American journal of translational research最新文献

{"title":"Exploring the landscape of autoimmune disorder-associated genes and their impact on immune microenvironment in breast cancer patients.","authors":"Weiwei Peng, Rong Wang, Owais Qadeer Gill, Mumtaz Hussain, Abdulaziz Alamri, Saeedah Almutairi, Salman Alrokayan, Rida Naz, Ashnah Iqbal, Tahani Alnumasi, Mostafa A Abdel-Maksoud","doi":"10.62347/EANH4082","DOIUrl":"10.62347/EANH4082","url":null,"abstract":"<p><strong>Objectives: </strong>Breast cancer (BC) remains a leading cause of cancer-related mortality among women worldwide. Emerging evidence suggests that autoimmune-associated genes may critically influence tumor progression and immune evasion. This study investigates the dysregulation of autoimmune-associated genes in BC and explores their potential roles in immune modulation and tumor progression.</p><p><strong>Methods: </strong>Two publicly available bulk RNA-sequencing datasets (GSE233242 and GSE227679) were analyzed to identify differentially expressed genes (DEGs) between BC and normal tissues. A curated set of autoimmune-related genes was compiled from multiple databases, including ClinVar, UniProt, OMIM, MedlinePlus, GWAS Catalog, and GeneCards. Enrichment analysis was conducted using KEGG pathways via Enrichr, and protein-protein interaction (PPI) networks were constructed using STRING and Cytoscape to identify hub genes.</p><p><strong>Results: </strong>Out of 3,676 autoimmune genes, 125 were found to be upregulated and 75 downregulated in BC tissues. Key enriched pathways included Cytokine-cytokine receptor interaction, Chemokine signaling, IL-17 signaling, and PI3K-Akt signaling. Ten hub genes were identified, with CXCR4, MMP9, CTLA4, CD80, and ICOS upregulated, and IL6, KIT, PPARG, SLC2A4, and CEBPA downregulated. CXCR4 and MMP9 overexpression was associated with increased metastasis, while downregulation of KIT and PPARG indicated impaired immune surveillance and poorer prognosis.</p><p><strong>Conclusions: </strong>This study reveals the pivotal role of autoimmune gene dysregulation in BC progression and immune microenvironment remodeling. Integrating autoimmune gene signatures with transcriptomic data provides novel insights into BC pathogenesis and highlights potential biomarkers and therapeutic targets for immune-based interventions.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 6","pages":"4733-4743"},"PeriodicalIF":1.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered expression of TEF gene in childhood constipation leads to a decrease in CD56⁺ NK cells.","authors":"Yingying Wang, Ziyi Li, Lixiang Li, Houyan Huang, Heqing Cui, Lili Zhang, Li'e Xiang, Sujuan Liu, Ye Jiang, Ping Xu, Taocheng Zhou, Hui Peng, Peisen Wang, Lili Shang","doi":"10.62347/WZAO9129","DOIUrl":"10.62347/WZAO9129","url":null,"abstract":"<p><strong>Objectives: </strong>This study used a quantitative bioinformatic analysis of public RNA sequencing databases to study key molecules mediating the occurrence of childhood constipation (CHC) and explore associated immune cell abnormalities and the role of natural killer (NK) cells.</p><p><strong>Methods: </strong>Gene expression profiling datasets, including CHC (GSE36701), were obtained. Immune genes were downloaded from the MSigDB database, and 3,907 immune-related genes were obtained. Differential analysis and weighted gene co-expression network analysis identified 12 hub genes related to CHC and immune genes in adipose tissue and blood samples from GSE36701. Gene ontology analysis was performed to determine key biologic processes. Stepwise and logistic regression analyses were performed to select specific genes for constructing a diagnostic model for CHC. The model was validated using GSE36701, and its diagnostic performance was assessed using the AUC value. The study recruited 20 CHC patients and 20 HV children. After blood collection, peripheral blood mononuclear cells (PBMCs) were extracted, and flow cytometry was used to detect the proportions of immune cells in the blood. qRT-PCR was employed to measure the expression of hub genes in NK cells.</p><p><strong>Results: </strong>A total of 12 hub genes were identified, among which the regulation of steroid metabolic processes and renal sodium excretion were closely associated with an increased expression level of CHC. Gene set enrichment analysis revealed that the core genes were associated with glycosphingolipid biosynthesis ganglio series, base excision repair, and ribosome characteristics. Immunoinfiltration analysis and experimental findings showed that the proportion of CD56⁺ NK cells in patients with CHC was significantly lower compared to healthy children. The qRT-PCR results indicated that compared to the healthy volunteers group, the expression of AGTR1, FAM200B, NRSN2-AS1, PRAC1, SERTAD3, and TEF genes was decreased, while the expression of APANXA2-OT1, FMO9P, and LOC100506929 genes was increased in the CHC group.