Annals of Clinical Biochemistry最新文献_第10页

Artificially raised creatinine concentrations due to analytical interference for samples contaminated with total parenteral nutrition fluid. 受全胃肠外营养液污染的样本因分析干扰而导致肌酐浓度人为升高。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-01-01 Epub Date: 2023-07-11 DOI: 10.1177/00045632231186058

Jinny Jeffery, Helen Millar, Rachel Marrington, Finlay MacKenzie, Roanna George

{"title":"Artificially raised creatinine concentrations due to analytical interference for samples contaminated with total parenteral nutrition fluid.","authors":"Jinny Jeffery, Helen Millar, Rachel Marrington, Finlay MacKenzie, Roanna George","doi":"10.1177/00045632231186058","DOIUrl":"10.1177/00045632231186058","url":null,"abstract":"Background: A sample received in the laboratory from a patient receiving total parenteral nutrition (TPN) indicated that the patient may have renal dysfunction, but the results were not considered to be reliable enough to report. Investigations using a reference method for measurement of creatinine confirmed positive interference in the creatinine assay and distribution of samples via an External Quality Assessment (EQA) Scheme showed that this positive interference was method dependent.Methods: Residual TPN fluid (Nutriflex Lipid Special) left in the bag after the patient had completed the infusion was collected and added to a patient serum pool in increasing amounts and distributed to different laboratories for analysis of creatinine and glucose through an EQA Scheme.Results: Positive interference in a number of different creatinine assays was identified as a result of a component in the TPN fluid. Positive interference from high concentrations of glucose has been demonstrated to be a cause for falsely high results in Jaffe creatinine assays.Conclusions: The concern would be that a sample contaminated with TPN fluid would have both abnormal electrolytes and creatinine concentrations and give the impression that the patient was in renal failure due to analytical interference in the creatinine assay and laboratory staff need to be aware of this problem.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"32-38"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9767174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sample viscosity as a source of analytical error: The role of protein structure and mass concentration. 样品粘度是分析误差的来源：蛋白质结构和质量浓度的作用。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-01-01 Epub Date: 2023-09-29 DOI: 10.1177/00045632231205106

Charlton Agius

引用次数: 0

Effect of multiple loading FIT sample collection devices on faecal haemoglobin concentration. 多重装载FIT样本采集装置对粪便血红蛋白浓度的影响。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-01-01 Epub Date: 2023-10-06 DOI: 10.1177/00045632231183850

Cerin John, Carolyn Piggott, Sally C Benton

{"title":"Effect of multiple loading FIT sample collection devices on faecal haemoglobin concentration.","authors":"Cerin John, Carolyn Piggott, Sally C Benton","doi":"10.1177/00045632231183850","DOIUrl":"10.1177/00045632231183850","url":null,"abstract":"Background: Faecal immunochemical tests (FIT) are routinely used in colorectal cancer (CRC) screening programmes around the world. More recently, quantitative FIT has been recommended to help triage patients presenting to primary care with symptoms suggestive of CRC. Participants collect faecal samples using sampling probes which are inserted into sample collection devices (SCDs) containing preservative buffer. The SCDs have an internal collar designed to remove excess sample. The aim of this study was to investigate the impact of multiple loading on faecal haemoglobin concentration (f-Hb) using SCDs of four FIT systems.Methods: Pools of f-Hb negative samples were spiked with blood, homogenised and loaded into SCDs 1, 3 and 5 times, with insertion of the sampling probes into the SCDs with and without mixing between loads. The f-Hb was measured using the relevant FIT system. The percentage change in f-Hb for multiple loads was compared with a single load for each system for the mixed and unmixed groups.Results: The p values show a significant difference (p < 0.05) in the mass and f-Hb for the mixed and unmixed group, for 1-3 and 1-5 loads for all systems. The median percentage change in f-Hb for the mixed is higher than the unmixed group.Conclusion: This study showed that multiple loading does significantly increase the f-Hb in the SCDs.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"70-73"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9593211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The LEAP checklist for laboratory evaluation and analytical performance characteristics reporting of clinical measurement procedures. LEAP检查表，用于临床测量程序的实验室评估和分析性能特征报告。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-01-01 Epub Date: 2023-10-15 DOI: 10.1177/00045632231206029

Tze Ping Loh, Brian R Cooke, Thi Chi Mai Tran, Corey Markus, Rosita Zakaria, Chung Shun Ho, Elvar Theodorsson, Ronda F Greaves

