Zoonoses research最新文献_第3页

Visual and Rapid Detection of Escherichia coli O157:H7 in Stool Samples by FTA Card-based Loop-mediated Isothermal Amplification 基于FTA卡环介导等温扩增的粪便样品中大肠杆菌O157:H7的视觉快速检测

Zoonoses research Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0022

Fumin Chen, Junyu Wang, Weiguang Li, Yuqian Zhang, Leshan Xiu, Qinqin Hu, Zhengshang Ruan, Ping Chen, Kun Yin

{"title":"Visual and Rapid Detection of Escherichia coli O157:H7 in Stool Samples by FTA Card-based Loop-mediated Isothermal Amplification","authors":"Fumin Chen, Junyu Wang, Weiguang Li, Yuqian Zhang, Leshan Xiu, Qinqin Hu, Zhengshang Ruan, Ping Chen, Kun Yin","doi":"10.15212/zoonoses-2023-0022","DOIUrl":"https://doi.org/10.15212/zoonoses-2023-0022","url":null,"abstract":"Escherichia coli O157:H7 (E. coli O157:H7) can induce severe diseases in animals and humans that result in significant public health problems. Therefore, the development of rapid and visual detection methods to diagnose E. coli O157:H7 infections and monitor its prevalence is critical for the prevention and control purposes. A colorimetric loop-mediated isothermal amplification (LAMP) assay was utilized to detect E. coli O157:H7. A DNA extraction kit and Flinders Technology Associates (FTA) cards were used to extract nucleic acid in conjunction with colorimetric LAMP detection. The method developed effectively distinguished E. coli O157:H7 from other pathogens with a detection limit of 25 CFU/mL in spiked stool samples. In addition, the nucleic acid of these samples was easily extracted and transported with an FTA card at room temperature. The entire detection process was completed within 35 min using simple constant-temperature equipment. The colorimetric LAMP method with FTA card-based nucleic acid purification was shown to rapidly detect E. coli O157:H7 with sensitivity and specificity. This visual method is expected to be widely used to control E. coli O157:H7 infections, particularly in resource-limited settings.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72838061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiologic Features of Human Rabies in China from 2015-2021 2015-2021年中国人狂犬病流行病学特征分析

Zoonoses research Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0012

Na Zhang, Chengqian Song, X. Tao, Wu Zhu

{"title":"Epidemiologic Features of Human Rabies in China from 2015-2021","authors":"Na Zhang, Chengqian Song, X. Tao, Wu Zhu","doi":"10.15212/zoonoses-2023-0012","DOIUrl":"https://doi.org/10.15212/zoonoses-2023-0012","url":null,"abstract":"This study aimed to enhance the current understanding of the epidemiologic characteristics, laboratory diagnostic levels, and changes in pathogenic populations of rabies in China by studying the status of the human rabies epidemic in China from 2015-2021 and provide useful information for guiding rabies disease prevention and control strategies. We analyzed the incidence, distribution, and laboratory testing of human rabies in mainland China using statutory surveillance data from 2015-2021. Based on a literature review, the study summarizes the recent updates of the rabies virus population in each province based on previous monitoring. A total of 3032 rabies cases were reported in China from 2015-2021, with a year-after-year decrease in the total number of cases. Most of the cases (75.19%) were distributed in Hunan, Henan, Guangxi, Guizhou, Hubei, Yunnan, Jiangsu, Anhui, Guangdong, and Sichuan, with 13 counties (districts) reporting > 50 cases in 7 years. The number of reported counties (districts) decreased from 512 in 2015 to 116 in 2021. Farmers accounted for most of the cases (73%), and the highest proportion of cases (54.62%) occurred in individuals 50-75 years of age. No changes in endemic populations were detected in China. The laboratory diagnosis rate of cases increased from 4.74% in 2015 to 22.93% in 2021. The rabies epidemic in China decreased steadily from 2015-2021, with a marked contraction in the geographic scope. In the future it will be necessary to continue to carry out large-scale dog immunization and strengthen the surveillance and laboratory diagnosis of rabies.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72698987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Infectious Diseases in June 2023: Monthly Analysis 2023年6月全球传染病:月度分析

