Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis最新文献

{"title":"[Determining free activity of cathepsin D in tissue homogenates after precipitation of cellular organelles in pH 5,0].","authors":"E Skrzydlewska,&nbsp;K Worowski,&nbsp;J Jurkowski,&nbsp;H Ostrowska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Homogenate is prepared in 0.25 M sucrose. Cellular organelles are precipitated by acidification to pH 5.0 and removed by centrifugation at 1500 x g during 30 minutes. In the obtained cytosol which does not contain lysosomes free activity of cathepsin D is determined.</p>","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"35-36 ","pages":"15-20"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13291046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Regressive changes in the placenta in early pregnancy in women with primary arterial hypertension].","authors":"W Lotocki,&nbsp;E Król,&nbsp;M Drozdzewicz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Morphological methods, in particular histochemical technique of secondary fluorescence according to Bertalanffy were used for the assessment of regressive changes of placenta. In women with primary arterial hypertension during early pregnancy fibrinoid masses in decidua, chorion, anchoring willi, villous plate, basal plate, villous trunks and terminal villi appear. These changes are followed from one side, by an atrophy of the blood vessels and connective tissue cells, and from the other, there increases the number of decidua giant cells, as well as of syncytium and cytotrophoblast. The areas of impaired blood supply contain giant cells and intervening foam cells. These cells according to the authors can be the forms of regressive changes exhibiting relative biological activity influencing trophic status of the placenta. Possible importance of the fibrinoid masses for the immunology of pregnancy which is developing under maternal morbid conditions has been discussed.</p>","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"35-36 ","pages":"71-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12890134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A simple method of synthesis of 3-acetyl-5-(D-arabino-tetrahydroxybutyl)-2-methylpyrrole].","authors":"A Rózański,&nbsp;K Bielawski,&nbsp;J Bołtryk,&nbsp;D Bartulewicz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The reaction of D-fructose with ammonium carbonate and 2,4-pentanedione leads in one step to 3-acetyl-5-(D-arabino-tetrahydroxybutyl)-2-methylpyrrole. This compound has been found to display anticancer activity.</p>","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"35-36 ","pages":"57-63"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13290304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of pesticides on selected biochemical parameters in the serum and liver of rats].","authors":"D Pawłowska,&nbsp;J Moniuszko-Jakoniuk,&nbsp;A Lukaszewicz-Hussain","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The experiments were carried out on male Wistar rats, weighing 200-250 g. The animals received oil or oily solutions of carbaryl, parathion-methyl and IPO62 in doses of 0.5, 0.1 or 0.02 LD50. 1, 2, 4 or 24 h after the administration of pesticides, the activities of ChE and blood glucose level, liver glycogen level, FFA and TG concentrations in serum and TG level in liver were estimated. Comparing the enzymatic changes in acute intoxications with carbaryl, parathion-methyl or IPO62, in the same doses, it was found that mechanisms of efficacy of those compounds had different trends. Changes in the activities of such parameters as ChE in serum, BGR, AspAT and AlAT in serum and liver and blood glucose level depend on the dose of pesticides and this effect is observed for IPO62, parathion-methyl and carbaryl.</p>","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"35-36 ","pages":"143-61"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13291045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of immunomodulating drugs on the release and activities of lysosomal proteinases of the liver of rats with ethanol poisoning].","authors":"E Skrzydlewska,&nbsp;L Chyczewski,&nbsp;K Worowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Increased activity of cathepsin A and D in the cytosol fraction and homogenate of the liver of rats intoxicated for 4 weeks with ethanol (0.6 g/100 g of the body weight) was found. The cytosol cathepsin A and D activities were unaffected under the influence of Levamisole and isoprinosine++. Encorton reduced the activity of both cathepsins in the cytosol fraction while it did not diminish their activities in the liver homogenates. Encorton, and to a markedly lesser degree, Levamisole and isoprinosine++ caused a regression of vacuolar degeneration and of necrotic lesions and an increase in the number of glycogen granules in the livers of ethanol-intoxicated rats.</p>","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"35-36 ","pages":"163-75"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12890136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of cysteine on protein metabolism in the liver of rats with ethanol-induced liver damage].","authors":"E Skrzydlewska,&nbsp;K Worowski,&nbsp;L Chyczewski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rats intoxicated with ethanol at the dose of 0.6 g/100 g of the body weight during 4 weeks were fed on standard diet and the one containing 0.125, 0.25 and 0.5% L-cysteine. Intoxication of rats fed standard food causes an increase in the activity of cathepsin D and gamma-glutamyl-transpeptidase in the liver and an increase in the activity of alanine aminotransferase and gamma-glutamyl-transpeptidase in the blood serum. Consuming by rats food containing small and medium quantity of cysteine causes normalization of the activity of all enzymes, whereas consuming food containing large quantity of cysteine does not give such effect.