</p><p><strong>Conclusion: </strong>This study identified TEF as a hub gene associated with the coexistence of CHC and immune cell abnormalities. The study highlights the important role of CD56⁺ NK cells in the pathogenesis of CHC and provides possible targets for diagnosis and therapeutic intervention.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 6","pages":"4135-4147"},"PeriodicalIF":1.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benefit of nanohydroxyapatite combined with triamcinolone for non-surgical treatment of severe periodontitis: a retrospective study.","authors":"Long Chen, Fujun Chen, Fengming Xue, Lianghui Yang","doi":"10.62347/WGSE9643","DOIUrl":"10.62347/WGSE9643","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the possible benefit of combining nanohydroxyapatite (nHA) with triamcinolone (TR) in the non-surgical management of severe periodontitis and to assess the influence of baseline inflammation on treatment outcomes.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 120 patients who received one of the following local treatments: nHA+TR, nHA alone, TR alone, or conventional subgingival scaling. All patients were followed for 6 months. Clinical data - including probing depth (PD), clinical attachment level (CAL), periodontal pocket closure rate, bleeding on probing (BoP), plaque index (PI), gingival index (GI), and gingival recession (GR) - along with inflammatory biomarkers [interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and C-reactive protein (CRP)] were evaluated over the follow-up period. Correlation, regression, and subgroup analyses were performed.</p><p><strong>Results: </strong>The nHA+TR group showed the greatest improvements in PD, CAL, and pocket closure rate, along with significant reductions in IL-1β, TNF-α, IL-6, and CRP (all P < 0.01). Changes in PD and CAL were strongly correlated with declines in inflammatory markers (r > 0.80, both P < 0.001). Multivariable analysis identified combination therapy and higher baseline PD, PI, and BoP as positive predictors of clinical improvement, whereas elevated baseline CRP and IL-1β were associated with poorer outcomes (all P < 0.01). Treatment with nHA+TR was significantly associated with clinical success - defined as a ≥ 2 mm reduction in PD and ≥ 1 mm gain in CAL (odds ratio = 48.49; all P < 0.001). In patients with CRP > 3 mg/L, combination therapy showed enhanced clinical benefits, with a significant interaction between treatment and baseline inflammation (P_interaction = 0.032). All interventions were well tolerated, with no serious adverse events reported.</p><p><strong>Conclusions: </strong>The combination of nHA and TR may enhance both clinical and inflammatory outcomes in the non-surgical treatment of severe periodontitis. Further randomized controlled trials are warranted to confirm these findings.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 6","pages":"4663-4678"},"PeriodicalIF":1.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of inflammatory protein expression patterns and their association with viral DNA load in hepatitis B virus infection via Olink proteomics analysis.","authors":"Xiangjuan Li, Lingming Lu, Rong Gao, Jie Gan","doi":"10.62347/JTXH6594","DOIUrl":"10.62347/JTXH6594","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the differential expression of inflammatory proteins in the sera of patients with chronic hepatitis B (CHB) using Olink Targeted Proteomics and to explore their correlations with viral deoxyribonucleic acid (DNA) load.</p><p><strong>Methods: </strong>This retrospective study included 66 CHB patients and 22 healthy controls, with medical records collected between January and December 2023 at Nanning Customs, China. Viral DNA loads were quantified using real-time polymerase chain reaction (PCR), and 92 inflammatory proteins were profiled using Olink Targeted Proteomics.