American journal of translational research最新文献

{"title":"Association between nutritional risk and clinical outcomes in patients with acute exacerbation of chronic obstructive pulmonary disease.","authors":"Yang Yang, Manqing You, Lincheng Luo, Hailong Wei","doi":"10.62347/MCZH5861","DOIUrl":"10.62347/MCZH5861","url":null,"abstract":"<p><strong>Objective: </strong>To explore the relationship between nutritional risk and clinical outcomes in patients hospitalized for acute exacerbations of chronic obstructive pulmonary disease COPD (AECOPD).</p><p><strong>Methods: </strong>The medical records of 220 AECOPD patients hospitalized between June 2022 and June 2024 were retrospectively analyzed. The patients were categorized into two groups based on their Nutritional Risk Index (NRI): high-risk ([NRI] < 92) group and low-risk (NRI ≥ 92) group. Clinical outcomes assessed included albumin levels, arterial blood gas parameters, frequency of exacerbations, in-hospital mortality, length of hospital stays, readmission rates, and health-related quality of life (HRQoL). Pulmonary function recovery, including forced expiratory volume in one second (FEV₁) and forced vital capacity (FVC), was also evaluated post-treatment.</p><p><strong>Results: </strong>Compared to the low-risk group, patients in the high-risk group exhibited significantly lower albumin levels (P = 0.007), increased frequency of exacerbations (P = 0.005), higher in-hospital mortality (P = 0.004), prolonged hospital stays (P = 0.001), and elevated readmission rates (P = 0.002). High-risk patients also reported significantly lower physical function (PF) and mental health scores. After treatment, improvements in FEV₁ and FVC were significantly greater in the low-risk group (P < 0.05).</p><p><strong>Conclusion: </strong>Nutritional risk is closely associated with the severity, prognosis, and recurrence of AECOPD. These findings underscore the importance of nutritional assessment and intervention in the management of hospitalized AECOPD patients.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 7","pages":"5746-5756"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Case report: surgery combined with a SOX adjuvant chemotherapy regimen for patients with gastric adenocarcinoma and hepatocellular carcinoma.","authors":"Zhe-Yuan Wang, Lu Zhang, Zeng-You Li, You-Cheng Zhang, Jing-Jing He","doi":"10.62347/FFUV4293","DOIUrl":"10.62347/FFUV4293","url":null,"abstract":"<p><p>Advances in early tumor detection have led to an increase in the reported incidence of multiple primary cancers. However, the concurrent occurrence of gastric adenocarcinoma and hepatocellular carcinoma remains rare. We present the case of a 68-year-old male patient with chronic hepatitis B who was diagnosed with stage Ib liver cancer (T1bN0M0). Gastroscopy revealed a mucosal lesion in the posterior wall of the gastric body, and biopsy confirmed moderately to poorly differentiated adenocarcinoma. The patient underwent laparoscopic gastrectomy combined with partial liver resection, followed by three cycles of S-1 plus oxaliplatin (SOX) chemotherapy. Given the lack of standardized treatment protocols for managing multiple primary cancers, developing a treatment plan for these two synchronous cancers is challenging. This case underscores the importance of early screening for multiple primary cancers and the need for a multidisciplinary approach to formulate an optimal treatment strategy. Furthermore, we conducted a literature review of reports on the simultaneous occurrence of gastric and hepatic cancers over recent decades. Unlike previous studies, we also examined postoperative adjuvant chemotherapy regimens suitable for both gastric adenocarcinoma and hepatocellular carcinoma patients.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 7","pages":"5710-5717"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DCUN1D2 is insignificant for spermatogenesis and male fertility in mice.","authors":"Yuxuan Feng, Mingyuan Bao, Wenyi Sheng, Shiqi Liu, Yuchen Cui, Nianchao Zhou, Tiantian Wu, Xiaoyan Huang, Shunyu Hou, Meng Liu","doi":"10.62347/CLIG5523","DOIUrl":"10.62347/CLIG5523","url":null,"abstract":"<p><strong>Objectives: </strong>Defective in cullin neddylation 1 domain containing 2 (DCUN1D2) belongs to the DCNLs family and can induce cell growth arrest and is also related to neddylation. While its potential role in spermatogenesis is hypothesized, the functional significance of DCUN1D2 in male germ cells remains undefined.</p><p><strong>Methods: </strong>To investigate this, germ cell and Sertoli cell-specific <i>Dcun1d2</i>-knockouts were generated. Sperm parameters were analyzed via computer-assisted sperm analysis (CASA), while histological and immunofluorescence staining were employed to evaluate spermatogenic progression and apoptosis.</p><p><strong>Results: </strong>Compared with the control group, <i>Dcun1d2</i> conditional knockout (cKO) mice exhibited no significant differences in histology, semen quality, sperm apoptosis or fertility tests.</p><p><strong>Conclusions: </strong>This study indicated that DCUN1D2 has no significant effect on mouse spermatogenesis or male fertility. These findings will help avoid redundant studies and provide new information for the study of human fertility genes.