</p>","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"35-36 ","pages":"129-41"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13140748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[An optimal method of determining the level of cathepsin A in blood platelet homogenates].","authors":"H Ostrowska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Blood platelet cathepsin A hydrolyzes Cbz-Glu-Tyr most intensively at pH 5.0, it does so too, with Cbz-Glu-Phe, but to a smaller extent. Using the ninhydrin method for estimation of the released tyrosine++ or phenylalanine previous elimination of amino acids++ and low-molecular peptides is necessary as it is too including alpha-amine nitrogen released by platelet proteases from peptides and proteins.</p>","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"33-34 ","pages":"33-41"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14284796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of detergents on the solubilization of 5'-nucleotidase from the thyroid gland].","authors":"E Piotrowska,&nbsp;J Niedźwiecka,&nbsp;L Jaroszewicz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of Triton X-100, sodium deoxycholate and saponin upon the solubilization of 5'-nucleotidase, the membrane enzyme derived from pig thyroid was studied. Triton X-100 at the concentration of 0.1% did not cause enzyme solubilization, whereas at the concentration of 1.0% it caused only partial release of the former from the membranes. Saponin (1.0% concentration) brought about a marked (about threefold) increase in the enzyme activity which resulted from the exposing active loci of the enzyme, however it did not cause total solubilization of it. 1% sodium deoxycholate increased the enzyme activity by 5 times and also caused its almost total solubilization (over 90%). The results indicate the 5'-nucleotidase is strongly bound to the cell membranes and the non-ionic detergents like Triton X-100 and saponin do not fit for the solubilization of this enzyme.</p>","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"33-34 ","pages":"11-21"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13993776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[20-year cooperation between the I Department of Internal medicine of the District Hospital and the Department of Pathological Anatomy, Medical Academy, in Białystok (analysis of diagnostic consistency)].","authors":"J Fiedorowicz,&nbsp;I Jakubowska,&nbsp;W Pedich,&nbsp;L Zimnoch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Statistical analysis of 1421 deaths (98% of all who had died at the Ward) of patients during 1965-1984 was performed. The material was divided into 3 groups according to the age: up to 59 years, from 60 to 69 and over 70 years. Among those who had died men prevailed (55.1%), but both among women and men the age was over 70 years. In the 2nd decade of the analysed period the authors found gradual increase of death percentage among elderly people, that can be ascribed to \"geriatrization\" of the Ward and higher accessibility to the hospital for this group of patients. Statistical analysis which included a 10-year period (1975-1984) dealt with the causes of deaths according to the clinical and pathological recognition. There dominated deaths of cardiovascular diseases (59.4%), twice increased the percent of deaths due to neoplasia and diabetes complications. On the other hand, the proportion of decreased of heart infarction fell (from 33% to 16.9%) as did of cardiac defects (from 9.5% to 5.9%). This decrease was the effect of hospital structure changes, i.e.a creation of the Ward of Cardiology and Intensive Cardiological Care in 1981. During 1981-1984 the proportion of deaths of complications of arterial hypertension, mainly in the persons at very old age, increased threefold. In this group of age there dominated deaths of cardiac infarction and circulatory insufficiency. The authors made an analysis of the conformability of clinical diagnosis and the results of autopsy. Out of 1421 deaths in 1149 cases (80.8%) the results of autopsy fully agreed with clinical diagnosis. As partially conformable the clinical diagnosis was in 15.5%, and divergent in 3.7% of cases. The latter, according to the authors, resulted from an incomplete or unproper interpretation of diseases symptoms influenced by several objective factors. As could be expected, the highest proportion of divergent diagnosis was found in the group of the eldest patients afflicted with cardiovascular and malignant diseases.</p>","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"33-34 ","pages":"85-97"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14284092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Thyroid gland inositol-1-phosphate synthase (its purification and characteristics)].","authors":"H Stelmach,&nbsp;L Jaroszewicz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Pig thyroid myoinositol-phosphate synthase was purified about 30 times using ammonium sulphate fractionation and DEAE cellulose chromatography. The enzyme preparation showed the activity of more than 70 mU/mg of protein. A partially purified synthase is a very labile enzyme. Its activity showed optimum value at pH 7.0. This activity appeared to be controlled by NH4+, Na+, and Li+ ions. The biological role of thyroid synthase has been discussed.</p>","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"33-34 ","pages":"23-32"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14284794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}