Annals of Biomedical Engineering最新文献

{"title":"Exploring Manufacturing Techniques in Bioceramic Scaffold Fabrication with a Focus on DIW 3D Printing for Tissue Engineering Applications.","authors":"D L Belgin Paul, Praveen Ayyappan Susila, M Karthick","doi":"10.1007/s10439-025-03722-1","DOIUrl":"https://doi.org/10.1007/s10439-025-03722-1","url":null,"abstract":"<p><p>In recent years, notable advancements have been achieved in the domain of tissue engineering and regenerative medicine, presenting auspicious remedies for the management of diverse injuries and ailments. The management of bone injuries necessitates the implementation of a customized approach depending upon the specific nature of the injury. Tissue engineering plays a major role in the treatment of such bone injuries. One key aspect of tissue engineering is the development of scaffolds that can provide structural support and guide the growth of new tissue. The scaffold should possess mechanical properties that enable it to withstand weight-bearing stresses, resembling the strength and durability of real bone. This review explores the latest research findings from pioneering research papers in tissue engineering, focusing on scaffolds, bioceramics, and the various technique for the development of scaffolds with a focus on 3D printing. By examining and synthesizing key studies in these areas, this review aims to provide insights into the current state of knowledge, identify research gaps, and contribute to the advancement of tissue engineering approaches for regenerative medicine applications.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Regional Mapping of Articular Cartilage Integrity and Biphasic Properties in Healthy and Osteoarthritic Trapeziometacarpal Joints.","authors":"Lizzie Walker, Hui Li, Nathan Buchweitz, Daniel Gordon, Shangping Wang, Dane Daley, Hai Yao, Yongren Wu","doi":"10.1007/s10439-025-03726-x","DOIUrl":"https://doi.org/10.1007/s10439-025-03726-x","url":null,"abstract":"<p><strong>Purpose: </strong>To identify the mechanical and morphological regional changes of articular cartilage on the first metacarpal and trapezium and their association with attenuation of the volar ligament complex (VLC) during trapeziometacarpal (TMC) osteoarthritis (OA) progression.</p><p><strong>Methods: </strong>Twenty-four fresh-frozen female cadaveric TMCs were separated into (1) younger healthy/early-stage osteoarthritic, (2) elder healthy/early-stage osteoarthritic, and (3) advanced-stage osteoarthritic groups based on age and Eaton-Littler grading. Metacarpal and trapezium surfaces were split into six regions. Microindentation testing was performed to characterize the biphasic properties of each region. Light imaging, scanning electron microscopy/energy dispersive spectroscopy, and magnetic resonance imaging were performed to assess cartilage integrity and identify wear patterns.</p><p><strong>Results: </strong>The volar ulnar region of the metacarpal, along with the volar central and volar ulnar regions of the trapezium, had a higher equilibrium modulus in the advanced-stage OA specimens. SEM/EDS revealed these regions to be fully eburnated in most cases. MRI revealed eburnation, as well as the degeneration and/or detachment of the beak ligament portion of the VLC. In the advanced-stage OA TMCs, an increased equilibrium modulus in the volar portion of the trapezium correlates to attenuated VLC stiffness from our previous study.</p><p><strong>Conclusion: </strong>A regional pattern in articular cartilage degeneration, including changes in biphasic properties evidenced by an increased equilibrium modulus, during TMC OA progression is evident with the most significant changes occurring in the volar ulnar region of the metacarpal and volar central and volar ulnar regions of the trapezium. These changes in articular cartilage can be correlated to an attenuated VLC.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vascular Flow Phantom of A Cohort-Based Averaged Abdominal Aortic Aneurysm: Design, Fabrication and Characterization.","authors":"H Mirgolbabaee, J R Nagel, J Plomp, A Ghanbarzadeh-Dagheyan, J A Simmering, M Versluis, M M P J Reijnen, E Groot Jebbink","doi":"10.1007/s10439-025-03717-y","DOIUrl":"https://doi.org/10.1007/s10439-025-03717-y","url":null,"abstract":"<p><strong>Purpose: </strong>Vascular flow phantoms are an invaluable tool for in vitro and in silico studies, but their design and fabrication processes are often not reported. In this study, a framework is introduced to design and fabricate 3D printable high-fidelity cohort-based averaged abdominal aortic aneurysm (AAA) phantoms.</p><p><strong>Methods: </strong>AAA geometries of 50 patients were segmented from preoperative computed tomography angiography scans. The segmented geometries and center lumen lines (CLL) were used in an in-house developed algorithm to average the CLL coordinates and corresponding diameters over the entire cohort. The reconstructed averaged anatomy was 3D printed as a thin-walled flow phantom with Formlabs Flexible 80A resin. The acoustic properties of the resin were characterized and the feasibility of flow field quantification inside the phantom with ultrasound particle imaging velocimetry (echoPIV) was investigated.