{"title":"The LEAP checklist for laboratory evaluation and analytical performance characteristics reporting of clinical measurement procedures.","authors":"Tze Ping Loh, Brian R Cooke, Thi Chi Mai Tran, Corey Markus, Rosita Zakaria, Chung Shun Ho, Elvar Theodorsson, Ronda F Greaves","doi":"10.1177/00045632231206029","DOIUrl":"10.1177/00045632231206029","url":null,"abstract":"Reporting a measurement procedure and its analytical performance following method evaluation in a peer-reviewed journal is an important means for clinical laboratory practitioners to share their findings. It also represents an important source of evidence base to help others make informed decisions about their practice. At present, there are significant variations in the information reported in laboratory medicine journal publications describing the analytical performance of measurement procedures. These variations also challenge authors, readers, reviewers and editors in deciding the quality of a submitted manuscript. The International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Method Evaluation Protocols (IFCC WG-MEP) developed a checklist and recommends its adoption to enable a consistent approach to reporting method evaluation and analytical performance characteristics of measurement procedures in laboratory medicine journals. It is envisioned that the LEAP checklist will improve the standardisation of journal publications describing method evaluation and analytical performance characteristics, improving the quality of the evidence base that is relied upon by practitioners.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"3-7"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of in vitro stability of routine haematinics tests using EFLM standards and the CRESS checklist. 用EFLM标准和CRESS检查表测定常规血液学试验的体外稳定性。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2023-11-01 Epub Date: 2023-05-25 DOI: 10.1177/00045632231177247

Robert D Williams, Christopher J Duff, Alexandra Yates

{"title":"Determination of in vitro stability of routine haematinics tests using EFLM standards and the CRESS checklist.","authors":"Robert D Williams, Christopher J Duff, Alexandra Yates","doi":"10.1177/00045632231177247","DOIUrl":"10.1177/00045632231177247","url":null,"abstract":"Background: Laboratories should be aware of the stability of the analytes they are testing in order to avoid incorrect reporting and patient management. Stability studies are difficult to interpret and reproduce, with little guidance on how to determine appropriate clinical cut off values. Here we describe a standardised approach to determining stability for routine haematinics tests using published EFLM guidelines.Methods: The haematinics panel at UHNM contains vitamin B12, folate, ferritin, iron and transferrin. Blood tubes included were serum separator tubes, gel-free serum and lithium-heparin plasma. Conditions tested were room temperature, 2-8°C and -20°C. For each condition and tube, three samples were analysed in duplicate at 0, 24, 48, 72, 96 and 120 h using the Siemens Atellica platform.Results: The percentage difference was calculated for each respective blood tube and storage condition, in addition to individual analyte maximum permissible instability scores. The majority of analytes for all blood tubes were stable for 5 days or more when stored at 4-8°C and -20°C. Ferritin (excluding gel-free), iron and transferrin further showed stability >5 days when stored at room temperature. However, vitamin B12 and folate demonstrated poor stability data for all tube types tested.Conclusions: Here we describe a stability study for the haematinics panel on the Siemens Atellica platform using the standardised EFLM Checklist for Reporting Stability Studies (CRESS). The checklist was used in order to promote a standardised and transferable scientific approach to what has previously been lacking in the literature when performing stability experiments.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"367-373"},"PeriodicalIF":2.2,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9507923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

National recommendations to standardise acute kidney injury detection and alerting. 国家对急性肾损伤检测和报警标准化的建议。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2023-11-01 Epub Date: 2023-06-14 DOI: 10.1177/00045632231180403

Rachel Marrington, Anna L Barton, Alexandra Yates, William McKane, Nicholas M Selby, Jonathan S Murray, James F Medcalf, Finlay MacKenzie, Martin Myers

{"title":"National recommendations to standardise acute kidney injury detection and alerting.","authors":"Rachel Marrington, Anna L Barton, Alexandra Yates, William McKane, Nicholas M Selby, Jonathan S Murray, James F Medcalf, Finlay MacKenzie, Martin Myers","doi":"10.1177/00045632231180403","DOIUrl":"10.1177/00045632231180403","url":null,"abstract":"Background: National Health Service England issued a Patient Safety Alert in 2014 mandating all acute Trusts in England to implement Acute Kidney Injury (AKI) warning stage results and to do so using a standardised algorithm. In 2021, the Renal and Pathology Getting It Right First Time (GIRFT) teams found significant variation in AKI reporting across the UK. A survey was designed to capture information on the entire AKI detection and alerting process to investigate the potential sources of this unwarranted variation.Methods: In August 2021, an online survey consisting of 54 questions was made available to all UK laboratories. The questions covered creatinine assays, laboratory information management systems (LIMS), the AKI algorithm and AKI reporting.Results: We received 101 responses from laboratories. Data were reviewed for England only - 91 laboratories. Findings included that 72% used enzymatic creatinine. In addition, 7 manufacturer-analytical platforms, 15 different LIMS and a wide range of creatinine reference ranges were in use. In 68% of laboratories, the AKI algorithm was installed by the LIMS provider. Marked variation was found in the minimum age of AKI reporting with only 18% starting at the recommended 1 month/28-days. Some 89% phoned all new AKI2s and AKI3s, as per AKI guidance while 76% provided comments/hyperlinks in reports.Conclusions: The national survey has identified laboratory practices that potentially contribute to unwarranted variation in the reporting of AKI in the England. This has formed the basis for improvement work to remedy the situation, including national recommendations, included within this article.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"406-416"},"PeriodicalIF":2.2,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10003330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interference by paraproteins is an infrequent finding in nine Roche c702 assays. 副蛋白的干扰在9个罗氏c702试验中是一个罕见的发现。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2023-11-01 Epub Date: 2023-08-10 DOI: 10.1177/00045632231194435

