Annals of Biomedical Engineering最新文献_第2页

Peaks and Distributions of White Matter Tract-related Strains in Bicycle Helmeted Impacts: Implication for Helmet Ranking and Optimization.

IF 3 2区医学

Annals of Biomedical Engineering Pub Date : 2024-12-05 DOI: 10.1007/s10439-024-03653-3

Zhou Zhou, Madelen Fahlstedt, Xiaogai Li, Svein Kleiven

{"title":"Peaks and Distributions of White Matter Tract-related Strains in Bicycle Helmeted Impacts: Implication for Helmet Ranking and Optimization.","authors":"Zhou Zhou, Madelen Fahlstedt, Xiaogai Li, Svein Kleiven","doi":"10.1007/s10439-024-03653-3","DOIUrl":"https://doi.org/10.1007/s10439-024-03653-3","url":null,"abstract":"Traumatic brain injury (TBI) in cyclists is a growing public health problem, with helmets being the major protection gear. Finite element head models have been increasingly used to engineer safer helmets often by mitigating brain strain peaks. However, how different helmets alter the spatial distribution of brain strain remains largely unknown. Besides, existing research primarily used maximum principal strain (MPS) as the injury parameter, while white matter fiber tract-related strains, increasingly recognized as effective predictors for TBI, have rarely been used for helmet evaluation. To address these research gaps, we used an anatomically detailed head model with embedded fiber tracts to simulate fifty-one helmeted impacts, encompassing seventeen bicycle helmets under three impact locations. We assessed the helmet performance based on four tract-related strains characterizing the normal and shear strain oriented along and perpendicular to the fiber tract, as well as the prevalently used MPS. Our results showed that both the helmet model and impact location affected the strain peaks. Interestingly, we noted that different helmets did not alter strain distribution, except for one helmet under one specific impact location. Moreover, our analyses revealed that helmet ranking outcome based on strain peaks was affected by the choice of injury metrics (Kendall's Tau coefficient: 0.58-0.93). Significant correlations were noted between tract-related strains and angular motion-based injury metrics. This study provided new insights into computational brain biomechanics and highlighted the helmet ranking outcome was dependent on the choice of injury metrics. Our results also hinted that the performance of helmets could be augmented by mitigating the strain peak and optimizing the strain distribution with accounting the selective vulnerability of brain subregions and more research was needed to develop region-specific injury criteria.","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immediate Irradiation Induced Cerebral Water and Hemodynamic Response in Whole Brain Radiotherapy.

IF 3 2区医学

Annals of Biomedical Engineering Pub Date : 2024-12-04 DOI: 10.1007/s10439-024-03663-1

Heli Miettinen, Jesse Lohela, Sadegh Moradi, Kalle Inget, Juha Nikkinen, Teemu Myllylä, Sakari S Karhula, Vesa Korhonen

{"title":"Immediate Irradiation Induced Cerebral Water and Hemodynamic Response in Whole Brain Radiotherapy.","authors":"Heli Miettinen, Jesse Lohela, Sadegh Moradi, Kalle Inget, Juha Nikkinen, Teemu Myllylä, Sakari S Karhula, Vesa Korhonen","doi":"10.1007/s10439-024-03663-1","DOIUrl":"https://doi.org/10.1007/s10439-024-03663-1","url":null,"abstract":"Purpose: Effects of clinical radiotherapy are often studied between or after irradiations. The current study's aim was to monitor an immediate irradiation response in cerebral water and hemodynamics in patients treated with whole brain radiotherapy (WBRT) and to assess the response's individuality.Methods: We used functional near-infrared spectroscopy (fNIRS) to monitor changes in cerebral water, oxyhemoglobin (HbO), and deoxyhemoglobin (HbR) during the irradiation of 31 patients (age 69.3 ± 12.5 years, 16 females) receiving WBRT. The radiation dose delivered to a patient during a single measurement was 4 Gy (total dose of 20 Gy in five fractions) for most patients and 3 Gy (total dose of 30 Gy in ten fractions) for three patients.Results: 106 patient recordings were analyzed. They showed an immediate irradiation induced increase in HbO and HbR, and decrease in cerebral water content (P < .001) as soon as 5 s after the start of irradiation. The radiation dose, age, and gender affected recorded signals. A smaller dose resulted in a steeper change in HbR (P < .01), but larger total change in HbO (P < .01). Younger age was associated with a more significant decrease in the water signal (P < .05). In contrast, female gender was associated with a greater total increase in HbO (P < .01) and HbR (P < .001) signals.Conclusion: There is an immediate cerebral water and hemodynamic response to irradiation and this response shows dependency on the radiation dose, age, and gender. Better understanding about the immediate radiation response may help improve the patient outcome in clinical radiotherapy.","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality Issues in Kinematic Traces from Three Head Impact Sensors in Boxing: Prevalence, Effects, and Implications for Exposure Assessment.

