Journal of ultrastructure and molecular structure research最新文献

Two-dimensional crystalline arrays of Na,K-ATPase with new subunit interactions induced by cobalt-tetrammine-ATP 具有钴-四胺- atp诱导的新亚基相互作用的Na, k - atp酶的二维晶体阵列

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI: 10.1016/0889-1605(89)90013-X

Elisabeth Skriver , Arvid B. Maunsbach , Hans Hebert , Georgios Scheiner-bobis , Wilhelm Schoner

引用次数: 24

Insulin absorption in renal proximal tubules: A quantitative immunocytochemical study 肾近端小管胰岛素吸收:定量免疫细胞化学研究

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI: 10.1016/0889-1605(89)90015-3

Søren Nielsen , Erik Ilsø Christensen

{"title":"Insulin absorption in renal proximal tubules: A quantitative immunocytochemical study","authors":"Søren Nielsen , Erik Ilsø Christensen","doi":"10.1016/0889-1605(89)90015-3","DOIUrl":"10.1016/0889-1605(89)90015-3","url":null,"abstract":"<div><p>The purposes of the present study are mainly biological concerning proximal tubular handling of insulin: we will study the intracellular transport to subcellular compartments involved in insulin degradation, the specificity and saturability of the luminal endocytic absorption of insulin, the visualization of transtubular transport, and finally, if possible, the evaluation of the relative distribution (accumulation) of insulin in endocytic vacuoles and lysosomes. The second part is methodological: application of quantitative immunocytochemistry to endocytosis, quantitation of the effect of particle size and antigen density on labeling density on tissue sections, labeling at very low antigen densities, and effect of fish gelatin on background. Isolated renal proximal tubules were perfused with native insulin, <sup>125</sup>I-insulin, or [leucine<sup>B-25</sup>]-insulin (2% receptor-binding ability and full immunoreactivity) or exposed to native insulin from the basolateral membranes. In conclusion, the luminal uptake of insulin is of low specificity, as native and [leucine<sup>B-25</sup>]-insulin were accumulated to the same extent. Endocytic uptake is of high capacity and the mechanism is saturable. Insulin accumulated in endocytic vacuoles and lysosomes, thus following the classical degradation pathway. No other subcellular compartment is associated with insulin degradation. It was not possible to detect the basolateral uptake, indicating loss of immunoreactivity after binding to its receptor. Absolute quantitative immunocytochemistry is applicable in studying endocytosis. The labeling density increases nonproportionally with antigen density probably caused by steric hindrances. Reduction of the particle size (16 to 6 nm) increased the labeling density 17.6 times.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"102 3","pages":"Pages 205-220"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(89)90015-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13840263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Quantitation of corneal fibril diameters from bony fish using low temperature preparative methods 低温制备法定量硬骨鱼角膜原纤维直径

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI: 10.1016/0889-1605(89)90022-0

Alan S. Craig , Dav1d A.D. Parry

引用次数: 5

Intramitochondrial helical filaments in medullary tubules of the rat kidney 大鼠肾小管线粒体内螺旋状细丝

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI: 10.1016/0889-1605(89)90017-7

Hiroyuki Sasaki, Teruo Suzuki

引用次数: 4

Influence of exogenous sucrose on the greening of oat 外源蔗糖对燕麦绿化的影响

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI: 10.1016/0889-1605(89)90019-0

Gabriella Pasqua , Francesca Dalla Vecchia , Nicoletta Rascio , Giorgio Casadorot

引用次数: 3

Scanning transmission electron microscopic study of molluscan hemocyanins in various aggregation states: comparison with light scattering molecular weights 软体动物不同聚集状态下血青素的扫描透射电镜研究:与光散射分子量的比较

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI: 10.1016/0889-1605(89)90016-5

Mary G. Hamilton , Theodore T. Herskovits , Paul S. Furcinitti , Joseph S. Wall

{"title":"Scanning transmission electron microscopic study of molluscan hemocyanins in various aggregation states: comparison with light scattering molecular weights","authors":"Mary G. Hamilton , Theodore T. Herskovits , Paul S. Furcinitti , Joseph S. Wall","doi":"10.1016/0889-1605(89)90016-5","DOIUrl":"10.1016/0889-1605(89)90016-5","url":null,"abstract":"<div><p>The masses of individual particles of the hemocyanins of six members of two molluscan classes, Polyplacophora and Gastropods, have been determined by scanning transmission electron microscopy (STEM) of unstained specimens dried from the frozen state. The decameric hemocyanins of two chitons, <em>Mopalia muscosa</em> and <em>Stenoplax conapicua</em>, had masses of 4.20 ± 0.18 and 4.47 ± 0.56 MDa, respectively; the didecameric hemocyanins of two gastropods, <em>Fasciolaria tulipa</em> and <em>Pleuroploca gigantea</em>, had masses of 8.67 ± 0.44 and 8.96 ± 0.39 MDa, respectively; and the tridecameric hemocyanin of <em>Lunatia heros</em> had a mass of 13.50 ± 0.44 MDa. The STEM values were in close agreement with those obtained by light scattering measurements of the same samples in solution. For <em>Busycon centrarium</em>, a gastropod with a multidecameric hemocyanin, nine size classes from didecamers to decadecamers with masses that corresponded to multiples of a basic decamer (4.4 MDa) were detected. The appearance of unstained specimens of the cylindrical particles differs from negatively stained specimens. Viewed end-on the cylinders show no internal structure, but in well-preserved specimens cavities are apparent in the side views of the cylinders that resemble those seen in negatively stained specimens. Although they lack the characteristic “tiered” appearance, the number of decameric units can be counted and their arrangement within the particle seen.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"102 3","pages":"Pages 221-228"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(89)90016-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13777216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Quaternary structure of the immunostimulating complex (Iscom) 免疫刺激复合物(Iscom)的四元结构

