Annals of Clinical Biochemistry最新文献_第10页

Increased PD-1 expression of bone marrow T-cells in acute myeloid leukaemia patients after stem cell transplantation, and its association with overall survival. 干细胞移植后急性髓系白血病患者骨髓t细胞PD-1表达升高及其与总生存率的关系

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-11-21 DOI: 10.1177/00045632231184716

Eunkyoung You, Chan-Jeoung Park, Young-Uk Cho, Seongsoo Jang, Min Young Lee, Hery Kim, Kyung Nam Koh, Ho Joon Im, Eun-Ji Choi, Je-Hwan Lee, Kyoo-Hyung Lee

{"title":"Increased PD-1 expression of bone marrow T-cells in acute myeloid leukaemia patients after stem cell transplantation, and its association with overall survival.","authors":"Eunkyoung You, Chan-Jeoung Park, Young-Uk Cho, Seongsoo Jang, Min Young Lee, Hery Kim, Kyung Nam Koh, Ho Joon Im, Eun-Ji Choi, Je-Hwan Lee, Kyoo-Hyung Lee","doi":"10.1177/00045632231184716","DOIUrl":"10.1177/00045632231184716","url":null,"abstract":"Background: Immune checkpoints are involved in mechanisms by which tumours escape from the host immune system. Our aim was to evaluate acute myeloid leukaemia (AML) patients to determine expression levels of checkpoint molecules according to diagnosis and treatments, and to identify optimal candidates for checkpoint blockade.Methods: Bone marrow (BM) samples were obtained from 279 AML patients at different disease status and from 23 controls. Flow cytometric analyses of PD-1 and PD-L1/PD-L2 expression were performed.Results: Programmed death-1 (PD-1) expression levels on CD8+ T-cells at AML diagnosis were increased compared to controls. PD-L1 and PD-L2 expression levels on leukaemic cells at diagnosis were significantly higher in secondary AML than in de novo AML. PD-1 levels on CD8+ and CD4+ T-cells after allo-SCT were significantly higher than those at diagnosis and after CTx. PD-1 expression on CD8+ T-cells increased in the acute GVHD group than in the non-GVHD group. The overall survival of patients with high PD-1 expression on CD8+ T-cells was significantly shorter than that of patients with low PD-1 expression.Conclusions: In conclusion, patients who underwent allo-SCT exhibited high PD-1 expression, suggesting that allo-SCT increases PD-1 expression on T-cells, and the patients with high PD-1 expression on CD8+ T-cells after allo-SCT showed the poor prognosis. For these patients, PD-1 blockade could be an immunotherapeutic strategy.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"79-89"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9630852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can artificial intelligence replace biochemists? A study comparing interpretation of thyroid function test results by ChatGPT and Google Bard to practising biochemists. 人工智能能取代生物化学家吗？一项将ChatGPT和Google Bard对甲状腺功能测试结果的解释与执业生物化学家进行比较的研究。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-09-20 DOI: 10.1177/00045632231203473

Emma Stevenson, Chelsey Walsh, Luke Hibberd

{"title":"Can artificial intelligence replace biochemists? A study comparing interpretation of thyroid function test results by ChatGPT and Google Bard to practising biochemists.","authors":"Emma Stevenson, Chelsey Walsh, Luke Hibberd","doi":"10.1177/00045632231203473","DOIUrl":"10.1177/00045632231203473","url":null,"abstract":"Background: Public awareness of artificial intelligence (AI) is increasing and this novel technology is being used for a range of everyday tasks and more specialist clinical applications. On a background of increasing waits for GP appointments alongside patient access to laboratory test results through the NHS app, this study aimed to assess the accuracy and safety of two AI tools, ChatGPT and Google Bard, in providing interpretation of thyroid function test results as if posed by laboratory scientists or patients.Methods: Fifteen fictional cases were presented to a team of clinicians and clinical scientists to produce a consensus opinion. The cases were then presented to ChatGPT and Google Bard as though from healthcare providers and from patients. The responses were categorized as correct, partially correct or incorrect compared to consensus opinion and the advice assessed for safety to patients.Results: Of the 15 cases presented, ChatGPT and Google Bard correctly interpreted only 33.3% and 20.0% of cases, respectively. When queries were posed as a patient, 66.7% of ChatGPT responses were safe compared to 60.0% of Google Bard responses. Both AI tools were able to identify primary hypothyroidism and hyperthyroidism but failed to identify subclinical presentations, non-thyroidal illness or secondary hypothyroidism.Conclusions: This study has demonstrated that AI tools do not currently have the capacity to generate consistently correct interpretation and safe advice to patients and should not be used as an alternative to a consultation with a qualified medical professional. Available AI in its current form cannot replace human clinical knowledge in this scenario.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"143-149"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10214754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of finer needles for venipuncture increases in vitro haemolysis despite reducing persistent pain and nerve injury: A retrospective study. 使用更细的针头进行静脉穿刺会增加体外溶血，尽管会减少持续疼痛和神经损伤：一项回顾性研究。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-08-29 DOI: 10.1177/00045632231196045

