Zentralblatt fur Mikrobiologie最新文献

{"title":"Studies on the refrigerated storage of wheat (Triticum aestivum). 2. Ergosterol, xanthomegnin, viomellein and brevianamide A after inoculation with Penicillium viridicatum.","authors":"H M Müller,&nbsp;A Boley","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Wheat seed was adjusted to 18, 20, 22, 24 and 26% moisture content (m.c.), and stored for 240 days at 4 or 10 degrees C following inoculation with a strain of Penicillium viridicatum producing the toxins, xanthomegnin (XA), viomellein (VIO), and brevianamide A (BA). Wheat kernels were not sterilized before inoculation. The concentration of ergosterol (ERG), a chemical indicator of fungal biomass, remained constant at 18% m.c./4 degrees C, but increased under the other conditions. The time before a detectable increase of ERG concentration was higher and the rate of ERG production lower with decreasing moisture content and temperature. XA and BA were produced at both temperatures at 20-26% m.c., VIO was produced at 22-26% m.c./4 degrees C and 20-26% m.c./10 degrees C. The results suggest or indicate that the onset of XA, VIO and BA production (detection limits: 10, 15, and 0.1 micrograms/kg, respectively) coincided with the onset of ERG production. Maximum toxin contents were lower with decreasing moisture content at both temperatures, but were similar at 4 and 10 degrees C at 22-26% m.c. It is concluded that wheat contaminated with P. viridicatum should not be stored beyond the onset of ergosterol production; maximum storage periods are recommended.</p>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 6","pages":"419-31"},"PeriodicalIF":0.0,"publicationDate":"1993-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19227072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of different serological methods for the detection of the fire blight pathogen, Erwinia amylovora (burrill) winslow et al.","authors":"R. Zielke ,&nbsp;A. Schmidt ,&nbsp;K. Naumann","doi":"10.1016/S0232-4393(11)80303-3","DOIUrl":"10.1016/S0232-4393(11)80303-3","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>Three serological methods (slide agglutination test for colonies isolated by a semiselective medium (Hahn agar), immunofluorescence technique (IFT) and enzyme-linked immunosorbent assay (DAS-ELISA) were compared for detecting <em>Erwinia amylovora</em>, the causal agent for fireblight, in fluids resp. on twigs contaminated and stored up for some days and by different temperatures.</p></span></li><li><span>2.</span><span><p>By all techniques the causal agent could be surely detected in concentrations of 10⁵ cells/ml; but by isolation on the semiselective agar medium (Hahn agar), still 10² cells/ml of <em>E. amylovora</em> were to find out easily. In many cases isolates of <em>E. herbicola</em>, a frequent saprophytic inhabitant of plant surfaces, showed cross reactions with antisera against <em>E. amylovora</em>, if agglutination test was used, but such reactions could not be completely eliminated by employing the IFT, too. However, in ELISA cross reactions with <em>E. herbicola</em> strains were not observed.</p></span></li><li><span>3.</span><span><p>In all experiments the results of IFT and DAS-ELISA essentially agreed.</p></span></li><li><span>4.</span><span><p>After few days of storing by different temperatures a decrease of the cell concentration in suspensions of E. amylovora could be demonstrated by the isolation method and by IFT. By short-time storing of suspensions (24 h) preservation by 22°C (laboratory) or 4 to 6°C (refrigerator) induced smaller losses of cell concentration than freezing (−21°C). By storing longer than 24 hours in samples preserved in a refrigerator (4 to 6°C) the slightest reduction of pathogen density could be stated.</p></span></li><li><span>5.</span><span><p>In contrast to, on twigs contaminated cell populations of the causal agent persisted without diminishing.</p></span></li></ul></div><div><p></p><ul><li><span>1.</span><span><p>In den vorliegenden Untersuchungen werden 3 serologische Verfahren (Objektträgeragglutination mit Bakterienkulturen von Isolierungsplatten (Hahn-Agar), Immunfluoreszenz, (DAS-ELISA) zum Nachweis von <em>Erwinia amylovora</em> in Flüssigmedien bzw. auf Zweigproben nach unterschiedlicher Aufbewahrungszeit/ -temperatur vergleichend geprüft.</p></span></li><li><span>2.</span><span><p>Mit den gewählten Verfahren konnte der Feuerbranderreger eindeutig nachgewiesen werden, wenn mindestens 1 × 10⁵ Bakterien pro ml Suspension vorlagen. Kreuzreaktionen mit <em>E. herbicola</em> traten in der Agglutination auf, konnten sich aber auch in der Immunfluoreszenztechnik mitunter störend bemerkbar machen. Mittels Isolierung auf Agarplatten waren noch 1 × 10² Zellen/ml gut zu erfassen.