</p><p><strong>Results: </strong>A total of 38 proteins were differentially expressed between CHB patients and healthy controls, of which 7 were upregulated and 31 were downregulated. Correlation analysis revealed that viral DNA load was positively associated with the expression of osteoprotegerin (OPG) (P = 0.021) and chemokine (C-X-C motif) ligand 9 (CXCL9) (P = 0.002), and negatively associated with interleukin-10 (IL-10) (P = 0.007), cluster of differentiation 40 (CD40) (P = 0.004), and caspase-8 (CASP8) (P = 0.020). Functional enrichment analysis indicated that these proteins were mainly enriched in neutrophil chemotaxis, granulocyte migration, chemokine signaling pathways, cytokine activity, chemokine activity receptors, and tumor necrosis factor (TNF) receptors.</p><p><strong>Conclusions: </strong>Specific inflammatory proteins, including OPG, CXCL9, IL-10, CD40, CASP8, are associated with viral DNA load in CHB patients. These findings enhance the proteomic understanding of HBV pathogenesis and may offer potential therapeutic targets and biomarkers.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 6","pages":"4701-4712"},"PeriodicalIF":1.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between the severity of lumbar spinal stenosis and lumbar paraspinal muscle atrophy.","authors":"Xiaoyu Mu, Chongyu Zhao, Zhitao Shan, Li Deng, Xiaowei Zhou, Li Li","doi":"10.62347/HKEC4010","DOIUrl":"10.62347/HKEC4010","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the correlation between the severity of lumbar spinal stenosis (LSS) and the atrophy of lumbar paraspinal muscles.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 200 patients with LSS (stenosis group) and 60 individuals without lumbar spine disease (control group) treated at the Department of Orthopedics, Kunming Traditional Chinese Medicine Hospital, from January 2022 to October 2024. Using a 1.5T MRI system, we measured the total cross-sectional area (TCSA) and total fat-free cross-sectional area (TFCSA) of the multifidus, erector spinae, and psoas major muscles. Muscle atrophy was evaluated using the TFCSA/TCSA ratio, and its correlation with LSS severity was analyzed.</p><p><strong>Results: </strong>The stenosis group showed significantly lower TFCSA/TCSA ratios in the multifidus, erector spinae, and psoas major compared to controls (P<0.05). LSS severity was negatively correlated with the TFCSA/TCSA ratios of the multifidus (r=-0.504, P<0.05) and erector spinae (r=-0.562, P<0.05), but not with the psoas major (P>0.05). Similarly, the number of stenotic segments was negatively correlated with multifidus (r=-0.381) and erector spinae (r=-0.420) atrophy (P<0.05). TFCSA/TCSA ratios were significantly lower on the symptomatic side for all three muscles (all P<0.05).</p><p><strong>Conclusion: </strong>The severity and extent of LSS are significantly associated with atrophy of the multifidus and erector spinae, but not the psoas major. Greater muscle atrophy corresponds to a higher number of stenotic segments.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 6","pages":"4247-4255"},"PeriodicalIF":1.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic efficacy of different sulperazon dosing regimens in carbapenem-resistant Klebsiella pneumoniae: a retrospective analysis of 209 cases.","authors":"Yao Chen, Qingjiang Guo, Youbao Liang, Erhu Heng","doi":"10.62347/TZFB9853","DOIUrl":"10.62347/TZFB9853","url":null,"abstract":"<p><strong>Objectives: </strong>Carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) infections pose significant clinical challenges worldwide due to limited therapeutic options and high mortality. Sulperazon (cefoperazone/sulbactam) has demonstrated activity against multidrug-resistant (MDR) organisms; however, the optimal dosing regimen for CRKP remains unclear. This study aimed to evaluate the clinical efficacy, microbiological outcomes, and safety of different sulperazon dosing in CRKP infections.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 209 adult patients with CRKP infections treated with sulperazon for at least 48 hours at Bengbu First People's Hospital between January 2020 and December 2022. Patients were grouped into a standard-dose group (≤3 g/day, n=112) and a high-dose group (>3 g/day, n=97). Clinical cure, microbiological eradication, treatment failure, 28-day mortality, and incidence of adverse events were compared between groups.