Annals of Surgical Treatment and Research最新文献

{"title":"Safety and effectiveness of direct oral anticoagulants in fragile patients with venous thromboembolism: a retrospective cohort observational study.","authors":"Hojong Park, Sang Jun Park, Hyangkyoung Kim","doi":"10.4174/astr.2025.108.3.168","DOIUrl":"10.4174/astr.2025.108.3.168","url":null,"abstract":"<p><strong>Purpose: </strong>The use of direct oral anticoagulants (DOACs) is challenging in fragile patients, including those with cancer, chronic kidney disease (CKD), and old age. We aimed to compare the safety of DOACs in terms of bleeding complications in these patients.</p><p><strong>Methods: </strong>Using hospital data from 2013 to 2019, we compared the risk of bleeding and major bleeding, including intracranial bleeding, any bleeding requiring transfusion, and all-cause bleeding, in patients with venous thromboembolism (VTE) who were naïve to DOAC (n = 12,369) and warfarin (n = 4,123). Hazard ratios (HRs) for the clinical outcomes were analyzed using Cox regression analysis, with warfarin as a reference.</p><p><strong>Results: </strong>The study included 4,078 eligible patients, predominantly female (54.1%), with a mean age of 62.5 years. DOACs were the primary treatment in 74.1% of the patients. DOAC treatment was associated with lower all-cause mortality compared to warfarin (HR, 0.799; 95% confidence interval [CI], 0.707-0.904). Although rates of recurrent VTE or major bleeding did not significantly differ between the groups, DOAC-treated patients had lower bleeding risk (HR, 0.562; 95% CI, 0.393-0.805; P = 0.002). The individual DOAC drugs did not differ significantly in terms of composite outcomes, recurrence, or bleeding events.</p><p><strong>Conclusion: </strong>DOAC showed comparable outcomes with warfarin in the fragile patient population.</p>","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"108 3","pages":"168-176"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of admission base excess in postoperative outcomes of aortic dissection patients: a retrospective cohort analysis.","authors":"Huanan Liu, Hua Lu, Xiaoshen Zhang","doi":"10.4174/astr.2025.108.3.158","DOIUrl":"10.4174/astr.2025.108.3.158","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this retrospective study was to evaluate the relationship between admission base excess and clinical outcomes in postoperative patients with aortic dissection.</p><p><strong>Methods: </strong>Clinical data were extracted from the MIMIC-IV (Medical Information Mart for Intensive Care IV) database. The association between admission base excess and mortality in postoperative patients with aortic dissection was assessed using multivariate Cox regression and Kaplan-Meier survival analysis. Subgroup analysis and receiver operating characteristic (ROC) curve analysis were employed to evaluate the predictive performance of base excess for in-hospital, 30-day, 90-day, and 1-year mortality.</p><p><strong>Results: </strong>A total of 196 patients were categorized into the normal base excess (-3 to +3 mmol/L) group and abnormal base excess (<-3 or >+3 mmol/L) group. Multivariate Cox regression analysis revealed that arterial base excess was a significant predictor of all-cause mortality across all periods. Subgroup analyses showed no significant interaction effects. The area under the ROC curve for base excess ranged from 0.640 to 0.745, indicating comparable predictive performance to existing scoring tools.</p><p><strong>Conclusion: </strong>Arterial base excess measured at admission is an effective and accessible predictor of mortality in patients with aortic dissection following surgical treatment.</p>","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"108 3","pages":"158-167"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A single center experience on clinical outcome of fundoplication in pediatric patients: a retrospective cohort study.","authors":"Yuyoung Oh, Joong Kee Youn, Hee-Beom Yang, Hyun-Young Kim, Dayoung Ko","doi":"10.4174/astr.2025.108.3.177","DOIUrl":"10.4174/astr.2025.108.3.177","url":null,"abstract":"<p><strong>Purpose: </strong>The study aimed to evaluate the characteristics and operative-related factors in children who underwent fundoplication, analyze surgical outcomes categorized by disease entity and surgical indication, and identify prognostic factors for reoperation risk.