Annals of Clinical Biochemistry最新文献_第8页

Inosine attenuates glycolysis in erythrocytes via glucose transporter inhibition. 肌苷通过葡萄糖转运蛋白抑制红细胞糖酵解。

IF 1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-03-02 DOI: 10.1177/00045632261433354

Yukio Kume, Motohiro Ohkubo, Naru NaKatuka, Sayaka Aritake-Okada, Naoyuki Yoshikawa, Teruhiko Yoshida, Hideaki Isago, Makoto Kurano

{"title":"Inosine attenuates glycolysis in erythrocytes via glucose transporter inhibition.","authors":"Yukio Kume, Motohiro Ohkubo, Naru NaKatuka, Sayaka Aritake-Okada, Naoyuki Yoshikawa, Teruhiko Yoshida, Hideaki Isago, Makoto Kurano","doi":"10.1177/00045632261433354","DOIUrl":"10.1177/00045632261433354","url":null,"abstract":"BackgroundBlood glucose concentrations decrease after blood collection. We recently developed novel blood collection tubes containing inosine added to sodium fluoride (NaF; FI tubes), which effectively inhibits post-collection blood glucose decline. However, the underlying mechanism remains unclear. In this study, we examined the mechanism by which inosine inhibits blood glucose consumption by erythrocytes and assessed glucose transporter (GLUT) activity.MethodsATP levels were measured in erythrocytes treated with inosine, and metabolomic changes were analyzed using GC-MS. In addition, glucose uptake tests were performed.ResultsIn patients' blood samples, FI tubes suppressed the post-collection decline in blood glucose levels more effectively than conventional NaF tubes, regardless of baseline blood glucose levels. FI tubes attenuated the time-dependent decrease in ATP levels; however, similar to that in conventional NaF tubes, ATP levels in erythrocytes in FI tubes were nearly zero after 4 h. Metabolic analysis demonstrated a decrease in the levels of glucose and glycolytic metabolites, such as 2-phosphoglycerate and phosphoenolpyruvate, in inosine-treated erythrocytes. Glucose uptake assays revealed that inosine significantly inhibited glucose uptake, indicating suppression of GLUT activity.ConclusionsInosine inhibits glucose uptake in erythrocytes primarily via the suppression of GLUT activity. It also inhibits erythrocyte hemolysis by temporarily maintaining intracellular ATP levels.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"45632261433354"},"PeriodicalIF":1.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147324476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of a reference interval for calculated globulin on the Roche platform. 罗氏平台计算球蛋白参考区间的建立。

IF 1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-03-01 Epub Date: 2025-07-29 DOI: 10.1177/00045632251367264

Colleen Flannery, Alison Griffin, John Quinn, Clodagh Loughrey, Shari Srinivasan

{"title":"Establishment of a reference interval for calculated globulin on the Roche platform.","authors":"Colleen Flannery, Alison Griffin, John Quinn, Clodagh Loughrey, Shari Srinivasan","doi":"10.1177/00045632251367264","DOIUrl":"10.1177/00045632251367264","url":null,"abstract":"BackgroundCalculated globulin, based on direct measurement of total protein and albumin, can be a useful addition to the routinely requested liver profile. However, a reference interval, established using CLSI EP28-A3c recommended direct methods, is lacking for the commonly used Roche method [albumin bromocresol green (BCG), total protein (biuret)].MethodsThis direct reference interval study was carried out between January and March 2024, based on Roche methods for total protein (biuret) and albumin (BCG). Reference individuals comprised 310 highly selected adults from primary care, ages ranging from 16 to 89 years. The CLSI guideline, EP28-A3c, was strictly followed. We also established a reference interval using an indirect approach for comparative purposes, using results from 8466 unselected primary care patients.ResultsThe reference interval for calculated globulin established using direct sampling techniques was 23 g/L (90% CI 22-24 g/L) - 35 g/L (90% CI 34-36 g/L). The reference interval established using indirect sampling techniques in a much larger unselected reference group was 22 g/L (90% CI 22-22 g/L) - 37 g/L (90% CI 37-37 g/L).ConclusionsWe have established a reference interval for calculated globulin, specific to the Roche total protein and BCG albumin methods. This will be a useful tool for other laboratories which use the Roche BCG albumin method, allowing adoption of this reference interval with a simple transference study. The range was a little broader but not materially altered (medians exactly the same) when strict exclusion criteria were removed and also when using a data mining approach, which resulted in a 27-fold larger reference group.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"105-113"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy and implications of minimum retesting intervals for HbA1c. HbA1c最小复检间隔的有效性和意义。

