Zoonoses research最新文献_第6页

Susceptibilities of Yersinia pestis to Twelve Antimicrobial Agents in China 中国鼠疫耶尔森菌对12种抗菌药物的敏感性分析

Zoonoses research Pub Date : 2022-07-26 DOI: 10.15212/zoonoses-2022-0018

Jian He, Xiao-yan Yang, Juan Jin, Y. Xin, Yi-ting Wang, Sheng-jian Li, Qi Zhang, Yumeng Wang, Hai-lian Wu, J. Bai, Haoming Xiong, Yong Jin, Juan Li, Xuefei Zhang, Rui-xia Dai, Wei Li

{"title":"Susceptibilities of Yersinia pestis to Twelve Antimicrobial Agents in China","authors":"Jian He, Xiao-yan Yang, Juan Jin, Y. Xin, Yi-ting Wang, Sheng-jian Li, Qi Zhang, Yumeng Wang, Hai-lian Wu, J. Bai, Haoming Xiong, Yong Jin, Juan Li, Xuefei Zhang, Rui-xia Dai, Wei Li","doi":"10.15212/zoonoses-2022-0018","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0018","url":null,"abstract":"\u0000\u0000Streptomycin is the preferred choice for therapy of plague in China and other countries. However, Yersinia pestis exhibiting plasmid-mediated antimicrobial agent–resistant traits had been reported in Madagascar. In this study, we evaluated the susceptibility of traditional or newer antimicrobial agents used for treatment and/or prophylaxis of plague.\u0000\u0000\u0000\u0000Following Clinical and Laboratory Standards Institute (CLSI) recommendations, the susceptibility of 12 antimicrobial agents was evaluated by the agar microdilution method in 1,012 strains of Y. pestis isolated from 1943 to 2017 in 12 natural plague foci in China.\u0000\u0000\u0000\u0000One clinical Y. pestis isolate (S19960127) was found to be highly resistant to streptomycin, while the strain was still sensitive to other 11 antibiotics, that is, ciprofloxacin, ofloxacin, kanamycin, chloramphenicol, ampicillin, ceftriaxone, cefuroxime, trimethoprim-sulfamethoxazole, tetracycline, spectinomycin and moxifloxacin. The remaining 1,011 Y. pestis strains in this study demonstrated susceptibility to the above-mentioned 12 antimicrobial agents.\u0000\u0000\u0000\u0000Antimicrobial sensitivity surveillance of Y. pestis isolates, including dynamic monitoring of streptomycin resistance during various clinical plague treatments, should be carried out routinely.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88936452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in Acute Severe Hepatitis of Unknown Etiology in Children 病因不明的儿童急性重型肝炎研究进展

Zoonoses research Pub Date : 2022-06-28 DOI: 10.15212/zoonoses-2022-0023

Xuefeng Zhou, Hongzhou Lu

引用次数: 1

The Novel Monkeypox Outbreak: What Should We Know and Reflect On? 新的猴痘爆发:我们应该知道和反思什么?

Zoonoses research Pub Date : 2022-06-28 DOI: 10.15212/zoonoses-2022-0022

Xiaoning Liu, Xiao Jiang, Zhenggang Zhu, Liqin Sun, Hongzhou Lu

引用次数: 1

Identification of N-formylated Peptides with Neutrophilic Chemotactic Activity in Mycobacterium tuberculosis 结核分枝杆菌中具有中性粒细胞趋化活性的n -甲酰化肽的鉴定