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 7","pages":"5614-5624"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of risk factors and development of a high-performance predictive model for non-healing in elderly patients with intertrochanteric fractures post-internal fixation.","authors":"Jianyue Wu, Peng Xu, Dong Zhang, Yingjie Ni, Jijun Zhao","doi":"10.62347/KFRM4177","DOIUrl":"10.62347/KFRM4177","url":null,"abstract":"<p><strong>Objective: </strong>To identify risk factors associated with non-healing in elderly patients with intertrochanteric femoral fractures treated with internal fixation and to develop a predictive model for non-union risk.</p><p><strong>Methods: </strong>We conducted a retrospective analysis of 889 elderly patients treated with internal fixation for intertrochanteric fractures at Wuxi Xishan People's Hospital from March 2021 to December 2024. Patients were classified into healing (n=806) and poor healing groups (n=83) based on radiographic evidence three months post-surgery. Univariate and multivariate logistic regression analyses were used to identify significant risk factors. A predictive model was developed and validated using receiver operating characteristic (ROC) analysis and the area under the curve (AUC).</p><p><strong>Results: </strong>Significant risk factors for poor healing included smoking history (Odds ratio [OR] 1.750, P=0.022), osteoporosis (OR 2.055, P=0.003), posterior or medial wall bone defects (OR 1.964, P=0.005), low postoperative albumin (OR 1.674, P=0.032), and early weight-bearing (OR 1.765, P=0.018). The use of proximal femoral nail antirotation (PFNA) significantly reduced the risk of poor-healing (OR 0.515, P=0.006). The combined predictive model achieved an AUC of 0.949, indicating high predictive value.</p><p><strong>Conclusions: </strong>Our findings highlight key risk factors for non-healing in elderly patients post-internal fixation for intertrochanteric fractures. The developed predictive model, incorporating clinical, biochemical, and surgical factors, offers high accuracy and may help identify high-risk patients for targeted intervention.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 7","pages":"5766-5778"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA HCP5 promotes the progression of gastric cancer through the miR-526b/PBX3 axis.","authors":"Guang-Chuan Mu, Xue-Yu Zeng, Chao-Zhen Hu","doi":"10.62347/IOYF4383","DOIUrl":"10.62347/IOYF4383","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate whether LncRNA HLA complex P5 (HCP5) promotes gastric cancer (GC) via the miR-526b/Pre-B Cell Leukemia Homeobox 3 (PBX3) pathway.</p><p><strong>Methods: </strong>Thirteen paired GC and adjacent non-tumorous tissues, along with NCI-N87 GC cells, were analyzed. HCP5 expression levels were measured, and its impact on cell viability, proliferation, and migration were evaluated. Dual-luciferase reporter assays were performed to confirm the direct interactions among HCP5, miR-526b, and PBX3. The effects of HCP5 overexpression or silencing on miR-526b and PBX3 expression were analyzed. A miR-526b mimic was transfected for functional rescue.</p><p><strong>Results: </strong>HCP5 was significantly upregulated, while miR-526b was downregulated in GC tissues. Dual-luciferase assays confirmed the direct binding of HCP5 to miR-526b and of miR-526b to PBX3. In NCI-N87 cells, HCP5 overexpression downregulated miR-526b and upregulated PBX3 expression, whereas silencing HCP5 showed the opposite effects. Moreover, HCP5 overexpression decreased Bax and increased Bcl-2 levels, which was reversed by miR-526b mimic transfection. Functionally, HCP5 enhanced GC cell viability and migration, both of which were suppressed by miR-526b. HCP5 promoted cell proliferation, as evidenced by a reduced proportion of cells in the G0/G1 phase, which was reversed by miR-526b.</p><p><strong>Conclusions: </strong>HCP5 acts as an oncogenic lncRNA in GC by promoting cell viability, migration, and proliferation via the miR-526b/PBX3 axis. Targeting the HCP5/miR-526b/PBX3 axis may represent a promising therapeutic strategy for GC.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 7","pages":"5602-5613"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanical thrombectomy combined with tirofiban for treatment of acute intracranial atherosclerotic cerebral infarction: clinical observations and effect on serum inflammatory factors.","authors":"Ben-Yang Chen, Yu-Zhang Bei, Jian-Huang Chen, Wei-Li Bai","doi":"10.62347/FJGC1286","DOIUrl":"10.62347/FJGC1286","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the clinical efficacy of mechanical thrombectomy (MT) combined with tirofiban in treating acute intracranial large artery atheromatous cerebral infarction and its effect on inflammatory factor levels.