Zoonoses research Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-1007

Shuqiong Zhang, Xin Fu, Shiping He, Jiazhen Zou, Yi Luo, Guodan Li, Qi Xiang, Taihan Li, D. Gu, Qun Su, Minjing He, Wenjin Yu

引用次数: 0

Zoonotic Enteric Parasites among Pastoralists, Cattle, and Soil in the Upper Benue Trough of Northeastern Nigeria 尼日利亚东北部上贝努埃海槽牧民、牛和土壤中的人畜共患肠道寄生虫

Zoonoses research Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2022-0051

Sani Njobdi, O. Akogun, M. Ja’afaru

{"title":"Zoonotic Enteric Parasites among Pastoralists, Cattle, and Soil in the Upper Benue Trough of Northeastern Nigeria","authors":"Sani Njobdi, O. Akogun, M. Ja’afaru","doi":"10.15212/zoonoses-2022-0051","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0051","url":null,"abstract":"The occupation, lifestyle, and lack of formal education among pastoralists place them at higher risk of zoonoses. Moreover, zoonoses among pastoralists and their livestock in the Upper Benue Trough in northeastern Nigeria has not been studied holistically. Therefore, we investigated zoonotic enteric parasite (ZEP) infections by Entamoba spp., Cryptosporidium spp., Giardia intestinalis, Fasciola spp., Taenia spp. and Trichostrongylus spp. among this group. Demographic information and faecal samples were collected from humans and cattle in 12 pastoral communities along the trough using a cross-sectional, observational study design. Soil samples were also collected from homes. Specimens were examined microscopically for ZEPs and the data were analysed. The prevalence of ZEPs was 40.3% among humans, 48.2% among cattle, and 74.6% in home soil. The prevalence of ZEP infections among humans did not differ significantly with respect to gender and husbandry practices, but did differ significantly with respect to age and clan. There was a strong correlation (R=0.750) between ZEP prevalence in humans, cattle, and soil across study communities. The correlation between the distribution of ZEPs in different sample categories across communities strongly suggests that zoonotic transmission of ZEP is ongoing in the study area. Adopting an integrated approach to intervention will potentially be more effective in disease control. Further investigation, continuous monitoring, and surveillance are recommended to forestall enteric infection outbreaks.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"132 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73479302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring the Process and Characterizing Symptoms of Suckling Mouse Inoculation Promote Isolating Viruses from Ticks 乳鼠接种过程监测及症状表征促进蜱病毒分离

Zoonoses research Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0023

Shuang Tang, Sijing Hu, Jian Xiao, Yan-fang Zhang, Zhèngyuān Sū, Qiaoli Wu, Juan Yang, Xijia Liu, Zhong Zhang, Qiong Zhu, Junming Shi, Abilimiti Moming, Yali Deng, Z. Fan, R. Guo, Sùróng Sūn, Yujiang Zhang, S. Shěn, Fei Deng

{"title":"Monitoring the Process and Characterizing Symptoms of Suckling Mouse Inoculation Promote Isolating Viruses from Ticks","authors":"Shuang Tang, Sijing Hu, Jian Xiao, Yan-fang Zhang, Zhèngyuān Sū, Qiaoli Wu, Juan Yang, Xijia Liu, Zhong Zhang, Qiong Zhu, Junming Shi, Abilimiti Moming, Yali Deng, Z. Fan, R. Guo, Sùróng Sūn, Yujiang Zhang, S. Shěn, Fei Deng","doi":"10.15212/zoonoses-2023-0023","DOIUrl":"https://doi.org/10.15212/zoonoses-2023-0023","url":null,"abstract":"Suckling mouse inoculation is an important method that has been used for years to isolate viruses from ticks; however, this method has usually been briefly described in the literature on a case-by-case basis upon successful isolation rather than providing extensive details. This study describes the procedure from preparation of tick homogenates to identification of virus isolation using the suckling mouse inoculation method. The transient and persistent features were characterized and the incidence of manifestations that developed in the suckling mice, especially in mice from which viruses were isolated, is reported. We identified 22 symptoms that developed in mice, including 13 transient symptoms that recovered by the end of the observation period and 7 persistent symptoms that the mice suffered from throughout the observation period. Persistent symptoms (lateral positioning and dead) and transient symptoms (malaise, emaciation, and difficulty turning over) were the main symptoms based on the high overall incidence. Moreover, we showed that mice from which viruses were isolated had a concentrated period and advanced days of disease onset. This study provides detailed information necessary for better use of suckling mouse inoculation to isolate viruses from ticks, which may benefit optimization of this method to identify, discover, and acquire tick-borne viruses.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85573089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of Anaplasma, Ehrlichia, Babesia, and Borrelia in Ticks from Guangdong Province of Southern China 广东省蜱类无形体、埃利希体、巴贝斯虫和伯氏体的分子特征分析

