甲烷抑制剂Fraxin的虚拟筛选及体外验证

IF 2.5 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2025-05-27 DOI:10.1016/j.anifeedsci.2025.116404

Xiao Li , Liu ZiHao , Zhou MengTing , Guo Jian , Nan XueMei , Tang XiangFang , Xiong BenHai

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引用次数: 0

摘要

甲烷（CH4）的产生对牲畜和环境都构成了重大挑战，特别是造成了温室效应，因此减少CH4的排放迫在眉睫。本研究以甲基辅酶M还原酶（methyl- coase M reductase， MCR）为靶点，筛选天然产物文库，鉴定潜在的天然CH4抑制剂，并验证其对MCR的抑制作用和体外降低CH4的作用。结果表明，fraxin和MCR得分较高。在后续验证中，fraxin对MCR有明显的抑制作用，显著降低体外CH4产量（P = 0.010），显著增加DMD （P = 0.049）。以1 mg/gDM的抑制效果最好，抑制率为10.85 %。在本研究中，我们发现fraxin可以作为一种抑制CH4的新型饲料添加剂。

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Virtual screening and in vitro validation of methane inhibitor – Fraxin

The production of methane (CH₄) poses a major challenge to both livestock and the environment, in particular contributing to the greenhouse effect, so reducing CH₄ emissions is urgent. In this study, methyl-coenzyme M reductase (MCR) was used as a target to screen natural product libraries, identify potential natural CH₄ inhibitors, and then verify their inhibitory effects on MCR and decreased in vitro CH₄. The results showed that fraxin and MCR scored well. In the follow-up verification, fraxin showed significant inhibitory activity on MCR, significantly decreased in vitro CH₄ production (P = 0.010), and significantly increased DMD (P = 0.049). The inhibition effect of 1 mg/gDM was the best, and the inhibition rate was 10.85 %. In this study, we found that fraxin can be used as a new feed additive that can inhibit CH₄.

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.