新一代宏基因组测序在诊断HIV-TB合并感染中的作用

Zhonghui Liao, Sumei Yang, Qian Li, Hongzhou Lu
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引用次数: 2

摘要

人类免疫缺陷病毒(HIV)的流行导致结核病(TB)的死灰复燃,从而增加了发病率和死亡率。此外,艾滋病毒-结核病合并感染导致诊断困难。痰涂片镜检、分枝杆菌培养和GeneXpert MTB/RIF检测通常被认可用于检测HIV-TB合并感染中的结核分枝杆菌(M. tuberculosis)。然而,这些方法不能准确和及时地诊断结核病,从而增加了结核病患者中与艾滋病毒相关的发病率和死亡率。因此,迫切需要为艾滋病毒-结核病合并感染患者提供更好的诊断工具。新一代宏基因组测序(metagenomics next-generation sequencing, mNGS)是一种新型的检测平台,广泛用于评估传染病、抗菌素耐药性、微生物组和人类宿主基因表达。在此,我们总结了mNGS在传染病诊断中的优势。然后,我们评估了mNGS在不同标本和几种HIV-TB合并感染病例中检测结核分枝杆菌的效率。我们得出结论,尽管现有研究有限,但mNGS是一种可接受的HIV-TB合并感染的诊断方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Role of Metagenomic Next-Generation Sequencing as a Promising Technology for Diagnosing HIV-TB Coinfection
The human immunodeficiency virus (HIV) pandemic has caused a resurgence of tuberculosis (TB), thus increasing morbidity and mortality. Moreover, HIV-TB coinfection leads to difficulties in diagnosis. Sputum smear microscopy, mycobacterial culture and GeneXpert MTB/RIF assays are generally endorsed to detect Mycobacterium tuberculosis (M. tuberculosis) in HIV-TB coinfection. However, these methods cannot diagnose TB in an accurate and timely manner, thus increasing the rates of HIV-associated morbidity and mortality in patients with TB. Hence, a considerable need exists for better diagnostic tools for patients with HIV-TB coinfection. Metagenomic next-generation sequencing (mNGS) is a novel detection platform widely used to assess infectious disease, antimicrobial resistance, the microbiome and human host gene expression. Herein, we summarize the advantages of mNGS for infectious disease diagnostics. We then assess the efficiency of mNGS in the detection of M. tuberculosis in different specimens and several cases of HIV-TB coinfection. We conclude that mNGS is an acceptable diagnostic method for HIV-TB coinfection, although limited research is available.
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