</p><p><strong>Methods: </strong>A total of 109 pediatric patients who underwent fundoplication between 2008 and 2022 were retrospectively analyzed. Patients were grouped by disease entity and surgical indication. Underlying diseases, comorbidities, sex, gestational age, birth weight, preoperative symptoms, and operation-related factors were examined. Outcomes were classified as short-term and long-term adverse events. We investigated differences in clinical outcomes according to disease entity and surgical indication. Then we statistically identified preoperative predictors for the risk of reoperation.</p><p><strong>Results: </strong>The most common disease entity was neurological impairment (n = 92). Pulmonary comorbidity (42.2%) and aspiration/regurgitation (87.2%) were the most common. Most surgeries were performed laparoscopically (86.2%). There were 12 short-term and 25 long-term adverse events, with long-term events occurred more frequently in the neurological impairment (NIP) group compared to the non-NIP group (P = 0.04). None of the factors showed a significant relationship with the risk of reoperation.</p><p><strong>Conclusion: </strong>Neurologically impaired children were more likely to experience long-term adverse events post-fundoplication. However, no significant predictors for reoperation risk were identified.</p>","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"108 3","pages":"177-185"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing recurrent laryngeal nerve localization during transoral endoscopic thyroid surgery using augmented reality: a proof-of-concept study.","authors":"Moon Young Oh, Yeonjin Choi, Taesoo Jang, Eun Kyung Choe, Hyoun-Joong Kong, Young Jun Chai","doi":"10.4174/astr.2025.108.3.135","DOIUrl":"10.4174/astr.2025.108.3.135","url":null,"abstract":"<p><strong>Purpose: </strong>During transoral endoscopic thyroidectomy, preserving the recurrent laryngeal nerve (RLN) is a major challenge because visualization of this nerve is often obstructed by the thyroid itself, increasing the risk of serious complications. This study explores the application of an augmented reality (AR) system to facilitate easier identification of the RLN during transoral endoscopic thyroidectomy.</p><p><strong>Methods: </strong>Three patients scheduled for transoral endoscopic thyroidectomy were enrolled in this proof-of-concept study. Preoperative computed tomography scans were used to create an AR model that included the thyroid, trachea, veins, arteries, and RLN. The model was overlaid onto real-time endoscopic camera images during live surgeries. Manual registration of the AR model was performed using a customized controller. The model was aligned with surgical landmarks such as the trachea and common carotid artery. Manual registration accuracy was assessed using the Dice similarity coefficient (DSC) to evaluate the alignment between the real RLN and the RLN of the AR model.</p><p><strong>Results: </strong>The 3 patients included were female (mean age, 33.3 ± 15.7 years), and the mean tumor size was 1.0 ± 0.3 cm. All patients underwent transoral endoscopic thyroidectomy of the right lobe. Final histopathological diagnoses comprised 2 papillary thyroid carcinomas and one follicular adenoma. The manual registration accuracy was 0.60, 0.70, and 0.57 for patients 1, 2, and 3, respectively, with a mean value of 0.6 ± 0.1.</p><p><strong>Conclusion: </strong>The application of an AR system during transoral endoscopic thyroidectomy proved feasible and demonstrated potential for improving the localization of anatomical structures, particularly the RLN, as indicated by a moderate DSC.</p>","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"108 3","pages":"135-142"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ulcerative colitis-associated colorectal neoplasm is increasing as a surgical indication in the biologics era: a retrospective observational study of 20 years of experience in a single tertiary center.","authors":"Hyo Jun Kim, Seung-Bum Ryoo, Jin Sun Choi, Han-Ki Lim, Min Jung Kim, Ji Won Park, Seung-Yong Jeong, Kyu Joo Park","doi":"10.4174/astr.2025.108.3.150","DOIUrl":"10.4174/astr.2025.108.3.150","url":null,"abstract":"<p><strong>Purpose: </strong>We aimed to identify changes in surgical indications in patients with ulcerative colitis (UC) in the biologics era in a single tertiary center.