</p><p><strong>Results: </strong>The cohort was predominantly elderly (mean age 67.3 ± 15.2 years) and male (65.1%). The most frequent carbapenemase genes were <i>bla</i>_KPC-2 (65.6%) and <i>bla</i>_NDM-1 (23.0%). Virulence factors, including adhesins (72.2%), iron-acquisition systems (63.2%), capsule synthesis genes (58.9%), and the hypermucoviscosity regulator <i>rmpA</i> (42.6%) were frequently detected. Compared with the standard-dose group, the high-dose group showed significantly increased clinical cure rates (64.9% vs. 43.8%; P=0.002) and microbiological eradication rates (69.1% vs. 46.4%; P=0.001), along with lower rates of treatment failure (10.3% vs. 24.1%; P=0.011) and 28-day mortality (8.2% vs. 20.5%; P=0.015), without an increase in adverse events.</p><p><strong>Conclusion: </strong>High-dose sulperazon demonstrated superior clinical efficacy and equivalent safety to standard dosing in the treatment of CRKP infections, supporting dose optimization to improve patient outcomes.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 6","pages":"4764-4773"},"PeriodicalIF":1.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alendronate sodium demonstrates significant clinical advantages in treating osteoporosis secondary to severe fractures.","authors":"Shenggen Wang, Lili Ye, Qiong Hang","doi":"10.62347/MFPV8667","DOIUrl":"10.62347/MFPV8667","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical efficacy of alendronate sodium in patients with osteoporosis secondary to severe fractures.</p><p><strong>Methods: </strong>A total of 102 patients with post-fracture osteoporosis were retrospectively included in this study. The control group (n=45) received standard treatment, while the research group (n=57) was additionally administered alendronate sodium. Bone metabolism markers, pain intensity (assessed by Visual Analogue Scale [VAS]), bone mineral density (BMD), and overall therapeutic efficacy were compared between the two groups. Multivariate logistic regression was performed to identify predictors of therapeutic efficacy.</p><p><strong>Results: </strong>Compared with the control group, the research group demonstrated significantly greater improvements in bone metabolism markers, VAS scores, BMD, and overall efficacy. Univariate and multivariate logistic regression analyses further identified smoking history, alcohol abuse, and treatment modality as independent risk factors for treatment failure, while elevated serum bone Gla protein (BGP) levels were identified as a protective factor.</p><p><strong>Conclusion: </strong>Alendronate sodium significantly improves clinical outcomes in treating patients with osteoporosis secondary to severe fractures.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 6","pages":"4516-4523"},"PeriodicalIF":1.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benefit of electroacupuncture for pulmonary infections in patients with schizophrenia and bipolar disorder: a retrospective study.","authors":"Ping Wang, Yegui Zhang, Li Li","doi":"10.62347/GYLR9082","DOIUrl":"10.62347/GYLR9082","url":null,"abstract":"<p><strong>Objective: </strong>Patients with schizophrenia (SP) and bipolar disorder (BD) who are on prolonged medication are particularly vulnerable to pulmonary infections. This study aims to assess the effectiveness of electroacupuncture (EA) in treating pulmonary infections.</p><p><strong>Methods: </strong>For this retrospective study, data of 170 SP/BD patients with pulmonary infections were collected: a control group (85 patients) and an EA group (85 patients). The control group received standard psychiatric care and supportive treatment, while the EA group also underwent electroacupuncture. After two weeks, various factors including blood oxygen saturation (SpO₂), inflammatory cytokine levels, manic symptoms, and cognitive function were compared between the two groups.</p><p><strong>Results: </strong>Beneficial effects of electroacupuncture on SpO₂ and white blood cell counts were observed after two weeks of intervention. Additionally, electroacupuncture was more effective in reducing inflammatory cytokine levels. Patients receiving electroacupuncture also had lower symptom scores for expectoration, panting, coughing, and sputum compared to the control group (P<0.05). Logistic regression analysis confirmed the important role of electroacupuncture in promoting patient recovery. However, the improvements in manic symptoms and cognitive function with electroacupuncture were not significant compared to the control group (P>0.05).</p><p><strong>Conclusions: </strong>Electroacupuncture can have a beneficial impact on the recovery of SP/BD patients with pulmonary infections.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 6","pages":"4119-4134"},"PeriodicalIF":1.