Annals of Clinical Biochemistry最新文献

{"title":"EDTA plasma in glass and polyethylene terephthalate tubes is suitable for measurement of B-type natriuretic peptide on the Mindray CL-6000i.","authors":"Wei Zhao,&nbsp;Gang Feng,&nbsp;Jun Wan,&nbsp;Xing Wang,&nbsp;Jiping Feng,&nbsp;Wanwan Zhang,&nbsp;Yalan Yu,&nbsp;Rui Pu,&nbsp;Yong Du,&nbsp;Yongtong Cao","doi":"10.1177/00045632231162289","DOIUrl":"10.1177/00045632231162289","url":null,"abstract":"<p><strong>Aims: </strong>To explore differences in B-type natriuretic peptide (BNP) concentration and stability and evaluate BNP accuracy in different collection tubes.</p><p><strong>Methods: </strong>BNP concentrations in heparin/glass, EDTA/glass, and EDTA/polyethylene terephthalate (PET) tubes were measured on the Mindray CL-6000i at 0.5, 1, 2, and 4 h after collection. Differences were evaluated using Wilcoxon's paired tests and Bland-Altman plots. BNP stability and measurement accuracies were estimated using Kruskal-Wallis H tests and recovery tests.</p><p><strong>Results: </strong>BNP concentrations in EDTA/glass tubes were 31.4% higher than those in heparin/glass tubes and 3.04% lower than those in EDTA/PET tubes. BNP stability significantly decreased in the heparin/glass tube. BNP remained stable in EDTA/glass and EDTA/PET tubes at room temperature for 4 h. BNP recovery rates in heparin/glass, EDTA/glass, and EDTA/PET tubes were 77.46, 86.04, and 88.23%, respectively.</p><p><strong>Conclusions: </strong>Plasma in EDTA/glass and EDTA/PET tubes is suitable for BNP measurement on the Mindray CL-6000i.</p>","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"306-312"},"PeriodicalIF":2.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9099121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of serum M-protein in acetonitrile precipitates by MALDI-TOF mass spectrometry: A novel, low-cost methodology.","authors":"Nikita Mehra,&nbsp;Gopal Gopisetty,&nbsp;Jayavelu Subramani,&nbsp;Sariga Dhanasekar,&nbsp;Arivazhagan Rajamanickam,&nbsp;Jayachandran Perumal Kalaiyarasi,&nbsp;Parathan Karunakaran,&nbsp;Krishnarathinam Kannan,&nbsp;Swaminathan Rajaraman,&nbsp;Thangarajan Rajkumar","doi":"10.1177/00045632231174144","DOIUrl":"10.1177/00045632231174144","url":null,"abstract":"<p><strong>Background: </strong>Several studies have demonstrated the analytical sensitivity of MALDI-TOF mass spectrometry (MALDI-TOF MS) by immunoenrichment for M-protein analysis. We report the results of a novel, low-cost, reagent-based extraction process using acetonitrile (ACN) precipitation to enrich for κ and λ light chains which can be analysed by MALDI-TOF MS.</p><p><strong>Methods: </strong>Institutional Ethics committee approval was obtained. Serum samples from patients with monoclonal gammopathy of undetermined significance (MGUS), multiple myeloma (MM), plasmacytoma, AL amyloidosis and Waldenström macroglobulinemia (WM) underwent ACN precipitation. The images obtained were overlaid on apparently healthy donor serum samples to confirm the presence of M-protein. A sample was considered positive for M-protein if there was a sharp or broad peak within the κ or λ mass/charge (<i>m/z)</i> range: <i>m/z-</i> [M + 2H]²⁺: 11,550-12,300 Da and λ <i>m/z</i>- [M + 2H]²⁺: 11,100-11,500 Da. Images were acquired at a <i>m/z</i> range of 10,000-29,000 Da. Corresponding serum protein electrophoresis (SPEP), serum immunofixation electrophoresis (IFE) and serum free light chain (sFLC) assay by nephelometry were performed for all the samples.</p><p><strong>Results: </strong>Two-hundred-and-two serum samples were included in the study: MM- 184 (91%); AL amyloidosis- 2 (1%); plasmacytoma- 8 (4%); MGUS- 6 (3%) and WM- 2 (1%). All the SPEP positive samples were identified by MALDI-TOF MS. Out of 179 samples positive for M-protein by IFE, MALDI-TOF MS was positive in 176 samples (98%). Compared to IFE, the sensitivity and specificity of M-protein identification by MALDI-TOF MS were 98.3% and 52.2%, respectively.</p><p><strong>Conclusions: </strong>This study demonstrates the feasibility of qualitatively identifying M-protein without the need for antibody-based immunoenrichment, making the technique cost-effective.</p>","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"339-348"},"PeriodicalIF":2.