Hiroshi Umemura, Hiromichi Takahashi, Yoshiaki Fukuda, Hitoshi Soma, Rumiko Aoki, Norihisa Takei, Tomohiro Nakayama

{"title":"Use of finer needles for venipuncture increases in vitro haemolysis despite reducing persistent pain and nerve injury: A retrospective study.","authors":"Hiroshi Umemura, Hiromichi Takahashi, Yoshiaki Fukuda, Hitoshi Soma, Rumiko Aoki, Norihisa Takei, Tomohiro Nakayama","doi":"10.1177/00045632231196045","DOIUrl":"10.1177/00045632231196045","url":null,"abstract":"Background: Although venipuncture is minimally invasive, and is the most frequently performed medical procedure, it carries the small risk of causing persistent pain, including nerve damage. Recently, our hospital stopped using 22-gauge needles for venipuncture in outpatients and switched to using only 23- and 25-gauge needles. We investigated the impact of using only the finer needles on the incidence of persistent or neuropathic pain and the prevalence of haemolysis, as well as the impact of haemolysis associated with the needle change on other laboratory data.Methods: We retrospectively collected and analysed data on venipuncture-associated pain complaints made during the 1-year period prior and 1-year period after the change in needles, as well as the frequency of haemolysis before and after the change. We also focused on 90 cases that showed significant haemolysis after the needle change and compared the serum aspartate aminotransferase, lactate dehydrogenase, and potassium levels before and after the needle change.Results: The incidence of persistent pain was significantly reduced from 1 in 10,825 venipunctures before the change to 1 in 29,747 venipunctures after the change. Notably, no patients experienced neuropathic pain after the change. However, the prevalence of haemolysis was significantly increased. Additionally, the serum aspartate aminotransferase, lactate dehydrogenase, and potassium levels were significantly elevated in the cases that showed moderate to gross haemolysis after the needle change.Conclusion: Using finer needles involves both advantages and disadvantages, and careful consideration is needed to determine which type of needle is in the best interests of the patient.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"107-114"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10457082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Practical approaches to the detection of macrotroponin. 检测大促红细胞生成素的实用方法。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-09-02 DOI: 10.1177/00045632231197301

Leo Lam, Campbell Kyle

{"title":"Practical approaches to the detection of macrotroponin.","authors":"Leo Lam, Campbell Kyle","doi":"10.1177/00045632231197301","DOIUrl":"10.1177/00045632231197301","url":null,"abstract":"Introduction: Macrotroponin is increasingly recognised as a cause of confusion in interpreting high-sensitivity cardiac troponin (hs-cTnI) results. In this study, we sought to evaluate two practical approaches to detecting macrotroponin. These two approaches are PEG precipitation and SVM (support vector machine) analysis to classify discrepancies between hs-cTn assays.Method: Residual serum and heparin plasma specimens (n = 483) with initially elevated hs-cTnI from hospital and community laboratories were retested on multiple hs-cTn platforms before and after PEG precipitation and Protein A immunoglobulin depletion. SVM analysis was conducted to identify a linear equation that best discriminated specimens with macrotroponin using a combination of results from two different hs-cTn assays.Findings: The diagnostic performance of PEG precipitation was carried out using Protein A immunoglobulin depletion as the reference comparator. When a cutoff residual activity after PEG precipitation of ≤ 20% was used, this threshold carried a high specificity of 92% (confidence interval 83-98%; n = 189) using the Siemens hs-cTnI Vista assay and 95% specificity (86%-98%; n = 242) using the Abbott hs-cTnI Architect assay. SVM analysis generated a linear equation identifying macrotroponin specimens from results obtained on two hs-cTn assays. This approach can be highly specific, comparable to PEG precipitation when certain assay combinations and concentrations are used.Conclusion: We describe and identify practical alternatives to detecting macrotroponin. These approaches can be optimised for high specificity, reducing the need for more complex laboratory methods.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"122-132"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10138562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acquired haemoglobin F (HbF) in HbA1c analysis predating a diagnosis of myelodysplastic syndrome. 骨髓增生异常综合征诊断前 HbA1c 分析中的获得性血红蛋白 F (HbF)。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-12-05 DOI: 10.1177/00045632231216605