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI: 10.1016/0889-1605(89)90018-9

Muhsin Özel , Stefan Hℷlund , Hans R. Gelderblom , Bror Morein

{"title":"Quaternary structure of the immunostimulating complex (Iscom)","authors":"Muhsin Özel , Stefan Hℷlund , Hans R. Gelderblom , Bror Morein","doi":"10.1016/0889-1605(89)90018-9","DOIUrl":"10.1016/0889-1605(89)90018-9","url":null,"abstract":"<div><p>Proteins of either HIV-1, hepatitis B, or rabies virus were incorporated with the adjuvant substance Quil A and cholesterol into the immunostimulating complex: iscom. Formation and symmetry of this regular complex were analyzed by electron microscopy. Micellar structures with a diameter of about 12 nm, occasionally with a 7-nm stain-filled center, were formed in a 0.03% water suspension of Quil A. Cavities or holes appeared in the smooth structures of cholesterol upon the addition ofing Quil A, and after mixing Quil A and cholesterol 1:1 fragile and flattened structures of matrix were produced withth a diameter of about 40 nm. By freeze-drying the matrix was preserved as a cage-like, isometric particle. Stable iscom particles composed of Quil A, cholesterol, and selected viral proteins had an approximate diameter of 32 nm. The particles had an uniform, cage-like structure, exhibiting icosahedral symmetry, irrespective of the viral proteins incorporated. Tilting experiments and rotational image analysis indicated that the iscoms were composed of 20 morphological subunits assembled in a pentagonal dodecahedron with a hole on each of the 12 pentagonal faces. The symmetrical shape of the iscom might explain both its remarkable stability and its capacity to efficiently present antigens to the immune system.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"102 3","pages":"Pages 240-248"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(89)90018-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13777217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 84

Cytopathology of satellite tobacco mosaic virus and its helper virus in tobacco 烟草卫星花叶病毒及其辅助病毒的细胞病理学

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI: 10.1016/0889-1605(89)90014-1

Kyung-Soo Kim , Rodrigo A. Valverde , J. Allan Dodds

{"title":"Cytopathology of satellite tobacco mosaic virus and its helper virus in tobacco","authors":"Kyung-Soo Kim , Rodrigo A. Valverde , J. Allan Dodds","doi":"10.1016/0889-1605(89)90014-1","DOIUrl":"10.1016/0889-1605(89)90014-1","url":null,"abstract":"<div><p>Ultrastructural responses of tobacco cells infected with a newly discovered satellite virus (STMV) that has an isometric morphology and is associated with rigid rodshaped tobacco mosaic virus (TMV) were studied <em>in situ</em>. In cells infected with TMV alone,TMV particles occurred as crystalline arrays in the cytoplasm and were usually associated with TMV-characteristic X bodies. In cells infected with both TMV and STMV, particles of STMV occurred only in cells that contained TMV particles, which suggests a correlation between the satellite and helper virus presence. However, the replication and/or accumulation sites of STMV appear to be independent from its helper virus. Unlike TMV particles, STMV particles were associated with several cytopathic structures such as granular inclusions, membranous vesicles of 50–80 nm, and myelin-like bodies which were all bounded by a single common membrane, No X bodies occurred in cells containing STMV particles, and the mitochondria possessed abnormal tubular structures containing flocculent material.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"102 3","pages":"Pages 196-204"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(89)90014-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53917960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Structural analysis of the S-Layer of Lampropedia hyalina 透明斑藻s层结构分析

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI: 10.1016/0889-1605(89)90020-7

John W. Austin , Andreas Engel , R.G.E. Murray , Ueli Aeb

{"title":"Structural analysis of the S-Layer of Lampropedia hyalina","authors":"John W. Austin , Andreas Engel , R.G.E. Murray , Ueli Aeb","doi":"10.1016/0889-1605(89)90020-7","DOIUrl":"10.1016/0889-1605(89)90020-7","url":null,"abstract":"<div><p>The two-dimensional (2D) structure of the regularly structured surface layer (S-layer) of the gram-negative eubacterium <em>Lampropedia hyalina</em> has been determined at the molecular level to a nominal resolution of 2.1 nm by transmission electron microscopy and digital image processing. The inner, or “perforate,” layer consists of dimeric block-shaped units located at two-fold symmetry axes. These morphological dimers associate around three-fold symmetry axes to form a continuous layer with p6 symmetry and a lattice constant of 14.6 ± 0.4 nm. Scanning transmission electron microscopy (STEM) yields a mass-per-area (MPA) value for the perforate layer of 3.5 kDa/nm<sup>2</sup>. The outer, or “punctate,” layer is composed of long, roughly cylindrical units centered on six-fold symmetry axes, which are connected by six fine linking arms joining at the three-fold symmetry axes to create a hexagonal layer with a lattice constant of 25.6 ± 0.5 nm. The MPA of the “composite”-i.e., perforate plus punctate—layer is 10.2 kDa/nm<sup>2</sup>.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"102 3","pages":"Pages 255-264"},"PeriodicalIF":0.0,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(89)90020-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"53918015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Cumulative subject index for volume 102 第102卷的累计受试者指数

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI: 10.1016/0889-1605(89)90025-6

引用次数: 0