American journal of translational research最新文献

{"title":"Erratum: PAK2 promotes migration and proliferation of salivary gland adenoid cystic carcinoma.","authors":"Wei-Wei Deng, Lei Wu, Lin-Lin Bu, Jian-Feng Liu, Yi-Cun Li, Si-Rui Ma, Guang-Tao Yu, Liang Mao, Wen-Feng Zhang, Zhi-Jun Sun","doi":"10.62347/XRJR2507","DOIUrl":"10.62347/XRJR2507","url":null,"abstract":"<p><p>[This corrects the article on p. 3387 in vol. 8, PMID: 27648129.].</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6688-6693"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coagulation biomarkers as predictors of transfusion outcomes in trauma patients.","authors":"Yude Jin, Liping Fei","doi":"10.62347/ZZBC1816","DOIUrl":"10.62347/ZZBC1816","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the role of coagulation biomarkers (fibrinogen [FIB], fibrin degradation products [FDP], D-dimer [D-D], FDP/FIB ratio) in predicting transfusion outcomes in trauma patients.</p><p><strong>Methods: </strong>A retrospective analysis of 112 trauma cases (May 2020-May 2024) stratified into good (n=80) and poor prognosis (n=32) groups based on transfusion outcomes was conducted. Pre-transfusion levels of coagulation biomarkers were compared between groups. Pearson correlation assessed associations among markers, and logistic regression identified outcome predictors. Clinical parameters, including blood pressure, complete blood count, and coagulation function, were also considered. The predictive value was evaluated through receiver operating characteristic curve analysis.</p><p><strong>Results: </strong>The poor prognosis group exhibited lower FIB but higher FDP, D-D, FDP/FIB ratio, and white blood cell count (WBC) (all P<0.01). Additionally, this group had longer prothrombin time and activated partial thromboplastin time (both P<0.01) as well as greater plasma transfusion volumes (P<0.05). An inverse relationship was identified between FIB and FDP/D-D levels across prognosis groups. However, positive FDP/D-D correlation was observed only in the poor prognosis group, with no significant FDP/D-D linkage found in the good prognosis group; moreover, these correlations were stronger in cases with worse clinical outcomes. Multivariate analysis identified FIB, FDP, D-D, and WBC as independent predictors. The combined biomarker model (area under the curve (AUC) =0.923) outperformed individual markers (AUC range: 0.691-0.809).</p><p><strong>Conclusion: </strong>FIB, FDP, D-D, and WBC are significant predictors of transfusion outcomes in trauma patients. A combined biomarker model demonstrates superior predictive performance, highlighting the importance of identifying coagulation dysregulation in trauma prognosis.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6381-6390"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application and limitations of ultrasound for the early diagnosis of thyroid cancer: a systematic review and meta-analysis.","authors":"Yanshen Liu, Weiming Xu","doi":"10.62347/BNBG2830","DOIUrl":"10.62347/BNBG2830","url":null,"abstract":"<p><strong>Objective: </strong>To systematically evaluate the diagnostic performance of high-frequency ultrasound and its sonographic features in the early detection of thyroid cancer through a meta-analysis.</p><p><strong>Methods: </strong>A comprehensive search was conducted in PubMed, Embase, and Web of Science for studies published up to December 31, 2024. Studies assessing the diagnostic performance of high-frequency ultrasound and ultrasound-guided procedures for thyroid cancer were included based on predefined criteria. Extracted data included sensitivity, specificity, and the diagnostic relevance of sonographic features (e.g., nodule size, margin irregularity, echogenicity, calcifications, and vascularity). Study quality was assessed using the Quality Assessment of Diagnostic Accuracy Studies (QUADAS) tool. Meta-analytic methods were applied to pool diagnostic accuracy measures. Publication bias was evaluated using funnel plots.</p><p><strong>Results: </strong>A total of 14 studies were included. Combined diagnostic approaches, particularly high-frequency ultrasound combined with ultrasound-guided fine-needle aspiration biopsy (US-FNAB), demonstrated high sensitivity (ranging from 0.814 to 0.975) and specificity (ranging from 0.833 to 0.976). Key sonographic features significantly associated with malignancy included hypoechogenicity, microcalcifications, and irregular margins. Pooled analysis showed that microcalcifications and irregular margins were strong predictors for malignancy, with an overall Peto odds ratios (OR) of 39.47 [28.88, 53.94] for irregular margins (P<0.001). Minimal publication bias was observed for most features, although mild asymmetry was noted in analyses involving microcalcifications.