IF 3 2区医学

Annals of Biomedical Engineering Pub Date : 2024-12-03 DOI: 10.1007/s10439-024-03647-1

Enora Le Flao, Gunter P Siegmund, Seth Lenetsky, Robert Borotkanics

{"title":"Quality Issues in Kinematic Traces from Three Head Impact Sensors in Boxing: Prevalence, Effects, and Implications for Exposure Assessment.","authors":"Enora Le Flao, Gunter P Siegmund, Seth Lenetsky, Robert Borotkanics","doi":"10.1007/s10439-024-03647-1","DOIUrl":"https://doi.org/10.1007/s10439-024-03647-1","url":null,"abstract":"Purpose: When used in-vivo or in biofidelic environments, many head impact sensors have shown limitations related to the quality and validity of the kinematics measured. The objectives were to assess the quality of kinematic traces from three head impact sensors, determine the effects of signal quality on peak accelerations, and compare measurements across sensors.Methods: Head impacts were collected with instrumented mouthguards, skin patches, and headgear patches during boxing sparring. The quality of the raw kinematic traces for 442 events for each sensor was categorized using pre-defined objective criteria into high, questionable, and low-quality classes. The proportion of high-quality recordings was analyzed by participant, type of impact, and impact location. Associations between signal quality and peak kinematics were assessed within each sensor, and peak kinematics (resolved to the head center of gravity) were compared between sensors.Results: High-quality criteria were met in 53%, 20%, and 26% of events for the mouthguard, skin patch, and headgear patch, respectively. High-quality recordings were less frequent for impacts occurring close to the sensor (e.g., 30% vs. 61% for the mouthguard) and showed lower peak kinematics than low-quality recordings (p < 0.001). Despite careful selection of high-quality simultaneous recordings, there was little to no association between the sensors' measurements (Spearman's p ≥ 0.043).Conclusions: The kinematic data often reflected the motion of the sensor itself rather than the motion of the head, overestimating head impact magnitude. Researchers should evaluate data quality prior to analyzing kinematics or injury severity metrics. Comparison of data across studies or in relation to injury risk functions needs to be done with caution when data were acquired from different sensors.","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Virtual Response Surface Strategy to Predict the Effects of Contouring on the Static and Fatigue Mechanical Behavior of Spinal Rods.

IF 3 2区医学

Annals of Biomedical Engineering Pub Date : 2024-12-02 DOI: 10.1007/s10439-024-03649-z

Linda Carpenedo, Francesca Berti, Luigi La Barbera

{"title":"A Virtual Response Surface Strategy to Predict the Effects of Contouring on the Static and Fatigue Mechanical Behavior of Spinal Rods.","authors":"Linda Carpenedo, Francesca Berti, Luigi La Barbera","doi":"10.1007/s10439-024-03649-z","DOIUrl":"https://doi.org/10.1007/s10439-024-03649-z","url":null,"abstract":"Spinal fixation techniques based on contoured spinal rods are well established to restore an adequate sagittal alignment; however, they often break due to fatigue loading because residual stresses arise while deforming the material and they significantly reduce rod fatigue strength. The present paper aims at developing a novel strategy based on Response Surfaces (RS) as surrogate reduced order models to accurately predict the key biomechanical parameters involved both during static spinal rod contouring and subsequent fatigue loading. Finite element (FE) models of different contouring methods are performed on a wide range of spinal rods to reproduce typical clinical scenarios. RSs based on polynomial equations are initially used to fit the collected FE data and relate input parameters of the process to outputs mechanical variables. Then, the RS approach is assessed and validated by comparison with extensive experimental observations from a variety of fatigue tests. The RS provides an accurate prediction of the output parameters of interest (R2 = 0.88-1), with an average relative error up to 9% compared to computationally demanding FE models. Fatigue outputs are correctly predicted both for breakages and survivals of rods, despite an average overestimation on the equivalent stress of 11%. The RS approach proves credible and accurate in predicting the experimental fatigue strength of contoured spinal rods. The proposed method could serve in clinical practice as an intraoperative virtual tool to support the decision-making process.","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimating Ground Reaction Forces from Gait Kinematics in Cerebral Palsy: A Convolutional Neural Network Approach.

