Animal Feed Science and Technology最新文献

{"title":"Changes in performance, apparent ileal digestibility and intestinal morphology of broiler chickens fed diets containing sunflower hulls with full-fat canola seed","authors":"Ramin Kasaeizadeh ,&nbsp;Somayyeh Salari ,&nbsp;Mohammad Reza Abdollahi ,&nbsp;Farshad Baghban","doi":"10.1016/j.anifeedsci.2025.116417","DOIUrl":"10.1016/j.anifeedsci.2025.116417","url":null,"abstract":"<div><div>The effect of addition of sunflower hulls (SFH) as an insoluble fiber on performance and some physiological parameters of broiler chickens fed diets containing full-fat canola seed (CS) was evaluated from 11 to 42 d of age in a 2 × 2 factorial arrangement of treatments. A total of 240 one-day-old broilers (Ross 308) were randomly devoted to 4 treatments, with 6 replicates each and 10 birds per replicate. Treatments were canola oil source (CS, and canola meal plus oil) and sunflower hulls levels (0 and 30 g/kg). During the 11–24 d of age (Grower period), canola oil source and SFH levels had no (P &gt; 0.05) significant interactions on performance. The 30 g/kg SFH inclusion and canola oil resulted in higher body weight gain (BWG) and lower feed conversion ratio (FCR) compared to those fed without SFH and CS diets (P &lt; 0.05). During the 25–42 d of age (Finisher period), in the CS diets, the SFH inclusion reduced (P &lt; 0.05) FCR compared to the diet without SFH. Over the whole trial period, broilers receiving SFH had better (P &lt; 0.05) performance compared to those fed without SFH. Feeding diets with canola oil reduced feed intake (FI) and improved FCR when compared to CS. The inclusion of SFH resulted in a heavier relative gizzard weight than diets without SFH in the canola oil diets, whereas had no effect on this organ in CS diets, resulting in a CS × SFH level interaction (P &lt; 0.05). The inclusion of SFH increased (P &lt; 0.05) ileal organic matter (OM) and crude protein (CP) digestibility in the canola oil diets. In both diets, the inclusion of SFH increased (P &lt; 0.05) the numbers of the <em>Lactobacillus</em> spp. in comparison with diets without SFH, but this effect was more pronounced in canola oil than CS diets. In the CS diets, feeding SFH resulted in lower (P &lt; 0.05) numbers of <em>Escherichia coli</em> in comparison with diets without SFH, while in canola oil diets, it was not influenced. Birds fed SFH showed a greater (P &lt; 0.05) duodenal villus height (VH) and jejunal VH and epithelial thickness (ET) than those fed without SFH. Overall, the current results suggest that whole CS, if supplemented with SFH, can be used in broiler diets without negative effect on the growth performance with beneficial effects on intestinal morphology. Inclusion of canola oil in broiler diets with SFH can benefit nutrient digestibility.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"327 ","pages":"Article 116417"},"PeriodicalIF":2.5,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144261576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral-stomach sampling as an alternative to rumen canula for the inoculation of in vitro batch fermentation systems","authors":"Vincent Niderkorn ,&nbsp;Sandra Point ,&nbsp;Angelique Torrent ,&nbsp;Pierre Nozière ,&nbsp;Mathieu Silberberg ,&nbsp;Anne Boudon","doi":"10.1016/j.anifeedsci.2025.116416","DOIUrl":"10.1016/j.anifeedsci.2025.116416","url":null,"abstract":"<div><div>The standard method to collect rumen fluid (RF) serving as inoculum for <em>in vitro</em> rumen fermentation assays and using ruminally cannulated animals is less and less accepted in some countries, and oral-stomach sampling (OSS) could be an alternative that needs to be validated. The objective of this study was to compare the <em>in vitro</em> rumen fermentation parameters of a large set of substrates with contrasted fermentation profiles using inocula obtained by OSS and from different sampling sites in the rumen of cannulated dairy cows. Rumen fermentation assays were conducted using twelve different substrates (six forages and six total mixed rations) and