American journal of translational research最新文献

{"title":"Association of dynamic changes in ASPECTS perfusion scores derived from dual-time-point multi-post-labeling delay arterial spin labeling with 90-day functional outcome in acute ischemic stroke.","authors":"Danwen Wang, Qingqing Li, Yichang Li","doi":"10.62347/POFV6679","DOIUrl":"10.62347/POFV6679","url":null,"abstract":"<p><strong>Objective: </strong>To investigate whether dynamic changes in Alberta Stroke Program Early CT Score (ASPECTS) perfusion scores derived from dual-time-point multi-post-labeling delay arterial spin labeling (multi-PLD ASL) are associated with 90-day functional outcome in patients with acute ischemic stroke (AIS).</p><p><strong>Methods: </strong>This single-center retrospective study included 464 patients with anterior-circulation AIS who underwent multi-PLD ASL at baseline and approximately 1 week after onset. Cerebral blood flow (CBF)-ASPECTS, cerebral blood volume (CBV)-ASPECTS, arterial transit time (ATT)-ASPECTS, relative perfusion values, and infarct core volume (ICV) were compared between favorable (modified Rankin Scale [mRS] 0-2) and unfavorable (mRS 3-6) outcome groups. Correlation and receiver operating characteristic (ROC) analyses were performed.</p><p><strong>Results: </strong>The favorable outcome group showed greater improvements in CBF-ASPECTS, CBV-ASPECTS, ATT-ASPECTS, relative cerebral blood flow (rCBF), and relative cerebral blood volume (rCBV), together with a greater reduction in relative arterial transit time (rATT). Increased ICV was more pronounced in the unfavorable outcome group. Dynamic ASL indicators were significantly correlated with 90-day mRS scores. Single indicators showed moderate discriminative ability, whereas combined models performed better. The clinical-imaging model showed the highest area under the curve (AUC) of 0.846.</p><p><strong>Conclusion: </strong>Short-term changes on dual-time-point multi-PLD ASL were associated with 90-day functional outcome in AIS. Dynamic ASPECTS perfusion scores may serve as an interpretable imaging supplement for prognosis assessment, but should be combined with clinical information.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"7092-7106"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture attenuates neuroinflammation by regulating the NLRP3 inflammasome pathway: an animal study.","authors":"Chengdong Yang","doi":"10.62347/RHNC9345","DOIUrl":"10.62347/RHNC9345","url":null,"abstract":"<p><strong>Objectives: </strong>To determine the effect of electroacupuncture at GV20 (Baihui) and ST36 (Zusanli) in reducing the LPS-induced neuroinflammation and cognitive deficit. To determine whether it was associated with reduced activation of the NLRP3 inflammasome-caspase-1-IL-1β/IL-18 signaling pathway in both male and female rats.</p><p><strong>Procedures: </strong>Ninety-four adult Sprague-Dawley rats (47 male, 47 female) were randomly assigned to four groups: Control (saline), LPS (5 mg/kg intraperitoneal), LPS+EA (LPS plus electroacupuncture at GV20/ST36, 2 Hz, 1 mA, 20 min/day, 7 days), and LPS+Sham Electroacupuncture (LPS plus sham electroacupuncture at non-acupoints). Outcomes included NLRP3 and caspase-1 p20 (western blot), IL-1β, IL-18, IL-6, TNF-α, IL-10 (ELISA), ROS, MDA, GSH (oxidative stress assays), Iba-1, and GFAP (immunohistochemistry), GSDMD-NT, NeuN, NF-κB p65, phospho-NF-κB p65, BDNF, CD68, iNOS, Arginase-1, and cognitive function (Morris water maze, Y-maze, novel object recognition).</p><p><strong>Results: </strong>LPS increased all inflammatory and oxidative stress markers and impaired cognitive versus Controls (P < 0.001). Electroacupuncture was associated with significant reductions compared to LPS (P < 0.001): NLRP3 (48% reduction), caspase-1 p20 (54%), IL-1β (34%), IL-18 (33%), IL-6 (49%), TNF-α (49%), ROS (40%), MDA (47%), Iba-1 (46%), GFAP (47%), GSDMD-NT (54%), NF-κB p65 (46%), p-NF-κB p65 (68%), CD68 (57%), iNOS (68%), while increasing IL-10 (98%) and GSH (60%), NeuN (77%), BDNF (116%), Arginase-1 (500%), and improving cognition (21-133%). Sham EA had no effect on any outcome (P > 0.05 vs. LPS). No significant sex differences were detected. Mediation analysis revealed that 84.4% of electroacupuncture's cognitive benefit was mediated through the NLRP3-related pathways.</p><p><strong>Conclusions: </strong>Electroacupuncture at GV20 and ST36 was associated with reduced LPS-induced neuroinflammation and cognitive impairment and these changes were statistically consistent with involvement of the NLRP3-caspase-1-IL-1β/IL-18 pathway, with no significant sex differences. These findings suggest electroacupuncture may be a promising strategy for NLRP3-associated neuroinflammatory disorders.