</p><p><strong>Methods: </strong>This retrospective study included 102 patients with acute intracranial large atherosclerotic cerebral infarction admitted to Jili Hospital, Liuyang City, Hunan Province, between December 2022 and December 2024. Patients were divided into a control group (MT) and an observation group (MT + tirofiban). Pre-treatment and post-treatment assessments included revascularization rate, hypersensitive C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), D-dimer (D-D), prothrombin time (PT), activated partial thromboplastin time (APTT), neuron-specific enolase (NSE), central neural specific protein (S100β), and myelin basic protein (MBP). Neurological function was evaluated using the National Institutes of Health Stroke Scale (NIHSS), Mini-Mental State Examination (MMSE), modified Barthel index (MBI), and Fugl-Meyer assessment (FMA). Logistic analysis was used to identify factors influencing vascular re-canalization.</p><p><strong>Results: </strong>After treatment, the revascularization rate was higher in the observation group (94.12% vs. 78.43%, P<0.05). NIHSS, D-D, hs-CRP, TNF-α, IL-6, NSE, S100β, and MBP levels decreased significantly, with lower values observed in the observation group (P<0.05). MMSE, MBI, and FMA scores increased more in the observation group (P<0.05). PT and APTT were prolonged significantly, with higher values in the observation group (P<0.05). Logistic regression identified post-treatment NIHSS score as an independent risk factor for re-occlusion (P<0.05).</p><p><strong>Conclusion: </strong>Tirofiban combined with MT improves revascularization in patients, reduces inflammation and neurobiochemical damage, enhances neurological function, and lowers the risk of re-occlusion in patients with acute intracranial large artery atheromatous cerebral infarction.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 7","pages":"5689-5697"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofeedback magnetic stimulation improves postoperative bowel dysfunction after rectal cancer surgery.","authors":"Yang Lin, Gang Mao, Jinghua Liang, Lu Liu","doi":"10.62347/XBDR6986","DOIUrl":"10.62347/XBDR6986","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the clinical efficacy of biofeedback magnetic stimulation (BFMS) in the management of postoperative bowel dysfunction (PBD) after rectal cancer surgery and to identify independent predictors of treatment outcomes.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted involving 158 patients who underwent low anterior resection between 2018 and 2023. Patients were divided into two groups: the observation group, consisting of 83 individuals, received BFMS; whereas the control group, comprising 75 individuals, underwent conventional rehabilitation. Defecation function, anorectal manometric parameters - including resting and pressure (RAP), maximum squeeze pressure (MSP), and maximum tolerated volume (MTV) - as well as Wexner scores and QLQ-CR29 quality-of-life scores were assessed at 1, 2, and 3 months postoperatively.</p><p><strong>Results: </strong>At 2 and 3 months postoperatively, the observation group showed significantly greater improvement in defecation function, anorectal pressure metrics, and quality of life scores compared to controls (P<0.05). The incidence of adverse reactions was comparable between groups (14.46% vs. 6.67%, P>0.05). Multivariate analysis revealed that anastomotic leakage, ultra-low rectal cancer, interspinous diameter, and treatment method were independent predictors of postoperative outcomes (P<0.05).</p><p><strong>Conclusion: </strong>BFMS is a safe and effective therapeutic approach for improving bowel function and quality of life in patients with PBD after rectal cancer surgery. Anatomical and surgical variables may serve as important predictors of treatment response.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 7","pages":"5667-5678"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351603/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical analysis of hospital-acquired infections in patients with multiple myeloma: a study of 200 cases.","authors":"Hece Cai, Fugui Sun, Weihua Shen, Xianghai Bian","doi":"10.62347/ZGYB9556","DOIUrl":"10.62347/ZGYB9556","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical characteristics of hospital-acquired infections (HAIs) in patients undergoing treatment for multiple myeloma.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 200 patients with multiple myeloma treated at Haiyan County People's Hospital in Jiaxing. The incidence rate, sites of infection, and causative pathogens of HAIs were recorded. Patients were divided into an infection group (n=37) and a non-infection group (n=163) based on the presence of HAIs. According to follow-up outcomes, they were further classified into a recurrence group (n=76) and a non-recurrence group (n=124). Risk factors associated with HAIs in patients with multiple myeloma were analyzed, and the predictive value of serum immunoglobulin A (IgA) and immunoglobulin G (IgG) levels for the recurrence of multiple myeloma was assessed.</p><p><strong>Results: </strong>Among the 200 patients with multiple myeloma, the incidence of HAIs was 18.5%. The respiratory tract was the most commonly affected site, with Gram-negative bacilli being the predominant pathogens. Multivariate logistic regression analysis identified neutropenia, age ≥60 years, chemotherapy, low serum albumin levels, high Revised International Staging System (R-ISS) stage, elevated body mass index (BMI), and comorbid diabetes mellitus as significant risk factors for infection following multiple myeloma treatment (all P<0.05). In addition, receiver operating characteristic (ROC) analysis revealed that serum IgA and IgG levels were strong predictors for recurrence in patients with multiple myeloma, with area under the curve (AUC) values of 0.936 and 0.914, respectively. IgA showed a sensitivity of 97.8% and specificity of 93.5%, while IgG demonstrated a sensitivity of 83.3% and specificity of 80.0%.