</p><p><strong>Results: </strong>Comparison between patient-specific models generated by our method and their corresponding reference segmentations, for ten patients, showed a mean Sørensen-Dice similarity coefficient of 0.916 ± 0.21 and the largest distances (5-10% of the lumen diameter) were found at the aneurysmal sac. The Flexible 80A resin had an average speed of sound of 1785 m/s, attenuation of 7.8 dB/mm and density of 1130 kg/m³. Volumetric flow profiles obtained with echoPIV in the suprarenal artery (i.e. phantom inlet) matched the flow sensor data.</p><p><strong>Conclusion: </strong>The reported framework was used to make an averaged, cohort-based AAA model, which showed a good match with its reference model. A 3D printed, thin-walled phantom was made based on this model and the feasibility of flow field quantification inside the phantom was shown.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Structural Changes in Extracorporeal Membrane Oxygenation Return Cannulas on Hemodynamic Performance and Blood Damage Associated with Cannulation.","authors":"Yifeng Xi, Yuan Li, Hongyu Wang, Xiaofei Wang, Wentao Feng, Zengsheng Chen","doi":"10.1007/s10439-025-03720-3","DOIUrl":"https://doi.org/10.1007/s10439-025-03720-3","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to investigate the effects of different arterial cannula models on hemodynamic performance and blood damage associated with femoral artery cannulation in venoarterial extracorporeal membrane oxygenation (VA-ECMO).</p><p><strong>Methods: </strong>Eleven cannula models were constructed and processed to study their hydrodynamic performance and hemolysis in a circulated loop. All circulation environments were analyzed using computational fluid dynamics to investigate hemodynamic changes under different ECMO flow conditions.</p><p><strong>Results: </strong>The multiple side-hole cannula structure effectively reduces the cannula pressure drop and ECMO blood pumping rate compared to cannula without side holes, thereby reducing overall blood damage in the ECMO circulation. The cannula pressure drop decreased with increasing number of side holes and became the lowest in the four and six-side-hole cannula models. A gradual increase in the number of cannula side holes improved the lower limb blood diversion ratio of ECMO, and this increase was less pronounced with a higher number of side holes. Adding a lower-extremity diversion hole can further increase the level of lower-extremity perfusion. The overall hemolytic damage in the ECMO circuit decreased gradually with an increasing number of cannula-side holes, reaching to the lowest levels in the 4 and 6-side hole cannulation models. The lower extremity blood flow rate reduced after the cannula was implanted into the vessel, forming an area of high blood retention and platelet activation in the cannula vicinity, with a greater risk of thrombosis.</p><p><strong>Conclusion: </strong>Cannula structure plays an important role in determining ECMO limb perfusion distribution, hemolysis, and thrombosis risk. A modest increase in the number of cannula side holes and cannula size could improve lower-limb perfusion and reduce the risk of hemolysis and thrombosis. Adding a lower limb diversion structure to a multiple side-hole cannula can further improve lower extremity diversion and reduce the risk of hemolysis and thrombosis. The findings of this study can provide guidance for optimizing the design of cannula configuration and improving cannula-related blood compatibility.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-Contrast Agent with Nanoparticle and Molecular Components in Photon-Counting Computed Tomography: Assessing Articular Cartilage Health.","authors":"Petri Paakkari, Satu I Inkinen, Jiri Jäntti, Juuso Tuppurainen, Maria C Fugazzola, Anisha Joenathan, Sampo Ylisiurua, Miika T Nieminen, Heikki Kröger, Santtu Mikkonen, René van Weeren, Brian D Snyder, Juha Töyräs, Miitu K M Honkanen, Hanna Matikka, Mark W Grinstaff, Juuso T J Honkanen, Janne T A Mäkelä","doi":"10.1007/s10439-025-03715-0","DOIUrl":"https://doi.org/10.1007/s10439-025-03715-0","url":null,"abstract":"<p><strong>Purpose: </strong>Photon-counting detectors (PCDs) are cutting-edge technology that enable spectral computed tomography (CT) imaging with a single scan. Spectral imaging is particularly effective in contrast-enhanced CT (CECT) imaging, especially when multiple contrast agents are utilized, as materials are distinguishable based on their unique X-ray absorption. One application of CECT is joint imaging, where it assesses the structure and composition of articular cartilage soft tissue. This evaluates articular cartilage and reveals compositional changes associated with early-stage osteoarthritis (OA) using a photon-counting detector CT (PCD-CT) technique combined with a dual-contrast agent method.</p><p><strong>Methods: </strong>A dual-contrast agent combination was used, consisting of proteoglycan-binding cationic tantalum oxide nanoparticles, developed in our lab, and a commercial non-ionic iodinated iodixanol agent. Ex vivo equine stifle joint cartilage samples (N = 30) were immersed in the contrast agent bath for 96 hours and imaged at multiple timepoints for analysis of proteoglycan, collagen, and water contents as well as collagen orientation, histological scoring, and biomechanical parameters.