发布求助
文献互助 智能选刊 最新文献

Journal of ultrastructure and molecular structure research最新文献

筛选
英文 中文
Early changes in the substructure of the marginal bundle in human blood platelets responding to adenosine diphosphate 人血小板边缘束亚结构对二磷酸腺苷反应的早期改变
Journal of ultrastructure and molecular structure research Pub Date : 1988-06-01 DOI: 10.1016/0889-1605(88)90069-9
Z. Xu , B.A. Afzelius
{"title":"Early changes in the substructure of the marginal bundle in human blood platelets responding to adenosine diphosphate","authors":"Z. Xu ,&nbsp;B.A. Afzelius","doi":"10.1016/0889-1605(88)90069-9","DOIUrl":"10.1016/0889-1605(88)90069-9","url":null,"abstract":"<div><p>Human blood platelets were exposed to 10 μ<em>M</em> adenosine diphosphate in order to induce shape changes and their ultrastructure was examined in preparations fixed with a glutaraldehyde-tannic acid mixture. Within 5 sec after the addition of ADP the marginal bundle along the perimeter of the platelet had undergone profound changes. The intertubular filaments of the marginal bundle were lost and, probably as a consequence, the microtubules were dispersed. The microtubules also had fragmented and appeared as straight rods rather than smoothly curved coils. Profiles with 14 or 15 protofilaments rather than the common 13-unit pattern were seen in 16% of the cross-sectioned microtubules. The proportion of incomplete (C-shaped) microtubules or of microtubules with an electron-dense interior also had increased and were interpreted as sections near the terminal ends of a microtubule. We interpret the C-shaped and the “filled” microtubules as signs of a disassembled microtubule and the 14- and 15-protofilament profiles as probably representing new (ends of) microtubules reassembled with a defective control of the proper structure.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Pages 254-260"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90069-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14328390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Immunocytochemical analysis of trichocyst structure and development inParamecium 草履虫毛囊结构与发育的免疫细胞化学分析
Journal of ultrastructure and molecular structure research Pub Date : 1988-06-01 DOI: 10.1016/0889-1605(88)90065-1
Klaus Hausmann , Agnes K. Fok , Richard D. Allen
{"title":"Immunocytochemical analysis of trichocyst structure and development inParamecium","authors":"Klaus Hausmann ,&nbsp;Agnes K. Fok ,&nbsp;Richard D. Allen","doi":"10.1016/0889-1605(88)90065-1","DOIUrl":"10.1016/0889-1605(88)90065-1","url":null,"abstract":"<div><p>The structure and development of<em>Paramecium multimicronucleatum</em> trichocysts have been analyzed using a trichocyst core-specific monoclonal antibody (MAb) and indirect immunogold labeling on LR-Gold-embedded as well as on fixed, frozen, and cryosectioned material. The resting trichocyst body consistently shows a stain-free 150-nm-wide cortical region and a positively reacting core. Neither the cap nor the paracrystalline core of the tip is labeled. During trichocyst morphogenesis small immunogold-positive vesicles fuse to form larger vacuoles. A paracrystalline core forms within the vacuole matrix. The label becomes progressively concentrated within this core and, ultimately, is restricted entirely to the core. The trichocyst mutants of<em>Paramecium tetraurelia, st A, tam 8, pt A<sup>1</sup></em>, and<em>ft A<sup>3</sup></em>, show an almost unaltered trichocyst body with the same staining properties as wild-type trichocysts. These mutations affect mainly the structure and location of the trichocyst tip. In the trichless<em>tl</em> mutant the contents of small cytoplasmic vesicles crossreact with the MAb.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Pages 213-225"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90065-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53917422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 25
Author index for volume 98 第98卷作者索引
Journal of ultrastructure and molecular structure research Pub Date : 1988-06-01 DOI: 10.1016/0889-1605(88)90072-9
{"title":"Author index for volume 98","authors":"","doi":"10.1016/0889-1605(88)90072-9","DOIUrl":"https://doi.org/10.1016/0889-1605(88)90072-9","url":null,"abstract":"","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Page 273"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90072-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72216736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The substructure of marginal bundles in human blood platelets 人血小板边缘束的亚结构
Journal of ultrastructure and molecular structure research Pub Date : 1988-06-01 DOI: 10.1016/0889-1605(88)90068-7
Z. Xu , B.A. Afzelius
