Protective effects of taxifolin against oxidative stress and apoptosis in liver ischemia-reperfusion injury: an experimental animal study.

IF 1.2 4区医学 Q3 SURGERY

Annals of Surgical Treatment and Research Pub Date : 2025-07-01 Epub Date: 2025-07-02 DOI:10.4174/astr.2025.109.1.53

Hüseyin Bilge, Eda Yildizhan

{"title":"Protective effects of taxifolin against oxidative stress and apoptosis in liver ischemia-reperfusion injury: an experimental animal study.","authors":"Hüseyin Bilge, Eda Yildizhan","doi":"10.4174/astr.2025.109.1.53","DOIUrl":null,"url":null,"abstract":"Purpose: Ischemia and reperfusion (I/R) can cause significant functional impairments in cells and tissues depending on the duration of ischemia. To date, many alternative treatments have been utilized to address this damage. We aimed to research the protective efficacy of taxifolin (Tax), a natural antioxidant, against liver I/R injury in terms of biochemical, histopathologic, and immunohistochemical parameters.Methods: The rats utilized in our study were randomly chosen and divided into four equal groups. Group 1 (n = 7), sham group received 1 mL saline orally; group 2 (n = 7), Tax group; Group 3 (n = 7), I/R group; group 4 (n = 7), Tax + I/R group as designated. The entire study was performed under general anesthesia with protection of animal welfare. Malondialdehyde (MDA) and interleukin-6 (IL-6) were analyzed from blood samples. Proapoptotic (B-cell lymphoma 2 [Bcl-2]-associated X protein [Bax]) and antiapoptotic (Bcl-2) amendments in cells were reviewed.Results: MDA grades were lower in the Tax+I/R group compared to the I/R group. Likewise, IL-6 grades were lower in the Tax + I/R group, histologic examinations showed that Bax proteins were less expressed in the Tax + IR group, and Bcl-2 proteins were intensely expressed in the Tax group.Conclusion: Taxifolin applied before liver I/R demonstrated favorable effects on inflammation, oxidative stress, and proapoptotic changes.","PeriodicalId":8071,"journal":{"name":"Annals of Surgical Treatment and Research","volume":"109 1","pages":"53-60"},"PeriodicalIF":1.2000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12270924/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Surgical Treatment and Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4174/astr.2025.109.1.53","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/2 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"SURGERY","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: Ischemia and reperfusion (I/R) can cause significant functional impairments in cells and tissues depending on the duration of ischemia. To date, many alternative treatments have been utilized to address this damage. We aimed to research the protective efficacy of taxifolin (Tax), a natural antioxidant, against liver I/R injury in terms of biochemical, histopathologic, and immunohistochemical parameters.

Methods: The rats utilized in our study were randomly chosen and divided into four equal groups. Group 1 (n = 7), sham group received 1 mL saline orally; group 2 (n = 7), Tax group; Group 3 (n = 7), I/R group; group 4 (n = 7), Tax + I/R group as designated. The entire study was performed under general anesthesia with protection of animal welfare. Malondialdehyde (MDA) and interleukin-6 (IL-6) were analyzed from blood samples. Proapoptotic (B-cell lymphoma 2 [Bcl-2]-associated X protein [Bax]) and antiapoptotic (Bcl-2) amendments in cells were reviewed.

Results: MDA grades were lower in the Tax+I/R group compared to the I/R group. Likewise, IL-6 grades were lower in the Tax + I/R group, histologic examinations showed that Bax proteins were less expressed in the Tax + IR group, and Bcl-2 proteins were intensely expressed in the Tax group.

Conclusion: Taxifolin applied before liver I/R demonstrated favorable effects on inflammation, oxidative stress, and proapoptotic changes.

查看原文本刊更多论文

taxifolin对肝缺血再灌注损伤氧化应激和细胞凋亡的保护作用：实验动物研究。

目的：缺血再灌注（Ischemia and reperfusion, I/R）可引起细胞和组织的显著功能损伤，这取决于缺血的持续时间。迄今为止，已经采用了许多替代疗法来解决这种损害。本研究旨在从生化、组织病理学和免疫组化等方面研究天然抗氧化剂taxifolin （Tax）对肝脏I/R损伤的保护作用。方法：随机选取实验用大鼠分为4组。1组（n = 7），假手术组口服生理盐水1 mL；第二组（n = 7），税务组；第3组（n = 7）， I/R组；第4组（n = 7），指定Tax + I/R组。在保护动物福利的情况下，整个研究在全身麻醉下进行。分析血液样本中的丙二醛（MDA）和白细胞介素-6 （IL-6）。对细胞中促凋亡（b细胞淋巴瘤2 [Bcl-2]-相关X蛋白[Bax]）和抗凋亡（Bcl-2）修饰进行了综述。结果：与I/R组相比，Tax+I/R组MDA分级较低。同样，Tax + I/R组IL-6等级较低，组织学检查显示，Tax + IR组Bax蛋白表达较少，而Tax组Bcl-2蛋白表达强烈。结论：在肝I/R前应用紫杉醇对炎症、氧化应激和促凋亡改变有良好的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Annals of Surgical Treatment and Research SURGERY-

CiteScore

2.30

自引率

7.10%

发文量

期刊介绍： Manuscripts to the Annals of Surgical Treatment and Research (Ann Surg Treat Res) should be written in English according to the instructions for authors. If the details are not described below, the style should follow the Uniform Requirements for Manuscripts Submitted to Biomedical Journals: Writing and Editing for Biomedical Publications available at International Committee of Medical Journal Editors (ICMJE) website (http://www.icmje.org).