Alison M Jones, Stuart J Wilkinson

引用次数: 0

A further monoclonal antibody-based immunoturbidimetry option for measuring faecal elastase on the Optilite analyser. 另一种基于单克隆抗体的免疫比浊法选择，用于在Optilite分析仪上测量粪便弹性蛋白酶。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2023-11-01 Epub Date: 2023-07-20 DOI: 10.1177/00045632231190516

Alessia Capoferri, Ilenia Infusino, Mauro Panteghini

引用次数: 0

Vitamin B12 reference intervals on Beckman, Roche and Siemens analytical platforms. 贝克曼，罗氏和西门子分析平台的维生素B12参考区间。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2023-11-01 Epub Date: 2023-09-12 DOI: 10.1177/00045632231202211

Nuthar Jassam, Thomas Bancroft, Ahai Luvai, Wycliffe Mbagaya, Julian H Barth

{"title":"Vitamin B12 reference intervals on Beckman, Roche and Siemens analytical platforms.","authors":"Nuthar Jassam, Thomas Bancroft, Ahai Luvai, Wycliffe Mbagaya, Julian H Barth","doi":"10.1177/00045632231202211","DOIUrl":"10.1177/00045632231202211","url":null,"abstract":"Background: Vitamin B12 status is assessed primarily by measuring total serum B12 using competitive binding methods. The lack of availability of a standard material and high-level reference measurement procedure affect the trueness of B12 results; this results in variation between methods. This study aimed to determine the reference intervals for vitamin B12 on three routine analytical platforms.Method: A prospective reference population of healthy individuals was recruited according to the IFCC CRIDL criteria. Vitamin B12 samples were measured on Roche, Beckman and Siemens analytical platforms.Results: In total, 300 adult subjects were recruited; the central 95th centile values for B12 for Roche (190-678 ng/mL) and Siemens (181-562 ng/mL) analytical platforms were in a close agreement. Beckman DXi, however, showed a significantly lower reference limit (110-562 ng/mL). All reference intervals are in keeping with previously published data but some are not in agreement with manufacturer provided reference interval.Conclusion: As the quality of the reference intervals plays a significant role in clinical outcome, it is of great importance that laboratories use a method-specific reference interval and if possible, locally derived reference intervals until further method standardization occurs.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"417-422"},"PeriodicalIF":2.2,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10275007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dataset dependency of low-density lipoprotein-cholesterol estimation by machine learning. 机器学习估计低密度脂蛋白-胆固醇的数据集依赖性。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2023-11-01 Epub Date: 2023-06-05 DOI: 10.1177/00045632231180408

Ishida Hidekazu, Hiroki Nagasawa, Yasuko Yamamoto, Hiroki Doi, Midori Saito, Yuya Ishihara, Takashi Fujita, Mariko Ishida, Yohei Kato, Ryosuke Kikuchi, Hidetoshi Matsunami, Masao Takemura, Hiroyasu Ito, Kuniaki Saito

{"title":"Dataset dependency of low-density lipoprotein-cholesterol estimation by machine learning.","authors":"Ishida Hidekazu, Hiroki Nagasawa, Yasuko Yamamoto, Hiroki Doi, Midori Saito, Yuya Ishihara, Takashi Fujita, Mariko Ishida, Yohei Kato, Ryosuke Kikuchi, Hidetoshi Matsunami, Masao Takemura, Hiroyasu Ito, Kuniaki Saito","doi":"10.1177/00045632231180408","DOIUrl":"10.1177/00045632231180408","url":null,"abstract":"Objectives: We evaluated the applicability of a machine learning-based low-density lipoprotein-cholesterol (LDL-C) estimation method and the influence of the characteristics of the training datasets.Methods: Three training datasets were chosen from training datasets: health check-up participants at the Resource Center for Health Science (N = 2664), clinical patients at Gifu University Hospital (N = 7409), and clinical patients at Fujita Health University Hospital (N = 14,842). Nine different machine learning models were constructed through hyperparameter tuning and 10-fold cross-validation. Another test dataset of another 3711 clinical patients at Fujita Health University Hospital was selected as the test set used for comparing and validating the model against the Friedewald formula and the Martin method.Results: The coefficients of determination of the models trained on the health check-up dataset produced coefficients of determination that were equal to or inferior to those of the Martin method. In contrast, the coefficients of determination of several models trained on clinical patients exceeded those of the Martin method. The means of the differences and the convergences to the direct method were higher for the models trained on the clinical patients' dataset than for those trained on the health check-up participants' dataset. The models trained on the latter dataset tended to overestimate the 2019 ESC/EAS Guideline for LDL-cholesterol classification.Conclusion: Although machine learning models provide valuable method for LDL-C estimates, they should be trained on datasets with matched characteristics. The versatility of machine learning methods is another important consideration.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"396-405"},"PeriodicalIF":2.2,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9944734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0