{"title":"The substructure of marginal bundles in human blood platelets","authors":"Z. Xu ,&nbsp;B.A. Afzelius","doi":"10.1016/0889-1605(88)90068-7","DOIUrl":"10.1016/0889-1605(88)90068-7","url":null,"abstract":"<div><p>A fixation protocol was worked out which permitted the visualization of the substructure of microtubules in human blood platelets. This protocol included a brief prefixation with 0.1% glutaraldehyde in a “polymerization mix buffer” including GTP, followed by a fixation mixture containing glutaraldehyde and tannic acid, and postfixation in 0.1% osmium tetroxide. The 13 individual protofilaments of the microtubules were clearly visible and had a triangular cross-sectional area. Filaments could be seen running alongside the microtubules and connected to them by many links. We term these filaments the “intertubular filaments.” Some microtubules were incomplete and had a C-shaped profile; by serial sectioning it was seen that such microtubules were close to the terminal ends of the microtubules. A low percentage of the platelets had two marginal bundles oriented perpendicularly to each other.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Pages 244-253"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90068-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14273324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Intracellular localization of TEV capsid and inclusion proteins by immunogold labeling 免疫金标记技术在TEV衣壳和包涵蛋白细胞内的定位
Journal of ultrastructure and molecular structure research Pub Date : 1988-06-01 DOI: 10.1016/0889-1605(88)90064-X
David Baunoch, Pritam Das, V. Hari
{"title":"Intracellular localization of TEV capsid and inclusion proteins by immunogold labeling","authors":"David Baunoch,&nbsp;Pritam Das,&nbsp;V. Hari","doi":"10.1016/0889-1605(88)90064-X","DOIUrl":"10.1016/0889-1605(88)90064-X","url":null,"abstract":"<div><p>Thin sections of tobacco etch virus (TEV)-infected leaves were treated with goat anti-rabbit antibodies conjugated to 15-nm gold particles after the sections had been exposed to rabbit antisera specific to virus particles, virus capsid, 70K CI protein, 54K NI protein, or 49K NI protein. Anti-TEV or anti-TEV capsid sera specifically detected virus particles and capsid protein in the cytoplasm and capsid protein which was also found arrayed in a regular fashion adjacent to the cell wall. The NI 54K and 49K inclusions were found primarily in the nucleus and surprisingly in the cytoplasm. Free NI 54K and 49K proteins were also found in regions adjacent to the nuclear membrane and in association with the nucleolus. The 70K CI protein was found in the pinwheel inclusions and laminated aggregates as well as filamentous structures associated with the cell membrane. The latter observation is significant in view of the hypothesis that the 70K CI protein represents the membrane binding component of the viral replication complex.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Pages 203-212"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90064-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53917272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Morphological and functional reconsideration of the cytoplasmic bridges which connect male germ cells in snails 蜗牛雄性生殖细胞连接细胞质桥的形态学和功能重新研究
Journal of ultrastructure and molecular structure research Pub Date : 1988-06-01 DOI: 10.1016/0889-1605(88)90070-5
Fumioki Yasuzumi, Nobuhiko Okura, Yutaka Kohata, Kentaro Harutsugu
{"title":"Morphological and functional reconsideration of the cytoplasmic bridges which connect male germ cells in snails","authors":"Fumioki Yasuzumi,&nbsp;Nobuhiko Okura,&nbsp;Yutaka Kohata,&nbsp;Kentaro Harutsugu","doi":"10.1016/0889-1605(88)90070-5","DOIUrl":"10.1016/0889-1605(88)90070-5","url":null,"abstract":"<div><p>Cytoplasmic bridges (CB) between male germ cells of three fresh-water snails have been examined by electron microscopy, using ultrathin sections and freeze-fracture replicas prepared by ordinary methods and after use of filipin for indicating the presence of membrane cholesterol. The bridge plasma membrane, which was formerly considered to be smooth, was found in these snails to be corrugated. The corrugations were periodically parallel and oriented parallel to the axis of the bridge. The mature bridges showed very low densities of intramembranous particles. No filipin-sterol complexes formed on either the P face or the E face of the bridge plasma membrane, in contrast to plasma membranes elsewhere. The numbers of corrugations in each CB varied with the species. The membrane corrugations overlie bundles of electron dense fibers measuring approximately 30 nm in diameter and 60 nm in center-to-center distance, fitting into convexities of the plasma membrane. The present observations lead us to the necessity of reconsidering the morphological and functional aspects of cytoplasmic bridges in vertebrate as well as in invertebrate germ cells.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Pages 261-271"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90070-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14328279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Head structure of bacteriophages T2 and T4 噬菌体T2和T4的头部结构
Journal of ultrastructure and molecular structure research Pub Date : 1988-06-01 DOI: 10.1016/0889-1605(88)90063-8