Zoonoses research Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0017

Li Zhang, Zhengkai Wei, Li Ding, Jiaming Hou, Jianhui Zhang, Bo Zhang, Jun Ma, Mingxin Song, QUAN LIU

{"title":"Molecular Characterization of Anaplasma, Ehrlichia, Babesia, and Borrelia in Ticks from Guangdong Province of Southern China","authors":"Li Zhang, Zhengkai Wei, Li Ding, Jiaming Hou, Jianhui Zhang, Bo Zhang, Jun Ma, Mingxin Song, QUAN LIU","doi":"10.15212/zoonoses-2023-0017","DOIUrl":"https://doi.org/10.15212/zoonoses-2023-0017","url":null,"abstract":"Tick-borne pathogens have become emerging global health threats in humans and animals. The objective of the present study was to investigate Anaplasma, Ehrlichia, Babesia, and Borrelia in ticks in southern China. Ticks were collected from animals and identified to the species level. Tick-borne Anaplasma, Ehrlichia, Babesia, and Borrelia were tested by PCR assays, and analyzed by sequencing and phylogenetics. We collected 747 ticks, including Rhipicephalus microplus (n=225) and R. sanguineus (n=522), from pet dogs in Guangdong Province of southern China. Using PCR and phylogenetic analysis we showed that these ticks carried Anaplasma platys, Ehrlichia canis, Borrelia miyamotoi, Babesia vogeli, and an unclassified Ehrlichia species in Rhipicephalus ticks. Of these pathogens, B. miyamotoi was first found in southern China. A. platys, E. canis, and B. miyamotoi were zoonotic pathogens that infected both R. microplus and R. sanguineus, whereas Ba. vogeli only infected R. sanguineus, with a prevalence of 0.3%-1.5%. The results of this study showed diversity and complexity of tick-borne pathogens in Guangdong Province of southern China, implying the importance of tick-borne pathogen surveillance and control.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"100 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79502192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Resistance, Virulence, and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Recovered from Ready-to-Eat (RTE) Food in China: A New Challenge for Food Safety 中国即食食品中耐甲氧西林金黄色葡萄球菌的耐药性、毒力和遗传特征:食品安全的新挑战

Zoonoses research Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0025

W. Wang, Hui Li, Changwei Wang, Fengqin Li, Y. Dong, Jing Xiao

{"title":"Antimicrobial Resistance, Virulence, and Genetic Characterization of Methicillin-Resistant Staphylococcus aureus Recovered from Ready-to-Eat (RTE) Food in China: A New Challenge for Food Safety","authors":"W. Wang, Hui Li, Changwei Wang, Fengqin Li, Y. Dong, Jing Xiao","doi":"10.15212/zoonoses-2023-0025","DOIUrl":"https://doi.org/10.15212/zoonoses-2023-0025","url":null,"abstract":"The objective of the present study was to determine the prevalence, antimicrobial resistance, virulence profiles, and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) obtained from ready-to-eat (RTE) foods in China. Two hundred seventy-six RTE food-associated S. aureus isolates were collected from 25 provinces across China in 2018, then characterized by antimicrobial susceptibility testing, virulence factors detecting, multilocus sequence typing (MLST), spa typing, SCCmec typing and pulsed-field gel electrophoresis (PFGE). Two hundred fifty isolates (90.6%) were resistant to at least one antimicrobial agent; 73 (26.4%) isolates were multi-drug resistant (MDR). Thirty MRSA isolates were identified, among which nine toxin genes (sea, seb, sec, sed, seh, selk, sell, selq, and tsst-1) were detected. Sixty percent (18/30) of the MRSA isolates harbored multiple toxin genes. Four virulence gene patterns were identified, with seb-selk-selq (30/30) being the most common pattern. Thirteen sequence types, as well as 13 spa and 4 SCCmec types were found among 30 MRSA isolates. The most prevalent MRSA lineages were CC59-t437-SCCmecIV/V (23.3% [7/30]), CC398-t011-SCCmecV (23.3% [7/30]), and CC1-t114-SCCmecIV (16.7% [5/30]). Our findings highlight the importance for the identification of prevalent clones, assessment of drug-resistance and virulence, and formulation of food safety measures for public health.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77969771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Infectious Diseases in July 2023: Monthly Analysis 2023年7月全球传染病:月度分析