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6591-6611"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of loading dose atorvastatin on postoperative cardiovascular events in elderly patients with acute myocardial infarction undergoing percutaneous coronary intervention.","authors":"Lijiao Zhang, Fangfang Zhong, Changqing Zhou, Yushu Sun, Chen Ye","doi":"10.62347/ONOI2544","DOIUrl":"10.62347/ONOI2544","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effects of loading-dose atorvastatin on cardiac function, lipid metabolism, inflammatory response, and cardiovascular events in elderly patients with acute myocardial infarction (AMI) undergoing percutaneous coronary intervention (PCI).</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 128 elderly patients with AMI (≥60 years) who underwent PCI. Patients were divided into a loading-dose group (group A; 40-80 mg loading dose, n=71) and a conventional-dose group (group B; 20 mg or without a loading dose, n=57). Cardiac functionindices, including cardiac output, left ventricular ejection fraction (LVEF), left ventricular end-diastolic diameter (LVEDD), lipid profiles (total cholesterol [TC], triglyceride [TG], low-density lipoprotein cholesterol [LDL-C], high-density lipoprotein cholesterol [HDL-C]), and inflammatory markers (C-reactive protein [CRP], procalcitonin [PCT]) were compared between the two groups before and after treatment. Major adverse cardiovascular events [MACEs] within 3 months after PCI and medication safety were also evaluated.</p><p><strong>Results: </strong>After treatment, cardiac output, LVEF, and HDL-C levels were significantly increased, while LVEDD, CRP, PCT, TC, TG, and LDL-C levels were significantly decreased in both groups, with significantly greater improvements observed in group A compared to group B (all P<0.05). The incidence of MACEs was significantly lower in group A than in group B (12.7% vs. 28.1%, P=0.027). No serious adverse drug reactions occurred in either group.</p><p><strong>Conclusion: </strong>Loading-dose atorvastatin can improve cardiac function, regulate lipid metabolism, suppress inflammatory responses, and reduce the incidence of short-term MACE in elderly AMI patients after PCI, while demonstrating good safety and clinical application value.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6759-6770"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploratory evaluation of the systemic inflammatory response index and internally evaluated machine learning models for prognosis in acute pesticide poisoning.","authors":"Qian Yu, Junsa Zhu, Feng Yang","doi":"10.62347/QLAP2073","DOIUrl":"10.62347/QLAP2073","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Purpose: &lt;/strong&gt;Acute pesticide poisoning (APP) is a life-threatening emergency with substantial mortality. This study aimed to evaluate whether the systemic inflammatory response index (SIRI) is associated with short-term prognosis in APP and to explore whether SIRI-containing machine learning models can support preliminary risk stratification. Because only 17 deaths occurred in this small single-center retrospective cohort and no external validation cohort was available, the analysis was designed and interpreted as exploratory rather than as development of a clinically deployable prediction model.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 127 patients with APP admitted to our hospital between October 2018 and December 2024 were retrospectively enrolled, including 110 survivors and 17 non-survivors. SIRI was calculated as absolute neutrophil count × absolute monocyte count/absolute lymphocyte count. All hypothesis tests were two-sided. Missing laboratory data were handled by complete-case screening for key variables and median imputation for variables with less than 10% missingness in model development. Logistic regression, random forest (RF), and support vector machine (SVM) analyses were performed only as exploratory internal modelling analyses using a predefined 70:30 training-internal hold-out split and repeated 10-fold cross-validation within the training set. Because the hold-out set was small and contained only a few deaths, ROC-derived cut-offs, threshold-dependent sensitivity/specificity, calibration metrics, and decision-curve results were not used as clinical validation evidence.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;SIRI was lower in the non-survival group than in the survival group (2.4 ± 3.1 vs. 6.1 ± 5.8; two-sided P = 0.042), consistent with the trend shown in Figure 3A. In multivariable logistic regression adjusted for RBC, D-dimer, APTT, AST, creatinine, glucose, albumin, and pesticide type, lower SIRI remained associated with mortality (adjusted OR = 0.86, 95% CI: 0.74-0.99, P = 0.041). However, given the low number of fatal events, the regression estimates, variable selection results, and machine learning performance metrics should be interpreted as statistically unstable candidate signals rather than externally generalizable evidence.