Zoonoses research最新文献_第5页

The Role of Metagenomic Next-Generation Sequencing as a Promising Technology for Diagnosing HIV-TB Coinfection 新一代宏基因组测序在诊断HIV-TB合并感染中的作用

Zoonoses research Pub Date : 2022-10-04 DOI: 10.15212/zoonoses-2022-0032

Zhonghui Liao, Sumei Yang, Qian Li, Hongzhou Lu

{"title":"The Role of Metagenomic Next-Generation Sequencing as a Promising Technology for Diagnosing HIV-TB Coinfection","authors":"Zhonghui Liao, Sumei Yang, Qian Li, Hongzhou Lu","doi":"10.15212/zoonoses-2022-0032","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0032","url":null,"abstract":"The human immunodeficiency virus (HIV) pandemic has caused a resurgence of tuberculosis (TB), thus increasing morbidity and mortality. Moreover, HIV-TB coinfection leads to difficulties in diagnosis. Sputum smear microscopy, mycobacterial culture and GeneXpert MTB/RIF assays are generally endorsed to detect Mycobacterium tuberculosis (M. tuberculosis) in HIV-TB coinfection. However, these methods cannot diagnose TB in an accurate and timely manner, thus increasing the rates of HIV-associated morbidity and mortality in patients with TB. Hence, a considerable need exists for better diagnostic tools for patients with HIV-TB coinfection. Metagenomic next-generation sequencing (mNGS) is a novel detection platform widely used to assess infectious disease, antimicrobial resistance, the microbiome and human host gene expression. Herein, we summarize the advantages of mNGS for infectious disease diagnostics. We then assess the efficiency of mNGS in the detection of M. tuberculosis in different specimens and several cases of HIV-TB coinfection. We conclude that mNGS is an acceptable diagnostic method for HIV-TB coinfection, although limited research is available.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"743 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76894534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Distinct Genotype of Hantavirus Infection in Rodents in Jiangxi Province, China, in 2020–2021 2020-2021年江西省鼠类汉坦病毒感染差异基因型分析

Zoonoses research Pub Date : 2022-09-27 DOI: 10.15212/zoonoses-2022-0034

Xuefei Deng, Yun Xie, Shanshan Du, Xiaoxia Huang, Qin Wang, Aqian Li, Chuan Li, Sheng Ding, Li-na Sun, Wei Wu, Hao Li, Chun Xie, Xiaoqing Liu, Jian-dong Li

{"title":"Distinct Genotype of Hantavirus Infection in Rodents in Jiangxi Province, China, in 2020–2021","authors":"Xuefei Deng, Yun Xie, Shanshan Du, Xiaoxia Huang, Qin Wang, Aqian Li, Chuan Li, Sheng Ding, Li-na Sun, Wei Wu, Hao Li, Chun Xie, Xiaoqing Liu, Jian-dong Li","doi":"10.15212/zoonoses-2022-0034","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0034","url":null,"abstract":"\u0000\u0000Hantavirus causes hemorrhagic fever with renal syndrome, thus posing a major threat to human health in Jiangxi Province, China. Both Hantaan virus (HTNV) and Seoul virus (SEOV) have been found to be endemic in the province.\u0000\u0000\u0000\u0000Rodents were trapped from Gaoan Anyi and Tonggu counties in Jiangxi Province in 2020–2021. Hantavirus specific antibodies in the blood and RNA in the lung samples from the captured mammals were detected and analyzed.\u0000\u0000\u0000\u0000A total of 889 small mammals from seven species were collected. Positive detection was observed for hantavirus antibodies in 9.8% (87/889), SEOV RNA in 1% (9/889) and HTNV RNA in 2.6% (23/889). The difference in detection rates between regions was significant. Phylogenetic analysis of the obtained partial sequences of M and S segments revealed that two distinct genotypes of HTNV and three genotypes of SEOV were co-circulating in the captured mammals, with a regionally specific distribution.\u0000\u0000\u0000\u0000Multiple distinct genotypes of hantavirus are co-circulating in the province. Further studies in broader areas remain needed to reveal the diversity of hantaviruses.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80275386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evidence of the Efficacy and the Effectiveness of First Generation COVID-19 Vaccines in Clinical Trials and Real-world Studies 第一代COVID-19疫苗在临床试验和现实世界研究中的疗效和有效性证据

