast hybrid and C-lineage honeybees. In this pilot study, we identified mitotypes of 30 honeybee colonies across southern Ireland and sequenced the nuclear genome of 9 colonies to assess hybridization levels. We profiled the microbiome of these colonies to investigate if mitotype interacted with the microbiome. Of the 30 colonies tested, 18 were M-mitotypes, but 9 C- and 3 A-mitotypes were also detected. Nuclear genome SNP analysis revealed that M-mitotype colonies were relatively pure, displaying little to no introgression, whereas A-mitotypes displayed a hybridised genome predominantly composed of M-lineage SNPs. C-mitotype colonies also displayed varying levels of M-lineage SNPs. Microbiome composition did not differ significantly across mitotypes, with individual colony being the most significant determinant of microbiome diversity. These findings provide further evidence that the Irish honeybee population is predominantly composed of native M-lineage honeybees, though its genetic integrity is threatened by the presence of imported lineages. While this native population does not possess a unique microbiome compared to that of other lineages, these results further our understanding of factors which determine the microbiome composition of honeybee colonies in Ireland.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 4","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13592-025-01183-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145144736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}