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;Low SIRI was associated with mortality in this single-center APP cohort after adjustment for selected confounders, and exploratory models incorporating SIRI suggested that routine hematological and inflammatory variables may contain preliminary prognostic information. Nevertheless, because the death group included only 17 patients, mechanistic immune-inflammatory assays were not available, and no independent external validation cohort was included, SIRI should be regarded as a candidate blood-count-derived prognostic signal rather than a mechanistically proven biomarker. The RBC-SIRI pattern should not be used for clinical decision-making before prospe","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"7215-7225"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tripterygium glycosides and mesenchymal stem cells combination alleviated Sjögren's syndrome Features in mice and modulated the GRO-α/CXCR2 axis.","authors":"Jing Zhang, Xiaoqian Li, Yike Wang, Jing Hao, Yan Zhou","doi":"10.62347/HFYO1240","DOIUrl":"10.62347/HFYO1240","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the efficacy and potential mechanisms of tripterygium glycosides (TG) and human umbilical cord mesenchymal stem cells (hUC-MSCs) in the treatment of primary Sjögren's syndrome (PSS).</p><p><strong>Methods: </strong>Experimental Sjögren's syndrome (ESS) mice were administered TG (20 or 40 mg/kg/d, via oral gavage) and/or hUC-MSCs (1 × 10^6 cells/mouse, via tail vein injection) for a duration of 21 consecutive days. The parameters analyzed included saliva volume, lymphocytic infiltration in submandibular glands (SGs), serum cytokines, immunoglobulins, and the expression of the growth-related oncogene-alpha (GRO-α)/CXC chemokine receptor 2 (CXCR2) axis, as well as aquaporins (AQP1, AQP5).</p><p><strong>Results: </strong>Co-treatment with TG and hUC-MSCs resulted in a significant increase in saliva volume and a reduction in lymphocytic infiltration within the SGs. Additionally, this treatment decreased levels of pro-inflammatory cytokines (interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α) while enhancing the levels of anti-inflammatory cytokines (IL-10, IL-13) in serum, and modulated T helper (Th) 1 and Th2 cell populations. Furthermore, the combination therapy effectively suppressed elevated levels of immunoglobulins (immunoglobulin (Ig) M, IgG, IgA) in ESS mice. Mechanistically, the co-treatment inhibited the GRO-α/CXCR2 axis and upregulated the expression of AQP1 and AQP5 in SGs.</p><p><strong>Conclusion: </strong>The findings of this study suggest that co-treatment with TG and hUC-MSCs mitigates the symptoms of PSS by modulating inflammatory and immune responses and by inhibiting the GRO-α/CXCR2 axis.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6568-6579"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term impact of robot-assisted training on walking pattern reconstruction in chronic stroke survivors: insights from multiparametric gait analysis.","authors":"Zujie Qin, Qinhua Cao, Jie Wang, Peiyu Jiang, Xueping Sha","doi":"10.62347/YHML7031","DOIUrl":"10.62347/YHML7031","url":null,"abstract":"<p><strong>Aims: </strong>To determine whether robot-assisted lower-limb training promotes superior walking pattern reconstruction in chronic stroke survivors, as quantified by multiparametric gait analysis and supported by concurrent improvements in motor, balance, and functional outcomes.</p><p><strong>Methods: </strong>In this retrospective observational study, 80 patients with chronic stroke sequelae treated between January 2024 and January 2026 were allocated according to routine rehabilitation exposure to a control group (n = 45) or an observation group (n = 35); the primary endpoint was post-intervention gait remodeling, defined by change in gait speed and multiparametric spatiotemporal gait indices, while secondary endpoints included lower-limb motor recovery, spasticity, balance, functional independence, clinically meaningful responder status, and multivariable analysis of determinants of gait-speed change as the principal marker of walking pattern reconstruction.</p><p><strong>Results: </strong>After 10 weeks, the observation group showed significantly greater improvement than the control group in lower-limb motor function, including Fugl Meyer Assessment of the Lower Extremity total, proximal, and distal subscores, Timed Up and Go performance, and 10-m walking speed (all P < 0.001). Spasticity was also more effectively reduced, with lower Modified Ashworth Scale composite and segment-specific scores (P = 0.002 to < 0.001). In addition, balance, spatiotemporal gait parameters, and motor-related functional independence improved more markedly in the observation group (all P < 0.001 for key outcomes).</p><p><strong>Conclusion: </strong>Robot-assisted lower-limb rehabilitation, when added to conventional therapy, significantly enhanced gait remodeling, motor recovery, spasticity reduction, balance performance, and locomotion-related independence in chronic stroke survivors, supporting its value as an effective strategy for promoting more physiological and clinically meaningful walking restoration after stroke.