Zoonoses research Pub Date : 2022-09-21 DOI: 10.15212/zoonoses-2022-0025

Jing-xin Li, Zhuo-pei Li, Yawen Zhu, Li Zhou, F. Zhu

引用次数: 1

Construction and Evaluation of the Brucella Double Gene Knock-out Vaccine Strain MB6 Δbp26ΔwboA (RM6) 布鲁氏菌双基因敲除疫苗菌株MB6 Δbp26ΔwboA (RM6)的构建与评价

Zoonoses research Pub Date : 2022-09-14 DOI: 10.15212/zoonoses-2022-0031

Bochang Shi, Xinyu Li, Bo Li, Naxin Zheng, Min Li, Yuanzhan Liu, Chunhe Li, Fang Yan, Wei He, Liyan Zhao, Huabin Li, Chongyu Tian, Yuanqiang Zheng, Zhongpeng Zhao

引用次数: 1

Host Immune Responses to Arthritogenic Alphavirus Infection, with Emphasis on Type I IFN Responses 宿主对关节炎源性甲病毒感染的免疫反应，重点是I型干扰素反应

Zoonoses research Pub Date : 2022-09-09 DOI: 10.15212/zoonoses-2022-0028

Patrick Chun Hean Tang, Wern Hann Ng, Xiang Liu

引用次数: 2

Effectiveness of Inactivated SARS-CoV-2 Vaccines During a Delta Variant Outbreak in Hunan Province, China: A Retrospective Cohort Study SARS-CoV-2灭活疫苗在中国湖南省三角洲型疫情中的有效性:一项回顾性队列研究

Zoonoses research Pub Date : 2022-09-06 DOI: 10.15212/zoonoses-2022-0020

Xuemei Yan, Zhihui Dai, Qianhui Wu, Xiaole Wang, Yan Wang, G. Zeng, Yanpeng Wu, Shengbao Chen, L. Yi, Hongjie Yu, Lidong Gao

{"title":"Effectiveness of Inactivated SARS-CoV-2 Vaccines During a Delta Variant Outbreak in Hunan Province, China: A Retrospective Cohort Study","authors":"Xuemei Yan, Zhihui Dai, Qianhui Wu, Xiaole Wang, Yan Wang, G. Zeng, Yanpeng Wu, Shengbao Chen, L. Yi, Hongjie Yu, Lidong Gao","doi":"10.15212/zoonoses-2022-0020","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0020","url":null,"abstract":"\u0000\u0000This study was aimed at investigating the effectiveness of inactivated COVID-19 vaccines against the Delta variant.\u0000\u0000\u0000\u0000We performed a retrospective cohort study of close contacts of people with laboratory-confirmed SARS-CoV-2 infections in Hunan province, China, from July to August 2021. Mixed-effect logistic regression was used to estimate vaccine effectiveness (VE), and analyze the effects of the vaccination status of index cases and the exposure risk level on VE estimation.\u0000\u0000\u0000\u0000A total of 1,685 close contacts of 126 index cases were included; 835 (49.6%) had received two doses of inactivated vaccines, and the median interval between the 2nd dose and exposure was 48 days (IQR: 41 to 56 days). Full vaccination was defined as two doses at least 14 days before exposure. Adjusted VE estimates for full vaccination were 54.8% (95% CI: 7.7 to 77.9) and 68.4% (95% CI: 8.5 to 89.1) against symptomatic and moderate-to-severe COVID-19, respectively. VE for inactivated vaccines was difficult to observe if index cases had been fully vaccinated. The estimated VE with respect to infection protection was lower among household than non-household contacts.\u0000\u0000\u0000\u0000Complete primary immunization of two-dose inactivated COVID-19 vaccines protected against SARS-CoV-2 Delta variant infection. Infection risk was higher among vaccinated household contacts than vaccinated non-household contacts.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"77 5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88462652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic Manipulation Toolkits in Apicomplexan Parasites 顶复合体寄生虫的遗传操作工具包