</p><p><strong>Results: </strong>By analyzing contrast agent concentrations, the technique provided a simultaneous assessment of the solid constituents and function of cartilage. Contrast agent diffusion depended on contrast agent composition and was significantly different between healthy and early-stage OA groups within 12 hours.</p><p><strong>Conclusion: </strong>The present study shows the promising utility of the dual-contrast PCD-CT technique for articular cartilage assessment and early-stage OA detection.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field-Ready Suction Solutions for Emergencies: The Battlefield Ready Innovative Suction Kit (BRISK).","authors":"Saketh R Peri, Maria J Londono, Rakib Hasan, Connor J Evans, Robert A De Lorenzo, R Lyle Hood","doi":"10.1007/s10439-025-03700-7","DOIUrl":"https://doi.org/10.1007/s10439-025-03700-7","url":null,"abstract":"<p><strong>Introduction: </strong>Portable suction devices are crucial for emergency airway management. Commercially-available units are unsuitable for field use due to size and power needs. A light-weight and multi-orientation operable portable suction device, Battlefield Ready Innovative Suction Kit (BRISK) was developed. The design was informed by feedback from combat medics, paramedics, and EMTs.</p><p><strong>Methods: </strong>End-user engagement and feedback defined BRISK's design. The fabricated prototype used a vacuum pump and hydrophobic syringe filters. Performance tests measured vacuum pressure, air and liquid (water and ISO vomit simulant) flow rates, volume of water suctioned in different orientations (upright, tilted, or inverted), and contamination prevention between BRISK, SSCOR Quickdraw, and Laerdal LCSU4.</p><p><strong>Results: </strong>The BRISK device-weighing 0.97 kg-demonstrated a maximum vacuum pressure of 570 ± 6 mmHg and an air flowrate of 5.20 L/min. Liquid flow rates (L/min) for BRISK, LCSU4, and SSCOR with water were 4.92 ± 0.2, 6.97 ± 0.1, and 5.37 ± 0.1, respectively. With ISO vomit simulant, the rates were 3.23 ± 0.2, 3.06 ± 0.4, and 2.23 ± 0.1. BRISK showed consistent performance across orientations (p = 0.081), while LCSU4 and SSCOR varied significantly (p < 0.0001). The BRISK's cross-contamination between filters and the pump was 0.01%, which is far less than the  rated contamination level of 0.1% as described by ISO 10079-1.</p><p><strong>Conclusion: </strong>BRISK is 30% lighter and achieves competitive vacuum pressures and liquid flow rates than comparable units currently in the market. It ensures effective evacuation of liquids in all orientations: upright, tilted, or inverted. These results demonstrate the BRISK has the potential to provide superior clinical performance in pre-hospital and first response scenarios.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic Fracture Fixation Plates: A Systematic Review of Evolving Design Approaches.","authors":"Connor Huxman, April Armstrong, Gary Updegrove, Gregory S Lewis, Jared Butler","doi":"10.1007/s10439-025-03714-1","DOIUrl":"https://doi.org/10.1007/s10439-025-03714-1","url":null,"abstract":"<p><p>Fracture fixation with standard locked plates can suppress interfragmentary motion beneficial for secondary bone healing. To address this limitation, dynamic fracture fixation plates have been developed which seek to maintain bending and torsional rigidity while providing controlled axial micromotion. This article provides a comprehensive systematic review of the history and current state of proposed dynamic plating technologies to better inform future development. 59 records (51 articles, 8 patents) describing 26 unique dynamic plating devices were identified across three literature and patent databases using PRISMA review guidelines. Concepts were grouped into one of 9 engineering approach categories, including plates that incorporate sliding mechanisms, elastic inserts, lattice structures, and mechanically compliant flexures, among others. Devices are compared in their technological characteristics, ranges of axial motion, stiffnesses, and levels of development. Despite many dynamic technologies demonstrating good healing results experimentally and clinically, widespread clinical adoption has not occurred. Some explanations for this are provided, including production costs for complex designs and the current co-existence of both rigid and flexible fixation approaches. Overall, dynamic plating offers a promising area of innovation to address the ongoing concerns of non-union rates associated with standard locked plating of long bone fractures.