Theodore A Slotkin, Ashley Stadler, Samantha Skavicus, Jennifer Card, Jonathan Ruff, Edward D Levin, Frederic J Seidler
{"title":"Is There a Critical Period for the Developmental Neurotoxicity of Low-Level Tobacco Smoke Exposure?","authors":"Theodore A Slotkin, Ashley Stadler, Samantha Skavicus, Jennifer Card, Jonathan Ruff, Edward D Levin, Frederic J Seidler","doi":"10.1093/toxsci/kfw180","DOIUrl":"10.1093/toxsci/kfw180","url":null,"abstract":"<p><p>Secondhand tobacco smoke exposure in pregnancy increases the risk of neurodevelopmental disorders. We evaluated in rats whether there is a critical period during which tobacco smoke extract (TSE) affects the development of acetylcholine and serotonin systems, prominent targets for adverse effects of nicotine and tobacco smoke. We simulated secondhand smoke exposure by administering TSE so as to produce nicotine concentrations one-tenth those in active smoking, with 3 distinct, 10-day windows: premating, early gestation or late gestation. We conducted longitudinal evaluations in multiple brain regions, starting in early adolescence (postnatal day 30) and continued to full adulthood (day 150). TSE exposure in any of the 3 windows impaired presynaptic cholinergic activity, exacerbated by a decrement in nicotinic cholinergic receptor concentrations. Although the adverse effects were seen for all 3 treatment windows, there was a distinct progression, with lowest sensitivity for premating exposure and higher sensitivity for gestational exposures. Serotonin receptors were also reduced by TSE exposure with the same profile: little effect with premating exposure, intermediate effect with early gestational exposure and large effect with late gestational exposure. As serotonergic circuits can offset the neurobehavioral impact of cholinergic deficits, these receptor changes were maladaptive. Thus, there is no single 'critical period' for effects of low-level tobacco smoke but there is differential sensitivity dependent upon the developmental stage at the time of exposure. Our findings reinforce the need to avoid secondhand smoke exposure not only during pregnancy, but also in the period prior to conception, or generally for women of childbearing age.</p>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"137 1","pages":"75-84"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78513412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Four Pesticides on Aflatoxin Production by Aspergillus flavus IMI 89717","authors":"I.A. El-Kady , S.S.M. El-Maraghy , A.Y. Abdel-Mallek , H.A.H. Hasan","doi":"10.1016/S0232-4393(11)80219-2","DOIUrl":"10.1016/S0232-4393(11)80219-2","url":null,"abstract":"<div><p>The effect of three fungicides (Vitavax-Captan, Rizolex-T and Sumisclex) and one insecticide (Actellic), when incorporated into liquid medium or applied to corn grains and sunflower seeds, on the production of aflatoxin by <em>A.flavus</em> IMI 89717 was tested. In liquid medium, aflatoxin production was inhibited by 27%, 82%, 100% and 100% when Vitavax-Captan was added at 10, 25, 50 and 100ppm, respectively. Sumisclex reduced to some extent the production of total aflatoxin, while Rizolex-T and Actellic did not affect quantitatively the mycotoxin production. Rizolex-T proved to be the the most effective pesticides tested on aflatoxin production on both corn-grains and sunflower seeds, while Vitavax-Captan was the second most effective pesticide. Both Sumisclex and Actellic did not inhibit aflatoxin production on either corn grains or sunflower seeds at the levels tested.</p></div><div><p>Der Effekt dreier Fungizide (Vitavax-Caplan, Rizolex-T und Sumisclex) und einem Insektizid (Actellic) auf die Aflatoxinbildung bei <em>Aspergillus flavus</em> IMI 89717 wurde bei Flüssigmediumzugabe bzw. Zugabe zu Getreidekörnern und Sonnenblumensamen untersucht. Im Flüssigmedium wurde die Aflatoxinbildung gehemmt, wenn Vitavax-Captan in Konzentrationen von 10, 25, 50 und 100 ppm zugefügt wurde (jeweils in % 27, 82, 100, 100). Sumisclex verminderte die Bildung von Aflatoxin, während Rizolex-T und Actellin die Mycotoxinbildung quantitativ nicht beeinflußten. Rizolex-T war das effektivste Pestizid, welches die Aflatoxinbildung bei Samenanwendung beeinflußte, gefolgt von Vitavax-Captan. Sumisclex und Actellin dagegen zeigten keine Hemmung der Aflatoxinbildung bei Samenanwendung unter den verwendeten Konzentrationen.</p></div>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 8","pages":"Pages 549-557"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80219-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76418667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I A el-Kady, S S el-Maraghy, A Y Abdel-Mallek, H A Hasan
