An electron microscopic study of cauliflower mosaic virus-induced viroplasms: Unusual structures within the viroplasm matrix with possible functional significance in the viral replication cycle

Abstract

A detailed ultrastructural study has been performed on viroplasms induced by two Cauliflower mosaic virus (CaMV) strains. Thin-sectioning and negative-staining electron microscopy techniques were both used. Examination of purified viroplasms by negative staining has revealed additional details in their ultrastructure. This analysis has consistently revealed a unique, previously undescribed, arc-shaped structure within the viroplasm matrix. Its presence is discussed in relation to a possible functional significance in the CaMV replication cycle.