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional Magnetic Nanoparticles for Targeted Drug Delivery Against Cancer: A Review of Mechanisms, Applications, Consequences, Limitations, and Tailoring Strategies.","authors":"Shumaila Riaz, Shaukat Ali, Muhammad Summer, Urva Akhtar, Shehzeen Noor, Rimsha Haqqi, Muhammad Adeel Farooq, Iqra Sardar","doi":"10.1007/s10439-025-03712-3","DOIUrl":"https://doi.org/10.1007/s10439-025-03712-3","url":null,"abstract":"<p><p>Magnetic nanoparticles (MNPs) have revolutionized cancer therapy by serving as effective drug transporters through active and passive targeting of tumor sites in conjugation with external alternating magnetic fields (AMFs), thus minimizing off-target effects. This precise targeting strategy guarantees a focused and controlled drug release at the tumor site, reducing the drawbacks of standard drug delivery systems and enhancing treatment effectiveness. Magnetic nanoparticles usually follow in magnetic hyperthermia (MHT) therapy, where AMFs raise the temperature at the tumor site, efficiently eliminating cancer cells and presenting a hopeful complement to conventional cancer treatments. In addition, side effects are reduced by launching a smart drug delivery system (SDDSs) in which treatment efficacy is enhanced by reducing the dosage frequency. Intrinsic properties of MNPs are measured when they serve as contrast agents in magnetic resonance imaging (MRI), providing a diagnostic aspect to their therapeutic capabilities and enabling medical professionals to monitor and record treatment outcomes with precision and higher accuracy. This comprehensive review highlights the multifaceted potential of MNPs in reshaping cancer treatment, emphasizing their role in targeted drug delivery, hyperthermia therapy, and imaging applications, and underscoring their transformative impact on the future of oncological care.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EMG-Informed Neuromusculoskeletal Simulations Increase the Accuracy of the Estimation of Knee Joint Contact Forces During Sub-optimal Level Walking.","authors":"Domitille Princelle, Marco Viceconti, Giorgio Davico","doi":"10.1007/s10439-025-03713-2","DOIUrl":"https://doi.org/10.1007/s10439-025-03713-2","url":null,"abstract":"<p><strong>Purpose: </strong>Personalized musculoskeletal models are crucial to get insights into the mechanisms underpinning neuromusculoskeletal disorders and have the potential to support clinicians in the daily management and evaluation of patients. However, their use is still limited due to the lack of validation studies, which hinders people's trust in these technologies. The current study aims to assess the predictive accuracy of two common approaches to estimate knee joint contact forces, when employing musculoskeletal models.</p><p><strong>Methods: </strong>Subject-specific musculoskeletal models were developed for four elderly subjects, exploiting the freely accessible Knee Grand Challenge datasets, and used to perform biomechanical simulations of level walking to estimate knee joint contact forces. The classical static optimization and EMG-assisted approaches were implemented to resolve the muscle redundancy problem. Their estimates were compared, in terms of predictive accuracy, against the experimental recordings from an instrumented knee implant and against one another. Spatiotemporal differences were identified through Statistical Parametrical Mapping, to complement traditional similarity metrics (R², RMSE, 95th percentile, and the maximal error).</p><p><strong>Results: </strong>Both methods allowed to estimate the experimental knee joint contact forces experienced during walking with a high level of accuracy (R² > 0.82, RMSE < 0.56 BW). The EMG-assisted approach further enabled to highlight subject-specific features that were not captured otherwise, such as a prolonged or anticipated muscle-co-contraction.</p><p><strong>Conclusion: </strong>While the static optimization approach provides reasonable estimates for subjects exhibiting typical gait, the EMG-assisted approach should be preferred and employed when studying clinical populations or patients exhibiting abnormal walking patterns.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical and Compositional Changes in the Murine Uterus with Age.","authors":"Mari J E Domingo, Triniti N Vanoven, Raffaella De Vita, Maria E Florian Rodriguez, Kristin S Miller, Isaac J Pence","doi":"10.1007/s10439-025-03709-y","DOIUrl":"https://doi.org/10.1007/s10439-025-03709-y","url":null,"abstract":"<p><p>The uterus is a hollow, fibromuscular organ involved in physiological processes such as menstruation and pregnancy. The content and organization of extracellular matrix constituents such as fibrillar collagen dictate passive (non-contractile) biomechanical tissue function; however, how extracellular matrix composition and biomechanical function change with age in the uterus remains unknown. This study utilizes Raman spectroscopy coupled with biaxial inflation testing to investigate changes in the murine uterus with age (2-3 months, 4-6 months, 10-12 months, and 20-24 months). Linear and toe moduli significantly decreased with reproductive aging (2 to 12 months); however, both moduli increased in the oldest age group (20-24 months). The optical concentration of the combined elastin and collagen spectrum was significantly higher in the oldest group (20-24 month), while the glycogen contribution was the highest in the 2-3 month murine uterus. The presented workflow couples biaxial inflation testing and Raman spectroscopy, representing a critical first step to correlating biomechanics and optical signatures in the aging uterus with the potential for clinical translation. Further, this study may provide critical compositional and structure-function information regarding age-related uterine disorders.</p>","PeriodicalId":7986,"journal":{"name":"Annals of Biomedical Engineering","volume":" ","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}