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9472285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepcidin analysis in pneumonia: Comparison of immunoassay and LC-MS/MS.","authors":"Kjersti Oppen,&nbsp;Cato Brede,&nbsp;Øyvind Skadberg,&nbsp;Trude Steinsvik,&nbsp;Jan Cato Holter,&nbsp;Annika E Michelsen,&nbsp;Lars Heggelund","doi":"10.1177/00045632231159529","DOIUrl":"10.1177/00045632231159529","url":null,"abstract":"<p><strong>Background: </strong>The iron-regulatory hormone hepcidin is a promising biomarker to differentiate anaemia of inflammation from iron deficiency. Plasma hepcidin concentrations increase substantially during inflammation, and the amount of smaller, non-biologically active isoforms of hepcidin increase in inflammatory conditions. These smaller isoforms are measured in some, but not all analytical methods. Thus, we evaluated the comparability of two analytical methods with different isoform selectivity during and after acute-phase pneumonia as a highly inflammatory model disease.</p><p><strong>Methods: </strong>Blood samples from a cohort of 267 hospitalized community-acquired pneumonia patients collected at admission and a 6-week follow-up were analysed. Hepcidin was measured in plasma by an immunoassay, which recognizes all hepcidin isoforms, and a liquid chromatography tandem mass spectrometry (LC-MS/MS), which selectively measures the bioactive hepcidin-25. Additionally, a subset of serum samples was analysed by LC-MS/MS.</p><p><strong>Results: </strong>Hepcidin measurements by immunoassay were higher compared with LC-MS/MS. The relative mean difference of hepcidin plasma concentrations between the two analytical methods was larger in admission samples than in follow-up samples (admission samples <200 ng/mL: 37%, admission samples >200 ng/mL: 78%, follow-up samples >10 ng/mL: 22%). During acute-phase pneumonia, serum concentrations were on average 22% lower than plasma concentrations when measured by LC-MS/MS.</p><p><strong>Conclusions: </strong>Immunoassay measured higher hepcidin concentrations compared with LC-MS/MS, with more pronounced differences in high-concentration samples during acute-phase pneumonia. These findings should be considered in local method validations and in future harmonization and standardization optimization of hepcidin measurements.</p>","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"298-305"},"PeriodicalIF":2.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ef/a9/10.1177_00045632231159529.PMC10552342.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10831699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First case of very late-onset FHL2 in Spain with two variants in the PRF1 gene.","authors":"Paula Sienes Bailo,&nbsp;Nuria Goñi Ros,&nbsp;Bárbara Menéndez Jándula,&nbsp;Ramiro Álvarez Alegret,&nbsp;Eduardo González Gómez,&nbsp;Ricardo González Tarancón,&nbsp;Silvia Izquierdo Álvarez","doi":"10.1177/00045632231186076","DOIUrl":"10.1177/00045632231186076","url":null,"abstract":"<p><p>Hemophagocytic lymphohistiocytosis (HLH) is a rare but fatal disorder characterized by the proliferation and infiltration of macrophages and hyperactivated T lymphocytes that escape from the physiological control pathways and favour the existence of an environment of excessive inflammation and tissue destruction. HLH has been classified into two types: a primary or familial autosomal recessive form, caused by mutations in genes encoding proteins involved in the granule-dependent cytotoxic pathway (familial hemophagocytic lymphohistiocytosis [FHL] types 1-5); and other secondary or acquired form, generally associated with infections, malignancy, autoimmune diseases, metabolic disorders or primary immunodeficiencies. Since the first familial hemophagocytic lymphohistiocytosis-2 (FHL2) causative mutation in the <i>PRF1</i> gene was described in 1999, more than 200 mutations have been identified to date. Here, we report the first case of very late-onset FHL2 in a Spanish 72-year-old female with splenomegaly, hypertriglyceridemia, hypofibrinogenemia, pancytopenia and marrow hemophagocytosis harbouring in heterozygosity two <i>PRF1</i> variants proposed as causative in this study. The heterozygous mutation c.445G>A (p.Gly149Ser) identified in the exon 2 results in a missense mutation previously described as a probable pathogenic variant associated with the development of FHL2. Affecting the same exon, c.272C>T (p.Ala91Val) is the most prevalent variant of this gene. Although it was initially classified as benign, recent studies support its potential pathogenic role, considering it a variant of uncertain significance associated with a risk of developing FHL2. The genetic confirmation of FHL made possible an adequate counselling to the patient and direct relatives and provided important information for her control and follow-up.</p>","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"356-364"},"PeriodicalIF":2.