Journal of ultrastructure and molecular structure research最新文献_第8页

Lithium-induced structural changes in the cortical distal nephron localized by computer-assisted three-dimensional reconstruction 计算机辅助三维重建定位的锂致皮质远端肾元结构改变

Journal of ultrastructure and molecular structure research Pub Date : 1988-09-01 DOI: 10.1016/0889-1605(88)90038-9

Jens Dørup , Peter D. Ottosen , Sten Christensen

{"title":"Lithium-induced structural changes in the cortical distal nephron localized by computer-assisted three-dimensional reconstruction","authors":"Jens Dørup , Peter D. Ottosen , Sten Christensen","doi":"10.1016/0889-1605(88)90038-9","DOIUrl":"10.1016/0889-1605(88)90038-9","url":null,"abstract":"<div><p>Lithium treatment is known to cause tubule dilation in distal nephron segments both in rat and in man. However, due to the heterogeneous cell composition of the distal nephron and the cellular changes following lithium treatment, it has been difficult to identify the structurally changed segments. In this study we have therefore applied computer-assisted reconstruction of cortical distal nephron segments. Tubule dilation was demonstrated in connecting and initial collecting tubules and in the first part of cortical collecting ducts (CCD) whereas it was absent from distal straight and distal convoluted tubules. Principal cells (P cells) in the CCD showed swelling of the cytoplasm, accumulation of actin-like microfilaments, and abnormal arrangements of basolateral membranes. Connecting tubule cells (CNT cells) showed similar but less pronounced changes. Intercalated cells (I cells) showed an accumulation of vesicles in the apical cytoplasm and a reduced luminal surface area. Lesions in P and CNT cells may, at least in part, explain the diabetes insipidus and sodium loss found during lithium treatment. Proton secretion in I cells is probably mediated by an ATPase present in the luminal membrane. The reduction in area of this membrane may explain why lithium-treated animals have a lowered ability to excrete an acid load.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"100 3","pages":"Pages 212-223"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90038-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14372008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Further evidence for the correlation between the primary structure and the stain exclusion banding pattern of the segment-long-spacing crystallites of collagen 进一步证明胶原蛋白片段长间距晶体的初级结构与染色排除带模式之间的相关性

Journal of ultrastructure and molecular structure research Pub Date : 1988-09-01 DOI: 10.1016/0889-1605(88)90042-0

Kunihiko Kobayashi , Yoko Hashimoto , Taro Hayakawa , Takeshi Hoshino

{"title":"Further evidence for the correlation between the primary structure and the stain exclusion banding pattern of the segment-long-spacing crystallites of collagen","authors":"Kunihiko Kobayashi , Yoko Hashimoto , Taro Hayakawa , Takeshi Hoshino","doi":"10.1016/0889-1605(88)90042-0","DOIUrl":"10.1016/0889-1605(88)90042-0","url":null,"abstract":"<div><p>Recently, we have noted the direct correlation between the primary structure of type I collagen and the electron microscopical banding pattern of the negatively stained segment-long-spacing (SLS) crystallites (<span>K. Kobayashi, T. Ito, and T. Hoshino (1986)</span>, <em>J. Electr. Microsc.</em> <strong>35</strong>, 272–275). In this paper, we examined the correlation in the other types of collagen. Unstained light bands (stain excluding bands) of the negatively stained SLSs of type II and type III collagens were located at the clusters of large hydrophobic amino acid residues along the respective molecules. Photographic averaging of the pattern improved the visual comparison of the correlation. We also noted a few occasions of discrepancy from the above-mentioned correlation. Preliminary computer simulation experiments revealed that, among amino acid parameters so far reported, only the hydrophobicity values of <span>G. D. Rose and S. Roy (1980)</span>, <em>Proc. Natl. Acad. Sci. USA</em> <strong>77</strong>, 4643–4647) explained the ability of amino acids for the negative staining (stain exclusion) of the collagen SLSs.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"100 3","pages":"Pages 255-262"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90042-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13616307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Functional significance of intermediate filament meshwork in annelid helical muscles 环节动物螺旋肌中间丝网的功能意义

