Zoonoses research最新文献_第5页

Construction and Evaluation of the Brucella Double Gene Knock-out Vaccine Strain MB6 Δbp26ΔwboA (RM6) 布鲁氏菌双基因敲除疫苗菌株MB6 Δbp26ΔwboA (RM6)的构建与评价

Zoonoses research Pub Date : 2022-09-14 DOI: 10.15212/zoonoses-2022-0031

Bochang Shi, Xinyu Li, Bo Li, Naxin Zheng, Min Li, Yuanzhan Liu, Chunhe Li, Fang Yan, Wei He, Liyan Zhao, Huabin Li, Chongyu Tian, Yuanqiang Zheng, Zhongpeng Zhao

引用次数: 1

Host Immune Responses to Arthritogenic Alphavirus Infection, with Emphasis on Type I IFN Responses 宿主对关节炎源性甲病毒感染的免疫反应，重点是I型干扰素反应

Zoonoses research Pub Date : 2022-09-09 DOI: 10.15212/zoonoses-2022-0028

Patrick Chun Hean Tang, Wern Hann Ng, Xiang Liu

引用次数: 2

Effectiveness of Inactivated SARS-CoV-2 Vaccines During a Delta Variant Outbreak in Hunan Province, China: A Retrospective Cohort Study SARS-CoV-2灭活疫苗在中国湖南省三角洲型疫情中的有效性:一项回顾性队列研究

Zoonoses research Pub Date : 2022-09-06 DOI: 10.15212/zoonoses-2022-0020

Xuemei Yan, Zhihui Dai, Qianhui Wu, Xiaole Wang, Yan Wang, G. Zeng, Yanpeng Wu, Shengbao Chen, L. Yi, Hongjie Yu, Lidong Gao

{"title":"Effectiveness of Inactivated SARS-CoV-2 Vaccines During a Delta Variant Outbreak in Hunan Province, China: A Retrospective Cohort Study","authors":"Xuemei Yan, Zhihui Dai, Qianhui Wu, Xiaole Wang, Yan Wang, G. Zeng, Yanpeng Wu, Shengbao Chen, L. Yi, Hongjie Yu, Lidong Gao","doi":"10.15212/zoonoses-2022-0020","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0020","url":null,"abstract":"\u0000\u0000This study was aimed at investigating the effectiveness of inactivated COVID-19 vaccines against the Delta variant.\u0000\u0000\u0000\u0000We performed a retrospective cohort study of close contacts of people with laboratory-confirmed SARS-CoV-2 infections in Hunan province, China, from July to August 2021. Mixed-effect logistic regression was used to estimate vaccine effectiveness (VE), and analyze the effects of the vaccination status of index cases and the exposure risk level on VE estimation.\u0000\u0000\u0000\u0000A total of 1,685 close contacts of 126 index cases were included; 835 (49.6%) had received two doses of inactivated vaccines, and the median interval between the 2nd dose and exposure was 48 days (IQR: 41 to 56 days). Full vaccination was defined as two doses at least 14 days before exposure. Adjusted VE estimates for full vaccination were 54.8% (95% CI: 7.7 to 77.9) and 68.4% (95% CI: 8.5 to 89.1) against symptomatic and moderate-to-severe COVID-19, respectively. VE for inactivated vaccines was difficult to observe if index cases had been fully vaccinated. The estimated VE with respect to infection protection was lower among household than non-household contacts.\u0000\u0000\u0000\u0000Complete primary immunization of two-dose inactivated COVID-19 vaccines protected against SARS-CoV-2 Delta variant infection. Infection risk was higher among vaccinated household contacts than vaccinated non-household contacts.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"77 5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88462652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic Manipulation Toolkits in Apicomplexan Parasites 顶复合体寄生虫的遗传操作工具包

Zoonoses research Pub Date : 2022-08-26 DOI: 10.15212/zoonoses-2022-0027

Zhipeng Niu, Lilan Xue, Xiaoyan Yin, B. Shen

{"title":"Genetic Manipulation Toolkits in Apicomplexan Parasites","authors":"Zhipeng Niu, Lilan Xue, Xiaoyan Yin, B. Shen","doi":"10.15212/zoonoses-2022-0027","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0027","url":null,"abstract":"Apicomplexan parasites are a group of intracellular pathogens of great medical and veterinary importance, including Toxoplasma gondii and Plasmodium, which cause toxoplasmosis and malaria, respectively. Efficient and accurate manipulation of their genomes is essential to dissect their complex biology and to design new interventions. Over the past several decades, scientists have continually optimized the methods for genetic engineering in these organisms, and tremendous progress has been made. Here, we review the genetic manipulation tools currently used in several apicomplexan parasites, and discuss their advantages and limitations. The widely used CRISPR/Cas9 genome editing technique has been adapted in several apicomplexans and shown promising efficiency. In contrast, conditional gene regulation is available in only a limited number of organisms, mainly Plasmodium and Toxoplasma, thus posing a research bottleneck for other parasites. Conditional gene regulation can be achieved with tools that regulate gene expression at the DNA, RNA or protein level. However, a universal tool to address all needs of conditional gene manipulation remains lacking. Understanding the scope of application is key to selecting the proper method for gene manipulation.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91038302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Lower Vitamin D Levels are Associated with Higher Seroprevalence of Toxoplasma gondii—a US National Survey Study 较低的维生素D水平与较高的弓形虫血清患病率相关——一项美国国家调查研究