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9689083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation of eGFR reporting and CKD equations used in the United Kingdom.","authors":"Rachel Marrington,&nbsp;Finlay MacKenzie","doi":"10.1177/00045632231173233","DOIUrl":"10.1177/00045632231173233","url":null,"abstract":"<p><strong>Background: </strong>UK Clinical laboratories have been routinely reporting an estimated glomerular filtration rate (eGFR) based on creatinine measurements using an eGFR equation since the early 2000s. Though there have been recommendations to use enzymatic based creatinine assays, and a recommendation of which equation to use, there still remains a high degree of variation in calculated eGFR results.</p><p><strong>Methods: </strong>Data from the UK NEQAS for Acute and Chronic Kidney Disease Scheme have been reviewed to look at the CKD equations that are currently in use in the UK and the impact on eGFR results reported. The UK NEQAS for Acute and Chronic Kidney Disease has over 400 participants measuring creatinine across all major clinical biochemistry platforms.</p><p><strong>Results: </strong>An audit of EQA registration against results returned showed that in February 2022 at most 44% of registered participants were correctly reporting the 2009 CKD-EPI equation. At higher creatinine concentrations (which give rise to lower eGFR results), the spread of eGFRs is tight and there is little difference between results from different method principles. However, at lower creatinine concentrations, where it is known that there is more variation in creatinine depending on method choice, both method principle and eGFR equation choice can influence calculated eGFR. In some cases, this can impact CKD Stage classification.</p><p><strong>Conclusions: </strong>CKD is a serious public health issue that requires accurate assessment of eGFR. Laboratories should be in constant dialogue with their renal teams about their creatinine assay performance and impact on eGFR reporting across their service.</p>","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"328-338"},"PeriodicalIF":2.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9760532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UKMedLab2023 conference - A celebration of 70 years of the ACB.","authors":"Sarah Robinson,&nbsp;Kath Hayden","doi":"10.1177/00045632231193581","DOIUrl":"10.1177/00045632231193581","url":null,"abstract":"UKMedLab23, hosted by the Association for Clinical Biochemistry and Laboratory Medicine (ACB), was held at the Royal Armouries, Leeds, between the 10th and 12th June 2023. This year the conference celebrated 70 years of the ACB. With this in mind, and to celebrate the work and contribution of all members, each region was invited to host a parallel session at the National Meeting. All nine ACB regions accepted the invitation with enthusiasm and vigour and planned sessions showcasing the varied specialisms across the UK and Republic of Ireland. There was also a parallel session focused on the current challenges in POCT, on the top of the highly popular interactive case sessions, the Medal Award presentations and the Impact, Foundation and International plenary lectures, a special 70th anniversary President’s address and a number of industry-sponsored workshops. The sun shone and the conversation flowed, with 341 delegates, there was a real buzz about the conference venue as colleagues caught up with peers and discussed the latest advances in the field. Following the success of last year, an ACBMicrobiology training day was again held alongside the Biochemistry training day. The morning was a shared programme on management topics and scenarios and in the afternoon split discipline-specific sessions were held. The training day attracted 34 microbiology delegates and 119 biochemistry delegates, which was an increase for both cohorts from the previous year. It was wonderful to see so many colleagues in training coming together and building their networks. The meeting was opened by Bernie Croal, before the programme commenced with the International Lecture. This was presented byMaria Fitzgibbon on lessons in Chemistry, with a focus on the use and evidence for biomarkers of inflammation in cardiovascular and other diseases. This was a great example of how research can be fostered and flourished in a supportive environment with a group of collaborating scientists. A number of high-quality submissions were received for the Impact Award, and this year’s worthy winner was Tim MacDonald for the work