{"title":"Effect of four pesticides on aflatoxin production by Aspergillus flavus IMI 89717.","authors":"I A el-Kady, S S el-Maraghy, A Y Abdel-Mallek, H A Hasan","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of three fungicides (Vitavax-Captan, Rizolex-T and Sumisclex) and one insecticide (Actellic), when incorporated into liquid medium or applied to corn grains and sunflower seeds, on the production of aflatoxin by A. flavus IMI 89717 was tested. In liquid medium, aflatoxin production was inhibited by 27%, 82%, 100% and 100% when Vitavax-Captan was added at 10, 25, 50 and 100 ppm, respectively. Sumisclex reduced to some extent the production of total aflatoxin, while Rizolex-T and Actellic did not affect quantitatively the mycotoxin production. Rizolex-T proved to be the most effective pesticides tested on aflatoxin production on both corn-grains and sunflower seeds, while Vitavax-Captan was the second most effective pesticide. Both Sumisclex and Actellic did not inhibit aflatoxin production on either corn grains or sunflower seeds at the levels tested.</p>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 8","pages":"549-57"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19290525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D.K. Maheshwari , M. Gupta, R. Sawhney, A. Khandelwal
{"title":"Dual Behariour of Carbaryl and 2,4-Dichlorophenoxyacetic Acid in Rhizobium leguminosarum 2005 under Explanta Conditions","authors":"D.K. Maheshwari , M. Gupta, R. Sawhney, A. Khandelwal","doi":"10.1016/S0232-4393(11)80224-6","DOIUrl":"10.1016/S0232-4393(11)80224-6","url":null,"abstract":"<div><p>Studies have shown that major pesticide (carbaryl) and herbicide (2,4-D) at higher concentrations (50 μg/ml) were inhibitory to the <em>Rhizobium</em> growth but they stimulated oxygen uptake in a concentration dependent manner. Extremely low concentration of carbaryl (1 μg/ml) and 2,4-D (5 μg/ml) treated culture showed enhanced growth. Nitrate Reductase (NR) and Nitrite Reductase (NiR) activities increase in the presence of 125 μg/ml concentration of carbaryl (1-napthyl methyl carbamate) and 300 μg/ml.of 2,4-D (2,4-Dichlorophenoxy acetic acid). Further increase in concentration led to decline in NR and NiR activities. Dual behaviour of these chemicals may be due to their action as electron donors and thereby causing alteration through oxidative metabolic pathway. It is suggested that besides pesticidal and herbicidal action, carbaryl and 2,4-D may also be used as well to harness the beneficial effects of nitrogen fixing organisms.</p></div><div><p>Die Untersuchungen haben gezeigt, daß die verbreiteten Pestizide Carbaryl und 2,4-D in höheren Dosen (50 μg/ml) auf das Wachstum von <em>Rhizobium</em> hemmend wirken, jedoch die Sauerstoffaufnahme abhängig von der Konzentrationshöhe stimulieren. Mit extrem niedrigen Konzentrationen von Carbaryl (1 ug/ml) und 2,4-D (5 μg/ml) behandelte Kulturen zeigen ein erhöhtes Wachstum. Nitratreduktase (NR)- und Nitritreduktase (NiR)-Aktivitäten waren bei einer Konzentration von 125 μg/ml Carbaryl (1-Napthyl-Methyl-Carbamat) bzw. von 300 μg/ml 2,4-D (2,4-Dichlorphenoxiessigsäure) erhöht. Eine weitere Konzentrationserhöhung führte zu einer Abnahme der NR- und NiR-Aktivitäten. Das duale Verhalten dieser Chemikalien dürfte auf ihre Wirkungsweise als Elektronendonator zurückzuführen sein und dabei Veränderungen im oxidativen Stoffwechsel verursachen. Es wird gezeigt, daß neben der Pestiziden und herbiziden Wirkung Carbaryl und 2,4-D ebenfalls als Begrenzer eines günstigen Effekts der N-Fixierung wirken können.</p></div>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 8","pages":"Pages 588-592"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80224-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56542966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fungitoxic Effect of Different Substances from Solenostemma argel (Del) Hayne on Some Shoot Surface Fungi","authors":"F.K. Abd El-Hady , S.A. Ouf","doi":"10.1016/S0232-4393(11)80226-X","DOIUrl":"10.1016/S0232-4393(11)80226-X","url":null,"abstract":"<div><p>Thirty five fungal species belonging to sixteen genera were isolated from the shoot surface of <em>S. argel. Aspergillus</em> exhibited uncomparable counts reaching 74.9% of the total counts. Of 13 isolated <em>Aspergillus</em> spp., <em>A. humicola, A. niger, A. sulphureous</em> and <em>A. versicolor</em> were the major. <em>Aspergillus</em> was followed by <em>Cladosporium, Alternaria, Penicillium</em> and <em>Curvularia</em>. The fungitoxic activity of extracts from the target plant against spore germination of <em>A. Candidus</em> and <em>P. lanosum</em> showed toxicity variabilities. Petroleum ether and ether extracts were ineffective as fungitoxicant. The lower concentrations of the other extracts were stimulative to <em>A. Candidus</em> and suppressive to <em>P. lanosum</em>. The reached result at this concentration was coupled with the distribution pattern of the two fungi on shoot surface. The effective extracts were inhibitory at higher doses with maximum inhibition manifested by CH<sub>2</sub>Cl<sub>2</sub>/MeOH fraction. Chemical characterization of this fraction using paper chromatography, U.V. and spectral data revealed the presence of five flavonoid glycosides of which Kaempferol 3-glucoside and Kaempferol 3-diglucoside were identified.</p><p>Although the sole application of the isolated flavonoids was inhibitory to spore germination of both fungi, however the combined presence appears to be stimulative for the spore germination.</p></div><div><p>Von der Sproßoberfläche von <em>Solenostemma argel</em> wurden 35 Pilzspecies aus 16 Gattungen isoliert. Aspergillen standen mit 74,9% weit an der Spitze der Keimzahlen. Von den 13 isolierten Species dieser Gattung waren <em>Aspergillus humicola, A. niger, A. sulphureous</em> and <em>A. versicolor</em> die häufigsten. Nach der Häufigkeit folgten Vertreter der Gattungen <em>Cladosporium, Alternaria, Penicillium</em> and <em>Curvularia</em>. Verschiedene Extrakte der untersuchten Pflanze zeigten unterschiedliche toxische Wirkung auf die Sporenkeimung von <em>Aspergillus Candidus</em> und <em>Penicillium lanosum</em>. Petrolether und Etherextrakte waren unwirksam. Andere Extrakte stimulierten in niedriger Konzentration <em>Aspergillus candidus</em>, aber hemmten <em>Penicillium lanosum</em>. Diese Ergebnisse gehen konform mit dem Vorkommen der beiden Pilzarten auf der Sproßoberfläche. Die wirksamen Extrakte wirkten in höheren Dosen hemmend, wobei die CH<sub>2</sub>Cl<sub>2</sub>/MeOH-Fraktion das Maximum erreichte. Eine chemische Charakterisierung dieser Fraktion mittels Papierchromatografie, UV und Spektralanalyse zeigte das Vorkommen von 5 Flavonoid-Glycosiden, von denen Kaempferol-3-glucosid und Kaempferol-3-diglucosid identifiziert wurden.</p><p>Obwohl die Einzelanwendung der isolierten Flavonoide die Sporenkeimung der beiden Testpilze hemmte, scheint die gemeinsame Anwendung die Sporenkeimung zu stimulieren.</p></div>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 8","pages":"Pages 598-607"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80226-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56543048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M.A. Abdel-Sater , A.M. Khallil , M.A. Ismail , S.A. Ahmed
{"title":"Effect of Paraquat and Ametryne on Soil, Root and Leaf-Surfaces Fungi of Solanum tuberosum L.","authors":"M.A. Abdel-Sater , A.M. Khallil , M.A. Ismail , S.A. Ahmed","doi":"10.1016/S0232-4393(11)80220-9","DOIUrl":"10.1016/S0232-4393(11)80220-9","url":null,"abstract":"<div><p>The effects of the herbicides (Ametryne and Paraquat) on soil, root and leaf-surfaces fungi (both zoosporic and terrestrial) inhabiting potato (<em>Solanum tuberosum</em> L.) were generally inconsistent. They were almost toxic to the total count and the toxicity extended till the end of experimentation period (4 weeks). Most of the fungal species were also poisoned at certain periods and doses. Some fungal species showed two reactions the promotive one in shorter periods and the inhibitory response after longer periods.</p><p>When the herbicides were incorporated with liquid medium, most of the tested fungal species were inhibited even at the low doses. <em>Emericella nidulans</em> and <em>Trichoderma harzianum</em> were the most resistant species. Some species were resistant to Paraquat but sensitive to Ametryne and vice versa.</p></div><div><p>Die Wirkung zweier Herbizide (Ametryn und Paraquat) auf Boden, Wurzel und Blattoberflächen besiedelnde Pilz von Kartoffeln war im allgemeinen unterschiedlich. Sie war meistens auf die Gesamtzahl toxisch und wirkte bis zum Ende der Experimentalzeit (4 Wochen). Die meisten der Pilzarten wurden in bestimmten Perioden und bei bestimmten Konzentrationen negativ beeinflußt. Einige Pilze zeigten zwei unterschiedliche Reaktionen: Eine kurzzeitige Vermehrung und eine Hemmung nach längerer Zeit.