three types of inoculum consisting in fresh RF sampled in the reticulum (RF_ret), sampled both in the reticulum and the ventral sac (RF_mix) and using OSS (RF_tub) during three feeding periods creating variability in RF composition (control and acidogenic diets). There was a strong effect of substrate on all the parameters (P &lt; 0.001) and a limited effect of the type of RF on the overall fermentability of substrates, particularly between RF_mix (standard method) and RF_tub that had similar values for dry matter (DM) and fiber degradabilities, gas production (including proportion of methane (CH₄) in the gas produced), total volatile fatty acids (VFA) and ammonia (NH₃). Above all, we did not detect any interaction between the substrate and the type of RF, highlighting the possibility to measure confidently relative differences among substrates or treatments. Overall, our results showed that fresh OSS can be a relevant alternative to the fresh RF sampling using cannulated animals. To keep OSS as a research tool on the long term, efforts should be continued to improve the standardization and the refinement of the OSS method.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"327 ","pages":"Article 116416"},"PeriodicalIF":2.5,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal levels of methionine and the impact of water leaching in juvenile Penaeus vannamei diets","authors":"Felipe N. Façanha ,&nbsp;Karthik Masagounder ,&nbsp;Alberto J.P. Nunes","doi":"10.1016/j.anifeedsci.2025.116406","DOIUrl":"10.1016/j.anifeedsci.2025.116406","url":null,"abstract":"<div><div>An 8-week feeding trial was conducted to determine the optimal levels of dietary methionine (Met) for juvenile Pacific whiteleg shrimp, <em>Penaeus vannamei</em>, while evaluating the effects of amino acid (AA) leaching. Six practical diets were formulated to be isonitrogenous and isolipidic, with total Met levels ranging from 0.51 % to 1.37 % in 0.14–0.21 % increments, and total sulfur AA (Met + Cysteine) levels from 0.88 % to 1.75 % on a dry matter basis (DM). The total nitrogen content was balanced across all diets by replacing Met with a non-essential AA mixture. Each diet was randomly assigned to seven replicate groups of 15 juvenile shrimp with an initial body weight (BW) of 0.80 ± 0.05 g. Shrimp were fed six times daily until apparent satiation. The effects of varying dietary Met levels on survival, growth performance, feed utilization, and muscle tissue composition were evaluated, with Met leaching rates measured at 30 minutes in full-strength seawater. Results showed consistently high survival rates across all treatments, while final shrimp BW significantly increased with higher Met levels, particularly between diets containing 0.82 % and 0.99 % Met. Met leaching rates positively correlated with synthetic Met supplementation, significantly increasing as total dietary Met concentration rose. Muscle tissue composition analysis highlighted Met's crucial role in protein synthesis and physiological function, revealing significant differences in crude protein and essential AA deposition among dietary treatments. Higher dietary Met promoted optimal protein and AA muscle content up to 0.82 %, after which a saturation effect was observed. In conclusion, this study emphasizes the importance of accounting for nutrient leaching when determining optimal AA levels for juvenile <em>P. vannamei</em>. Quadratic regression analysis indicated that an optimal dietary Met level of approximately 1.04 % in the dry diet (2.73 % of dietary protein) was most effective under these experimental conditions. Considering Met leaching rates to more accurately estimate the amount of Met available to shrimp after feed exposure to water, it was established that an optimal level of 0.74 % Met in the dry diet (2.20 % of dietary protein) after 30 minutes of immersion in water optimally supports growth and feed efficiency. This finding refines the actual optimal dietary Met level for juvenile <em>Penaeus vannamei</em>, establishing a new benchmark for future nutritional research in shrimp aquaculture.