that he and his team completed on the development of a national home finger-prick blood laboratory testing service for clinicians of Paediatric Highly Specialist Services for Complex Conditions. This inspiring talk gave the audience an idea of what is possible when teams work together to improve patient outcome and the future possibilities of home blood sampling. Following a successful change to the nomination process for the Foundation Award, more nominations were received than ever before, and it was a really difficult decision for the judging panel. This year’s winner was Paul Collinson, who gave a detailed insight into the evolution of cardiac biomarkers from the first ECGs and the use of WBC, ESR and CRP to the measurement of troponin T and I today and the potential uses for the future. He also gave an interesting reminder about the small changes that it is n","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"295-297"},"PeriodicalIF":2.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10167647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Falsely elevated thyroid hormone levels associated with fibrin interference in patients receiving oral anticoagulant therapy.","authors":"Mitsuaki Tokumaru,&nbsp;Kenji Ohba,&nbsp;Yumiko Kashiwabara,&nbsp;Hiroyuki Takase,&nbsp;Chiga Hayashi,&nbsp;Takayuki Iwaki,&nbsp;Yasuhide Suzuki,&nbsp;Akio Matsushita,&nbsp;Shigekazu Sasaki,&nbsp;Takafumi Suda,&nbsp;Masato Maekawa","doi":"10.1177/00045632231159280","DOIUrl":"https://doi.org/10.1177/00045632231159280","url":null,"abstract":"<p><strong>Objective: </strong>Unique clinical courses were observed in two asymptomatic patients receiving warfarin who referred to our hospital because of suspected central hyperthyroidism. We eventually diagnosed these patients with falsely elevated thyroid hormone levels caused by macroscopically invisible fibrin. Although false results caused by fibrin interference in vitro have been identified in various immunoassays, especially in blood samples from patients receiving anticoagulant therapy, no studies on thyroid function testing have been reported. The experience in evaluating these cases prompted us to investigate the independent influence of oral anticoagulants via putative fibrin interference on thyroid function testing.</p><p><strong>Methods: </strong>We retrospectively reviewed known contributing factors that affect thyroid function testing including age, gender, medication history, body mass index, estimated glomerular filtration rate, smoking status, alcohol consumption, and the seasons of hospital visits from participants who presented the Department of Health Checkup between April 2010 and December 2020.</p><p><strong>Results: </strong>A propensity-matched analysis revealed that the median serum free thyroxine levels under oral anticoagulant were significantly higher (17.9 pmol/L, n = 60) than those without anticoagulants (16.0 pmol/L, n = 60; <i>p</i> < 0.001). It was noted that this difference was the largest among contributing factors we analyzed. No significant differences were noted in serum thyroid-stimulating hormone levels.</p><p><strong>Conclusions: </strong>We report two patients receiving warfarin with falsely elevated thyroid hormone levels caused by fibrin interference resembling central hyperthyroidism for the first time. Our retrospective study suggests that the medication status of oral anticoagulants should be considered when evaluating thyroid function tests.</p>","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":"60 4","pages":"249-258"},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9838125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effectiveness of an automated algorithm as a tool for investigating the cause of anaemia in undiagnosed patients from general practitioners.","authors":"Anne Margreet de Jong,&nbsp;Sam Veldhuis,&nbsp;Firmin Candido,&nbsp;Wendy Pj den Elzen,&nbsp;Bauke A de Boer","doi":"10.1177/00045632231160663","DOIUrl":"https://doi.org/10.1177/00045632231160663","url":null,"abstract":"<p><strong>Background: </strong>The Dutch guideline algorithm for the analysis of anaemia in patients of general practitioners (GPs) was programmed in a Clinical Decision Support system (CDS-anaemia) to support the process of diagnosing the cause of anaemia in the laboratory. This study investigates the diagnostic yield of the automated anaemia algorithm compared to that of the manual work up by the GP.