Annals of Applied Biology最新文献_第9页

Enzymatic and insecticidal activities of the Amsacta moorei entomopoxvirus glycosyltransferase, AMV248 木霉属昆虫痘病毒糖基转移酶AMV248的酶活性和杀虫活性

IF 2.6 3区农林科学

Annals of Applied Biology Pub Date : 2022-10-25 DOI: 10.1111/aab.12813

Donus Gencer, Cihan Inan, Zihni Demirbag, Hacer Muratoğlu

{"title":"Enzymatic and insecticidal activities of the Amsacta moorei entomopoxvirus glycosyltransferase, AMV248","authors":"Donus Gencer, Cihan Inan, Zihni Demirbag, Hacer Muratoğlu","doi":"10.1111/aab.12813","DOIUrl":"https://doi.org/10.1111/aab.12813","url":null,"abstract":"The family Poxviridae is divided into two subfamilies, the Chordopoxvirinae of vertebrates and the Entomopoxvirinae of invertebrates. The Amsacta moorei entomopoxvirus (AMEV, Entomopoxvirinae) has the potential to be used in gene therapy, as an expression vector, and as a biopesticide. It was suggested that AMV248 protein is a putative glycosyltransferase (GT) but was also shown to be an attachment protein to host receptors. GTs encoded by some other viruses catalyse the binding of sugars molecules to growth hormones of the host insects rendering the hormones inactive. Consequently, larval development is arrested and frequently results in larval mortality. In this study, AMV248 protein was shown to be a GT and the purified enzyme catalysed the production of uridine diphosphate (UDP) from the substrates UDP-glucose and UDP-N-acetylglucosamine. This AMEV enzyme may behave much like the ecdysteroid UDP-glucosyltransferase of baculoviruses. Various concentrations of the GT enzyme were tested for its insecticidal activity against gypsy moth Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Lymantriidae), lackey moth Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae), cotton bollworm Helicoverpa armigera (Hübner, 1808) (Lepidoptera: Noctuidae) and the greater wax moth Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae) larvae. It had varying deleterious effects on all test larvae but L. dispar was the most sensitive to the enzyme. While this enzyme exhibits properties with potential to be developed as an insecticide in biocontrol strategies, investigations are needed to ascertain its value in pest management.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"182 2","pages":"238-244"},"PeriodicalIF":2.6,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50143830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection and identification of phytoplasmas associated with Catharanthus roseus, Pinus eldarica, and Petunia hybrida in southeastern Iran's urban green spaces 伊朗东南部城市绿地玫瑰花、木松和矮牵牛相关植物原体的分子检测与鉴定