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Body mass index correlates with pain, functional recovery and complications in anterior talofibular ligament arthroscopic surgery.","authors":"Yanping Zhou, Guangchen Sun, Qiliang Lou, Li Zhu","doi":"10.62347/NRVX8467","DOIUrl":"10.62347/NRVX8467","url":null,"abstract":"<p><strong>Objective: </strong>To explore the correlation between body mass index (BMI) and ankle joint function recovery and rehabilitation outcomes in patients with anterior talofibular ligament (ATFL) injury after arthroscopic surgery.</p><p><strong>Methods: </strong>A retrospective study was conducted on 106 ATFL injury patients who underwent arthroscopic ATFL reconstruction in The First People's Hospital of Jiashan from January 2021 to December 2023. Based on BMI, the patients were divided into non-overweight (BMI 18.5-23.9 kg/m²), overweight (BMI 24.0-27.9 kg/m²), and obesity (BMI > 28.0 kg/m²) groups. Intraoperative parameters, preoperative and postoperative pain were measured by visual analogue scale (VAS), ankle function was evaluated by American Orthopedic Foot and Ankle Society (AOFAS) and Carlson Ankle Function Score (KAFS), and postoperative complications were compared among the three groups. The relationship between BMI and postoperative ankle function recovery was analyzed.</p><p><strong>Results: </strong>Postoperative VAS scores decreased remarkably across all three groups, with non-overweight group exhibiting the lowest VAS score, followed by overweight and obesity groups (P < 0.05). AOFAS and KAFS scores notably increased after surgery in all three groups, with non-overweight group demonstrating the most obvious improvement, followed by overweight and obesity groups (P < 0.05). Complications incidence was 4.35%, 17.95%, and 58.62% in non-overweight, overweight, and obesity groups, respectively (P < 0.05). BMI correlated positively with VAS and negatively with AOFAS and KAFS.</p><p><strong>Conclusion: </strong>Higher BMI is associated with more server post-surgical pain, worse ankle function recovery, and poorer rehabilitation outcomes.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 6","pages":"4288-4296"},"PeriodicalIF":1.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive value of quantitative DNA analysis in the carcinogenesis of oral submucous fibrosis.","authors":"Yi Yang, Keke Zhu","doi":"10.62347/HMPQ6800","DOIUrl":"10.62347/HMPQ6800","url":null,"abstract":"<p><strong>Objective: </strong>To explore the diagnostic value of DNA quantitative analysis for the malignant transformation of Oral submucous fibrosis (OSF), as well as the risk factors for OSF carcinogenesis.</p><p><strong>Methods: </strong>Clinical data from 85 OSF patients treated between June 2021 and June 2023 were retrospectively analyzed. Using pathological diagnosis as the gold standard, the diagnostic performance of DNA quantification was evaluated. The relationship between DNA quantitative findings and patients clinicopathological features was analyzed. Meanwhile, factors associated with abnormal DNA content and risk factors for OSF malignant transformation were identified through multivariate logistic regression analysis. The receiver operating characteristic curve (ROC) was employed to evaluate the diagnostic performance of DNA quantitative analysis for predicting malignant transformation.</p><p><strong>Results: </strong>Among the 85 OSF patients, 34 (40.0%) developed malignant transformation. DNA quantitative analysis results revealed 40 (47.1%) had abnormal DNA content, while the other 45 did not. Multivariate Logistic regression analysis identified lesion location and degree of epithelial dysplasia as risk factors for abnormal DNA content; betel-nut chewing frequency, smoking, and the presence of oral leukoplakia (OLK) or oral lichen planus (OLP) were significantly associated with OSF carcinogenesis. The ROC curve for DNA quantitative analysis in diagnosing OSF carcinogenesis showed an area under the curve (AUC) of 0.770, with a sensitivity of 79.4%, specificity of 74.5%, positive predictive value of 67.5%, and negative predictive value of 84.4%.</p><p><strong>Conclusion: </strong>DNA quantitative analysis is a valuable tool for assessing carcinogenic risk of OSF. Lesion location and epithelial dysplasia are significant factors associated with abnormal DNA content, while lifestyle factors and coexisting oral lesions contribute to malignant transformation risk.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 6","pages":"4278-4287"},"PeriodicalIF":1.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12261170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}