</p><p><strong>Methods: </strong>This retrospective population-based study consisted of 2697 people ≥18 years. Anaemia was defined according to the Dutch College of General Practitioners (DCGP) guideline. Causes of anaemia were based on the DCGP guidelines with the corresponding blood tests. The number of blood tests and causes of anaemia were measured in two separate periods in both the (CDS-anaemia) pilot group and a control group in which routine care was provided.</p><p><strong>Results: </strong>Patients from GPs supported by CDS-anaemia had higher chances of having more anaemia-related blood tests being performed. This resulted in finding significantly more causes of anaemia in the pilot group compared to the control group with respect to iron deficiency (resp. 31.3% vs 14.5%), possible iron deficiency (resp. 11.4% vs 2.8%), iron deficiency in acute phase (2.6% vs 0.5%), chronic disease/infection/inflammation (23.5% vs 1.9%), vitamin B12 deficiency (4.5% vs 1.9%), possible vitamin B12 deficiency (16.8% vs 8.7%), folate deficiency (3.3% vs 0.9%) and possible bone marrow disorder (3.8% vs 0.0%); <i>p</i> < 0.05.</p><p><strong>Conclusions: </strong>This study suggests that an automated-algorithm support can effectively aid in the diagnostic work-up of anaemia in primary care to find more causes of anaemia.</p>","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":"60 4","pages":"270-278"},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9831494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of faecal calprotectin using two collection and extraction strategies for the BÜHLMANN CALEX® Cap - possible implications for clinical cut-offs?","authors":"Helen Bruce,&nbsp;Jacqueline Osypiw,&nbsp;Geeta Prajapati-Jha,&nbsp;Shane O'Driscoll,&nbsp;Martin Brealey,&nbsp;Sally C Benton","doi":"10.1177/00045632231159296","DOIUrl":"https://doi.org/10.1177/00045632231159296","url":null,"abstract":"<p><strong>Background: </strong>Faecal calprotectin has been identified as a useful biochemical marker in the differentiation of inflammatory bowel disease and irritable bowel syndrome. Typically, patients send faecal specimens in a pot for manual extraction by the laboratory. During the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS CoV-2) pandemic, the routine laboratory service was temporarily suspended due to the potential increased risk to staff. In this study we investigated the possibility of patients collecting samples directly into the faecal extraction tubes.</p><p><strong>Method: </strong>Patients submitted paired faecal samples for calprotectin analysis using a standard faecal container (current practice) and followed instructions for faecal collection using the BÜHLMANN CALEX® Cap device. Samples were returned to the laboratory immediately after collection. Laboratory staff manually extracted the calprotectin from the faecal samples using the CALEX® Cap prior to analysis of both extracts on the Cobas c702.</p><p><strong>Results: </strong>91 paired faecal samples were included in the study. Clinical correlation was found to be 70% with numerical correlation showing a positive bias for the patient-collected CALEX® Cap sample when compared to the laboratory-extracted faecal sample around the clinical decision points 100-250 μg calprotectin/g faeces.</p><p><strong>Conclusion: </strong>The study shows that collection of a faecal sample using the CALEX® Cap works well and is a good alternative to using standard containers. The correlation gives rise to the possibility that faecal calprotectin is not stable when collected into standard collection containers. Prior to further roll-out of this process, questions surrounding the current cut-offs would need to be addressed.</p>","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":"60 4","pages":"236-242"},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9876958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subjects used to calculate pregnancy-specific reference intervals must exclude those who are overweight or obese.","authors":"William Tormey","doi":"10.1177/00045632231159783","DOIUrl":"https://doi.org/10.1177/00045632231159783","url":null,"abstract":"Samuel Dockree, Brian Shine, Tim James and Manu Vatish Women’s Centre, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, UK Department of Clinical Biochemistry, John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust, Oxford, UK Nuffield Department of Women’s and Reproductive Health, University of Oxford, UK Bill and Melinda Gates Foundation, Seattle, WA, USA","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":"60 4","pages":"291"},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10217944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}