</p></span></li><li><span>3.</span><span><p>In allen gewählten Varianten zeigten der Immunfluoreszenztest und der ELISA im wesentlichen übereinstimmende Ergebnisse.</p></span></li><li><span>4.</span><span><p>Bei Aufbewahrung der Bakteriens","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 6","pages":"Pages 379-391"},"PeriodicalIF":0.0,"publicationDate":"1993-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80303-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56546491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of potassium sorbate on growth and penicillic acid production by Aspergillus ochraceus and Penicillium aurantiogriseum.","authors":"S. Garza, R. Canela, I. Viñas, V. Sanchis","doi":"10.1016/S0232-4393(11)80124-1","DOIUrl":"https://doi.org/10.1016/S0232-4393(11)80124-1","url":null,"abstract":"","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"256 1","pages":"343-50"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73106901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of variety and environmental factors on the phyto-effectivity of bacterial inoculations in peas","authors":"Gisela Höflich","doi":"10.1016/S0232-4393(11)80120-4","DOIUrl":"10.1016/S0232-4393(11)80120-4","url":null,"abstract":"<div><p>Of 171 strains of <em>Rhizobium leguminosarwn</em>, three strains repeatedly caused increased pea yields in pot and field experiments. A combined inoculation with all three strains did not give better results than single inoculations. However, a positive effect was found when combining the strains with a strain of not host-specific, phytohormone forming <em>Rhizobium</em>.</p><p>The effects of the inoculation are specific for the variety, but not for the growth type. After Inoculation with effective <em>Rhizobium</em>-strains, most varieties showed increased length and branching of roots, dry matter of roots and shoots and seed yield. One variety showed a negative reaction on the inoculation.</p><p>Optimal plant development and photosynthetic efficiency are important prerequisites for positive effects of the inoculation.</p><p>They can be varied by light intensity, soil humidity and temperature. Due to an increased infestation with soil borne plant pathogens and stronger water stress, the certainty of a continuous effect until maturity is lower under field conditions on loamy sand than on loess soils.</p></div><div><p>Von 171 <em>Rhizobium leguminosarum</em> Stämmen bewirkten 3 wiederholt Kornmehrerträge bei Erbsen in Gefäß- und Feldversuchen. Eine kombinierte Inokulation dieser drei Stämme verbesserte die Wirkung der Einzelinokulationen nicht. Positive Kombinationswirkungen zeichneten sich dagegen mit einem nichtwirts-pflanzenspezifischen phytohormonbildenden <em>Rhizobium</em>-Stamm ab.</p><p>Die Inokulationswirkungen sind sorten- aber nicht wuchstyp-spezifisch. Die meisten Sorten reagierten nach Inokulation effektiver <em>Rhizobium</em>-Stämme mit erhöhter Wurzellänge, Wurzelverzweigung, Wurzel- und Sproßtrockenmasse und höherem Kornertrag. Bei einer Sorte wirkte sich die Inokulation negativ aus.</p><p>Optimale Pflanzenentwicklung und Photosyntheseleistung sind wichtige Voraussetzungen für positive Inokulationswirkungen. Sie können durch Lichtintensität, Bodenfeuchte und Temperatur variiert werden. Unter Feldbedingungen ist die Wirkungssicherheit bis zur Kornreife auf lehmigem Sandboden, bedingt durch höheren Befall mit bodenbürtigen Schaderregern und höherem Trockenstreß, geringer als auf Löß-Schwarzerde.</p></div>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 5","pages":"Pages 315-324"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80120-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56537857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Filamentous fungi and mycotoxin detected in coconut","authors":"A.A. Zohri ,&nbsp;Sabah M. Saber","doi":"10.1016/S0232-4393(11)80121-6","DOIUrl":"https://doi.org/10.1016/S0232-4393(11)80121-6","url":null,"abstract":"<div><p>Fifty-nine species and one variety belonging to 25 genera of fungi were isolated from 25 coconut samples on glucose-Czapek's (25 genera and 55 species + 1 variety) and dichloran-glycerol (8 genera and 32 species + 1 variety) agar media at 28 °C. The common fungi on both media used were <em>Aspergillus flavus, A. niger, Penicillium chrysogenum</em> and <em>Cladosporium cladosporioides</em>. On glucose-Czapek's agar, <em>A. flavus</em> var. <em>columnaris, P. oxalicum, Alternaria alternata, Rhizopus stolonifer</em> and <em>Trichoderma hamatum</em> were recorded as common fungi while <em>A. sydowii</em> and <em>Eurotium chevalieri</em> were isolated with high frequency only on dichloran-glycerol medium. Chromatographic analysis of the chloroform extracts of the coconut samples revealed that 5 out of 25 samples tested were naturally contaminated with aflatoxin B₁ (15–25 μg/kg) and 3 samples contaminated with ochratoxin A (50–205 μg/kg).