Journal of ultrastructure and molecular structure research最新文献_第7页

Nuclear compactness as assessed by ferrtin permeation: A critical evaluation of the method 通过铁素渗透评估核致密性：对该方法的关键评估

Journal of ultrastructure and molecular structure research Pub Date : 1988-10-01 DOI: 10.1016/0889-1605(88)90077-8

M. Carmo-Fonseca

引用次数: 2

Polymerization of turtle α-macroglobulin through newly exposed sulfhydryls reveals the location of ex-thiolester bonds 甲鱼α-巨球蛋白通过新暴露的巯基聚合揭示了前硫酯酶键的位置

Journal of ultrastructure and molecular structure research Pub Date : 1988-10-01 DOI: 10.1016/0889-1605(88)90082-1

Toshiya Osada, Masaaki Nishigai, Atsushi Ikai

{"title":"Polymerization of turtle α-macroglobulin through newly exposed sulfhydryls reveals the location of ex-thiolester bonds","authors":"Toshiya Osada, Masaaki Nishigai, Atsushi Ikai","doi":"10.1016/0889-1605(88)90082-1","DOIUrl":"https://doi.org/10.1016/0889-1605(88)90082-1","url":null,"abstract":"<div><p>Green turtle α-macroglobulin, which has previously been shown to contain thiolester bonds, formed linear polymers after being treated with proteinases. Biochemical analyses showed that the polymerization proceeded through disulfide-bond formation between monomers. The only sulfhydryl groups available for such polymerization after proteinase treatment were these created as the product of thiolester hydrolysis. Electron micrographs of polymers revealed H-shaped monomeric units aligned lengthwise in linear polymers. The average length per monomeric unit in the polymer estimated from the discrete distribution of polymer lengths was approximately 80% of the average length of free monomers, indicating that monomers overlapped each other within a region of about 4 nm. From such observations we concluded that the newly produced sulfhydryl groups were located on the four arms of the H-shaped molecule. The location of sulfhydryls can be taken as the site of the exposure of thiolesters which were originally sequestered in the hydrophobic interior of the molecule. Since the structure of turtle α-macroglobulin is very similar to that of human serum <em>α</em><sub>2</sub>-macroglobulin the results predict a similar location of sulfhydryls in human <em>α</em><sub>2</sub>-macroglobulin after proteinase treatment. The observed polymerization property is unique to sea turtle α-macroglobulin and has not been observed with human <em>α</em><sub>2</sub>-macroglobulin or other homologous proteins.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"101 1","pages":"Pages 62-74"},"PeriodicalIF":0.0,"publicationDate":"1988-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90082-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72282762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Polarity and length of actin filaments at the fascia adherens of the cardiac intercalated disk 肌动蛋白丝的极性和长度在心脏间插盘的筋膜粘附

Journal of ultrastructure and molecular structure research Pub Date : 1988-09-01 DOI: 10.1016/0889-1605(88)90040-7

M. Yamaguchi, S. Yamano, M. Muguruma, R.M. Robson

引用次数: 18

Author index for volume 100 第100卷的作者索引

Journal of ultrastructure and molecular structure research Pub Date : 1988-09-01 DOI: 10.1016/0889-1605(88)90046-8

引用次数: 0

Plasmalemma deposits in tissues infected with lettuce infectious yellows virus 莴苣感染黄病毒后组织质膜沉积

Journal of ultrastructure and molecular structure research Pub Date : 1988-09-01 DOI: 10.1016/0889-1605(88)90041-9

Robin L. Pinto, Lynn L. Hoefert, Gail L. Fail

引用次数: 15

Intracellular crystal formation in Bacteroides nodosus 结节拟杆菌的细胞内晶体形成

Journal of ultrastructure and molecular structure research Pub Date : 1988-09-01 DOI: 10.1016/0889-1605(88)90043-2

Paul M. Hine

引用次数: 5

Sequential appearance of capsid protein and cylindrical inclusion protein in root-tip cells following infection with passion fruit woodiness virus 百香果木纹病毒感染后根尖细胞衣壳蛋白和圆柱形包涵蛋白的顺序出现

Journal of ultrastructure and molecular structure research Pub Date : 1988-09-01 DOI: 10.1016/0889-1605(88)90037-7