Journal of ultrastructure and molecular structure research Pub Date : 1988-08-01 DOI: 10.1016/0889-1605(88)90025-0

Magda de Eguileor, Franco Cotelli, Roberto Valvassori, Maurizio Brivio, Laura di Lernia

引用次数: 14

Cryo electron microscopy of unstained, unfixed RecA-cssDNA complexes 未染色、未固定RecA-cssDNA复合物的低温电子显微镜

Journal of ultrastructure and molecular structure research Pub Date : 1988-08-01 DOI: 10.1016/0889-1605(88)90023-7

Chung-Fu Chang , Debby A. Rankert , Tzyy-Wen Jeng , David G. Morgan, Michael F. Schmid , Wah Chiu

引用次数: 20

Concanavalin A binding sites on the surface of Drosophila melanogaster sperm: A fluorescence and ultrastructural study 果蝇精子表面刀豆蛋白A结合位点的荧光和超微结构研究

Journal of ultrastructure and molecular structure research Pub Date : 1988-08-01 DOI: 10.1016/0889-1605(88)90024-9

Maria Elisa Perotti, Antonella Riva

{"title":"Concanavalin A binding sites on the surface of Drosophila melanogaster sperm: A fluorescence and ultrastructural study","authors":"Maria Elisa Perotti, Antonella Riva","doi":"10.1016/0889-1605(88)90024-9","DOIUrl":"10.1016/0889-1605(88)90024-9","url":null,"abstract":"<div><p>The distribution of α-<span>d</span>-mannose/α-<span>d</span>-glucose terminal residues in the plasma membrane of <em>Drosophila melanogaster</em> spermatozoon has been investigated by fluorescence and electron microscopy using concanavalin A (Con A) labeling. The results indicate the presence of distinct domains on the sperm surface. Intense binding of Con A to the plasma membrane is highly restricted to the acrosomal region and to the endpiece of the tail. In the former, Con A receptors are not homogeneously distributed, suggesting the presence of microdomains in the acrosomal area. The main part of the tail contains very few Con A binding sites, which are confined to specific areas of the membrane. The sperm surface overlying the nucleus is completely negative. The labeling pattern is unchanged after storage in the female before fertilization. A preliminary analysis of the surface of mature oocytes using fluorochrome-conjugated horseradish peroxidase indicates that <span>d</span>-mannose binding molecules are specifically associated with the chorion of the micropyle anterior part, which might therefore be the site of a preliminary interaction between egg and spermatozoon.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"100 2","pages":"Pages 173-182"},"PeriodicalIF":0.0,"publicationDate":"1988-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90024-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14277162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

The projection structure of α-toxin from Staphylococcus aureus in human platelet membranes as analyzed by electron microscopy and image processing 用电子显微镜和图像处理分析金黄色葡萄球菌α毒素在人血小板膜中的投影结构

Journal of ultrastructure and molecular structure research Pub Date : 1988-08-01 DOI: 10.1016/0889-1605(88)90026-2

A. Olofsson, U. Kavéus, M. Thelestam , H. Hebert

引用次数: 20

An electron microscopic study of cauliflower mosaic virus-induced viroplasms: Unusual structures within the viroplasm matrix with possible functional significance in the viral replication cycle 花椰菜花叶病毒诱导的病毒质的电镜研究:病毒质基质内的异常结构在病毒复制周期中可能具有功能意义

Journal of ultrastructure and molecular structure research Pub Date : 1988-08-01 DOI: 10.1016/0889-1605(88)90019-5

D. Rodríguez, D. López-Abella, J.R. Díaz-Ruiz

引用次数: 6

Comparative ultrastructural localization of collagen types III, IV, VI and laminin in rat uterus and kidney 大鼠子宫和肾脏III型、IV型、VI型胶原和层粘连蛋白的超微结构定位比较