</p><p><strong>Conclusion: </strong>Patients with multiple myeloma are at increased risk of developing HAIs after treatment, particularly respiratory infections caused by Gram-negative bacilli. Moreover, serum IgA and IgG levels may serve as reliable biomarkers for predicting disease recurrence.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 7","pages":"5757-5765"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"YAG laser combined with mechanical debridement enhances pain relief and inflammation control in the management of periodontitis with peri-implantitis.","authors":"Dawei Sun, Zhiqiang Li, Daxu Li, Baolei Wu, Yiwei Ma","doi":"10.62347/VIOT6629","DOIUrl":"10.62347/VIOT6629","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the efficacy of Er: YAG laser combined with mechanical debridement in treating periodontitis with peri-implantitis.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 292 patients treated between 2018 and 2024. The patients were divided into an observation group (n=139, Er: YAG laser + mechanical debridement) and a control group (n=153, mechanical debridement only). Outcome measures included Visual Analog Scale (VAS) scores, periodontal probing depth (PD), clinical attachment level (CAL), bleeding index (BI), gingival index (GI), and TNF-α/IL-6 levels. Clinical efficacy and risk factors for treatment failure were analyzed using logistic regression.</p><p><strong>Results: </strong>The observation group exhibited a significantly higher total efficacy rate than the control group (89.21% vs. 64.71%, P<0.001). Additionally, VAS scores, PD, CAL, BI, GI, and TNF-α/IL-6 levels were significantly lower at 1 week, 2 weeks, and 1 month post-treatment in the observation group (all P<0.05). Multivariate logistic regression identified age (OR=1.12, 95% CI: 1.07-1.18, P<0.001), smoking (OR=6.21, 95% CI: 2.96-13.82, P<0.001), and diabetes (OR=5.74, 95% CI: 2.71-12.94, P<0.001) as independent risk factors for treatment failure, while postprandial gargling (OR=0.28, P=0.002) was identified as a protective factor.</p><p><strong>Conclusion: </strong>Er: YAG laser combined with mechanical debridement significantly reduced pain and inflammation, improving peri-implant health. Smoking, diabetes, and age increased treatment failure risk, while postprandial gargling was protective, underscoring the importance of personalized treatment strategies.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 7","pages":"5698-5709"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The importance of marginal sclerosis in the recovery of mechanical strength after curettage of bone lesions.","authors":"Francisco de As Baima Filho, Priscylla G Mendonça, Giuliano F Morgantetti, Alice C Silva-Sousa, Leonardo R Battaglion, Ramon Syrc Silva, Maria do S de S Cartagenes, Edgard E Engel","doi":"10.62347/GZLN4148","DOIUrl":"10.62347/GZLN4148","url":null,"abstract":"<p><strong>Objectives: </strong>This study investigated the role of marginal sclerosis in the recovery of mechanical strength following curettage and grafting of osseous lesions. To date, it remains unclear as to whether marginal sclerosis fully or partially restores the mechanical resistance of the affected bone to compressive, flexural, and torsional loads.</p><p><strong>Methodology: </strong>Adult male <i>Rattus norvegicus</i> rats (WISTAR albino strain) aged approximately 90-days were used. Bone defects were created in the tibiae, and the animals were subsequently euthanized at different time points for outcome analysis. Various assessment methods were employed, including radiography, histology, immunohistochemistry, microcomputed tomography (micro-CT), mechanical bending and torsion tests.</p><p><strong>Results: </strong>This study revealed a progressive thickening of the osseous window borders before complete closure and a significant correlation between the intensity of marginal sclerosis that was radiographically observed, the rate of bone formation on histology, and the newly formed bone volume on micro-CT. Radiographs revealed thin marginal sclerosis at seven days post surgery, with a significant increase in sclerosis thickness being observed at ten days, whereas micro-CT revealed proportions of new bone formation of 44.80% at seven days and 105.70% at ten days. Additionally, at seven days, the mechanical resistance of the operated bones to torsion and bending was comparable to that of the non-operated bones, thereby indicating a complete recovery of mechanical strength.</p><p><strong>Conclusion: </strong>This study revealed that the appearance of marginal sclerosis around bone defects (as identified by radiography) is associated with the complete recovery of mechanical strength in rat tibiae under both bending and torsion.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 7","pages":"5641-5653"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144870999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}