Nathan Lorde, Farheen Karim, Rousseau Gama, Tejas Kalaria

引用次数: 0

Urine copper by colorimetry. 通过比色法检测尿铜。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-12-27 DOI: 10.1177/00045632231224244

S A Handley, T Wanandy

引用次数: 0

Development of blood collection tubes for glucose measurement using adenosine 3-phosphate and sodium fluoride as glycolytic inhibitors. 利用 3-磷酸腺苷和氟化钠作为糖酵解抑制剂，开发用于葡萄糖测量的采血管。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-08-30 DOI: 10.1177/00045632231194829

Yukio Kume, Yuji Hirowatari, Makoto Kurano, Yutaka Yatomi, Makoto Matsushita

{"title":"Development of blood collection tubes for glucose measurement using adenosine 3-phosphate and sodium fluoride as glycolytic inhibitors.","authors":"Yukio Kume, Yuji Hirowatari, Makoto Kurano, Yutaka Yatomi, Makoto Matsushita","doi":"10.1177/00045632231194829","DOIUrl":"10.1177/00045632231194829","url":null,"abstract":"Background: Blood collection tubes with sodium fluoride (NaF) added as a glycolytic inhibitor are widely used for glucose measurement. However, the glycolytic inhibitory effects of NaF are insufficient, and decreases in glucose levels over time after blood collection have become a problem.Methods: Blood from a volunteer collected using an NaF tube was used to compare the glycolysis inhibitory abilities of ATP and ADP. Blood samples from 10 volunteers were collected in NaF tubes and NaF tubes with added ATP (NaF-ATP tubes). The stability of glucose and haemoglobin (Hb)A1c after whole-blood storage from immediately after blood collection to 24 h later was compared.Results: ATP and ADP had similar inhibitory effects on glycolysis, but ATP was selected as an additive for blood collection tubes because ADP was more haemolytic than ATP. We verified the ability of NaF blood collection tubes supplemented with ATP to inhibit glycolysis. Mean (± standard deviation) glucose levels (n=10) after storage for 24 h after blood collection decreased to -9.0 ± 2.7 mg/dL (-0.50 ± 0.15 mmol/L) in conventional NaF tubes. NaF-ATP(20) tubes with 20 mg (0.036 mmol) ATP added showed a reduced decrease, with a mean of -5.8 ± 2.9 mg/dL (-0.32 ± 0.16 mmol/L). NaF-ATP tubes also had no effect on HbA1c measurement.Conclusion: This study reports on a blood collection tube that enables the measurement of glucose and HbA1c. Based on the results of validation, we conclude that NaF-ATP tubes can reduce decreases in glucose over time in stored whole blood compared to conventional NaF tubes.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"90-97"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10472854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different serum sodium assay, different model for end stage liver disease - sodium scores in patients awaiting liver transplant: A cross-sectional study. 不同的血清钠测定，不同的肝病晚期模型--等待肝移植患者的钠评分：一项横断面研究。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-08-20 DOI: 10.1177/00045632231196052

Fatima Rodriguez-Alvarez, Paulina Moctezuma-Velázquez, Blanca Zuleyma Mota-Ayala, Paul Alonso Pamila-Tecuautzin, Ignacio García-Juárez, Carlos Moctezuma-Velázquez