</p><p><strong>Methods: </strong>In this retrospective observational study, 108 patients with UC who underwent abdominal surgery for UC at Seoul National University Hospital from 2000 to 2021 were included. We compared the total number of patients undergoing UC before and after the introduction of biologic therapy.</p><p><strong>Results: </strong>Of the 108 patients with UC (male, 59 and female, 49; mean age, 46.8 years), 30 (27.8%) underwent surgery for neoplasms and 78 (72.2%) for medical intractability without neoplasms. The duration between diagnosis and surgery varied significantly (126.00 months <i>vs.</i> 60.50 months, P = 0.001). A significant difference was also noted in the surgical indications according to time (P = 0.02). Between 2000 and 2010, 12 patients (19.4%) underwent surgery for UC with neoplasms and 50 (80.6%) for UC without neoplasms, while between 2011 and 2021, 18 (39.1%) and 28 patients (60.9%) underwent surgery for UC with and without neoplasms, respectively.</p><p><strong>Conclusion: </strong>Since 2011, when biological agents were covered by insurance in South Korea, there has been a relative increase in the incidence of surgical indications for neoplasia cases. Focusing on closely monitoring individuals with long-term UC for neoplasms is necessary.</p>","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"108 3","pages":"150-157"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A randomized controlled trial comparing liquid skin adhesives and staplers for surgical wound management.","authors":"Hyeon Woo Bae, Seung Yoon Yang, Ga Yoon Ku, Sohye Lee, Eun-Joo Jung, Seulkee Park, Yoon Bin Jung, Jihong Kim, Byung Soh Min","doi":"10.4174/astr.2025.108.3.143","DOIUrl":"10.4174/astr.2025.108.3.143","url":null,"abstract":"<p><strong>Purpose: </strong>Despite the widespread use of liquid skin adhesives (LSA), concerns persist regarding the increase in wound care costs. This study aimed to investigate the cost-effectiveness of LSA for surgical wound management.</p><p><strong>Methods: </strong>In this prospective, open-label, single-center randomized controlled trial, adults aged 19 years and older who were scheduled for elective minimally invasive colorectal surgeries were included. The participants were randomly divided into 2 groups: an n-butyl cyanoacrylate skin adhesive was used in the experimental group (LSA group), while a surgical skin stapler was employed in the control group (stapler group). The primary outcome measure was the sum of the total time required for wound management.</p><p><strong>Results: </strong>A total of 58 patients were randomly assigned to 2 groups, with 29 patients in each group. The findings revealed comparable wound complication rates in the 2 groups (8 out of 29 in the LSA group <i>vs.</i> 5 out of 29 in the stapler group, P = 0.530). Notably, the LSA group had a significantly shorter wound management time (median 235 seconds <i>vs.</i> 1,201 seconds, P < 0.001) and similar wound management cost (median US dollar [USD] 50.6 <i>vs.</i> USD 54.6, P = 0.529) compared to the stapler group. Subgroup analysis showed that the LSA group had a shorter management time for uncomplicated wounds and a lower cost for complicated wounds.</p><p><strong>Conclusion: </strong>LSA not only provides a safe alternative but also offers a resource-efficient option for wound management compared to staplers.</p>","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"108 3","pages":"143-149"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143622963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scaffold implantation <i>vs.</i> intravenous delivery: a comparative preclinical animal study evaluating peroxisome proliferator-activated receptor gamma coactivator 1-alpha adipose-derived stem cells in liver fibrosis treatment.","authors":"Joseph Ahn, Jung Hyun Park, Ho Joong Choi, Dosang Lee, Ha-Eun Hong, Ok-Hee Kim, Say-June Kim","doi":"10.4174/astr.2025.108.3.186","DOIUrl":"10.4174/astr.2025.108.3.186","url":null,"abstract":"<p><strong>Purpose: </strong>Regenerative medicine is expected to offer an alternative to liver transplantation for treating liver diseases in the future, with one significant challenge being the establishment of an effective stem cell administration route. This study assessed the antifibrogenic effects of adipose-derived stem cells (ASCs) in a liver fibrosis mouse model, focusing on 2 methods of delivery: intravenous injection and scaffold implantation.