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"7201-7214"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multi-omics atlas reveals PTM-driven signaling and immunologic remodeling in a mouse model of age-related subclinical hypothyroidism.","authors":"Kun Xu, Qing Zhou","doi":"10.62347/PQYE9899","DOIUrl":"10.62347/PQYE9899","url":null,"abstract":"<p><strong>Objective: </strong>Age-related subclinical hypothyroidism (AR-SCH) profoundly impairs thyroid structural and functional integrity in the elderly, yet the systemic molecular architecture driving AR-SCH pathogenesis remains poorly understood. This study aimed to construct a comprehensive multi-omic atlas of the AR-SCH mouse thyroid.</p><p><strong>Methods: </strong>Global proteomics, site-specific N-glycoproteomics, and phosphoproteomics were integrated to profile thyroid tissues from AR-SCH and control mice.</p><p><strong>Results: </strong>In total, 6,666 proteins, 2,476 site-specific N-glycans, and 11,929 phosphorylation sites were identified. Global proteomics revealed a widespread reduction of functional proteins (81.7% downregulated), primarily associated with cytoskeletal structural remodeling and tissue degeneration under AR-SCH conditions. N-glycoproteomics identified altered fucosylation and reduced N-glycosylation associated with extracellular-matrix organization and local immune-related processes. Furthermore, an extensive hypophosphorylation trend (91.53% downregulated sites) was associated with suppression of intracellular signaling cascades, possibly involving autophagic clearance and perturbed apoptotic homeostasis.</p><p><strong>Conclusion: </strong>Multi-omics integration suggests an interconnected network as a convergent hallmark of AR-SCH progression, characterized by cytoskeletal reconfiguration, immune regulation shifts, and signaling attenuation that synergistically contribute to systemic thyroid decline, offering a therapeutic window for preserving endocrine homeostasis.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"7075-7091"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influencing factors of general anesthesia and spinal anesthesia for cesarean section women with PAH.","authors":"Jinlian Ai, Rong Liu, Hanliang Jiang, Nanyan Xi","doi":"10.62347/XECE9153","DOIUrl":"10.62347/XECE9153","url":null,"abstract":"<p><strong>Objective: </strong>To compare the clinical efficacy and safety of general anesthesia versus spinal anesthesia for cesarean section in parturients with pulmonary arterial hypertension (PAH), and analyze the influencing factors for postoperative adverse outcomes.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted on 236 PAH parturients who underwent cesarean section from January 2022 to December 2024. All subjects were grouped via propensity score matching into the general anesthesia group (Group A, n=104) and the spinal anesthesia group (Group B, n=132). Intraoperative hemodynamic parameters, surgical indicators, postoperative adverse events, and neonatal outcomes were compared between the two groups. Subgroup analysis, univariate and multivariate Logistic regression analyses, receiver operating characteristic (ROC) curve analysis, and forest plot analysis were performed for statistical evaluation.</p><p><strong>Results: </strong>At time points T1 to T3, Group B presented significantly lower mean arterial pressure and heart rate with milder hemodynamic fluctuations, less intraoperative blood loss, and a lower overall incidence of adverse events than Group A (all P<0.05). In subgroups of severe PAH and New York Heart Association (NYHA) class III-IV cardiac function, Group B also showed a reduced adverse event rate (P<0.05). Multivariate Logistic regression analysis identified preoperative NYHA classification, anesthesia modality, PAH severity, and preoperative hemoglobin level as independent influencing factors for postoperative adverse outcomes (all P<0.05). ROC curve analysis demonstrated that the combined detection of the above three core indicators yielded superior predictive efficacy compared with single-index detection (P<0.05). The ROC curve of the validation group had an area under the curve (AUC) of 0.739, indicating favorable predictive performance of the model. The calibration curve verified excellent calibration capability, with predicted values highly consistent with actual clinical outcomes. The decision curve analysis showed that the nomogram model maintained positive net clinical benefits within a probability threshold range of 10-90%.</p><p><strong>Conclusion: </strong>Spinal anesthesia provides safer and more stable perioperative hemodynamic conditions for PAH parturients undergoing cesarean section. The combined evaluation of preoperative clinical indicators can effectively predict postoperative adverse outcomes in this patient population.