</p><p><strong>Conclusion: </strong>High-frequency ultrasound, particularly when combined with ultrasound-guided biopsy or contrast-enhanced ultrasound, provides high diagnostic accuracy for thyroid cancer. Specific features, such as hypoechogenicity, microcalcifications, and irregular margins, are valuable in differentiating malignant from benign thyroid nodules. Future studies should aim to standardize ultrasound-based diagnostic criteria and explore the use of multimodal imaging techniques to improve early thyroid cancer detection.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6556-6572"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interplay between endothelial cell dysfunction and podocyte injury in diabetic nephropathy: a comprehensive review of current evidence.","authors":"Shuai Guo, Hong-Min Luo, Ling-Ling Wang, Xin-Ai Huo, Yan-Qing Chi","doi":"10.62347/QQMX6273","DOIUrl":"10.62347/QQMX6273","url":null,"abstract":"<p><p>Diabetic nephropathy (DN) remains the leading cause of end-stage renal disease globally. Emerging evidence highlights the bidirectional crosstalk between glomerular endothelial cell (GEC) dysfunction and podocyte injury as a key driver of DN progression. This review synthesizes current understanding of the molecular mechanisms, clinical correlations, and therapeutic strategies targeting this interplay. Mechanistically, hyperglycemia-induced oxidative stress, dysregulated angiogenesis, and aberrant extracellular vesicle (EV)-mediated signaling contribute to a self-perpetuating cycle of glomerular injury. Clinically, biomarkers of endothelial-podocyte axis disruption predict disease progression and therapeutic response. Novel therapies, including endothelin receptor antagonists, sodium-glucose cotransporter 2 (SGLT2) inhibitors, and mesenchymal stem cell derived EVs, show promise for restoring glomerular filtration barrier (GFB) integrity. This review integrates multi-omics insights to propose a unified model of DN pathogenesis and precision medicine approaches.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"5862-5870"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NLBK alleviates airway remodeling in COPD by inhibiting RXRA-mediated transcription of PLA2G2A.","authors":"Teng Zhang, Fang Fang, Dan Wu, Guodong Wang","doi":"10.62347/DGOC8275","DOIUrl":"10.62347/DGOC8275","url":null,"abstract":"<p><strong>Objectives: </strong>Nourishing lung benefiting kidney granule (NLBK) is used to treat chronic obstructive pulmonary disease (COPD). However, the molecular mechanism underlying its therapeutic effect is still unclear. In this study, we elucidated the molecular mechanism by which NLBK alleviates airway reorganization in COPD.</p><p><strong>Methods: </strong>We investigated the function of NLBK in regulating inflammatory reactions and endoplasmic reticulum stress (ERS) in mice with COPD. Through bioinformatics and network pharmacology analysis, we identified the main components and targets: Zhebeiresinol-nuclear receptors such as Retinol X receptor A (RXRA) and Phospholipase A2, group IIA (PLA2G2A). A rescue experiment was performed to confirm the relationship between Zhebeiresinol and PLA2G2A in alleviating COPD symptoms. Moreover, by conducting a series of experiments, we determined the transcriptional regulation of RXRA on PLA2G2A.</p><p><strong>Results: </strong>NLBK significantly inhibited cigarette smoke exposure-induced inflammatory response, lung function injury, and ERS in COPD mice. Zhebeiresinol acted as an active ingredient of NLBK, which was found to mitigate the inflammatory response, lung function injury, and ERS in COPD mice through the silencing of PLA2G2A, the specific target of NLBK. Zhebeiresinol repressed the phosphorylation of RXRA and entry into the nucleus, which efficiently suppressed the transcription of PLA2G2A.</p><p><strong>Conclusions: </strong>NLBK can alleviate airway remodeling in COPD through the RXRA-PLA2G2A axis, providing a new mechanistic basis for the clinical application of NLBK.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6504-6521"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knockdown of TIM1 enhances platinum chemosensitivity and inhibits progression through the MAPK signaling pathway in gastric cancer.","authors":"Congkai Zhang, Chao Zhu, Haoxuan Huang, Mengwei Liu, Kuai Yu, Aiping Le","doi":"10.62347/VAFF5386","DOIUrl":"10.62347/VAFF5386","url":null,"abstract":"<p><strong>Objectives: </strong>The incidence of gastric cancer (GC) is increasing worldwide, and it ranks among the leading causes of cancer-related mortality. Platinum-based chemotherapeutic agents are commonly employed in the treatment of various solid tumors; however, the development of drug resistance remains a crucial barrier to effective treatment. T cell immunoglobulin and mucin domain 1 (TIM1) has been implicated in the initiation and progression of various tumors. Nevertheless, the influence of TIM1 on the efficacy of platinum-based chemotherapeutics and its biologic implications in GC have not been thoroughly investigated.