Zoonoses research Pub Date : 2022-05-31 DOI: 10.15212/zoonoses-2022-0013

K. Dean, Bock-Gie Jung, Josimar Dornelas-Moreira, B. Samten

{"title":"Identification of N-formylated Peptides with Neutrophilic Chemotactic Activity in Mycobacterium tuberculosis","authors":"K. Dean, Bock-Gie Jung, Josimar Dornelas-Moreira, B. Samten","doi":"10.15212/zoonoses-2022-0013","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0013","url":null,"abstract":"Neutrophil infiltration of the lungs is associated with granuloma formation and the severity of tuberculosis infection. Although several cytokines and chemokines are known to contribute to lung neutrophil infiltration, the neutrophilic chemotactic factors of Mycobacterium tuberculosis (Mtb) remain unexplored. Therefore, we performed Transwell based chemotactic assays using neutrophils from human peripheral blood and mouse bone marrow to probe the chemotactic activity of the culture filtrates (CF) of Mtb H37Rv. CF of H37Rv induced chemotaxis of both human and mouse neutrophils, and this was also confirmed with CF of 9 clinical isolates and Erdman strain of Mtb with neutrophil chemotactic activity. Sulfasalazine, an N-formyl-Met-Leu-Phe (fMLF) receptor inhibitor, blocked the chemotaxis of neutrophils induced by CF of Mtb, thus indicating the involvement of the fMLF receptor in Mtb CF induced chemotaxis of neutrophils. Mass spectrometry analysis of CF of H37Rv identified three candidate N-formylated heptapeptides. The chemotactic activity of the identified peptides was confirmed with their synthetic mimetics that they induced neutrophil chemotaxis in a manner dependent on N-terminal formylation. For all formylated peptides and CF of Mtb, the induced Ca2+ influx in neutrophils was suppressed by sulfasalazine. Thus, we identified novel formylated Mtb peptides with neutrophil chemotactic activity.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83590330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test 重组酶聚合酶扩增联合侧流试验快速检测致病性猪链球菌2型

Zoonoses research Pub Date : 2022-05-27 DOI: 10.15212/zoonoses-2022-0015

Yong Qi, Wei Li, Xiaoling Li, Wanpeng Shen, Rui-Chen Lv, Nianhong Lu, Jiameng Li, Susu Zhuang, Yingjia Xu, Qiyuan Gui, Hongbing Shen, Yuexi Li

{"title":"Rapid Visual Detection of Pathogenic Streptococcus suis Type 2 through a Recombinase Polymerase Amplification Assay Coupled with Lateral Flow Test","authors":"Yong Qi, Wei Li, Xiaoling Li, Wanpeng Shen, Rui-Chen Lv, Nianhong Lu, Jiameng Li, Susu Zhuang, Yingjia Xu, Qiyuan Gui, Hongbing Shen, Yuexi Li","doi":"10.15212/zoonoses-2022-0015","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0015","url":null,"abstract":"\u0000\u0000\u0000Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen causing serious disease and even death in pigs and humans. Public health events and economic losses caused by SS2 have prompted widespread concern. Because of the unavailability of vaccines, the development of rapid detection methods for timely diagnosis of SS2 infection or contaminated products, and monitoring of its prevalence in susceptible animals and populations, is required to aid in the prevention and control of SS2 infections.\u0000\u0000\u0000\u0000Several sets of primers and one probe for a recombinase polymerase amplification (RPA) assay targeting the cpsJ2 gene were designed and synthesized. Lateral flow (LF) tests in combination with RPA were used to provide visual results. Primers with high amplification efficiency were screened, and the reaction system was optimized. Indicators of detection effectiveness were evaluated.\u0000\u0000\u0000\u0000The established method had a detection limit of 100 copies/reaction for recognizing SS2 rather than other organisms. The sensitivity was 100%, as evaluated in infected animal samples. The detection could be completed within 20 min and required only constant temperature equipment.\u0000\u0000\u0000\u0000The established rapid, visual, sensitive and specific RPA-LF assay showed superior detection performance and is expected to be widely applied to fight SS2 infection in resource-limited areas.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83286980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Zoonotic Cryptosporidium Parasites Possess a Unique Carbohydrate-binding Protein (Malectin) that is Absent in other Apicomplexan Lineages 人畜共患隐孢子虫寄生虫具有独特的碳水化合物结合蛋白(Malectin)，这在其他顶复合体谱系中是不存在的

Zoonoses research Pub Date : 2022-05-10 DOI: 10.15212/zoonoses-2022-0011

Mingbo Wu, Bo Yang, Dongqiang Wang, Ying Zhang, Xiaohui Li, Yubo Zhi, Xinhui Zhao, Jigang Yin, G. Zhu