Na-Sheng Lin , Nancy Wang , Yau-Heiu Hsu

{"title":"Sequential appearance of capsid protein and cylindrical inclusion protein in root-tip cells following infection with passion fruit woodiness virus","authors":"Na-Sheng Lin , Nancy Wang , Yau-Heiu Hsu","doi":"10.1016/0889-1605(88)90037-7","DOIUrl":"10.1016/0889-1605(88)90037-7","url":null,"abstract":"<div><p>Early events in passion fruit woodiness virus (PWV) infection of root-tip cells of tobacco (<em>Nicotiana benthamiana</em>) were examined by immunoelectron microscopy with antisera prepared specifically against the capsid protein (CP) and cylindrical inclusion protein (CIP) of the virus. Capsid protein was first detected on the cytoplasmic virus-specific vesicles coincident with first appearance of virions, but virions were not visible at every location that was stained. Early configurations of cylindrical inclusions (CI) were first observed on the surface of endoplasmic reticulum. At a later stage when aggregates of PWV were present, PWV virions were intimately associated with CIs either within the cytoplasm or attached perpendicularly to the cell wall. Most PWV aggregates were bound to cytoplasmic membranous structures in cells at a late stage of infection. Antiserum against CP partially reacted with CIs at advanced stages of infection. On the other hand, antiserum against CIP reacted only with CIs, but not with PWV virions. Both CP and CIP could also be detected in the plasmodesmata of infected cells.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"100 3","pages":"Pages 201-211"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90037-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53916799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Abstract from the fortieth anniversary meeting of the scandinavian society for electron microscopy 斯堪的纳维亚电子显微镜学会四十周年会议摘要

Journal of ultrastructure and molecular structure research Pub Date : 1988-09-01 DOI: 10.1016/0889-1605(88)90045-6

引用次数: 0

Inhibitory effect of phenobarbital on peroxisome biogenesis in mouse hepatocytes 苯巴比妥对小鼠肝细胞过氧化物酶体生物生成的抑制作用

Journal of ultrastructure and molecular structure research Pub Date : 1988-09-01 DOI: 10.1016/0889-1605(88)90044-4

Shinsuke Kanamura, Kazuo Kanai, Youko Asaka, Jun Watanabe

{"title":"Inhibitory effect of phenobarbital on peroxisome biogenesis in mouse hepatocytes","authors":"Shinsuke Kanamura, Kazuo Kanai, Youko Asaka, Jun Watanabe","doi":"10.1016/0889-1605(88)90044-4","DOIUrl":"10.1016/0889-1605(88)90044-4","url":null,"abstract":"<div><p>Peroxisomes in hepatocytes from mice administered 35, 50, or 100 mg/kg of phenobarbital (PB) were analyzed by quantitative electron microscopy. In perivenular hepatocytes, the volume of peroxisomes per unit cytoplasmic volume decreased to 78 or 57% of that of control animals by administration of 50 or 100 mg/kg. Their average volume also decreased to 78 or 64% of that in control animals in animals injected with 35 or 50 mg/kg. Further, cytochemical catalase activity appeared decreased in peroxisomes of hepatocytes of this zone after administration of 50 or 100 mg/kg. These suggest an inhibitory action of PB administration of biogenesis of peroxisomes in perivenular hepatocytes. In periportal hepatocytes, however, the volume density, average volume, and cytochemical catalase activity of peroxisomes did not change by administration of any doses of PB. Thus, PB administration appears to produce no inhibitory effect on the peroxisome biogenesis in periportal hepatocytes. On the other hand, the number of peroxisomes per unit cyto-plasmic volume increased in both periportal and perivenular hepatocytes in animals injected with 50 mg/kg, although it returned to the level of control animals by injection of 100 mg/kg. The inhibitory effect of PB on the peroxisome neogenesis in perivenular hepatocytes may be related to the marked smooth endoplasmic reticulum proliferation by administration of this drug.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"100 3","pages":"Pages 269-277"},"PeriodicalIF":0.0,"publicationDate":"1988-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90044-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14372009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Vitreous humor of chicken contains two fibrillar systems: An analysis of their structure 鸡玻璃体含有两个纤维系统:结构分析

Journal of ultrastructure and molecular structure research Pub Date : 1988-09-01 DOI: 10.1016/0889-1605(88)90039-0

David W. Wright, Richard Mayne

引用次数: 107