IF 2.6 3区农林科学

Annals of Applied Biology Pub Date : 2022-10-11 DOI: 10.1111/aab.12810

Fatemeh Movahedi-Parizi, Akbar Hosseinipour, Mehdi Azadvar, Hossain Massumi

{"title":"Molecular detection and identification of phytoplasmas associated with Catharanthus roseus, Pinus eldarica, and Petunia hybrida in southeastern Iran's urban green spaces","authors":"Fatemeh Movahedi-Parizi, Akbar Hosseinipour, Mehdi Azadvar, Hossain Massumi","doi":"10.1111/aab.12810","DOIUrl":"10.1111/aab.12810","url":null,"abstract":"Given the potential for urban green spaces to provide fresh and healthy environments for humans, exploring the issues that threaten plants in these places is crucial. Phytoplasma-related symptoms were encountered on some plants in urban green spaces in the province of Kerman, southeastern Iran, between 2017 and 2019. Affected periwinkles and petunias exhibited phytoplasma disease symptoms, including virescence, phyllody, and witches'-broom. However, ball or disc-like shoot proliferation symptoms were noticed on the trunks and branches of pine trees. PCR was performed with phytoplasma-detecting universal primers, targetting and amplifying the 16S rRNA gene, and determining whether phytoplasmas are implicated in the symptomatic plants. The infection of the symptomatic plants was confirmed using nested-PCR amplification of expected DNA sizes for phytoplasmas. No product, however, was amplified from sampled symptomless plants. The sequencing of nested-PCR products was performed to obtain sequences encasing the standard F2nR2 fragments. The resulted sequences were submitted to iPhyClassifier, the universal phytoplasma classification platform, for the taxonomic assignment of the found phytoplasmas compared with previously identified ‘Candidatus Phytoplasma’ species, groups, and subgroups. The results revealed that phytoplasma strains related to the species ‘Ca. P. trifolii’ (16SrVI-A subgroup) infect periwinkles and pines. However, strains from the species ‘Ca. P. aurantifolia’ (16SrII-D subgroup) and ‘Ca. P. phoenicium’ (16SrIX-C subgroup) were found in petunias and periwinkles, respectively. To the best of our knowledge, phytoplasmas from the 16SrVI-A and 16SrII-D subgroups are the first reported to infect these plants in Kerman province, while a related strain from the subgroup 16SrIX-C is the first recorded to infect periwinkles in Iran and the second in the world.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"182 2","pages":"205-215"},"PeriodicalIF":2.6,"publicationDate":"2022-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46129987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flower-visitor diversity and pollination of Indian olive (Elaeocarpus floribundus Blume) 印度橄榄(Elaeocarpus floribundus Blume)的花客多样性和授粉

IF 2.6 3区农林科学

Annals of Applied Biology Pub Date : 2022-10-11 DOI: 10.1111/aab.12808

Satyajit Oraon, Soumitra Pal, Subrata Mondal

引用次数: 1

Tomato chlorosis virus, a promiscuous virus with multiple host plants and whitefly vectors 番茄黄化病毒，一种具有多种寄主植物和粉虱载体的混杂病毒

IF 2.6 3区农林科学

Annals of Applied Biology Pub Date : 2022-10-09 DOI: 10.1111/aab.12809

Elvira Fiallo-Olivé, Jesús Navas-Castillo

引用次数: 3

Root cortical aerenchyma, characteristics and nutrient use efficiency of different maize hybrids during drought and rehydration process 干旱和复水过程中不同玉米杂交种的根皮质通气组织、特性及养分利用效率

IF 2.6 3区农林科学

Annals of Applied Biology Pub Date : 2022-10-06 DOI: 10.1111/aab.12807

Chen Hua, Mou Chengxiang, Attachai Jintrawet, Chen Jianfeng, Yin Mei, Fu Libo, Wang Wei, Zhiyuan Wang

{"title":"Root cortical aerenchyma, characteristics and nutrient use efficiency of different maize hybrids during drought and rehydration process","authors":"Chen Hua, Mou Chengxiang, Attachai Jintrawet, Chen Jianfeng, Yin Mei, Fu Libo, Wang Wei, Zhiyuan Wang","doi":"10.1111/aab.12807","DOIUrl":"10.1111/aab.12807","url":null,"abstract":"The importance of cortical aerenchyma in flood and drought resistance is well established. However, effects of the combination of the two factors remain unclear. We tested the hypothesis that under conditions of substantial water alteration, enhanced root cortical aerenchymas (RCAs) could not only exert a positive effect on root functions under drought periods but also influence nitrogen capture, soil exploration and compensation growth after rehydration. Three maize varieties, Yurui2 (YR2), Yunrui6 (YR6) and Yunrui167 (YR167), were grown under rainfed field conditions, and a subset was grown initially under well-watered and extreme-drought conditions, followed by the same simulated rainfed field conditions, in order to compare the effects on the RCAs during the growing season. Root morphology and function parameters were measured, and variation in the RCAs was induced by water stress. Under extreme drought, we found substantial variation in the RCAs (from 0% to 37%) and drought tolerance performance. During the rehydration stage, different RCA components exerted different effects on maize, recovery parameters and plant N use efficiency. YR6 showed resilient growth, whereas recovery was inhibited in YR167 and YR2, respectively, which possessed the lowest and highest RCA area percentages. The results support the hypothesis that RCA area percentage under drought periods are important for maize recovery after post-drought rehydration.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"182 2","pages":"192-204"},"PeriodicalIF":2.6,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48059354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light and storage time influence the microbial quality of minimally processed rocket 光照和储存时间对微加工火箭微生物质量的影响

