Margareta So¨derholm , Hans Hebert , Elisabeth Skriver , Arvid B. Maunsbach
{"title":"Na, k - atp酶二维膜晶体的组装","authors":"Margareta So¨derholm , Hans Hebert , Elisabeth Skriver , Arvid B. Maunsbach","doi":"10.1016/0889-1605(88)90067-5","DOIUrl":null,"url":null,"abstract":"<div><p>The assembly of vanadate-induced two-dimensional membrane crystals of Na,K-ATPase was analyzed by electron microscopy and image processing. Electron micrographs of negatively stained linear arrays of protein molecules were recorded and processed by correlation averaging methods. The arrays were compared with fully developed p21 crystals of the enzyme. On the basis of similarity in protein form, symmetry, and packing arrangement it was concluded that the fully developed crystals are built of tightly packed ribbons. Assembly pathways for two-dimensional membrane crystals of Na,K-ATPase are proposed.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"99 3","pages":"Pages 234-243"},"PeriodicalIF":0.0000,"publicationDate":"1988-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(88)90067-5","citationCount":"9","resultStr":"{\"title\":\"Assembly of two-dimensional membrane crystals of Na,K-ATPase\",\"authors\":\"Margareta So¨derholm , Hans Hebert , Elisabeth Skriver , Arvid B. Maunsbach\",\"doi\":\"10.1016/0889-1605(88)90067-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The assembly of vanadate-induced two-dimensional membrane crystals of Na,K-ATPase was analyzed by electron microscopy and image processing. Electron micrographs of negatively stained linear arrays of protein molecules were recorded and processed by correlation averaging methods. The arrays were compared with fully developed p21 crystals of the enzyme. On the basis of similarity in protein form, symmetry, and packing arrangement it was concluded that the fully developed crystals are built of tightly packed ribbons. Assembly pathways for two-dimensional membrane crystals of Na,K-ATPase are proposed.</p></div>\",\"PeriodicalId\":77743,\"journal\":{\"name\":\"Journal of ultrastructure and molecular structure research\",\"volume\":\"99 3\",\"pages\":\"Pages 234-243\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0889-1605(88)90067-5\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of ultrastructure and molecular structure research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0889160588900675\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of ultrastructure and molecular structure research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0889160588900675","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9
摘要
利用电子显微镜和图像处理技术对钒酸盐诱导的Na, k - atp酶二维膜晶体的组装过程进行了分析。记录蛋白质分子负染色线性阵列的电子显微图，并用相关平均法处理。将这些阵列与酶的完全发育的p21晶体进行比较。基于蛋白质形态、对称性和排列排列的相似性，我们得出结论:完全发育的晶体是由紧密排列的条带构成的。提出了Na、k - atp酶二维膜晶体的组装途径。
Assembly of two-dimensional membrane crystals of Na,K-ATPase
The assembly of vanadate-induced two-dimensional membrane crystals of Na,K-ATPase was analyzed by electron microscopy and image processing. Electron micrographs of negatively stained linear arrays of protein molecules were recorded and processed by correlation averaging methods. The arrays were compared with fully developed p21 crystals of the enzyme. On the basis of similarity in protein form, symmetry, and packing arrangement it was concluded that the fully developed crystals are built of tightly packed ribbons. Assembly pathways for two-dimensional membrane crystals of Na,K-ATPase are proposed.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