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"327 ","pages":"Article 116406"},"PeriodicalIF":2.5,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144211972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding laying hens with insect meal affects the production traits and some quality parameters of table eggs","authors":"Károly Dublecz ,&nbsp;Henrik Baranyay ,&nbsp;Nikoletta Such ,&nbsp;Fabian Weinlaender ,&nbsp;Julian Kern ,&nbsp;Kesete Goitom Tewelde ,&nbsp;László Pál ,&nbsp;Brigitta Kiss ,&nbsp;László Wágner ,&nbsp;Tivadar Csiszér","doi":"10.1016/j.anifeedsci.2025.116415","DOIUrl":"10.1016/j.anifeedsci.2025.116415","url":null,"abstract":"<div><div>A feeding trial was carried out with Tetra SL laying hens to evaluate the effects of an insect larvae meal on the production traits and egg quality parameters. <em>Alphitobius diaperinus</em> larvae meal (ADM) was incorporated at 10 % (ADM10) and 15 % (ADM15) into layer diets on the expense of soybean meal and the production traits and egg quality parameters, have been evaluated. Among the production traits only egg weight was affected by dietary treatments. Both ADM diets decreased egg weight in comparison with the control diet. Besides egg weight, none of the other egg quality parameters were affected. ADM failed to modify the fat content of egg yolk but resulted significant changes in the fatty acid composition of the yolk fat. Feeding ADM increased the lauric, stearic and linoleic acid and decreased the oleic and α-linolenic acid concentrations of the yolk. The results of the electronic nose proved that ADM resulted in specific volatile compound structure, that can be used for the identification of the insect meal eggs. Among the identified compounds ADM reduced the intensity of dimethyl sulphide, ethyl acetate and acetaldehyde in comparison with the control eggs. The tasting and organoleptic evaluation of boiled eggs by the volunteers resulted higher quality scores and acceptance of the ADM eggs. From the results it can be concluded that insect meals can be used efficiently even at high incorporation rates in layer diets. The reason for the egg weight reduction, however, needs further investigation.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"327 ","pages":"Article 116415"},"PeriodicalIF":2.5,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing sustainable production efficiency in rainbow trout (Oncorhynchus mykiss) through feed restriction strategies under conditions of industrial production","authors":"Rosendo L. Azcuy ,&nbsp;Matías E. Casaretto ,&nbsp;Adrián J. Hernández ,&nbsp;Lorenzo Márquez ,&nbsp;Francisco J. Moyano ,&nbsp;Gabriel A. Morales","doi":"10.1016/j.anifeedsci.2025.116402","DOIUrl":"10.1016/j.anifeedsci.2025.116402","url":null,"abstract":"<div><div>This study aimed to investigate the impact of different lengths of feed restriction pulses on compensatory growth, nutrient utilization, body compositions and organ indices of rainbow trout (<em>Oncorhynchus mykiss</em>) during a five-week assay. Four feeding regimes were implemented: continuous feeding near-satiation, cycles of two days per week of fasting, one week feed restriction at maintenance level and re-feeding, and one week feed deprivation and re-feeding. These regimes were applied to triplicate groups of 2000 fish with an initial average weight and length of 66.4 ± 0.4 g and 17.2 ± 0.1 cm, respectively. Fish were allocated among 12 production-scale cages (80 m³) submerged in water at 15.2 ± 1.5 ºC. One week restriction or deprivation affected body condition factor, visceral index, lipid reserves and lipid energy/protein energy ratio without altering body protein levels. However, these values were restored to the levels of continuously fed fish after three weeks of refeeding, indicating partial compensatory responses in both restricted fish groups. Fish subjected to fasting cycles of two days per week exhibited not differences from continuously fed fish in growth parameters and whole-body composition, indicating a full compensatory