Zoonoses research最新文献_第4页

Development and Efficacy Evaluation of an SP01-adjuvanted Inactivated Escherichia Coli Mutant Vaccine Against Bovine Coliform Mastitis 牛大肠菌群乳腺炎sp01佐剂灭活大肠杆菌突变苗的研制及疗效评价

Zoonoses research Pub Date : 2022-12-09 DOI: 10.15212/zoonoses-2022-0039

Xinyu Li, Chongyu Tian, Hui Han, Bochang Shi, Huabin Li, Yuanzhan Liu, Wei He, Chunhe Li, Min Li, Yuanqiang Zheng, Bo Li, Liyan Zhao, Naxin Zheng, Fang Yan, Zhongpeng Zhao

{"title":"Development and Efficacy Evaluation of an SP01-adjuvanted Inactivated Escherichia Coli Mutant Vaccine Against Bovine Coliform Mastitis","authors":"Xinyu Li, Chongyu Tian, Hui Han, Bochang Shi, Huabin Li, Yuanzhan Liu, Wei He, Chunhe Li, Min Li, Yuanqiang Zheng, Bo Li, Liyan Zhao, Naxin Zheng, Fang Yan, Zhongpeng Zhao","doi":"10.15212/zoonoses-2022-0039","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0039","url":null,"abstract":"\u0000\u0000\u0000Escherichia coli (E. coli) is one of the most common pathogens causing clinical mastitis in cattle, but no vaccine is available to prevent this disease in China. Therefore, development of an E. coli vaccine against bovine clinical mastitis is urgently needed.\u0000\u0000\u0000\u0000The candidate vaccine (Ch-O111-1) and challenge (LZ06) strains were screened from milk samples of cows with clinical mastitis. To extend the cross-protection of the Ch-O111-1 strain, we deleted the galE gene fragment of the Ch-O111-1 strain through homologous recombination between the Ch-O111-1 strain and pCVD442/ΔgalE plasmid, which was identified through conventional methods, including PCR, SDS-PAGE and sequencing. The Ch-O111-1/ΔgalE (Z9) strain was characterized by extensive cross-reactivity and attenuated virulence. We prepared inactivated Z9 vaccines with different adjuvants.\u0000\u0000\u0000\u0000Immunization of inactivated Z9 antigen induced adjuvant-, dosage- and inoculation time-dependent antibody titers in cows and mice. Furthermore, immunization with SP01-adjuvanted inactivated Z9 vaccine protected cows against severe clinical mastitis caused by LZ06 and protected mice against death due to LZ06.\u0000\u0000\u0000\u0000An SP01-adjuvanted inactivated Z9 vaccine was successfully developed and found to protect cows against severe mastitis caused by Escherichia coli.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82345809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Detection of Candidatus Coxiella mudorwiae in Haemaphysalis concinna in China 中国大陆血蜱中莫氏考希氏候选菌的分子检测

Zoonoses research Pub Date : 2022-12-07 DOI: 10.15212/zoonoses-2022-0041

Mingjie Shi, Tong Qin, Zhitong Liu, Hao Feng, Yi Sun

{"title":"Molecular Detection of Candidatus Coxiella mudorwiae in Haemaphysalis concinna in China","authors":"Mingjie Shi, Tong Qin, Zhitong Liu, Hao Feng, Yi Sun","doi":"10.15212/zoonoses-2022-0041","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0041","url":null,"abstract":"\u0000\u0000\u0000Coxiella burnetii and Coxiella-like endosymbionts (CLEs) have been widely discovered in various ticks, animals, and even human beings. To estimate the possible origin of C. burnetii and its relatives CLEs, the prevalence of C. burnetii and CLEs has been intensively surveyed all over the world.\u0000\u0000\u0000\u0000In the present study, the possible infection of C. burnetii and CLEs in host-seeking Haemaphysalis concinna was performed with meta-transcript analysis with tick specimens harvested from Mudanjiang City, Heilongjiang province, China. The meta-transcript results were subsequently confirmed by the specific sequence of partial 16S rRNA.\u0000\u0000\u0000\u0000A total of three arrays of gene transcripts were harvested, including pyrophosphate-fructose 6-phosphate 1-phosphotransferase-eda-thiol-disulfide isomerase and thioredoxin-greA, carB-carA-DnaJ-DnaK-grpE-ppnk, ropC-ropB, and ubiA-non-canonical purine NTP pyrophosphatase-hemK-prfA, which suggest the infection of Candidatus Coxiella mudorwiae in H. concinna. The high identity of the 16S rRNA gene of Candidatus C. mudorwiae achieved in our study strongly supports our meta-transcripts analysis.\u0000\u0000\u0000\u0000The prevalence of Candidatus C. mudorwiae in hard ticks has been discovered in China. More detailed surveys are imperative to clarify the emergence of CLEs and their implication in the epidemiologic characteristics of Q fever.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72756229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Infectious Diseases in October 2022: Monthly Analysis 2022年10月全球传染病:月度分析