Zoonoses research Pub Date : 2022-08-12 DOI: 10.15212/zoonoses-2022-0019

Jiaofeng Huang, Yinlian Wu, Mingfang Wang, Yueyong Zhu, Su Lin

{"title":"Lower Vitamin D Levels are Associated with Higher Seroprevalence of Toxoplasma gondii—a US National Survey Study","authors":"Jiaofeng Huang, Yinlian Wu, Mingfang Wang, Yueyong Zhu, Su Lin","doi":"10.15212/zoonoses-2022-0019","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0019","url":null,"abstract":"\u0000\u0000Vitamin D deficiency is associated with high susceptibility to infections. The present study aimed at exploring the relationship between vitamin D levels and Toxoplasma gondii (T. gondii) infection, on the basis of a nationally representative database.\u0000\u0000\u0000\u0000The study data came from the National Health and Nutrition Examination Surveys (NHANES) 2001–2004. Participants underwent both Toxoplasma IgG antibody testing and serum vitamin D testing. Vitamin D deficiency was defined by a serum 25-hydroxyvitamin D level <20 ng/mL. Multivariate logistic regression and propensity score matching were used to adjust for potential confounders. All analyses were conducted in R software.\u0000\u0000\u0000\u0000A total of 10613 participants were included. Among these, 3973 (37.4%) were vitamin D deficient, and 2070 (19.5%) were seropositive for T. gondii IgG antibody. Vitamin D deficiency was found in 42.3% of the seropositive population, compared with 36.3% of the seronegative population (P<0.001). After adjustment for sex, age, body mass index, smoking history, drinking history and testing season, vitamin D deficiency was associated with an elevated risk of T. gondii infection (OR=1.303, 95% CI=1.136–1.495, P<0.001). This effect persisted in the propensity matching cohort.\u0000\u0000\u0000\u0000Low vitamin D levels are associated with high seroprevalence of T. gondii.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89662129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

First detection of Rickettsia aeschlimannii in Hyalomma marginatum in Tibet, China 西藏边缘透明瘤首次检出艾氏立克次体

Zoonoses research Pub Date : 2022-08-09 DOI: 10.15212/zoonoses-2022-0026

Jun Jiao, Yonghui Yu, Peisheng He, Weiqiang Wan, Xuan Ouyang, B. Wen, Yi Sun, Xiaolu Xiong

{"title":"First detection of Rickettsia aeschlimannii in Hyalomma marginatum in Tibet, China","authors":"Jun Jiao, Yonghui Yu, Peisheng He, Weiqiang Wan, Xuan Ouyang, B. Wen, Yi Sun, Xiaolu Xiong","doi":"10.15212/zoonoses-2022-0026","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0026","url":null,"abstract":"\u0000\u0000\u0000Hyalomma marginatum is an important arthropod vector in the transmission of various zoonoses. The aim of this study was to identify the tick-borne pathogens (TBPs) maintained in Hy. marginatum in Tibet and to estimate the risk of human tick-borne diseases.\u0000\u0000\u0000\u0000Adult Hy. marginatum ticks (n = 14) feeding on yaks were collected. The individual DNA samples of these ticks were sequenced with metagenomic next-generation sequencing to survey the presence of TBPs. TBPs in individual ticks were identified with nested polymerase chain reaction (PCR) combined with DNA sequencing.\u0000\u0000\u0000\u0000The presence of Rickettsia, Anaplasma, and Ehrlichia in individual ticks was indicated by the taxonomic profiles at the genus level, but only Rickettsia aeschlimannii (100%, 13/13) was further detected in the ticks by nested PCR.\u0000\u0000\u0000\u0000This study provides information on the microbial communities of Hy. marginatum in Tibet, China, and provides the first report of R. aeschlimannii found in Hy. marginatum in Tibet. The results of this study indicated that yaks in Tibet are exposed to R. aeschlimannii.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81265176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genotype 5 Japanese Encephalitis Virus—Old Genotype, New Threat 基因型5日本脑炎病毒-老基因型，新威胁