IF 3 2区医学

Annals of Biomedical Engineering Pub Date : 2024-11-30 DOI: 10.1007/s10439-024-03658-y

Mustafa Erkam Ozates, Firooz Salami, Sebastian Immanuel Wolf, Yunus Ziya Arslan

{"title":"Estimating Ground Reaction Forces from Gait Kinematics in Cerebral Palsy: A Convolutional Neural Network Approach.","authors":"Mustafa Erkam Ozates, Firooz Salami, Sebastian Immanuel Wolf, Yunus Ziya Arslan","doi":"10.1007/s10439-024-03658-y","DOIUrl":"https://doi.org/10.1007/s10439-024-03658-y","url":null,"abstract":"Purpose: While gait analysis is essential for assessing neuromotor disorders like cerebral palsy (CP), capturing accurate ground reaction force (GRF) measurements during natural walking presents challenges, particularly due to variations in gait patterns. Previous studies have explored GRF prediction using machine learning, but specific focus on patients with CP is lacking. This research aims to address this gap by predicting GRF using joint angles derived from marker data during gait in patients with CP, thereby suggesting a protocol for gait analysis without the need for force plates.Methods: The study employed an extensive dataset comprising both typically developed (TD) subjects (n = 132) and patients with CP (n = 622), captured using motion capture systems and force plates. Kinematic data included lower limb angles in three planes of motion, while GRF data encompassed three axes. A one-dimensional convolutional neural network model was designed to extract features from kinematic time series, followed by densely connected layers for GRF prediction. Evaluation metrics included normalized root mean squared error (nRMSE) and Pearson correlation coefficient (PCC).Results: GRFs of patients with CP were predicted with nRMSE values consistently below 20.13% and PCC scores surpassing 0.84. In the TD group, all GRFs were predicted with higher accuracy, showing nRMSE values lower than 12.65% and PCC scores exceeding 0.94.Conclusion: The predictions considerably captured the patterns observed in the experimentally obtained GRFs. Despite limitations, including the absence of upper extremity kinematics data and the need for continuous model evolution, the study demonstrates the potential of machine learning in predicting GRFs in patients with CP, albeit with current prediction errors constraining immediate clinical applicability.","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decorin Knockdown Improves Aged Tendon Healing by Enhancing Recovery of Viscoelastic Properties, While Biglycan May Not.

IF 3 2区医学

Annals of Biomedical Engineering Pub Date : 2024-11-29 DOI: 10.1007/s10439-024-03612-y

Christelle Darrieutort-Laffite, Stephanie N Weiss, Courtney A Nuss, Joseph B Newton, Jeremy D Eekhoff, Louis J Soslowsky