IF 2.6 3区农林科学

Annals of Applied Biology Pub Date : 2022-09-26 DOI: 10.1111/aab.12806

Angela Zappia, Antonino Malacrinò, Imen Belgacem, Ahmed Abdelfattah, Amalia Piscopo

{"title":"Light and storage time influence the microbial quality of minimally processed rocket","authors":"Angela Zappia, Antonino Malacrinò, Imen Belgacem, Ahmed Abdelfattah, Amalia Piscopo","doi":"10.1111/aab.12806","DOIUrl":"10.1111/aab.12806","url":null,"abstract":"Ready-to-eat minimally processed fruits and vegetables are an ideal substrate for the growth of microorganisms, including human pathogens and mycotoxin-producing species, which question their quality and safety for customers. While we are aware of the importance of production workflows in structuring the products' microbial communities, we still know little about the factors that shape microbiomes during the timeframe products are available to customers, and beyond this timeframe. Here, we study the influence of storage light condition (light or dark) on microbiological and physico-chemical parameters of minimally processed rocket leaves at different shelf life timepoints (the day the product becomes available to consumers, expiration date, 3 days after the expiration date). Our results suggest that the total microbial load increases from the day the product becomes available to consumers, to the expiry date and after the product's expiration. However, when studying the composition of the fungal microbiome, we did not observe significant changes in its structure as the effect of product shelf life or storage light condition. We also found that products stored under light condition had a higher total bacterial load compared to those stored in darkness. Our results might be helpful in crafting improved workflows for product's storage during its shelf life, which might ultimately lead to a re-evaluation of storage times resulting in reduced food waste due to product spoilage or expiration.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"182 1","pages":"48-56"},"PeriodicalIF":2.6,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aab.12806","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42727421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ensemble learning integration of multiple CNN with improved vision transformer models for pest classification 用于害虫分类的多个CNN与改进的视觉变换器模型的集成学习

IF 2.6 3区农林科学

Annals of Applied Biology Pub Date : 2022-08-27 DOI: 10.1111/aab.12804

Wanshang Xia, Dezhi Han, Dun Li, Zhongdai Wu, Bing Han, Junxiang Wang

{"title":"An ensemble learning integration of multiple CNN with improved vision transformer models for pest classification","authors":"Wanshang Xia, Dezhi Han, Dun Li, Zhongdai Wu, Bing Han, Junxiang Wang","doi":"10.1111/aab.12804","DOIUrl":"10.1111/aab.12804","url":null,"abstract":"Pests are the main threats to crop growth, and the precision classification of pests is conducive to formulating effective prevention and governance strategies. In response to the problems of low efficiency and inadaptability to the large-scale environment of existing pest classification methods, this paper proposes a new pest classification method based on a convolutional neural network (CNN) and an improved Vision Transformer model. First, the MMAlNet is designed to extract the characteristics of the identification object from different scales and finer granularity. Then, a classification model called DenseNet Vision Transformer (DNVT) combining a CNN and an improved vision transformer model is proposed. The proposed DNVT captures both long distance dependencies and local characteristic modelling capabilities, which can effectively improve pest classification accuracy. Finally, the ensemble learning algorithm is used to learn MMAlNet and DNVT classification forecasts for soft voting, further enhancing the classification accuracy of pests. The simulation experiment results on the D0 and IP102 datasets show that the proposed method attained a maximum classification of 99.89 and 74.20%, respectively, which is better than other state-of-the-art methods and has a high practical application value.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"182 2","pages":"144-158"},"PeriodicalIF":2.6,"publicationDate":"2022-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45118275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Identification of four distinct ‘Candidatus Phytoplasma’ species in pomegranate trees showing witches' broom, little leaf and yellowing in Jordan, and preliminary insights on their putative insect vectors and reservoir plants 约旦石石树中4种不同的“候选植物”(Candidatus Phytoplasma)的鉴定，表现为“witch ' s broom”、“little leaf”和“泛黄”，并对其可能的传虫媒介和储集植物进行初步认识