Zoonoses research Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-1008

Guodan Li, Ying Zhou, Yinfu Sun, Qi Xiang, Tingting Jiang, Jiazhen Zou, Yufan Wu, Taihan Li, Yi Luo, Minjing He, Shuqiong Zhang, D. Gu, Shiping He, Dongliang Liu, Wenjin Yu, Jing Xie

引用次数: 0

High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, China 云南五种蜱类相关微生物群的高度多样性

Zoonoses research Pub Date : 2023-01-01 DOI: 10.15212/zoonoses-2023-0005

J. Zhang, Xue-bing Ni, Zijie Chen, Yun-Huan Zhang, Ran Wei, Z. Gong, D. Zhu, Xiao-ming Cui, Zong-ti Shao, Hong-Bo Liu, Yu-qiong Li, Jian Wang, Zihou Gao, X. Duan, Hang Jiang, Jia-Fu Jiang, T. Lam, En-jiong Huang, Na Jia, C. Du

{"title":"High Diversity of Tick-associated Microbiota from Five Tick Species in Yunnan, China","authors":"J. Zhang, Xue-bing Ni, Zijie Chen, Yun-Huan Zhang, Ran Wei, Z. Gong, D. Zhu, Xiao-ming Cui, Zong-ti Shao, Hong-Bo Liu, Yu-qiong Li, Jian Wang, Zihou Gao, X. Duan, Hang Jiang, Jia-Fu Jiang, T. Lam, En-jiong Huang, Na Jia, C. Du","doi":"10.15212/zoonoses-2023-0005","DOIUrl":"https://doi.org/10.15212/zoonoses-2023-0005","url":null,"abstract":"Ticks are obligate blood-sucking vectors for multiple zoonotic diseases. In this study, tick samples were collected from Yunnan Province, China, which is well-known as the “Global Biodiversity Hotspot” in the world. This study aimed to clarify the microbial populations, including pathogens, associated with ticks and to identify the diversity of tick-borne microbiota in this region. The 16S rRNA full-length sequencing from pooled tick DNA samples and PCR amplification of pathogenic genera from individual samples were performed to understand tick-associated microbiota in this region. A total of 191 adult ticks of 5 tick species were included and revealed 11 phyla and 126 genera bacteria, including pathogenic Anaplasma, Ehrlichia, Candidatus Neoehrlichia, Rickettsia, Borrelia, and Babesia. Further identification suggested that Rickettsia sp. YN01 was a variant strain of Rickettsia spp. IG-1, but Rickettsia sp. YN02 and Rickettsia sp. YN03, were potentially two new SFGR species. This study revealed the complexity of ecological interactions between host and microbe and provided insight for the biological control of ticks. A high microbial diversity in ticks from Yunnan was identified, and more investigation should be undertaken to elucidate the pathogenicity in the area.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83967412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Infectious Diseases in November 2022: Monthly Analysis 2022年11月全球传染病:月度分析

Zoonoses research Pub Date : 2022-12-28 DOI: 10.15212/zoonoses-2022-1004

Jiazhen Zou, Shiping He, Wenjin Yu, Guodan Li, Taihan Li, Qi Xiang, Dongliang Liu, Yi Luo, Yinfu Sun, Minjing He, Shuqiong Zhang, Qun Su, D. Gu

引用次数: 0