{"title":"Zoonotic Cryptosporidium Parasites Possess a Unique Carbohydrate-binding Protein (Malectin) that is Absent in other Apicomplexan Lineages","authors":"Mingbo Wu, Bo Yang, Dongqiang Wang, Ying Zhang, Xiaohui Li, Yubo Zhi, Xinhui Zhao, Jigang Yin, G. Zhu","doi":"10.15212/zoonoses-2022-0011","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0011","url":null,"abstract":"\u0000\u0000Malectin is a carbohydrate-binding protein that binds Glc(2)-N-glycan and is present in animals and some alveolates. This study aimed to characterize the general molecular and biochemical features of Cryptosporidium parvum malectin (CpMal).\u0000\u0000\u0000\u0000Polyclonal antibodies were raised for detecting native CpMal by western blotting and immunofluorescence assays. Recombinant CpMal and human malectin (HsMal) were produced, and their binding activities to amylose and the host cell surface were compared. Far-western blotting and far-immunofluorescence assays were used to detect potential binding partners of CpMal in the parasite.\u0000\u0000\u0000\u0000Native CpMal appeared to exist in dimeric form in the parasite and was distributed in a diffuse pattern over sporozoites but was highly concentrated on the anterior and posterior sides near the nuclei. CpMal, compared with HsMal, had significantly lower affinity for binding amylose but substantially higher activity for binding host cells. Recombinant CpMal recognized three high molecular weight protein bands and labeled the sporozoite posterior end corresponding to the crystalloid body, thus suggesting the presence of its potential ligands in the parasite. Two proteins identified by proteomics should be prioritized for future validation of CpMal-binding.\u0000\u0000\u0000\u0000CpMal notably differs from HsMal in molecular and biochemical properties; thus, further investigation of its biochemical and biological roles is warranted.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82923130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genome Characterization and Phylogenetic Analysis of Bovine Hepacivirus in Inner Mongolia, Northeastern China 内蒙古牛肝炎病毒基因组特征及系统发育分析

Zoonoses research Pub Date : 2022-05-10 DOI: 10.15212/zoonoses-2022-0003

Zi-Jiang Liu, Liang Li, Yu Guo, Wenbo Xu, Yongxu Yuan, X. Liang, Wei Wang, Yinghua Zhao, Liyan Sui, Xian-min Feng, F. Wei, QUAN LIU, Zedong Wang

{"title":"Genome Characterization and Phylogenetic Analysis of Bovine Hepacivirus in Inner Mongolia, Northeastern China","authors":"Zi-Jiang Liu, Liang Li, Yu Guo, Wenbo Xu, Yongxu Yuan, X. Liang, Wei Wang, Yinghua Zhao, Liyan Sui, Xian-min Feng, F. Wei, QUAN LIU, Zedong Wang","doi":"10.15212/zoonoses-2022-0003","DOIUrl":"https://doi.org/10.15212/zoonoses-2022-0003","url":null,"abstract":"\u0000\u0000Bovine hepacivirus (BovHepV) is a new member of the genus Hepacivirus in the family Flaviviridae, which has been detected in cattle in more than seven countries. The purpose of this study was to identify and genetically characterize BovHepV in cattle in Inner Mongolia, northeastern (NE) China.\u0000\u0000\u0000\u0000A total of 116 serum samples from cattle were collected from HulunBuir in Inner Mongolia from April to May, 2021, and were divided into three pools for metagenomic sequencing. The samples were verified with semi-nested RT-PCR with primers based on the BovHepV sequences obtained from metagenomic sequencing. The complete genomes of BovHepV were amplified, and were used for genome characterization and phylogenetic analysis.\u0000\u0000\u0000\u0000BovHepV was detected in two pools through metagenomic sequencing. Five BovHepV positive samples were identified in Yakeshi of HulunBuir, thus indicating a prevalence of 8.8% (5/57). Two 8840 nucleotide long BovHepV strains YKS01/02 were amplified from the positive samples and showed 79.3%–91.9% nucleotide sequence identity with the discovered BovHepV strains. Phylogenetic analysis classified the YKS01/02 strains into BovHepV subtype G group.\u0000\u0000\u0000\u0000This study reports the first identification of BovHepV in cattle in northeastern China, and expands the known geographical distribution and genetic diversity of BovHepV in the country.\u0000","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87139627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Animal Origin of Major Human Infectious Diseases: What Can Past Epidemics Teach Us About Preventing the Next Pandemic? 人类主要传染病的动物起源:过去的流行病对预防下一次大流行有什么启示?