response. Additionally, cycles of two days per week of fasting demonstrated lower weekly feed consumption (74.8 ± 1.5 vs. 87.9 ± 2.1 g fish⁻¹), improved overall feed conversion ratio (1.00 ± 0.03 vs. 1.08 ± 0.03) and higher protein retention efficiency (30.5 ± 0.6 vs. 27.6 ± 0.6 %) than continuous feeding near-satiation. This resulted in reduced nitrogen discharge per tonne of fish produced in cages to the aquatic environment (45,5 ± 1.2 vs. 51.1 ± 0.8 kg t⁻¹). These results suggest that compensatory growth response not only enhances feed conversion efficiency but also leads to higher nitrogen retention efficiency and a reduced nitrogen discharge into the environment.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"327 ","pages":"Article 116402"},"PeriodicalIF":2.5,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth performance, meat quality, rumen morphometrics and microbiota of finishing bulls fed diets supplemented with oregano essential oils","authors":"Stella Dokou ,&nbsp;Ilias Giannenas ,&nbsp;Ioanna Stylianaki ,&nbsp;Eleftherios Bonos ,&nbsp;Vangelis Economou ,&nbsp;Georgios Arsenos","doi":"10.1016/j.anifeedsci.2025.116405","DOIUrl":"10.1016/j.anifeedsci.2025.116405","url":null,"abstract":"<div><div>The aim was to investigate the effects of microencapsulated oregano essential oil (OEO) dietary supplementation on growth performance, meat quality, rumen morphometrics and microbiota, in finishing bulls. Sixty-eight Limousin bulls were randomly allocated into 2 groups (CON: offered basal diet and OREG: basal diet supplemented with 50 mg OEO in microencapsulated form/kg dry matter). The trial lasted 90 days. Thereafter all bulls were slaughtered. At slaughter samples of <em>Longissimus dorsi</em> muscle (13^th rib steak) were collected to evaluate meat pH, color, fatty acids profile and oxidation status. Rumen tissue samples were collected to assess histomorphology. Additionally, rumen fluid samples were collected to examine bacterial diversity, through 16S rRNA sequencing. One of the key findings was that DMI was influenced by OEO dietary inclusion, with OEO supplemented bulls consuming less feed (P &lt; 0.01), without negatively affecting average daily gain and final body weight. In terms of meat quality OEO supplementation improved antioxidant status and caused a 47.31 % reduction in Elaidic acid (P &lt; 0.05). Rumen papillae length and width remained similar between groups (P &gt; 0.05), while a 32 % reduction in keratinized layer thickness (P = 0.034) was observed in OEO supplemented bulls. However, no differences were observed in meat pH and color (P &gt; 0.05). The analysis of rumen fluid samples revealed that beta-diversity was significantly altered (P = 0.009) and the abundance of <em>Succinivibrionaceae</em> family was higher (P &lt; 0.05) in OEO supplemented bulls, while <em>Rikenellaceae</em> RC9 gut group population decreased (P &lt; 0.05). Finally, glutathione peroxidase metabolic pathway, which is involved in protecting cells from oxidative damage, was upregulated by OEO supplementation. These findings suggest that microencapsulated OEO supplementation favorably modified rumen microbiome, contributing to reduced feed intake without compromising performance and meat quality in finishing bulls. Additionally, OEO supplementation in this study was associated with improvement in meat preservation.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"327 ","pages":"Article 116405"},"PeriodicalIF":2.5,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virtual screening and in vitro validation of methane inhibitor – Fraxin","authors":"Xiao Li ,&nbsp;Liu ZiHao ,&nbsp;Zhou MengTing ,&nbsp;Guo Jian ,&nbsp;Nan XueMei ,&nbsp;Tang XiangFang ,&nbsp;Xiong BenHai","doi":"10.1016/j.anifeedsci.2025.116404","DOIUrl":"10.1016/j.anifeedsci.2025.116404","url":null,"abstract":"<div><div>The production of methane (CH₄) poses a major challenge to both livestock and the environment, in particular contributing to the greenhouse effect, so reducing CH₄ emissions is urgent. In this study, methyl-coenzyme