Impact of exposure of larvae to boscalid at field concentrations on gene expression in honey bees

IF 2.4 3区农林科学 Q1 ENTOMOLOGY

Apidologie Pub Date : 2025-01-22 DOI:10.1007/s13592-024-01131-2

Victor Desclos le Peley, Carole Moreau-Vauzelle, Daniel Raboteau, Tiffany Laverre, Stéphane Grateau, Pierrick Aupinel, Freddie-Jeanne Richard

{"title":"Impact of exposure of larvae to boscalid at field concentrations on gene expression in honey bees","authors":"Victor Desclos le Peley, Carole Moreau-Vauzelle, Daniel Raboteau, Tiffany Laverre, Stéphane Grateau, Pierrick Aupinel, Freddie-Jeanne Richard","doi":"10.1007/s13592-024-01131-2","DOIUrl":null,"url":null,"abstract":"<div><p><i>Apis mellifera</i> often encounters the fungicide boscalid in agricultural landscape. In this study, we assessed the impacts of ingesting boscalid and Pictor Pro® (a commercial formulation of boscalid) at environmentally relevant doses during the larval development phase. Following chronic exposure, we measured survival, development time, and gene expression in both larvae and in 10-day-old workers. The genes analyzed were involved in immunity, detoxification, development, and mitochondrial activity pathways. We found no significant impact of boscalid in larval survival and emergence. However, genes related to cytochrome b, succinate dehydrogenase, and catalase were downregulated in larvae and adult workers exposed to boscalid, though the differences were not significant when compared to the solvent control. Late variation was observed in 10 days adult bees’ overexpression of the vitellogenin gene, which is linked to development. The effects observed with boscalid molecule were not seen with the commercially formulated product, Pictor Pro®.</p></div>","PeriodicalId":8078,"journal":{"name":"Apidologie","volume":"56 1","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Apidologie","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s13592-024-01131-2","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Apis mellifera often encounters the fungicide boscalid in agricultural landscape. In this study, we assessed the impacts of ingesting boscalid and Pictor Pro® (a commercial formulation of boscalid) at environmentally relevant doses during the larval development phase. Following chronic exposure, we measured survival, development time, and gene expression in both larvae and in 10-day-old workers. The genes analyzed were involved in immunity, detoxification, development, and mitochondrial activity pathways. We found no significant impact of boscalid in larval survival and emergence. However, genes related to cytochrome b, succinate dehydrogenase, and catalase were downregulated in larvae and adult workers exposed to boscalid, though the differences were not significant when compared to the solvent control. Late variation was observed in 10 days adult bees’ overexpression of the vitellogenin gene, which is linked to development. The effects observed with boscalid molecule were not seen with the commercially formulated product, Pictor Pro®.

查看原文本刊更多论文

幼虫暴露于田间浓度的苯硫醚对蜜蜂基因表达的影响

蜜蜂在农业景观中经常遇到杀菌剂boscalid。在这项研究中，我们评估了在幼虫发育阶段以环境相关剂量摄入boscalid和Pictor Pro®（一种boscalid的商业配方）的影响。在长期暴露后，我们测量了幼虫和10天大的工蜂的存活率、发育时间和基因表达。分析的基因涉及免疫、解毒、发育和线粒体活性途径。我们没有发现黄芩碱对幼虫存活和羽化的显著影响。然而，细胞色素b、琥珀酸脱氢酶和过氧化氢酶相关基因在暴露于boscalid的幼虫和成虫中均下调，但与溶剂对照相比差异不显著。在10天内观察到成年蜜蜂卵黄原蛋白基因的过表达，这与发育有关。在商业配方产品Pictor Pro®中，没有观察到与boscalid分子观察到的效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Apidologie 生物-昆虫学

CiteScore

5.10

自引率

8.30%

发文量

审稿时长

3 months

期刊介绍： Apidologie is a peer-reviewed journal devoted to the biology of insects belonging to the superfamily Apoidea. Its range of coverage includes behavior, ecology, pollination, genetics, physiology, systematics, toxicology and pathology. Also accepted are papers on the rearing, exploitation and practical use of Apoidea and their products, as far as they make a clear contribution to the understanding of bee biology. Apidologie is an official publication of the Institut National de la Recherche Agronomique (INRA) and Deutscher Imkerbund E.V. (D.I.B.)