IF 2.6 3区农林科学

Annals of Applied Biology Pub Date : 2022-08-24 DOI: 10.1111/aab.12803

Asem Habes Abu Alloush, Piero Attilio Bianco, Sadeer Amashah, Enrico Busato, Amre Mahasneh, Mahmoud AlShoubaki, Alberto Alma, Rosemarie Tedeschi, Fabio Quaglino

{"title":"Identification of four distinct ‘Candidatus Phytoplasma’ species in pomegranate trees showing witches' broom, little leaf and yellowing in Jordan, and preliminary insights on their putative insect vectors and reservoir plants","authors":"Asem Habes Abu Alloush, Piero Attilio Bianco, Sadeer Amashah, Enrico Busato, Amre Mahasneh, Mahmoud AlShoubaki, Alberto Alma, Rosemarie Tedeschi, Fabio Quaglino","doi":"10.1111/aab.12803","DOIUrl":"10.1111/aab.12803","url":null,"abstract":"During field surveys conducted in northern Jordan from June to November 2020, phytoplasma-like symptoms, including leaf yellowing/reddening and rolling, little leaf and witches' broom were observed in pomegranate. Disease incidence in 22 surveyed orchards ranged from 30% to 65%. Nested PCR-based amplification of 16S rRNA gene detected phytoplasmas in 17% of collected symptomatic pomegranate trees. Amplicon nucleotide sequence analyses allowed attributing the detected phytoplasmas to ‘Candidatus Phytoplasma solani’, ‘Ca. P. aurantifolia’, ‘Ca. P. asteris’ and ‘Ca. P. ulmi’. These phytoplasmas were found in plants showing specific symptoms and differentially distributed in the considered locations. Additionally, three cicadellids (Macrosteles sexnotatus, Cicadulina bipunctata and Psammotettix striatus) and two non-crop plants (Plantago major and Capsicum annuum) resulted hosting ‘Ca. P. asteris’ strains, and one cicadellid (Balclutha incisa) was carrying a ‘Ca. P. solani’ strain. A new pomegranate disease complex associated with multiple phytoplasmas, including ‘Ca. P. aurantifolia’ and ‘Ca. P. ulmi’, never reported before in this host plant, is described here. Moreover, preliminary indications are provided on its possible epidemiology in Jordan, involving two putative insect vectors (M. sexnotatus, B. incisa) first reported in the Country.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"182 2","pages":"159-170"},"PeriodicalIF":2.6,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48320650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of quality and nutritional traits from Lonicera caerulea (Honeyberry) cultivars and other berries grown in Scotland 金银花（Honeyberry）品种与苏格兰其他浆果品质和营养特性的比较分析

IF 2.6 3区农林科学

Annals of Applied Biology Pub Date : 2022-08-21 DOI: 10.1111/aab.12805

Louise Gamble, Simon D. A. Pont, J. William Allwood, Dorota A. Jarret, Robert D. Hancock