M reductase (MCR) was used as a target to screen natural product libraries, identify potential natural CH₄ inhibitors, and then verify their inhibitory effects on MCR and decreased in vitro CH₄. The results showed that fraxin and MCR scored well. In the follow-up verification, fraxin showed significant inhibitory activity on MCR, significantly decreased in vitro CH₄ production (<em>P</em> = 0.010), and significantly increased DMD (<em>P</em> = 0.049). The inhibition effect of 1 mg/gDM was the best, and the inhibition rate was 10.85 %. In this study, we found that fraxin can be used as a new feed additive that can inhibit CH₄.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"327 ","pages":"Article 116404"},"PeriodicalIF":2.5,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144212079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technical Note: Different analytical approaches in the quantification of microbial crude protein pool in abomasal digesta of nursling goat kids","authors":"Jardeson de Souza Pinheiro ,&nbsp;Paulo Sergio Dornelas Silva ,&nbsp;Isadora Emily Ribeiro ,&nbsp;Dhones Rodrigues Andrade ,&nbsp;Natalia Veloso Trópia ,&nbsp;Yame Fabres Robaina Sancler-Silva ,&nbsp;Erollykens Ferreira Santos ,&nbsp;Giarlã Cunha da Silva ,&nbsp;Simone Eliza Facione Guimarães ,&nbsp;Marcos Inacio Marcondes","doi":"10.1016/j.anifeedsci.2025.116399","DOIUrl":"10.1016/j.anifeedsci.2025.116399","url":null,"abstract":"<div><div>We evaluated methodologies to quantify microbial crude protein (MCP) pool in abomasum samples of pre-weaned dairy goat kids fed with milk replacer (MR). Sixty newborn male goat kids of the Saanen and Swiss Alpine breeds at an average age of 2 ± 1 days, and an average body weight (BW) of 3.834 ± 0.612 kg were included in the study. The goat kids received different liquid diet levels categorized as low nutritional plan (<strong>LNP</strong>; providing 1 liter of MR/goat kid/day), and a high nutritional plan (<strong>HNP</strong>; at 2 liters of MR/goat kid/day). All goat kids were harvested with 45 days of life at six-time points after feeding. Abomasum contents were sampled to determine the microbial pool and to estimate the MCP. Four methods were adopted for MCP quantification: yeast RNA – method 1, Bradford Blue – method 2 (<strong>BBM</strong>), Flow cytometry – method 3 (<strong>FCM</strong>; method proposed in the current study), and Polymerase Chain Reaction -method 4 (<strong>PCRM</strong>; method proposed in the current study). All four methods were compared with each other in 2 × 2 pairs. The yeast RNA method had the greatest estimate (1.03 g of MCP) compared to the other methods. Comparative analyses of the different methods presented the lowest variability data for PCRM with SD = 0.07. In conclusion, the four evaluated methods provided different MCP estimates. However, for precise MCP determination, we recommend the PCRM method, with FCM as a reliable alternative. These methods showed lower SD due to better control and primer specificity.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"327 ","pages":"Article 116399"},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of multi-species probiotic as a functional feed additive on immune response, health status, and offspring quality of female rainbow trout (Oncorhynchus mykiss) breeders","authors":"Erfan Akbari Nargesi ,&nbsp;Mir Masoud Sajjadi ,&nbsp;Bahram Falahatkar","doi":"10.1016/j.anifeedsci.2025.116403","DOIUrl":"10.1016/j.anifeedsci.2025.116403","url":null,"abstract":"<div><div>As a functional feed additive, probiotics have the potential to improve the health status of fish and enhance the quality of offspring. The present study aimed to investigate the effects of multi-species probiotic supplementation on immunological parameters, enzyme activities, stress response, and alevins' malformations in female rainbow trout (<em>Oncorhynchus mykiss</em>) breeders. To achieve this goal, a total of 60 three-year-old female breeders with an average initial weight of 2267.4 ± 54.9 g were randomly divided into four groups (in triplicate) and fed diets (twice a day up to satiation) containing 0 (P0), 1 × 10⁹ (P1), 2 × 10⁹ (P2), and 4 × 10⁹ (P4) colony-forming unit (CFU) of commercial multi-species probiotics (contains eight bacterial and one yeast species) kg⁻¹ of diet for eight weeks. In the experiment, fish were placed in twelve different sections (three sections per feed) in a flow-through system, each with a water volume of 4 m³ . Blood samples were collected after the end of the feeding trial from the caudal vein of three fish per replicate using a heparinized syringe both before and after stripping. According to the results, the complement component 3, complement component 4, and immunoglobulin M concentrations significantly increased in the P4 treatment compared to the P0 group (<em>P</em> &lt; 0.05). Plasma enzyme activities were significantly reduced following multi-strain probiotics administration in P2 and P1-treated fish (<em>P</em> &lt; 0.05). No significant differences were observed in plasma ions concentration among the experimental groups. For the stress indicators, breeders of P4 treatment showed significantly lower cortisol (41.0 ± 5.2 ng mL⁻¹) and glucose (151.5 ± 3.3 mg dL⁻¹) concentrations compared to the P0 fish, which had represented cortisol and glucose levels of 71.8 ± 4.0 ng mL⁻¹ and 184.9 ± 5.8 mg dL⁻¹, respectively (<em>P</em> &lt; 0.05). Moreover, the administration of multi-species probiotics decreased the percentage of yolk-sac resorption defects (up to 17.9 %) and total malformations (up to 0.24 %) in the offspring of P2 and P4 treatments (<em>P</em> &lt; 0.05). In conclusion, multi-species probiotics supplementation in the diet (i.e., the level of 4 × 10⁹ CFU kg⁻¹) of female rainbow trout breeders could be suggested as a functional feed additive to improve immune response, well-being, and offspring quality.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"327 ","pages":"Article 116403"},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feeding a bromoform-based feed additive for methane mitigation in beef cattle","authors":"T. Gyeltshen ,&nbsp;P.S. Alvarez-Hess ,&nbsp;S. Jacques ,&nbsp;M.J. Auldist ,&nbsp;F.C. Cowley","doi":"10.1016/j.anifeedsci.2025.116401","DOIUrl":"10.1016/j.anifeedsci.2025.116401","url":null,"abstract":"<div><div>Bromoform (CHBr₃) is a compound with strong methane-mitigating potential that binds to reduced vitamin B₁₂, thereby inhibiting the cobalamin-dependent methyltransferase step of methane formation. However, bromoform is highly volatile and requires stabilization. This experiment evaluated the methane (CH₄) mitigating effect of feeding Rumin8 Investigational Veterinary Product (Rumin8 IVP), a feed additive containing stabilized bromoform in an oil medium to beef cattle in a cross-over design experiment with two treatments and 10 animals, i.e., Control and Rumin8 IVP (with the active ingredient at 30 mg bromoform/kg dry matter). Methane and hydrogen (H₂) gases emitted were measured using respiration chambers. Rumen fluid and blood samples were collected 3 h post-feeding on the days steers exited the respiration chambers to assess rumen function and plasma biochemical parameters. On average, supplementing with Rumin8 IVP reduced CH₄ emission by 93 % and CH₄ yield by 94 %, and increased hydrogen (H₂) emission by more than 2.5-fold when compared to Control. There was a shift in the dissolved CH₄ and H₂ concentration in the rumen fluid samples consistent with the reduction in the CH₄ production and CH₄ yield. Supplementing Rumin8 IVP had no effect on feed dry matter intake or plasma biochemical parameters. Rumin8 IVP containing stabilized bromoform when fed at 30 mg bromoform/kg DMI is a promising feed additive to reduce enteric CH₄ emissions. However, further investigation is necessary to understand its long-term effect on the animal.</div></div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"326 ","pages":"Article 116401"},"PeriodicalIF":2.5,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144166563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}