[甲状腺肌醇-1-磷酸合成酶(其纯化及特性)]。

Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis Pub Date : 1988-01-01

H Stelmach, L Jaroszewicz

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摘要

猪甲状腺肌醇-磷酸合成酶采用硫酸铵分馏和DEAE纤维素层析纯化约30次。酶制剂对蛋白质的活性大于70 mU/mg。部分纯化的合酶是一种非常不稳定的酶。其活性在pH 7.0时达到最佳。这种活性似乎受NH4+、Na+和Li+离子的控制。本文讨论了甲状腺合酶的生物学作用。

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[Thyroid gland inositol-1-phosphate synthase (its purification and characteristics)].

Pig thyroid myoinositol-phosphate synthase was purified about 30 times using ammonium sulphate fractionation and DEAE cellulose chromatography. The enzyme preparation showed the activity of more than 70 mU/mg of protein. A partially purified synthase is a very labile enzyme. Its activity showed optimum value at pH 7.0. This activity appeared to be controlled by NH4+, Na+, and Li+ ions. The biological role of thyroid synthase has been discussed.

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Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis

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