Zoonoses research Pub Date : 2022-08-26 DOI: 10.15212/zoonoses-2022-0027

Zhipeng Niu, Lilan Xue, Xiaoyan Yin, B. Shen

{"title":"Genetic Manipulation Toolkits in Apicomplexan Parasites","authors":"Zhipeng Niu, Lilan Xue, Xiaoyan Yin, B. Shen","doi":"10.15212/zoonoses-2022-0027","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0027","url":null,"abstract":"Apicomplexan parasites are a group of intracellular pathogens of great medical and veterinary importance, including Toxoplasma gondii and Plasmodium, which cause toxoplasmosis and malaria, respectively. Efficient and accurate manipulation of their genomes is essential to dissect their complex biology and to design new interventions. Over the past several decades, scientists have continually optimized the methods for genetic engineering in these organisms, and tremendous progress has been made. Here, we review the genetic manipulation tools currently used in several apicomplexan parasites, and discuss their advantages and limitations. The widely used CRISPR/Cas9 genome editing technique has been adapted in several apicomplexans and shown promising efficiency. In contrast, conditional gene regulation is available in only a limited number of organisms, mainly Plasmodium and Toxoplasma, thus posing a research bottleneck for other parasites. Conditional gene regulation can be achieved with tools that regulate gene expression at the DNA, RNA or protein level. However, a universal tool to address all needs of conditional gene manipulation remains lacking. Understanding the scope of application is key to selecting the proper method for gene manipulation.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91038302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Lower Vitamin D Levels are Associated with Higher Seroprevalence of Toxoplasma gondii—a US National Survey Study 较低的维生素D水平与较高的弓形虫血清患病率相关——一项美国国家调查研究

Zoonoses research Pub Date : 2022-08-12 DOI: 10.15212/zoonoses-2022-0019

Jiaofeng Huang, Yinlian Wu, Mingfang Wang, Yueyong Zhu, Su Lin

{"title":"Lower Vitamin D Levels are Associated with Higher Seroprevalence of Toxoplasma gondii—a US National Survey Study","authors":"Jiaofeng Huang, Yinlian Wu, Mingfang Wang, Yueyong Zhu, Su Lin","doi":"10.15212/zoonoses-2022-0019","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0019","url":null,"abstract":"\u0000\u0000Vitamin D deficiency is associated with high susceptibility to infections. The present study aimed at exploring the relationship between vitamin D levels and Toxoplasma gondii (T. gondii) infection, on the basis of a nationally representative database.\u0000\u0000\u0000\u0000The study data came from the National Health and Nutrition Examination Surveys (NHANES) 2001–2004. Participants underwent both Toxoplasma IgG antibody testing and serum vitamin D testing. Vitamin D deficiency was defined by a serum 25-hydroxyvitamin D level <20 ng/mL. Multivariate logistic regression and propensity score matching were used to adjust for potential confounders. All analyses were conducted in R software.\u0000\u0000\u0000\u0000A total of 10613 participants were included. Among these, 3973 (37.4%) were vitamin D deficient, and 2070 (19.5%) were seropositive for T. gondii IgG antibody. Vitamin D deficiency was found in 42.3% of the seropositive population, compared with 36.3% of the seronegative population (P<0.001). After adjustment for sex, age, body mass index, smoking history, drinking history and testing season, vitamin D deficiency was associated with an elevated risk of T. gondii infection (OR=1.303, 95% CI=1.136–1.495, P<0.001). This effect persisted in the propensity matching cohort.\u0000\u0000\u0000\u0000Low vitamin D levels are associated with high seroprevalence of T. gondii.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89662129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