</p><p><strong>Methods: </strong>An extracellular matrix mimic scaffold was utilized for culturing peroxisome proliferator-activated receptor gamma coactivator 1-alpha-overexpressing ASCs (tASCs). These scaffolds, laden with tASCs, were then implanted subcutaneously in mice exhibiting liver fibrosis. In contrast, the Cell groups received biweekly intravenous injections of tASCs for 4 weeks. After 4 weeks, tissue samples were harvested from the euthanized mice for subsequent analysis.</p><p><strong>Results: </strong>Real-time PCR and Western blot analyses on liver tissues, focusing on markers like alpha-smooth muscle actin (α-SMA), matrix metalloproteinase-2, and transforming growth factor-beta 1 (TGF-β1), showed that both delivery routes substantially lowered fibrotic and inflammatory markers compared to controls (P < 0.05), with no significant differences between the routes. Histological examinations, along with immunohistochemical analysis of α-SMA, collagen type I alpha, and TGF-β1, revealed that the scaffold implantation approach resulted in a greater reduction in fibrosis and lower immunoreactivity for fibrotic markers than intravenous delivery (P < 0.05).</p><p><strong>Conclusion: </strong>These findings indicate that delivering tASCs via a scaffold could be more effective, or at least similarly effective, in treating liver fibrosis compared to intravenous delivery. Scaffold implantation could offer a beneficial alternative to frequent intravenous treatments, suggesting its potential utility in clinical applications for liver disease treatment.</p>","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"108 3","pages":"186-197"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The safety and anti-adhesive effect of acellular dermal matrix application after thyroid surgery: a multicenter randomized controlled trial.","authors":"Kwangsoon Kim, Young Jun Chai, Mira Han, Sang-Wook Kang, Ji-Sup Yun","doi":"10.4174/astr.2025.108.2.71","DOIUrl":"10.4174/astr.2025.108.2.71","url":null,"abstract":"<p><strong>Purpose: </strong>Postoperative adhesions following thyroid surgery can lead to multiple complications that significantly impact quality of life. The use of an acellular dermal matrix (ADM) adhesion barrier device has been proposed as a potential solution to reduce the risk of such adhesions. This study aimed to evaluate the safety and anti-adhesive effect of an ADM in patients undergoing thyroid surgery.</p><p><strong>Methods: </strong>In this multicenter randomized controlled trial, patients undergoing thyroid surgery were randomly assigned to receive either ADM (n = 42) or no ADM (n = 39) during surgery. The primary outcome was the Swallowing Impairment Score (SIS-6), measured 6 weeks after surgery and compared between groups. Secondary outcomes included intergroup comparisons of the SIS-6, the Voice Handicap Index (VHI)-10, and the Glasgow-Edinburgh Throat Scale (GETS) at baseline, and 2, 6, and 18 weeks after surgery.</p><p><strong>Results: </strong>At week 6, the mean SIS-6 scores were 4.0 ± 4.1 and 3.3 ± 4.2 in the ADM and control groups, respectively, which was not significantly different. Both groups showed similar postoperative improvements in SIS-6, VHI-10, and GETS scores over time, without significant differences between groups at any time point, indicating that the ADM did not reduce the incidence of postoperative adhesions or alter the course of recovery compared to the control group.</p><p><strong>Conclusion: </strong>Although application of the ADM is safe for use in patients undergoing thyroid surgery, it did not produce a clinically significant advantage in preventing postoperative adhesions. Future research should focus on identifying specific patient populations or surgical scenarios where the use of the ADM may be beneficial.</p>","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"108 2","pages":"71-78"},"PeriodicalIF":1.