IF 1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-03-01 Epub Date: 2025-08-12 DOI: 10.1177/00045632251367278

Ibrahim Hersi, Rachel L Griffiths, Rousseau Gama, Tejas Kalaria

引用次数: 0

Bilirubin interference on a Roche creatinine enzymatic method. 胆红素对罗氏肌酐酶法的干扰。

IF 1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-03-01 Epub Date: 2025-08-12 DOI: 10.1177/00045632251367271

Stuart Bennett, Angela Woods

引用次数: 0

Blood hydrogen ion concentration: What purpose is served by expressing it as the inverse logarithm (pH)? 血氢离子浓度：用反对数（pH）表示是什么目的？

IF 1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-03-01 Epub Date: 2025-10-17 DOI: 10.1177/00045632251388544

Michael J Murphy

引用次数: 0

A method comparison of the Roche intact PTH method versus the Roche whole PTH (1-84) method: Examining the differences based on eGFR. 罗氏完整甲状旁腺激素法与罗氏全甲状旁腺激素（1-84）法的比较：基于eGFR检测差异

IF 1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-03-01 Epub Date: 2025-06-23 DOI: 10.1177/00045632251356826

Byrne E, Twomey Pj, Crowley Rk, McKenna Mj, Kilbane M

{"title":"A method comparison of the Roche intact PTH method versus the Roche whole PTH (1-84) method: Examining the differences based on eGFR.","authors":"Byrne E, Twomey Pj, Crowley Rk, McKenna Mj, Kilbane M","doi":"10.1177/00045632251356826","DOIUrl":"10.1177/00045632251356826","url":null,"abstract":"AimThird-generation whole PTH (1-84) parathyroid hormone (PTH) assays do not recognize the PTH 7-84 fragment whereas second-generation (intact) assays detect both 1-84 and 7-84 PTH fragments. This study aimed to compare the second-generation Roche intact PTH method with the third-generation Roche whole PTH (1-84) method, examining differences based on estimated glomerular filtration rate (eGFR).MethodsThe intact PTH method and whole PTH (1-84) method were compared using 100 serum samples selected across eGFR quintiles for chronic kidney disease (CKD) stages 1-5 in accordance with Kidney Disease: Improving Global Outcomes (KDIGO).ResultsMethod comparison based on eGFR showed that differences between both PTH methods were not significant at eGFR >60 mL/min/1.73 m2. There was a statistically significant difference at eGFR <60 mL/min/1.73 m2. The whole PTH (1-84) method produced lower results as eGFR decreased: CKD Stage 3 (mean difference: -21%; 95% confidence interval: -16 to -26%) to CKD Stage 5 (mean difference: -46%; 95% confidence interval: -40 to -52%).ConclusionsDifferences observed between the two assays may be due to second-generation PTH assays overestimating PTH concentration by measuring both 1-84 PTH and C-terminal fragments, notably PTH (7-84) in patients with significant renal impairment. The whole PTH (1-84) assay may be used to monitor metabolic bone disease risk. Initial dual reporting of PTH by both methods is recommended for eGFR <60 mL/min to educate users due to the difference in results.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"98-104"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kidney stone analysis may miss diagnoses as demonstrated by a case of adenine phosphoribosyl transferase deficiency. 肾结石分析可能漏诊，如一例腺嘌呤磷酸核糖基转移酶缺乏。

IF 1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-03-01 Epub Date: 2025-08-14 DOI: 10.1177/00045632251367232