{"title":"Decorin Knockdown Improves Aged Tendon Healing by Enhancing Recovery of Viscoelastic Properties, While Biglycan May Not.","authors":"Christelle Darrieutort-Laffite, Stephanie N Weiss, Courtney A Nuss, Joseph B Newton, Jeremy D Eekhoff, Louis J Soslowsky","doi":"10.1007/s10439-024-03612-y","DOIUrl":"https://doi.org/10.1007/s10439-024-03612-y","url":null,"abstract":"The objective of the study was to determine the specific roles of decorin and biglycan in the early and late phases of tendon healing in aged mice. Aged (300 day-old) female wildtype (WT), Dcnflox/flox (I-Dcn-/-), Bgnflox/flox (I-Bgn-/-), and compound Dcnflox/flox/Bgnflox/flox (I-Dcn-/-/Bgn-/-) mice with a tamoxifen (TM) inducible Cre underwent a bilateral patellar tendon injury (PT). Cre excision of the conditional alleles was induced at 5 days (samples collected at 3 and 6 weeks) or 21 days post-injury (samples collected at 6 weeks). Scar tissue area, collagen architecture, gene expression and mechanical properties were assessed during re-establishment of tendon architecture after injury. Fibril diameter distribution was impacted by both decorin and biglycan knockdown at 3 and 6 weeks compared to WT. Although early healing appeared to be delayed in the I-Bgn-/- tendons (larger scar tissue area at 3 weeks), no differences in failure properties were detected. By 6 weeks, in the I-Dcn-/- tendons, we observed a better recovery of viscoelastic properties compared to the WT tendons (reduced stress relaxation and increased dynamic modulus) when the knockdown was induced early. This could be explained by the increased expression of other matrix proteins, such as elastin whose gene expression was increased at 3 weeks in the I-Dcn-/- tendons. Despite an impact on collagen fibrillogenesis, decorin and/or biglycan knockdown did not produce a detectable effect on quasi-static properties after patellar tendon injury. However, early decorin knockdown resulted in better recovery of viscoelastic properties. Mechanisms underlying this result remained to be clarified in further studies.","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Motorpoint Heatmap of the Hamstring Muscles to Facilitate Neuromuscular Electrical Stimulation.

IF 3 2区医学

Annals of Biomedical Engineering Pub Date : 2024-11-28 DOI: 10.1007/s10439-024-03657-z

J Flodin, P Amiri, R Juthberg, P W Ackermann

{"title":"Motorpoint Heatmap of the Hamstring Muscles to Facilitate Neuromuscular Electrical Stimulation.","authors":"J Flodin, P Amiri, R Juthberg, P W Ackermann","doi":"10.1007/s10439-024-03657-z","DOIUrl":"https://doi.org/10.1007/s10439-024-03657-z","url":null,"abstract":"The effectiveness of neuromuscular electrical stimulation (NMES) depends on electrode placement, with placement over the motor points (MPs) being the most effective. This study aimed to determine the MP-distribution and establish a heatmap indicating the probability of finding a MP in different areas of the hamstring (H) muscles. Additionally, inter-individual variations in the number of MPs were investigated. Thirty-one healthy participants (15 females, 16 males), aged 18-65 years, were included. The individual anatomy of the H-muscles, including the biceps femoris (BF), semitendinosus (ST), and semimembranosus (SM), was determined using ultrasound. MPs were located using a MP-search pen. The thigh anatomy was divided into 70 3x3cm areas, and the probability of finding a MP in each area was calculated using the Clopper-Pearson test to create a heatmap. Regression analysis was employed to determine if patient characteristics were associated with the number of MPs. The two best areas, found over BF and ST, exhibited a 39% probability of containing a MP and were significantly more likely to contain a MP compared to 85% of the remaining areas (p < 0.05). Two areas over SM had a 29% probability of containing a MP. BF exhibited a significantly higher number of MPs compared to SM and ST (p < 0.001). Male sex and higher physical activity were independent explanatory factors positively correlated with the number of MPs over BF in the multiple linear regression (R2 = 0.38, p = 0.001). The MP-heatmap of the H-muscles can effectively facilitate NMES application by highlighting areas with a higher probability of finding a MP. Large inter-individual variations in location and number of MPs were demonstrated.","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic Deformation Behavior of the Porcine Anterior Cruciate Ligament Enthesis Under Anterior Tibial Loading. 猪前交叉韧带假体在胫骨前部负荷下的动态变形行为

IF 3 2区医学

Annals of Biomedical Engineering Pub Date : 2024-11-27 DOI: 10.1007/s10439-024-03654-2