First detection of Rickettsia aeschlimannii in Hyalomma marginatum in Tibet, China 西藏边缘透明瘤首次检出艾氏立克次体

Zoonoses research Pub Date : 2022-08-09 DOI: 10.15212/zoonoses-2022-0026

Jun Jiao, Yonghui Yu, Peisheng He, Weiqiang Wan, Xuan Ouyang, B. Wen, Yi Sun, Xiaolu Xiong

{"title":"First detection of Rickettsia aeschlimannii in Hyalomma marginatum in Tibet, China","authors":"Jun Jiao, Yonghui Yu, Peisheng He, Weiqiang Wan, Xuan Ouyang, B. Wen, Yi Sun, Xiaolu Xiong","doi":"10.15212/zoonoses-2022-0026","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0026","url":null,"abstract":"\u0000\u0000\u0000Hyalomma marginatum is an important arthropod vector in the transmission of various zoonoses. The aim of this study was to identify the tick-borne pathogens (TBPs) maintained in Hy. marginatum in Tibet and to estimate the risk of human tick-borne diseases.\u0000\u0000\u0000\u0000Adult Hy. marginatum ticks (n = 14) feeding on yaks were collected. The individual DNA samples of these ticks were sequenced with metagenomic next-generation sequencing to survey the presence of TBPs. TBPs in individual ticks were identified with nested polymerase chain reaction (PCR) combined with DNA sequencing.\u0000\u0000\u0000\u0000The presence of Rickettsia, Anaplasma, and Ehrlichia in individual ticks was indicated by the taxonomic profiles at the genus level, but only Rickettsia aeschlimannii (100%, 13/13) was further detected in the ticks by nested PCR.\u0000\u0000\u0000\u0000This study provides information on the microbial communities of Hy. marginatum in Tibet, China, and provides the first report of R. aeschlimannii found in Hy. marginatum in Tibet. The results of this study indicated that yaks in Tibet are exposed to R. aeschlimannii.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81265176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genotype 5 Japanese Encephalitis Virus—Old Genotype, New Threat 基因型5日本脑炎病毒-老基因型，新威胁

Zoonoses research Pub Date : 2022-08-05 DOI: 10.15212/zoonoses-2022-0016

Weijia Zhang, Chongxiao Xu, K. Nie, Huanyu Wang

{"title":"Genotype 5 Japanese Encephalitis Virus—Old Genotype, New Threat","authors":"Weijia Zhang, Chongxiao Xu, K. Nie, Huanyu Wang","doi":"10.15212/zoonoses-2022-0016","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0016","url":null,"abstract":"Japanese encephalitis (JE) is an important viral encephalitis with epidemic status in Asia, which is caused by Japanese encephalitis virus (JEV), a member of the genus Flavivirus. JEV is divided into five genotypes. Genotype 5 (G5) is relatively neglected because of the limited number of cases and strains isolated. The first strain of G5 JEV (Muar strain) was isolated in Singapore in 1952 in a patient from Muar, Malaysia. The second strain (XZ0934) was isolated 57 years later in China, thus indicating the re-emergence of G5 JEV. A female patient who had been vaccinated against JE was infected with G5 JEV in Korea in 2015. JE is a vaccine-preventable disease, and its incidence has decreased with vaccination in many Asian countries. G3 JEV is the main candidate for current JE vaccines, which include attenuated, inactivated and chimeric type vaccines. However, the available vaccines do not provide adequate protection against the older G5 JEV lineage. Therefore, more research on this genotype is crucial for developing better detection methods, expanding surveillance to determine the possible chains of viral transmission for this new threat and developing a polyvalent JEV vaccine.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85935989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2