{"title":"Comparative analysis of quality and nutritional traits from Lonicera caerulea (Honeyberry) cultivars and other berries grown in Scotland","authors":"Louise Gamble, Simon D. A. Pont, J. William Allwood, Dorota A. Jarret, Robert D. Hancock","doi":"10.1111/aab.12805","DOIUrl":"10.1111/aab.12805","url":null,"abstract":"Lonicera caeruleabrix is a perennial shrub native to North America, Europe and Asia. It produces dark blue berries known as honeyberries or haskap berries which are produced commercially in several territories including Canada, Japan, Russia and Poland. Plants are suited to UK environments, but it is yet to be widely commercially developed in the UK. In the present work, quality and nutritional traits of six honeyberry cultivars grown in Scotland were compared with other commonly grown berry fruits (strawberry, raspberry, blueberry, blackberry, blackcurrant) to aid the identification of environmentally stable, high-quality honeyberry cultivars suitable for UK cultivation. Differences were observed in fruit quality variables (soluble solids, titratable acidity and Brix/acid ratios) between honeyberry cultivars. Three of six cultivars examined exhibited notable variation in soluble solids dependent on harvest year with ‘Aurora’ and ‘Strawberry Sensation’ having consistently high oBrix values. Titratable acidity exhibited cultivar differences and there was limited variation over harvest years. ‘Aurora’ exhibited consistently high oBrix/titratable acidity ratio reflected by high glucose and fructose content. Honeyberry fruit had good nutritional profile relative to other soft fruits with higher polyphenol and anthocyanin content than strawberry, blueberry, blackberry and raspberry, manifested in greater antioxidant capacity. The major anthocyanins in aqueous honeyberry fruit extracts were cyanidin, pelargonidin and peonidin glycosides. These findings indicate that L. caerulea represents a crop suitable for UK cultivation capable of producing high quality fruit with a valuable nutritional profile relative to other soft fruits. Cultivars exhibit significant differences in fruit quality and nutritional profile as well as harvest consistency and growers should consider this when establishing new plantations.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"182 2","pages":"171-182"},"PeriodicalIF":2.6,"publicationDate":"2022-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48812060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Exogenous salicylic acid alleviates water stress in watermelon plants 外源水杨酸缓解西瓜植株水分胁迫

IF 2.6 3区农林科学

Annals of Applied Biology Pub Date : 2022-08-09 DOI: 10.1111/aab.12802

Joanderson Marques Silva, Gabriel Barbosa da Silva Júnior, Aurenivia Bonifácio, Alexson Filgueiras Dutra, Renato de Mello Prado, Francisco de Alcântara Neto, Alan Mario Zuffo, Rafael Silva Melo, Tássyla Lohanne de Sousa Pereira, Ricardo Silva de Sousa

{"title":"Exogenous salicylic acid alleviates water stress in watermelon plants","authors":"Joanderson Marques Silva, Gabriel Barbosa da Silva Júnior, Aurenivia Bonifácio, Alexson Filgueiras Dutra, Renato de Mello Prado, Francisco de Alcântara Neto, Alan Mario Zuffo, Rafael Silva Melo, Tássyla Lohanne de Sousa Pereira, Ricardo Silva de Sousa","doi":"10.1111/aab.12802","DOIUrl":"10.1111/aab.12802","url":null,"abstract":"Salicylic acid (SA) has been considered to attenuate the effects of abiotic stresses on plants, including water deficit that intensely affects the growth and production of plants. The goal of this work was to evaluate the role of SA in the alleviation of water stress in watermelon seedlings on a morphophysiological and biochemical level. The experiment consisted of application of SA at concentrations of 0, 0.25, 0.50, 0.75 and 1.0 μmol L−1 to leaves of watermelon seedlings grown in three levels of water retention (100%, 75% and 50% WRL). To evaluate the effect on morphophysiological and biochemical aspects, the plant height, leaf area, shoot and root dry weight, chlorophyll index, relative water content, electrolyte leakage, protein content, amino acids, proline, carbohydrates, sucrose and starch concentration variables were determined. All variables were influenced by the SA concentrations and WRL, with statistically significant interaction between these factors for all except root dry weight. SA promotes increases in the concentration of organic solutes and reduces the rate of electrolyte leakage in watermelon seedlings, thus, supporting metabolism and growth of plants under stress conditions resulting from water deficit.","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"182 1","pages":"121-130"},"PeriodicalIF":2.6,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45696566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3