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The power of platelet-rich plasma on operated pilonidal disease: a prospective randomized controlled trial.","authors":"Bourak Chousein, Engin Olcucuoglu, Ismail Oskay Kaya","doi":"10.4174/astr.2025.108.2.124","DOIUrl":"10.4174/astr.2025.108.2.124","url":null,"abstract":"<p><strong>Purpose: </strong>Pilonidal sinus disease is a chronic inflammatory disease that affects approximately 1% of the population. Although many treatment methods have been described, there is no consensus on the exact treatment method. This study was conducted to evaluate the wound healing-promoting effect of platelet-rich plasma (PRP) in patients treated using the unroofing and curettage technique.</p><p><strong>Methods: </strong>A total of 140 patients diagnosed with pilonidal sinus disease were included in the study. The patients were randomized into 2 groups; one group was followed up with a standard dressing after the operation, and the patients in the other group were treated with PRP in addition to the standard dressing. In this study, the wound healing rate between the 2 groups was determined as the primary objective.</p><p><strong>Results: </strong>In postoperative follow-up, a difference in wound healing rate was detected on postoperative day 10 (P = 0.007). While the average wound healing time of the patients in the control group was 41.1 ± 11.0 days (median, 40; range, 20-65), it was 23.6 ± 8.5 days (median, 25; range, 6-45) in the PRP group (P < 0.001). It was determined that the wound closure time of patients in the PRP group was faster.</p><p><strong>Conclusion: </strong>In our prospective randomized study, we found that PRP significantly increased the wound healing rate and patient comfort, with recurrence rates below 1% and minimal pain. We think that it should be the first-choice method before operations that cause extensive tissue loss, such as flap surgery.</p>","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"108 2","pages":"124-134"},"PeriodicalIF":1.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic anticancer effects of mitochondria-targeting peptide combined with paclitaxel in breast cancer cells: a preclinical study.","authors":"Juneyoung Ahn, Ok-Hee Kim, Seongeon Jin, Ja-Hyoung Ryu, Dosang Lee, Woo-Chan Park, Say-June Kim","doi":"10.4174/astr.2025.108.2.108","DOIUrl":"10.4174/astr.2025.108.2.108","url":null,"abstract":"<p><strong>Purpose: </strong>Mitochondria-accumulating amphiphilic peptide (Mito-FF) was designed to selectively target mitochondria in cancer cells and enhance anticancer effects through its unique structure. Mito-FF consists of (1) diphenylalanine, a β-sheet-forming building block critical for self-assembly; (2) triphenylphosphonium, a mitochondrial targeting moiety guiding the peptide to mitochondria; and (3) pyrene, a fluorescent probe enabling visualization of its accumulation and self-assembly. This study evaluates the anticancer efficacy of Mito-FF in breast cancer cells and explores its combination with paclitaxel, a standard therapy for breast cancer, focusing on its modulation of the epithelial-mesenchymal transition (EMT) pathway.</p><p><strong>Methods: </strong><i>In vitro</i> and <i>in vivo</i> experiments were performed using MCF-7 and MDA-MB-231 breast cancer cell lines and their respective xenograft models. Cell viability, migration, EMT marker expression, and apoptosis-related proteins were analyzed.</p><p><strong>Results: </strong>Mito-FF demonstrated superior inhibition of cell viability and migration compared to paclitaxel alone in both cell lines. Combination therapy with Mito-FF and paclitaxel resulted in enhanced reduction of cell viability and migration. EMT markers were significantly modulated, with decreased mesenchymal markers (Snail and vimentin) and increased epithelial marker (E-cadherin) following combination treatment. Furthermore, the combination therapy synergistically elevated pro-apoptotic markers such as poly (adenosine diphosphate-ribose) polymerase and reduced anti-apoptotic markers such as myeloid cell leukemia 1. <i>In vivo</i> experiments revealed a marked reduction in tumor volume with combination therapy, accompanied by the highest expression levels of E-cadherin and pro-apoptotic marker Bim.</p><p><strong>Conclusion: </strong>Mito-FF, designed for mitochondrial targeting and visualization, exhibited potent anticancer effects when combined with paclitaxel, in the breast cancer cells.</p>","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"108 2","pages":"108-123"},"PeriodicalIF":1.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}