</p></div><div><p>59 Arten und 1 Varietät, die 25 Pilzgattungen angehörten, wurden aus 25 Kokosnuß-Proben auf Glucose-Czapek-Agar (25 Gattungen, 55 Arten + 1 Varietät) sowie Dichloran-Glycerol-Agar (8 Gattungen, 32 Arten + 1 Varietät) bei 28 °C isoliert. Die verbreitetsten Pilze in beiden Medien waren <em>Aspergillus flavus, A. niger, Penicillium chrysogenum</em> und <em>Cladosporium cladosporioides</em>. Auf Glucose-Czapek-Agar wurden noch häufig <em>A. flavus</em> var. <em>columnaris, P. oxalicum, Alternaria alternata, Rhizopus stolonifer</em> und <em>Trichoderma hamatum</em> gefunden, während <em>A. sydowii</em> und <em>Eurotium chevalieri</em> mit großer Häufigkeit nur in dem Dichloran-Glycerol-Medium isoliert wurden. Die chromatografische Analyse der Chloroform-Extrakte der Kokosnußproben ergab, daß 5 von 25 Proben natürlicherweise mit Aflatoxin B₁ (15–25 μg/kg) und 3 Proben mti Ochratoxin A (50–205 μg/kg) kontaminiert waren.</p></div>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 5","pages":"Pages 325-332"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80121-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91686323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of seed dressing on rhizosphere microflora of legumes I. biotic relations","authors":"O. Stefaniak ,&nbsp;W. Ślizak ,&nbsp;W. Piotrowski","doi":"10.1016/S0232-4393(11)80126-5","DOIUrl":"10.1016/S0232-4393(11)80126-5","url":null,"abstract":"<div><p>In a micro-plot experiment the effect of a six fungicides (Funaben T-suspended, Funaben T-liquid, Tiprosal, Dithane M-45, Infutox and Oftanol T) used as seed-dressings on the growth of microflora of white lupine, horse-bean and pea rhizospheres were evaluated. Concentration of all preparations was similar to that recommended in agriculture.</p><p>It has been shown that fungicides applied in this study influenced population of bacteria (B), actinomycetes (A) and fungi (F) as well as the quantitative relations among these organisms: (B + A)/F. Such the effect was different and depended not only on the kind of preparation but also on the species of plant and the stage of their growth. The most drastic and the most unprofitable changes have been observed in lupine rhizosphere after treatment with Tiprosal and liquid Funaben T.</p></div><div><p>In einem Kleinfeldversuch wurde der Einfluß einer Saatgutbeizung bei sechs Fungiziden auf die Entwicklung der Mikroflora in der Rhizosphäre der weißen Lupine, Pferdebohne und Erbse untersucht. Die Präparate wurden in einer für die landwirtschaftliche Praxis empfohlenen Konzentration angewendet.</p><p>Die Fungizide beeinflußten wesentlich die Menge der Bakterien (B), der Actinomyceten (A) und Pilze (F) sowie die Mengenverhältnisse unter diesen Organismen: (B + A)/F. Dieser Einfluß war jedoch unterschiedlich stark und hing nicht nur von der Art des Präparats, sondern auch von der Pflanzengattung und der Phase ihrer Entwicklung ab. Die stärksten und die am längsten dauernden Veränderungen der Mengenverhältnisse wurden in der Rhizosphäre von Lupine unter dem Einfluß von Tiprosal und Funaben T-flüssig beobachtet.</p></div>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 5","pages":"Pages 357-364"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80126-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56537938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"12th workshop on macro and trace elements in Jena","authors":"W. Merbach ,&nbsp;E. Reining","doi":"10.1016/S0232-4393(11)80129-0","DOIUrl":"10.1016/S0232-4393(11)80129-0","url":null,"abstract":"","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 5","pages":"Page 375"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80129-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56538638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Filamentous fungi and mycotoxin detected in coconut.","authors":"A A Zohri,&nbsp;S M Saber","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fifty-nine species and one variety belonging to 25 genera of fungi were isolated from 25 coconut samples on glucose-Czapek's (25 genera and 55 species + 1 variety) and dichloran-glycerol (8 genera and 32 species + 1 variety) agar media at 28 degrees C. The common fungi on both media used were Aspergillus flavus, A. niger, Penicillium chrysogenum and Cladosporium cladosporioides. On glucose-Czapek's agar, A. flavus var. columnaris, P. oxalicum, Alternaria alternata, Rhizopus stolonifer and Trichoderma hamatum were recorded as common fungi while A. sydowii and Eurotium chevalieri were isolated with high frequency only on dichloranglycerol medium. Chromatographic analysis of the chloroform extracts of the coconut samples revealed that 5 out of 25 samples tested were naturally contaminated with aflatoxin B1 (15-25 micrograms/kg) and 3 samples contaminated with ochratoxin A (50-205 micrograms/kg).</p>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 5","pages":"325-32"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19202626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of potassium sorbate on growth and penicillic acid production by Aspergillus ochraceus and Penicillium aurantiogriseum.","authors":"S Garza,&nbsp;R Canela,&nbsp;I Viñas,&nbsp;V Sanchis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of potassium sorbate on the growth and penicillin acid production of Aspergillus ochraceus and Penicillium aurantiogriseum was studied. Yeast extract sucrose (YES) broth at initial pH values of 5.5 or 7.0, and containing different concentrations of potassium sorbate was inoculated with fungal spores and incubated for 35 days at 28 degrees C. In all cases, although the pH changes in sorbate-containing media were delayed, patterns were similar to those of the cultures without sorbate. This was most evident when the highest concentration of potassium sorbate was used. Potassium sorbate also inhibited mycelial growth. Penicillic acid production was initially delayed by the presence of potassium sorbate but this inhibition was eventually overcome (30-35 days) and penicillic acid levels at the end of the experiment were similar or higher then the controls. Generally cultures growing in culture media at an initial pH of 5.5 produced less toxin than those growing at pH 7.0.</p>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 5","pages":"343-50"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19202627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of date products in production of oxytetracycline by Streptomyces rimosus","authors":"A.A. Abou-Zeid ,&nbsp;N.A. Baeshin ,&nbsp;A.O. Baghlaf","doi":"10.1016/S0232-4393(11)80122-8","DOIUrl":"10.1016/S0232-4393(11)80122-8","url":null,"abstract":"<div><p>The components of the synthetic fermentation medium, especially carbon, nitrogen, and mineral sources were replaced by Barni date-coat (fruit-flesh) sugar extract, date-seed hydrolysate, date-seed lipid, and date-seed ash. Date-coat sugar extract was rich in sugars; while date-seed hydrolysate contained more nitrogen sources. Both date sources were utilized by the organism to give high titres of the antibiotic. The deficiency in nitrogen was overcome by the addition of urea. It was found that date-seed ash in concentration of 0.5 mg/ml could replace MgSO₄, MnSO₄, FeSO₄, and ZnSO₄ when depleted from the medium.</p><p>Combination of date sources (date-coat sugar extract, date-seed hydrolysate, date-seed lipid, and date-seed ash) was investigated to formulate a natural medium for formation of Oxytetracycline. The natural medium contained date sources was a convenient substrate for the biosynthesis of Oxytetracycline.</p></div><div><p>Die Komponenten eines synthetischen Fermentationsmediums, besonders C, N und Mineralquellen, wurden durch Barni Dattelhüllen-(Fruchtfleisch-)Zuckerextrakt, Dattelsamen-Hydrolysat, Dattelsamen-Lipide und Dattelsamenasche ersetzt. Der Zuckerextrakt der Dattelhüllen war reich an Zuckern, während Dattelsamen-Hydrolysat mehr N-Quellen enthält. Beide Dattelquellen wurden durch den Mikroorganismus verwertet und ergaben hohe Titer des Antibiotikums. Der Mangel an Stickstoff wurde durch Hinzufügen von Harnstoff überwunden. Weiterhin wurde gefunden, daß die Dattelsamenasche in einer Konzentration von 0,5 mg/ml MgSO₄, MnSO₄, FeSO₄und ZnSO₄ ersetzt.</p><p>Die Kombination aller Dattelquellen (Dattelhüllen-Zuckerextrakt, Samenlipide und Samenasche) wurde näher untersucht, um ein natürliches, geeignetes Medium für die Oxytetracyclin-Biosynthese zu erhalten.</p></div>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 5","pages":"Pages 333-341"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80122-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56537879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}