测定血小板匀浆中组织蛋白酶A水平的最佳方法。

Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis Pub Date : 1988-01-01

H Ostrowska

{"title":"测定血小板匀浆中组织蛋白酶A水平的最佳方法。","authors":"H Ostrowska","doi":"","DOIUrl":null,"url":null,"abstract":"Blood platelet cathepsin A hydrolyzes Cbz-Glu-Tyr most intensively at pH 5.0, it does so too, with Cbz-Glu-Phe, but to a smaller extent. Using the ninhydrin method for estimation of the released tyrosine++ or phenylalanine previous elimination of amino acids++ and low-molecular peptides is necessary as it is too including alpha-amine nitrogen released by platelet proteases from peptides and proteins.","PeriodicalId":77367,"journal":{"name":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","volume":"33-34 ","pages":"33-41"},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[An optimal method of determining the level of cathepsin A in blood platelet homogenates].\",\"authors\":\"H Ostrowska\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Blood platelet cathepsin A hydrolyzes Cbz-Glu-Tyr most intensively at pH 5.0, it does so too, with Cbz-Glu-Phe, but to a smaller extent. Using the ninhydrin method for estimation of the released tyrosine++ or phenylalanine previous elimination of amino acids++ and low-molecular peptides is necessary as it is too including alpha-amine nitrogen released by platelet proteases from peptides and proteins.\",\"PeriodicalId\":77367,\"journal\":{\"name\":\"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis\",\"volume\":\"33-34 \",\"pages\":\"33-41\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

血小板组织蛋白酶A在pH 5.0时对Cbz-Glu-Tyr的水解最强烈，对Cbz-Glu-Phe的水解也最强烈，但程度较小。使用ninhydrin法估计在氨基酸和低分子肽消除之前释放的酪氨酸++或苯丙氨酸是必要的，因为它也包括血小板蛋白酶从肽和蛋白质中释放的α胺氮。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

本刊更多论文

[An optimal method of determining the level of cathepsin A in blood platelet homogenates].

Blood platelet cathepsin A hydrolyzes Cbz-Glu-Tyr most intensively at pH 5.0, it does so too, with Cbz-Glu-Phe, but to a smaller extent. Using the ninhydrin method for estimation of the released tyrosine++ or phenylalanine previous elimination of amino acids++ and low-molecular peptides is necessary as it is too including alpha-amine nitrogen released by platelet proteases from peptides and proteins.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Roczniki Akademii Medycznej w Bialymstoku = Annales Academiae Medicae Bialostocensis

自引率

0.00%

发文量