Zoonoses research Pub Date : 2022-08-05 DOI: 10.15212/zoonoses-2022-0016

Weijia Zhang, Chongxiao Xu, K. Nie, Huanyu Wang

{"title":"Genotype 5 Japanese Encephalitis Virus—Old Genotype, New Threat","authors":"Weijia Zhang, Chongxiao Xu, K. Nie, Huanyu Wang","doi":"10.15212/zoonoses-2022-0016","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0016","url":null,"abstract":"Japanese encephalitis (JE) is an important viral encephalitis with epidemic status in Asia, which is caused by Japanese encephalitis virus (JEV), a member of the genus Flavivirus. JEV is divided into five genotypes. Genotype 5 (G5) is relatively neglected because of the limited number of cases and strains isolated. The first strain of G5 JEV (Muar strain) was isolated in Singapore in 1952 in a patient from Muar, Malaysia. The second strain (XZ0934) was isolated 57 years later in China, thus indicating the re-emergence of G5 JEV. A female patient who had been vaccinated against JE was infected with G5 JEV in Korea in 2015. JE is a vaccine-preventable disease, and its incidence has decreased with vaccination in many Asian countries. G3 JEV is the main candidate for current JE vaccines, which include attenuated, inactivated and chimeric type vaccines. However, the available vaccines do not provide adequate protection against the older G5 JEV lineage. Therefore, more research on this genotype is crucial for developing better detection methods, expanding surveillance to determine the possible chains of viral transmission for this new threat and developing a polyvalent JEV vaccine.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85935989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Susceptibilities of Yersinia pestis to Twelve Antimicrobial Agents in China 中国鼠疫耶尔森菌对12种抗菌药物的敏感性分析

Zoonoses research Pub Date : 2022-07-26 DOI: 10.15212/zoonoses-2022-0018

Jian He, Xiao-yan Yang, Juan Jin, Y. Xin, Yi-ting Wang, Sheng-jian Li, Qi Zhang, Yumeng Wang, Hai-lian Wu, J. Bai, Haoming Xiong, Yong Jin, Juan Li, Xuefei Zhang, Rui-xia Dai, Wei Li

{"title":"Susceptibilities of Yersinia pestis to Twelve Antimicrobial Agents in China","authors":"Jian He, Xiao-yan Yang, Juan Jin, Y. Xin, Yi-ting Wang, Sheng-jian Li, Qi Zhang, Yumeng Wang, Hai-lian Wu, J. Bai, Haoming Xiong, Yong Jin, Juan Li, Xuefei Zhang, Rui-xia Dai, Wei Li","doi":"10.15212/zoonoses-2022-0018","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0018","url":null,"abstract":"\u0000\u0000Streptomycin is the preferred choice for therapy of plague in China and other countries. However, Yersinia pestis exhibiting plasmid-mediated antimicrobial agent–resistant traits had been reported in Madagascar. In this study, we evaluated the susceptibility of traditional or newer antimicrobial agents used for treatment and/or prophylaxis of plague.\u0000\u0000\u0000\u0000Following Clinical and Laboratory Standards Institute (CLSI) recommendations, the susceptibility of 12 antimicrobial agents was evaluated by the agar microdilution method in 1,012 strains of Y. pestis isolated from 1943 to 2017 in 12 natural plague foci in China.\u0000\u0000\u0000\u0000One clinical Y. pestis isolate (S19960127) was found to be highly resistant to streptomycin, while the strain was still sensitive to other 11 antibiotics, that is, ciprofloxacin, ofloxacin, kanamycin, chloramphenicol, ampicillin, ceftriaxone, cefuroxime, trimethoprim-sulfamethoxazole, tetracycline, spectinomycin and moxifloxacin. The remaining 1,011 Y. pestis strains in this study demonstrated susceptibility to the above-mentioned 12 antimicrobial agents.\u0000\u0000\u0000\u0000Antimicrobial sensitivity surveillance of Y. pestis isolates, including dynamic monitoring of streptomycin resistance during various clinical plague treatments, should be carried out routinely.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88936452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Acute Severe Hepatitis of Unknown Etiology in Children 病因不明的儿童急性重型肝炎研究进展

Zoonoses research Pub Date : 2022-06-28 DOI: 10.15212/zoonoses-2022-0023

Xuefeng Zhou, Hongzhou Lu

引用次数: 1

The Novel Monkeypox Outbreak: What Should We Know and Reflect On? 新的猴痘爆发:我们应该知道和反思什么?

Zoonoses research Pub Date : 2022-06-28 DOI: 10.15212/zoonoses-2022-0022

Xiaoning Liu, Xiao Jiang, Zhenggang Zhu, Liqin Sun, Hongzhou Lu

引用次数: 1