Journal of ultrastructure and molecular structure research Pub Date : 1988-08-01 DOI: 10.1016/0889-1605(88)90021-3

George Karkavelas , Nicholas A. Kefalides , Peter S. Amenta , Antonio Martinez-Hernandez

引用次数: 48

Ultrastructure and function of thrombocytes in a family with congenital bleeding tendency 先天性出血倾向家族血栓细胞的超微结构和功能

Journal of ultrastructure and molecular structure research Pub Date : 1988-08-01 DOI: 10.1016/0889-1605(88)90020-1

Z. Xu , B.A. Afzelius , L. Stigendal , A.-C. Teger-Nilsson

{"title":"Ultrastructure and function of thrombocytes in a family with congenital bleeding tendency","authors":"Z. Xu , B.A. Afzelius , L. Stigendal , A.-C. Teger-Nilsson","doi":"10.1016/0889-1605(88)90020-1","DOIUrl":"10.1016/0889-1605(88)90020-1","url":null,"abstract":"<div><p>Freshly isolated blood from controls and from three members of a family with congenital bleeding tendency were examined with respect to ultrastructure of platelets. The fixation protocol, which included tannic acid, permitted the visualization of substructures in the marginal bundle. Whereas nearly all platelets from the controls had a discoid shape, those from one of the patients (mother of the other two cases) were mostly flat or else roundish with two or three marginal bundles in different orientations. One portion of the thrombocyte fraction of each person was briefly exposed to adenosine diphosphate in order to activate the platelets and thereby to induce shape changes. Most platelets from the controls reacted as expected by rounding up and by projecting some pseudopods. A certain percentage (between 10 and 20%) of the microtubules in these activated platelets had 14 or even 15 protofilaments. The platelets from patients (and in particular the mother) either reacted by an abnormal bulging of the cytoplasm or did not react visibly at all. The microtubules retained their 13-subunit composition or in the abnormally reacted ones often had an open C-shaped cross-sectional profile. The bleeding tendency in this family might be due to a defect in the cytoskeleton of the platelet making it unable to react properly to stimuli.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"100 2","pages":"Pages 126-136"},"PeriodicalIF":0.0,"publicationDate":"1988-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90020-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14353740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nodulous corpuscles and mitochondrial inclusions in sertoli cells of deer hybrids 鹿杂交种支持细胞的结节小体和线粒体内含物

Journal of ultrastructure and molecular structure research Pub Date : 1988-08-01 DOI: 10.1016/0889-1605(88)90018-3

Francis Hrudka

{"title":"Nodulous corpuscles and mitochondrial inclusions in sertoli cells of deer hybrids","authors":"Francis Hrudka","doi":"10.1016/0889-1605(88)90018-3","DOIUrl":"10.1016/0889-1605(88)90018-3","url":null,"abstract":"<div><p>Unusual transformations of endoplasmic reticulum and mitochondria were identified in the Sertoli cells of deer hybrids. A portion of the mitochondrial population was converted from the common, flexible mitochondrial form into stiff rods, with an increased volume of the matrix and relative paucity of cristae. The matrix displayed arrays of tubuloid elements or precursors at various stages of assembly. The second and more conspicuous adaptation concerned the agranular endoplasmic reticulum, which frequently transformed into oval or elongate, often interconnected bodies. These bodies consisted of a dozen or more concentrically arranged fenestrated cisternae and a variable amount of glycogen in between. An integral and distinctive feature of the cisternous bodies was nodes, together constituting the so-called nodulous corpuscles (NC). The nodes appeared as a dense meshwork or lattice, which has the potential to generate a new set of cisternae and NC that interconnect into a chain or tridimensional complex. The course of NC evolution from precursors to mature complexes is proposed. Both organelle adaptations occurred independently and in only Sertoli cells. They are believed to be a product of hybridization in deer.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"100 2","pages":"Pages 107-117"},"PeriodicalIF":0.0,"publicationDate":"1988-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90018-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14353084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1