Zoonoses research Pub Date : 2022-12-07 DOI: 10.15212/zoonoses-2022-1003

Yi Luo, Yinfu Sun, Wenjin Yu, Jiazhen Zou, Guodan Li, Taihan Li, Qi Xiang, Dongliang Liu, Shiping He, Qun Su, D. Gu

引用次数: 0

One Health Approach for the Control of Zoonotic Diseases 控制人畜共患疾病的一种健康途径

Zoonoses research Pub Date : 2022-11-30 DOI: 10.15212/zoonoses-2022-0037

Gashaw Adane Erkyihun, Meseret Bekele Alemayehu

{"title":"One Health Approach for the Control of Zoonotic Diseases","authors":"Gashaw Adane Erkyihun, Meseret Bekele Alemayehu","doi":"10.15212/zoonoses-2022-0037","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0037","url":null,"abstract":"Zoonoses are infectious diseases that can be transmitted from animals to humans and/or from humans to animals. Approximately 75% of new emerging and re-emerging disease pathogens are zoonotic: 60% spread from domestic and wild animals, and 80% are of concern regarding bioterrorism. For the past 20 years, the world has faced several zoonotic disease outbreaks. Some zoonoses can cause recurring outbreaks, such as Ebola virus disease, salmonellosis, Marburg disease, rabies and anthrax. Still others, such as the novel coronavirus causing COVID-19, have the potential to cause worldwide pandemics. The high risk of emerging and reemerging disease spillover and burden has been increased by extensive interactions among animals, humans and ecosystems. Hence, this complex health threat strongly requires a multi-sectoral collaboration known as the One Health approach. This article discusses recent scientific policies, initiatives, best practices, systems and challenges in One Health. The critical rapid review method was used, with a particular emphasis on available and recent global information. Literature found in Google Scholar and PubMed, and data publicly available on the websites of major global, regional and national organizations were included. The aim of this article is to provide compiled information and increase awareness.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"201 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76407553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Microbial Communities in the Lungs of Bats in China 中国蝙蝠肺部微生物群落

Zoonoses research Pub Date : 2022-11-25 DOI: 10.15212/zoonoses-2022-0033

Na Zhao, H. Ren, Yan Jiang, Yinan Li, Xingxing Lian, T. Qin

{"title":"Microbial Communities in the Lungs of Bats in China","authors":"Na Zhao, H. Ren, Yan Jiang, Yinan Li, Xingxing Lian, T. Qin","doi":"10.15212/zoonoses-2022-0033","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0033","url":null,"abstract":"\u0000\u0000Bats are the hosts of multiple pathogens, but the microbial composition of their lung tissues remains unknown. Our study investigated the species compositions and genera of important respiratory tract pathogenic bacteria in bat lung tissue.\u0000\u0000\u0000\u0000A microbiota study was conducted in Hebei, Henan and Guizhou provinces in China. Lung tissues were collected from 104 healthy bats. The lung tissue was subjected to 16S ribosomal ribonucleic acid gene sequencing.\u0000\u0000\u0000\u0000We obtained 7,708,734 high-quality bacterial sequences from 104 healthy bats. Overall, the annotations indicated 55 phyla, 73 classes, 164 orders, 322 families and 953 genera. The lung microbiota was highly polymorphic and variable among bats from Hebei, Henan and Guizhou. The genetic characteristics of the main recognized respiratory pathogens in the samples were analyzed.\u0000\u0000\u0000\u0000The findings indicate that the lungs of bats carry numerous bacteria with pathogenic importance. Pathogens disseminate through the respiratory tract in bats and are widely distributed among bats. Because bats prefer to inhabit areas placing them in close contact with humans, such as eaves and old buildings, further investigations are warranted to identify bat microbiota and their potential effects on humans.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"285 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86165127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Infectious Diseases in September 2022: Monthly Analysis 2022年9月全球传染病:月度分析

Zoonoses research Pub Date : 2022-11-15 DOI: 10.15212/zoonoses-2022-1002

Guodan Li, Dongliang Liu, Wenjin Yu, Qi Xiang, Jiazhen Zou, Taihan Li, Yi Luo, Shiping He, Qun Su, D. Gu