{"title":"Different serum sodium assay, different model for end stage liver disease - sodium scores in patients awaiting liver transplant: A cross-sectional study.","authors":"Fatima Rodriguez-Alvarez, Paulina Moctezuma-Velázquez, Blanca Zuleyma Mota-Ayala, Paul Alonso Pamila-Tecuautzin, Ignacio García-Juárez, Carlos Moctezuma-Velázquez","doi":"10.1177/00045632231196052","DOIUrl":"10.1177/00045632231196052","url":null,"abstract":"Introduction and aims: Sodium can be measured with direct or indirect methods; abnormal plasma total protein concentration can impact on sodium measured by indirect ion-selective electrodes (ISE). Serum sodium is an important item to determine the Model for End Stage Liver Disease Sodium (MELD-Na) score, commonly used for liver graft allocation. Patients with cirrhosis usually have hypoproteinemia. The aim of this study was to determine if there was a significant difference between the MELD-Na scores calculated based on the results of two different serum sodium ISE: indirect and direct.Methods: This was a retrospective study; we included 166 patients that underwent liver transplant assessment, and that had paired (i.e. same date and time) direct and indirect sodium determinations. We calculated the MELD-Na scores with both sodium determinations, and we compared them.Results: There was a significant difference between MELD-Na scores; the mean difference was 0.4±1.3. If MELD-Na score had been determined by the sodium measured by the direct ISE, 69 patients (42%) would have stayed in the same place on the waiting list, 67 patients (40%) would have moved up, and 30 patients (18%) would have moved down.Conclusions: There was a statistically significant difference between the MELD-Na scores calculated based on the two different sodium concentrations, which would theoretically result in changes in the order of the waiting list. This finding should prompt studies to assess if MELD-Na calculated based on direct methods has a better performance to predict clinically relevant outcomes.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"115-121"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10938476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10030471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Case report: Skin protective barrier cream interference in benzethonium chloride method for urine protein measurement in a 6-month-old girl. 病例报告:皮肤防护屏障霜干扰苯并索氯铵法测定6月龄女童尿蛋白。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-11-26 DOI: 10.1177/00045632231218833

Ömer Özcan, Joanna Ae van Wijk, Annet M Bosch, Wendy Pj den Elzen, Annemieke C Heijboer, Johan C Fischer

引用次数: 0

Current practice and recommendations for managing transgender patient data in clinical laboratories in the United Kingdom and Republic of Ireland. 英国和爱尔兰共和国临床实验室管理变性患者数据的现行做法和建议。

IF 2.2 4区医学

Annals of Clinical Biochemistry Pub Date : 2024-03-01 Epub Date: 2023-08-30 DOI: 10.1177/00045632231195484

Sophie Hepburn, Devon Buchanan, Seán J Costelloe

{"title":"Current practice and recommendations for managing transgender patient data in clinical laboratories in the United Kingdom and Republic of Ireland.","authors":"Sophie Hepburn, Devon Buchanan, Seán J Costelloe","doi":"10.1177/00045632231195484","DOIUrl":"10.1177/00045632231195484","url":null,"abstract":"Background: Transgender people may avoid seeking medical care due to previous negative experiences and fear of discrimination. Clinical laboratories can contribute to a poor patient experience and clinical outcome when the design and functionality of laboratory information management systems (LIMS) do not consider the needs of transgender patients. This survey aimed to capture current practices in United Kingdom and Republic of Ireland clinical laboratories concerning how transgender patient data and test requests are managed throughout the total testing process.Methods: An anonymous survey was distributed to clinical laboratory professionals in November 2021. Thirty-three questions covered how gender variables are recorded for transgender patients and used to inform gender-specific calculations, test access, and reference intervals (RIs).Results: Of the 66 respondents, 70% were based in laboratories in England, with a majority of laboratories having ISO 15189 accreditation and processing 1000-10,000 blood samples daily. Eighty-five percent stated that their LIMS had a single field recording sex or gender information. Forty-three percent did not limit test access based on gender, but 68% did not append RIs for patients with unknown or indeterminate gender.Conclusions: This survey was the first to quantify how clinical laboratories manage sex and gender information and report results for transgender and non-binary patients, and details several key recommendations based on the survey responses.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"98-106"},"PeriodicalIF":2.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10173094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0