Roger Bramley, Daniel Herrera, Robert Barski, Michael Henderson

引用次数: 0

Validation of the Japan Society of Clinical Chemistry (JSCC) recommended reference measurement procedure for glycated albumin determination. 验证日本临床化学学会推荐（JSCC）糖化白蛋白测定参考测量程序。

IF 1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-03-01 Epub Date: 2025-07-29 DOI: 10.1177/00045632251367235

Shu Meguro, Yusuke Iwasaki, Junpei Ueda, Mikiko Okahashi, Yukio Kume, Takuji Kohzuma, Eri Shimizu, Hiroko Takeda, Satoshi Hirayama, Emiko Hosoba, Terumichi Nakagawa, Asako Sato

{"title":"Validation of the Japan Society of Clinical Chemistry (JSCC) recommended reference measurement procedure for glycated albumin determination.","authors":"Shu Meguro, Yusuke Iwasaki, Junpei Ueda, Mikiko Okahashi, Yukio Kume, Takuji Kohzuma, Eri Shimizu, Hiroko Takeda, Satoshi Hirayama, Emiko Hosoba, Terumichi Nakagawa, Asako Sato","doi":"10.1177/00045632251367235","DOIUrl":"10.1177/00045632251367235","url":null,"abstract":"BackgroundTo clarify the fundamental characteristics of the Japan Society of Clinical Chemistry reference measurement procedure (JSCC RMP) for glycated albumin (GA), an additional performance study was performed and the correlation between the HPLC method and JSCC RMP was re-evaluated.MethodsRepeatability, detection limit, addition recovery, uncertainty of measurement, inter-laboratory comparison, correlation between JSCC RMP and HPLC method were evaluated.ResultsThe coefficient of variation (CV) of the total repeatability for the nine pretreated samples including isotope dilution and hydrolysis, defining that the averages of each of nine MS measurement samples are independent measurement vials, was 1.0%(n = 9). The limit of detection and Quantification of the GA values were 7.4 and 29.5 mmol/mol, respectively. Addition recovery rates were 99.6%-100.4%. The strong correlation (r = 0.999) of measured six serum samples between two laboratories was observed. Certified values and expanded uncertainties for JCCRM 611-2 (M, H, HH) using the JSCC RMP were as follows: JCCRM 611-2M: 232 and 9 mmol/mol, JCCRM611-2H: 359 and 14 mmol/mol, JCCRM611-2HH: 556 and 22 mmol/mol, respectively. The regression equation obtained using the Passing-Bablok method was GA (%)HPLC = 0.0523 × GA (mmol/mol) JSCC RMP + 1.315.ConclusionThe basic performance of the JSCC reference procedure for GA measurement was good, and similar results were obtained at other facilities, so it was considered to be robust and suitable as a reference method for GA measurement. Additionally, an equation was established to convert JSCC RMP (mmol/mol) values to the HPLC% values used in clinical practice.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"123-131"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of first-trimester maternal serum proprotein convertase subtilisin/kexin type 9 (PCSK9) levels as a potential non-invasive biomarker for fetal aneuploidy and NTDs. 妊娠早期孕妇血清枯草素/酮素9型（PCSK9）蛋白转化酶水平作为胎儿非整倍体和NTDs潜在的非侵入性生物标志物的分析

IF 1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-03-01 Epub Date: 2025-07-29 DOI: 10.1177/00045632251367280