</p><p>Bei Verabreichung der Herbizide zusammen mit dem flüssigen Medium wurde die Mehrzahl der Pilzarten auch bei niedrigen Konzentrationen gehemmt. <em>Emericella nidulans</em> und <em>Trichoderma harzianum</em> waren die resistentesten Arten. Einige Arten waren zwar resistent gegenüber Paraquat, aber empfindlich gegenüber Ametryn, bei anderen war es umgekehrt.</p></div>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 8","pages":"Pages 558-569"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80220-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56542369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inhibitory Effect of Spice Oils on Lipase and Mycotoxin Production","authors":"H.A.H. Hasan, A.-L.E. Mahmoud","doi":"10.1016/S0232-4393(11)80218-0","DOIUrl":"10.1016/S0232-4393(11)80218-0","url":null,"abstract":"<div><p>Five spice oils were tested for their inhibitory activity towards the growth, respiration, lipase and mycotoxin productions by <em>Aspergillus parasiticus</em> var. <em>globosus</em> IMI 120920 and <em>A. fumigatus</em>. Cumin, onion, garlic and clove oils completely inhibited sterigmatocystin production. Cumin, onion and clove oils significantly suppressed aflatoxin production. Most of tested oils exhibited considerable inhibition on mycelial growth. Incorporation of these oils in basal medium suppressed lipase production with variable degrees. Onion oil was the most effective one. All spice oils significantly reduced or completely suppressed CO<sub>2</sub> evolution of <em>A. fumigatus</em>, whereas CO<sub>2</sub> evolution of <em>A. parasiticus</em> was slightly enhanced by these oils except with cumin and clove. Our results suggest that the use of spice oils may offer some advantage in the prevention of mycotoxin production.</p></div><div><p>Es wurden 5 Gewürzöle auf ihren Hemmeffekt in bezug auf Wachstum, Atmung, Lipase- und Mycotoxinbildung bei <em>Aspergillus parasiticus</em> var. <em>globosus</em> IMI 120920 und bei <em>Aspergillus fumigatus</em> untersucht. Kreuzkümmel- und Zwiebelöle hemmten das Wachstum, die Lipase sowie die Bildung von Aflatoxin und Sterigmatocystin schon bei 250 ppm. Gewürznelken, Knoblauch und schwarzer Kümmel hemmten die getesteten Pilze bei 500 und 1000 ppm. Die CO<sub>2</sub>-Bildung wurde bei <em>A. fumigatus</em> gehemmt, bei <em>A. parasiticus</em> allerdings nur teilweise beeinflußt.</p></div>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 8","pages":"Pages 543-548"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80218-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85721393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mucor thermophilus spec. nov. and other Mucorales from India","authors":"R. Prakash , A.K. Sarbhoy","doi":"10.1016/S0232-4393(11)80216-7","DOIUrl":"10.1016/S0232-4393(11)80216-7","url":null,"abstract":"<div><p>Ninety-five isolates belonging to the order <em>Mucorales</em> were collected from the vicinity of Delhi. These were derived as pure sporulating forms from various substances viz., dung, soil and compost. In all, twenty-one different species of <em>Absidia, Cunninghamella, Mucor</em> and <em>Rhizopus</em> were identified with the help of available literature (Hesseltine and Ellis 1964, 1966, Inui et al. 1965, Schipper 1978, 1984, Baijal and Mehrotra 1980 and Schipper and Stalpers 1984). Out of these, five fungi were new to India. A new species of <em>Mucor</em> and <em>Rhizopus microsporus</em> var. <em>pseudochinensis</em> comb. nov. is described.</p></div><div><p>95 Isolate, die zur Ordnung der <em>Mucorales</em> gehörten, wurden aus der Umgebung von Delhi gesammelt. Sie wurden als reine Sporulationsformen von verschiedenen Substanzen erhalten, so aus Dung, Boden und Kompost. Dabei wurden mit Hilfe einschlägiger Literatur 21 verschiedene Arten von <em>Absidia, Cunninghamella, Mucor</em> und <em>Rhizopus</em> identifiziert. Von den isolierten Pilzen konnten fünf Neuentdeckungen in Indien festgestellt werden. Eine neue Art von <em>Mucor</em> wurde beschrieben und <em>Rhizopus microsporus</em> var. <em>pseudochinensis</em> konnte neu kombiniert werden (comb, nov.)</p></div>","PeriodicalId":77473,"journal":{"name":"Zentralblatt fur Mikrobiologie","volume":"148 8","pages":"Pages 531-534"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0232-4393(11)80216-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78895064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}