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6807-6822"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of serum TIM-3 and Galectin-9 levels with glaucoma progression: implications for clinical monitoring.","authors":"Zihui Liu, Zong Wang, Linpin Liu, Bin Tu, Kai Wang","doi":"10.62347/HUVJ6501","DOIUrl":"10.62347/HUVJ6501","url":null,"abstract":"<p><strong>Background: </strong>Primary open-angle glaucoma (POAG) causes irreversible blindness via retinal ganglion cell loss. Neuroinflammation and immune dysfunction drive its progression, yet the role of the TIM-3/Galectin-9 axis remains unclear.</p><p><strong>Objective: </strong>To investigate the association of serum soluble TIM-3 (sTIM-3) and Galectin-9 levels with the progression of primary open-angle glaucoma (POAG).</p><p><strong>Methods: </strong>This retrospective analysis enrolled POAG patients from our ophthalmology department (January 2022-December 2024). After a minimum 12-month follow-up, participants were categorized as progression or stable based on visual field and optical coherence tomography (OCT) assessments. Healthy controls were age/sex-matched. Recorded clinical variables included IOP, CCT, visual fields, and OCT measures. Baseline concentrations of sTIM-3 and Galectin-9 in serum were determined by ELISA. Multivariate logistic regression assessed independent links between these markers and disease progression, supplemented by trend tests, E-value calculations, and subgroup analyses.</p><p><strong>Results: </strong>The study included 120 POAG patients (52 progression, 68 stable) and 60 healthy controls. Baseline measures were balanced across groups. Both sTIM-3 and Galectin-9 were significantly elevated in progression relative to stable patients and controls (all P < 0.001). Multivariate logistic regression identified sTIM-3 (OR = 2.14, P = 0.004) and Galectin-9 (OR = 3.39, P < 0.001) as independent factors linked to disease progression. Trend analyses revealed dose-response patterns (P for trend = 0.015 and 0.004, respectively). These findings were further corroborated by E-value and subgroup analyses, supporting their robustness.</p><p><strong>Conclusions: </strong>Higher baseline sTIM-3 and Galectin-9 levels correlate independently with POAG progression risk, showing a dose-response pattern. These biomarkers may serve as adjunctive references for risk stratification, although their utility warrants prospective validation with longitudinal sampling.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"7306-7319"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic changes in blood-derived inflammatory and bone metabolic markers for disease monitoring and prognostic assessment in multiple myeloma.","authors":"Ruili Yuan, Jingjing Yin, Juan Liu, Ni Liu, Xuan Guo, Xiaoqin Wang","doi":"10.62347/JCWP2716","DOIUrl":"10.62347/JCWP2716","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical value of dynamic changes in inflammatory (NLR, PLR, LMR) and bone metabolic markers (β-CTX, PINP, BALP, serum calcium) before and after treatment for disease monitoring and prognosis in multiple myeloma (MM), and to validate these indicators in an independent cohort.</p><p><strong>Methods: </strong>A derivation cohort (2018-2021, n = 402) and a temporal validation cohort (2021-2023, n = 218) were retrospectively enrolled. Patients were stratified into remission (n = 260) and non-remission (n = 142) groups. Dynamic changes (Δ) in inflammatory and bone metabolic markers were compared between groups. ANC and ALC were analyzed to elucidate NLR dynamics. Univariate Cox regression, collinearity diagnostics, and time-varying Cox regression were used to identify prognostic variables. Sensitivity analyses and external validation were performed.</p><p><strong>Results: </strong>ΔPLR, ΔLMR, Δβ-CTX, ΔPINP, ΔBALP, and Δserum calcium differed significantly between groups (all P < 0.001), while ΔNLR did not (P = 0.616). Δβ-CTX showed the largest effect size. The derived neutrophil-to-lymphocyte ratio (dNLR) (HR 0.691, P < 0.001) and derived β-C-terminal telopeptide of type I collagen (dBCTX) (HR 4.205, P < 0.001) were independently associated with overall survival (OS). dNLR exhibited a time-dependent effect strengthening over follow-up, with pronounced prognostic value in R-ISS stage III patients (P < 0.001). Post-treatment ALC was also associated with OS (HR 0.767, P < 0.001). Results were confirmed in the validation cohort (dNLR: HR 0.595; dBCTX: HR 3.911; both P < 0.001).</p><p><strong>Conclusion: </strong>Dynamic changes in inflammatory and bone metabolic markers effectively reflect treatment response in MM. The dNLR and dBCTX were identified as independent prognostic factors of OS that represent immune function and bone repair. In a temporally independent cohort, the blood-derived markers were validated, supporting their clinical utility for disease monitoring and risk stratification.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6458-6482"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13589837/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}