Werner Baschong , Ueli Aebi , Cristina Baschong-Prescianotto , Jaques Dubochet , Lukas Landmann , Eduard Kellenberger , Michel Wurtz
{"title":"Head structure of bacteriophages T2 and T4","authors":"Werner Baschong ,&nbsp;Ueli Aebi ,&nbsp;Cristina Baschong-Prescianotto ,&nbsp;Jaques Dubochet ,&nbsp;Lukas Landmann ,&nbsp;Eduard Kellenberger ,&nbsp;Michel Wurtz","doi":"10.1016/0889-1605(88)90063-8","DOIUrl":"10.1016/0889-1605(88)90063-8","url":null,"abstract":"<div><p>The length-to-width ratios of bacteriophage T2 and T4 heads and stereometric angles specifying the prolate icosahedral T2 capsid were evaluated on electron micrographs recorded from samples prepared by a variety of methods. The copy numbers of the major capsid protein, gp23*, of T2 and T4 phages were compared by quantitative gel electrophoresis. Taken together, the resulting values are most compatible with triangulation numbers <em>T</em> = 13 and <em>Q</em> = 21 for both T2 and T4, thus confirming the previously proposed capsid architecture of T4 revealed by indirect measurements and thereby eliminating the repeatedly reported discrepancy between T2 and T4 in favor of a common <em>Q</em> number of 21 corresponding to 960 copies of gp23*.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Pages 189-202"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90063-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14328389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 32
Development and aging of leaf etioplasts in maize cultured with and without sucrose 加糖和不加糖玉米叶片腐殖体的发育和老化
Journal of ultrastructure and molecular structure research Pub Date : 1988-06-01 DOI: 10.1016/0889-1605(88)90066-3
Nicoletta Rascio, Paola Mariani, Francesca Dalla Vecchia, Pasquale Chitano
{"title":"Development and aging of leaf etioplasts in maize cultured with and without sucrose","authors":"Nicoletta Rascio,&nbsp;Paola Mariani,&nbsp;Francesca Dalla Vecchia,&nbsp;Pasquale Chitano","doi":"10.1016/0889-1605(88)90066-3","DOIUrl":"10.1016/0889-1605(88)90066-3","url":null,"abstract":"<div><p>The vital cycle of etioplasts has been compared in leaves of etiolated plants of maize grown on inorganic medium with or without sucrose, in order to ascertain how much starvation might affect the start of senescence. The results showed that the sucrose supply lengthened leaf survival and delayed the appearance of the late ultrastructural symptoms of etioplast senescence, but did not affect the beginning of senescence, which can be evaluated as a decrease in protochlrophyll(ide) contents. The start of senescence in etiolated leaves of maize seems to be a preprogrammed event rather than one dependent on the occurrence of tissue starvation.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Pages 226-233"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90066-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53917465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Contents of volume 98 第98卷目录
Journal of ultrastructure and molecular structure research Pub Date : 1988-06-01 DOI: 10.1016/0889-1605(88)90073-0
{"title":"Contents of volume 98","authors":"","doi":"10.1016/0889-1605(88)90073-0","DOIUrl":"https://doi.org/10.1016/0889-1605(88)90073-0","url":null,"abstract":"","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Pages 274-276"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90073-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72256684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assembly of two-dimensional membrane crystals of Na,K-ATPase Na, k - atp酶二维膜晶体的组装
Journal of ultrastructure and molecular structure research Pub Date : 1988-06-01 DOI: 10.1016/0889-1605(88)90067-5
Margareta So¨derholm , Hans Hebert , Elisabeth Skriver , Arvid B. Maunsbach
{"title":"Assembly of two-dimensional membrane crystals of Na,K-ATPase","authors":"Margareta So¨derholm ,&nbsp;Hans Hebert ,&nbsp;Elisabeth Skriver ,&nbsp;Arvid B. Maunsbach","doi":"10.1016/0889-1605(88)90067-5","DOIUrl":"10.1016/0889-1605(88)90067-5","url":null,"abstract":"<div><p>The assembly of vanadate-induced two-dimensional membrane crystals of Na,K-ATPase was analyzed by electron microscopy and image processing. Electron micrographs of negatively stained linear arrays of protein molecules were recorded and processed by correlation averaging methods. The arrays were compared with fully developed p21 crystals of the enzyme. On the basis of similarity in protein form, symmetry, and packing arrangement it was concluded that the fully developed crystals are built of tightly packed ribbons. Assembly pathways for two-dimensional membrane crystals of Na,K-ATPase are proposed.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Pages 234-243"},"PeriodicalIF":0.0,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90067-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13986900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
首页 上一页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信