Zoonoses research Pub Date : 2022-04-01 DOI: 10.15212/zoonoses-2021-0028

G. Dharmarajan, Ruiyun Li, E. Chanda, Katharine R. Dean, R. Dirzo, K. Jakobsen, Imroze Khan, H. Leirs, Z. Shí, N. Wolfe, Ruifu Yang, N. Stenseth

{"title":"The Animal Origin of Major Human Infectious Diseases: What Can Past Epidemics Teach Us About Preventing the Next Pandemic?","authors":"G. Dharmarajan, Ruiyun Li, E. Chanda, Katharine R. Dean, R. Dirzo, K. Jakobsen, Imroze Khan, H. Leirs, Z. Shí, N. Wolfe, Ruifu Yang, N. Stenseth","doi":"10.15212/zoonoses-2021-0028","DOIUrl":"https://doi.org/10.15212/zoonoses-2021-0028","url":null,"abstract":"Emerging infectious diseases are one of the greatest public health challenges. Approximately three-quarters of these diseases are of animal origin. These diseases include classical zoonoses maintained in humans only via transmission from other vertebrates (e.g., rabies) and those initiated by a successful one-off zoonotic event (host-switch) in conjunction with efficient human-to-human transmission (e.g., H1N1 influenza). Here, we provide a systematic review, in conjunction with a meta-analysis and spatial risk modeling, to identify the major characteristics of past epidemics of animal origin and predict areas with high future disease emergence risk. Countermeasures against future pandemics of animal origin must focus on several key mechanisms. First, the eco-epidemiological contexts favoring spillover events must be clearly establish. Second, pathogen surveillance must be scaled up, particularly in taxa and/or eco-geographic areas with high disease emergence risk. Third, successful spillover risk must be mitigated through proactive strategies to interrupt animal-to-human transmission chains. Fourth, to decrease epidemic potential and prevent epidemics from becoming pandemics, improved source identification and real-time spatial tracking of diseases are crucial. Finally, because pandemics do not respect international borders, enhancing international collaboration is critical to improving preparedness and response.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73087949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Recombinase Polymerase Amplification for Rapid Detection of Zoonotic Pathogens: An Overview 重组酶聚合酶扩增用于人畜共患病原体的快速检测:综述

Zoonoses research Pub Date : 2022-03-25 DOI: 10.15212/zoonoses-2022-0002

Ruicheng Lv, Nianhong Lu, Junhu Wang, Yuexi Li, Yong Qi

引用次数: 3

Advances in Spectral Techniques for Detection of Pathogenic Microorganisms 病原微生物光谱检测技术研究进展

Zoonoses research Pub Date : 2022-03-18 DOI: 10.15212/zoonoses-2021-0027

Zixuan Xin, Jiating Chen, Hongjuan Peng

{"title":"Advances in Spectral Techniques for Detection of Pathogenic Microorganisms","authors":"Zixuan Xin, Jiating Chen, Hongjuan Peng","doi":"10.15212/zoonoses-2021-0027","DOIUrl":"https://doi.org/10.15212/zoonoses-2021-0027","url":null,"abstract":"The highly contagious viral illness Coronavirus disease 2019, caused by severe acute respiratory syndrome coronavirus-2, has led to nearly 5 million deaths worldwide. The detection of highly infectious pathogens or novel pathogens causing emerging infectious diseases is highly challenging. Encouragingly, spectral detection—including laser-induced fluorescence spectroscopy, infrared absorption spectroscopy, Raman spectroscopy and their combinations—has been broadly used to detect pathogenic microorganisms on the basis of their physical and chemical characteristics. Surface-enhanced Raman spectroscopy with labels can detect organisms at a minimum concentration of 3 cells/mL. The changes in cells’ biochemical reactions before and after polioviral infection can be detected by Fourier transform infrared spectroscopy. However, the sensitivity and specificity of different spectral detection categories differs, owing to their different detection principles. Flexible detection methods require interdisciplinary researchers familiar with both pathogen biology and instruments. This review summarizes the advances in spectral techniques used in detecting pathogenic microorganism.","PeriodicalId":79199,"journal":{"name":"Zoonoses research","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91292352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1