Karthick E, Sathya Selvarajan, Dhivya Senthil Kumar, Sowmya K, Sridharan K S

{"title":"Analysis of first-trimester maternal serum proprotein convertase subtilisin/kexin type 9 (PCSK9) levels as a potential non-invasive biomarker for fetal aneuploidy and NTDs.","authors":"Karthick E, Sathya Selvarajan, Dhivya Senthil Kumar, Sowmya K, Sridharan K S","doi":"10.1177/00045632251367280","DOIUrl":"10.1177/00045632251367280","url":null,"abstract":"ObjectivesChromosomal abnormalities and congenital anomalies have huge impact on infant mortality and morbidity. The combined incidence affects approximately 1 per 1000 pregnancies. Current diagnostic modalities include ultrasonography and biomarkers like Beta hCG, PAPP-A and Alpha fetoprotein which have limitations due to their varied sensitivity and specificity in aneuploidy and NTD detection. PCSK9, a proprotein converters appears to have an escalating role in neurogenesis, neuronal differentiation and neurological diseases apart from its role in lipid metabolism. This study estimates serum PCSK9 levels in pregnant women with normal gestation and those with high risk for Aneuploidy and NTDs.MethodsThis prospective case control study included 40 pregnant women with high risk of aneuploidy and NTDs (cases) diagnosed by prenatal screening with ultrasonography findings, Beta HCG, PAPP-A and 40 pregnant women with a healthy singleton pregnancy (controls). Statistical analyses were performed in SPSS software version 16. Fetal and maternal characteristics, serum Beta HCG, PAPP-A, PCSK-9 and aneuploidy risk scores were compared between two groups.ResultsThis study observed significant difference in Beta HCG, PAPP-A and PCSK9 levels between the groups (P < .05). The PCSK9 levels were lower in cases [82.95 (70.41-90.74)] than control group [123.84 (102.515-152.70)]. PCSK9 levels <96.8 ng/mL had an 85% sensitivity and specificity. Further PCSK9 correlated with Trisomy 21 risk score and Beta HCG (P < .05).ConclusionsMaternal serum PCSK9 is decreased in high-risk pregnancy during first trimester. With 85% sensitivity, the marker could be a reliable screening tool during prenatal screening which needs further validation.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"114-122"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-life evaluation of an alert system to detect the risk of unreported dyskaelemia in haemolysed blood samples from a hospital emergency department. 在医院急诊科的溶血样本中检测未报告的血钾异常风险的警报系统的现实评估。

IF 1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-03-01 Epub Date: 2025-08-14 DOI: 10.1177/00045632251372764

Valery Brunel, Julie Fettig, Luc Marie Joly, Guillaume Feugray, François Fraissinet, Hélène Girot

{"title":"Real-life evaluation of an alert system to detect the risk of unreported dyskaelemia in haemolysed blood samples from a hospital emergency department.","authors":"Valery Brunel, Julie Fettig, Luc Marie Joly, Guillaume Feugray, François Fraissinet, Hélène Girot","doi":"10.1177/00045632251372764","DOIUrl":"10.1177/00045632251372764","url":null,"abstract":"BackgroundIn vitro haemolysis is a recurrent problem in emergency department samples. Potassium is one of the most critical tests and sensitive to increasing levels of in vitro haemolysis. Haemolysis results in a large number of invalidated potassium test results.MethodsWe set up an alert system to detect the risk of dyskalaemia based on potassium value and haemolysis index (HI). We retrospectively evaluated the effectiveness of the alert system for haemolysed blood samples from the emergency department.Results54 605 samples were included. Women more frequently had a sample with a high HI value, resulting in the invalidation of a potassium test result (3.22% vs 2.35%, P < 0.001). In the case of haemolysed samples, the frequency of alerts for hyperkalemia risk was similar in women and men. Hypokalaemia was significantly more frequent in women than in men (12.71% vs 9.38%, P < 0.001). Among the haemolyzed samples, no hypokalaemia risk alerts were observed and 42 patients had a hyperkalaemia risk alert. For these patients, the potassium value of a second sample was significantly higher in patients with an alert on the first sample (4.85 mmol/L vs 4.0 mmol/L, P < 0.001), with a higher rate of hyperkalaemia (57.14% vs 4.76%, P < 0.001) and critical hyperkalaemia (17.86% vs 0.3%, P < 0.001). Negative predictive value and positive predictive value to detect hyperkalaemia were 90.4% [89.6-91] and 41.3% [25.5%-59.1%].ConclusionsOur alert system was effective to detect the risk of hyperkalemia, and could help clinicians to better target patients requiring repeat sampling for potassium.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"159-166"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0