引用次数: 1

Global Infectious Diseases in August of 2022: Monthly Analysis 2022年8月全球传染病:月度分析

Zoonoses research Pub Date : 2022-10-07 DOI: 10.15212/zoonoses-2022-1001

Qi Xiang, Taihan Li, Jiazhen Zou, Guodan Li, Yi Luo, Shiping He, D. Gu

引用次数: 1

The Role of Metagenomic Next-Generation Sequencing as a Promising Technology for Diagnosing HIV-TB Coinfection 新一代宏基因组测序在诊断HIV-TB合并感染中的作用

Zoonoses research Pub Date : 2022-10-04 DOI: 10.15212/zoonoses-2022-0032

Zhonghui Liao, Sumei Yang, Qian Li, Hongzhou Lu

{"title":"The Role of Metagenomic Next-Generation Sequencing as a Promising Technology for Diagnosing HIV-TB Coinfection","authors":"Zhonghui Liao, Sumei Yang, Qian Li, Hongzhou Lu","doi":"10.15212/zoonoses-2022-0032","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0032","url":null,"abstract":"The human immunodeficiency virus (HIV) pandemic has caused a resurgence of tuberculosis (TB), thus increasing morbidity and mortality. Moreover, HIV-TB coinfection leads to difficulties in diagnosis. Sputum smear microscopy, mycobacterial culture and GeneXpert MTB/RIF assays are generally endorsed to detect Mycobacterium tuberculosis (M. tuberculosis) in HIV-TB coinfection. However, these methods cannot diagnose TB in an accurate and timely manner, thus increasing the rates of HIV-associated morbidity and mortality in patients with TB. Hence, a considerable need exists for better diagnostic tools for patients with HIV-TB coinfection. Metagenomic next-generation sequencing (mNGS) is a novel detection platform widely used to assess infectious disease, antimicrobial resistance, the microbiome and human host gene expression. Herein, we summarize the advantages of mNGS for infectious disease diagnostics. We then assess the efficiency of mNGS in the detection of M. tuberculosis in different specimens and several cases of HIV-TB coinfection. We conclude that mNGS is an acceptable diagnostic method for HIV-TB coinfection, although limited research is available.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"743 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76894534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Distinct Genotype of Hantavirus Infection in Rodents in Jiangxi Province, China, in 2020–2021 2020-2021年江西省鼠类汉坦病毒感染差异基因型分析

Zoonoses research Pub Date : 2022-09-27 DOI: 10.15212/zoonoses-2022-0034

Xuefei Deng, Yun Xie, Shanshan Du, Xiaoxia Huang, Qin Wang, Aqian Li, Chuan Li, Sheng Ding, Li-na Sun, Wei Wu, Hao Li, Chun Xie, Xiaoqing Liu, Jian-dong Li

{"title":"Distinct Genotype of Hantavirus Infection in Rodents in Jiangxi Province, China, in 2020–2021","authors":"Xuefei Deng, Yun Xie, Shanshan Du, Xiaoxia Huang, Qin Wang, Aqian Li, Chuan Li, Sheng Ding, Li-na Sun, Wei Wu, Hao Li, Chun Xie, Xiaoqing Liu, Jian-dong Li","doi":"10.15212/zoonoses-2022-0034","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0034","url":null,"abstract":"\u0000\u0000Hantavirus causes hemorrhagic fever with renal syndrome, thus posing a major threat to human health in Jiangxi Province, China. Both Hantaan virus (HTNV) and Seoul virus (SEOV) have been found to be endemic in the province.\u0000\u0000\u0000\u0000Rodents were trapped from Gaoan Anyi and Tonggu counties in Jiangxi Province in 2020–2021. Hantavirus specific antibodies in the blood and RNA in the lung samples from the captured mammals were detected and analyzed.\u0000\u0000\u0000\u0000A total of 889 small mammals from seven species were collected. Positive detection was observed for hantavirus antibodies in 9.8% (87/889), SEOV RNA in 1% (9/889) and HTNV RNA in 2.6% (23/889). The difference in detection rates between regions was significant. Phylogenetic analysis of the obtained partial sequences of M and S segments revealed that two distinct genotypes of HTNV and three genotypes of SEOV were co-circulating in the captured mammals, with a regionally specific distribution.\u0000\u0000\u0000\u0000Multiple distinct genotypes of hantavirus are co-circulating in the province. Further studies in broader areas remain needed to reveal the diversity of hantaviruses.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80275386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evidence of the Efficacy and the Effectiveness of First Generation COVID-19 Vaccines in Clinical Trials and Real-world Studies 第一代COVID-19疫苗在临床试验和现实世界研究中的疗效和有效性证据

Zoonoses research Pub Date : 2022-09-21 DOI: 10.15212/zoonoses-2022-0025

Jing-xin Li, Zhuo-pei Li, Yawen Zhu, Li Zhou, F. Zhu

引用次数: 1