Elisabeth Skriver , Arvid B. Maunsbach , Hans Hebert , Georgios Scheiner-bobis , Wilhelm Schoner
{"title":"具有钴-四胺- atp诱导的新亚基相互作用的Na, k - atp酶的二维晶体阵列","authors":"Elisabeth Skriver , Arvid B. Maunsbach , Hans Hebert , Georgios Scheiner-bobis , Wilhelm Schoner","doi":"10.1016/0889-1605(89)90013-X","DOIUrl":null,"url":null,"abstract":"<div><p>Purified membrane-bound Na,K-ATPase incubated with cobalt-tetrammine-ATP [Co(NH<sub>3</sub>)<sub>4</sub>ATP], which is a stable MgATP complex analog, shows two new types of membrane crystals, a new p21 form and a p4 form. The building blocks of the crystalline arrays correspond to (<em>αβ</em>)<sub>2</sub> dimers of the enzyme protein suggesting that α-α interaction may be important in the pumping process.</p></div>","PeriodicalId":77743,"journal":{"name":"Journal of ultrastructure and molecular structure research","volume":"102 3","pages":"Pages 189-195"},"PeriodicalIF":0.0000,"publicationDate":"1989-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(89)90013-X","citationCount":"24","resultStr":"{\"title\":\"Two-dimensional crystalline arrays of Na,K-ATPase with new subunit interactions induced by cobalt-tetrammine-ATP\",\"authors\":\"Elisabeth Skriver , Arvid B. Maunsbach , Hans Hebert , Georgios Scheiner-bobis , Wilhelm Schoner\",\"doi\":\"10.1016/0889-1605(89)90013-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Purified membrane-bound Na,K-ATPase incubated with cobalt-tetrammine-ATP [Co(NH<sub>3</sub>)<sub>4</sub>ATP], which is a stable MgATP complex analog, shows two new types of membrane crystals, a new p21 form and a p4 form. The building blocks of the crystalline arrays correspond to (<em>αβ</em>)<sub>2</sub> dimers of the enzyme protein suggesting that α-α interaction may be important in the pumping process.</p></div>\",\"PeriodicalId\":77743,\"journal\":{\"name\":\"Journal of ultrastructure and molecular structure research\",\"volume\":\"102 3\",\"pages\":\"Pages 189-195\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1989-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0889-1605(89)90013-X\",\"citationCount\":\"24\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of ultrastructure and molecular structure research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/088916058990013X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of ultrastructure and molecular structure research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/088916058990013X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 24
摘要
纯化的膜结合Na, k - atp酶与钴-四胺- atp [Co(NH3)4ATP](一种稳定的MgATP复合物类似物)一起培养，显示出两种新的膜晶体，一种新的p21形式和p4形式。晶体阵列的构建块对应于酶蛋白的(αβ)2二聚体，表明α-α相互作用可能在泵送过程中很重要。
Two-dimensional crystalline arrays of Na,K-ATPase with new subunit interactions induced by cobalt-tetrammine-ATP
Purified membrane-bound Na,K-ATPase incubated with cobalt-tetrammine-ATP [Co(NH3)4ATP], which is a stable MgATP complex analog, shows two new types of membrane crystals, a new p21 form and a p4 form. The building blocks of the crystalline arrays correspond to (αβ)2 dimers of the enzyme protein suggesting that α-α interaction may be important in the pumping process.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
