[Determining free activity of cathepsin D in tissue homogenates after precipitation of cellular organelles in pH 5,0].

Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis Pub Date : 1990-01-01

E Skrzydlewska, K Worowski, J Jurkowski, H Ostrowska

引用次数: 0

Abstract

Homogenate is prepared in 0.25 M sucrose. Cellular organelles are precipitated by acidification to pH 5.0 and removed by centrifugation at 1500 x g during 30 minutes. In the obtained cytosol which does not contain lysosomes free activity of cathepsin D is determined.

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[测定pH值为5,0的细胞器沉淀后组织匀浆中组织蛋白酶D的游离活性]。

匀浆用0.25 M蔗糖配制。细胞器通过酸化沉淀至pH 5.0，并在1500 x g离心30分钟去除。在获得的不含溶酶体的细胞质中测定组织蛋白酶D的游离活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis

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