Daichi Ishii, Shiho Sato, Hiromichi Fujie

{"title":"Dynamic Deformation Behavior of the Porcine Anterior Cruciate Ligament Enthesis Under Anterior Tibial Loading.","authors":"Daichi Ishii, Shiho Sato, Hiromichi Fujie","doi":"10.1007/s10439-024-03654-2","DOIUrl":"https://doi.org/10.1007/s10439-024-03654-2","url":null,"abstract":"This study determined the insertion angle at the porcine anterior cruciate ligament (ACL) enthesis under joint loading to provide information on the structure and mechanical function of the enthesis. Ten intact porcine knee joints were harvested, and an anterior tibial load was applied using a robotic testing system. After dissecting a portion of the ACL enthesis along ligament fibers, the remaining enthesis was imaged using a digital microscope while reproducing the three-dimensional intact knee motion. Fiber orientation angles (FOAs) in the enthesis region (0-300 µm from the ligament-bone boundary) and the ligament region (500-2000 µm from the ligament-bone boundary) were analyzed in the femoral and tibial entheses of the anteromedial bundle (AMB) of the ACL under loading. On the femoral side, the FOA in the enthesis region was significantly higher than that in the ligament region by approximately 10 degrees under loading (n = 5, p < 0.05 in paired t-test). In contrast, the FOAs in the enthesis and ligament regions on the tibial side were nearly equal under loading, with no significant difference (n = 5, p > 0.15 in paired t-test). Histological examination indicated that uncalcified fibrocartilage (UF) was abundant in the enthesis region of the AMB femoral enthesis while the UF was not observed in the enthesis region of the AMB tibial enthesis. Thus, the current data suggest that the regional dependence and independence in FOA are caused by the presence or absence of UF and contributes to a moderate and subtle load-transduction in the ACL enthesis.","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adapting ChatGPT for Color Blindness in Medical Education. 在医学教育中针对色盲改编 ChatGPT。

IF 3 2区医学

Annals of Biomedical Engineering Pub Date : 2024-11-27 DOI: 10.1007/s10439-024-03656-0

Jinge Wang, Thomas C Yu, Michael S Kolodney, Peter L Perrotta, Gangqing Hu

引用次数: 0

Engineered Osteochondral Scaffolds with Bioactive Cartilage Zone for Enhanced Articular Cartilage Regeneration. 具有生物活性软骨区的工程骨软骨支架用于增强关节软骨再生。

IF 3 2区医学

Annals of Biomedical Engineering Pub Date : 2024-11-27 DOI: 10.1007/s10439-024-03655-1

Aleksandra A Golebiowska, Jonathon T Intravaia, Vinayak Sathe, Sangamesh G Kumbar, Syam P Nukavarapu

{"title":"Engineered Osteochondral Scaffolds with Bioactive Cartilage Zone for Enhanced Articular Cartilage Regeneration.","authors":"Aleksandra A Golebiowska, Jonathon T Intravaia, Vinayak Sathe, Sangamesh G Kumbar, Syam P Nukavarapu","doi":"10.1007/s10439-024-03655-1","DOIUrl":"https://doi.org/10.1007/s10439-024-03655-1","url":null,"abstract":"Despite progress, osteochondral (OC) tissue engineering strategies face limitations in terms of articular cartilage layer development and its integration with the underlying bone tissue. The main objective of this study is to develop a zonal OC scaffold with native biochemical signaling in the cartilage zone to promote articular cartilage development devoid of cells and growth factors. Herein, we report the development and in vivo assessment of a novel gradient and zonal-structured scaffold for OC defect regeneration. The scaffold system is composed of a mechanically supportive 3D-printed template containing decellularized cartilage extracellular matrix (ECM) biomaterial in the cartilage zone that possesses bioactive characteristics, such as chemotactic activity and native tissue biochemical composition. OC scaffolds with a bioactive cartilage zone were implanted in vivo in a rabbit osteochondral defect model and assessed for gross morphology, matrix deposition, cellular distribution, and overall tissue regeneration. The scaffold system supported recruitment and infiltration of host cells into the cartilage zone of the graft, which led to increased ECM deposition and physiologically relevant articular cartilage tissue formation. Semi-quantitative ICRS scoring (overall score double for OC scaffold with bioactive cartilage zone compared to PLA scaffold) further confirm the bioactive scaffold enhanced articular cartilage engineering. This strategy of designing bioactive scaffolds to promote endogenous cellular infiltration can be a much simpler and effective approach for OC tissue repair and regeneration.","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0