</p><p><strong>Methods: </strong>This study utilized flow cytometry, cell counting kit-8 assays, and western blot to assess the role of TIM1 in modulating sensitivity to platinum drugs. High-throughput sequencing was employed to elucidate the potential mechanism of TIM1. Additionally, the effect of TIM1 on the biologic characteristics of GC was further investigated through clinical specimens, cells, and animals.</p><p><strong>Results: </strong>Knockdown of TIM1 enhanced the sensitivity of GC cells to platinum chemotherapeutic drugs. Furthermore, TIM1 expression was elevated in GC tissues, and high TIM1 expression predicted poor survival outcomes in GC patients. Knockdown of TIM1 inhibited the malignant behaviors of GC cells in vitro and in vivo. The mitogen-activated protein kinase (MAPK) signaling pathway may play a role in the regulatory effects of TIM1 on GC cells.</p><p><strong>Conclusions: </strong>Overall, TIM1 functions as an oncogene in GC. Knockdown of TIM1 enhances platinum chemosensitivity and inhibits malignant behavior in GC through the MAPK signaling pathway. These findings reveal a molecular mechanism contributing to chemotherapy resistance and suggest a therapeutic strategy for enhancing chemotherapy.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6028-6041"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a machine learning-based predictive model for intraoperative hypothermia risk during radical surgery for oral cancer.","authors":"Hao Duan, Haoling Liu, Weiwei Liu, Yuan Zhang, Pengying Yan, Baolei Wu, Yiwei Ma","doi":"10.62347/RIGS6581","DOIUrl":"10.62347/RIGS6581","url":null,"abstract":"<p><strong>Objective: </strong>To develop and validate a machine learning (ML)-based model for predicting the risk of intraoperative hypothermia in patients undergoing radical oral cancer surgery and to identify key contributing risk factors for clinical reference.</p><p><strong>Methods: </strong>This retrospective study included 402 patients who underwent radical oral cancer resection, divided into training (n = 281) and validation (n = 121) cohorts. Demographic data, physiologic indicators, and intraoperative variables were collected. Predictive models were constructed using Least Absolute Shrinkage and Selection Operator (LASSO) regression, eXtreme Gradient Boosting (XGBoost), and Random Forest (RF) algorithms. Model performance was evaluated using receiver operating characteristic curves, calibration plots, and Shapley Additive Explanations (SHAP) analysis.</p><p><strong>Results: </strong>The RF model demonstrated superior performance, achieving an area under the curve (AUC) of 0.821 (95% confidence interval [CI]: 0.783-0.856) in the training cohort and 0.807 (95% CI: 0.742-0.865) in the validation cohort, with 64.6% sensitivity. It outperformed both the XGBoost model (validation AUC = 0.721) and LASSO model (validation AUC = 0.738). SHAP analysis identified surgical duration > 441 minutes (odds ratio [OR] = 2.31), baseline temperature ≤ 36.5°C (OR = 3.12), and intraoperative fluid volume ≥ 4.6 liters (OR = 1.89) as the most important predictors. Calibration curves showed strong agreement between predicted and actual outcomes (mean absolute error = 0.17).</p><p><strong>Conclusion: </strong>The ML-based RF model provides reliable prediction of intraoperative hypothermia risk in oral cancer surgery. Surgical duration and baseline temperature emerged as key risk factors, offering targets for perioperative risk stratification and intervention.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6303-6319"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Latrophilin-1 and latrophilin-2 as androgen receptor-responsive G protein-coupled receptors promote bladder cancer progression.","authors":"Takuro Goto, Taro Akai, Yuki Teramoto, Hiroshi Miyamoto","doi":"10.62347/KFGI9710","DOIUrl":"10.62347/KFGI9710","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the functional role of latrophilin-1 (LPHN1; encoded by the <i>ADGRL1</i> gene) and latrophilin-2 (LPHN2; encoded by the <i>ADGRL2</i> gene), members of the G protein-coupled receptor family, in relation to androgen receptor (AR) signaling, in the outgrowth of bladder cancer.</p><p><strong>Methods: </strong>Human bladder urothelial carcinoma cell lines were subjected to real-time PCR, western blotting, chromatin immunoprecipitation, MTT assay, and wound-healing assay. Immunostaining was also performed on a set of bladder cancer tissue microarrays consisting of transurethral resection specimens.</p><p><strong>Results: </strong>In bladder cancer cells with endogenous or exogenous AR expression, dihydrotestosterone markedly up-regulated <i>ADGRL1</i>/LPHN1 and <i>ADGRL2</i>/LPHN2 expression. Chromatin immunoprecipitation confirmed AR binding to the promoter regions of <i>ADGRL1</i> and <i>ADGRL2</i>. Additionally, LPHN ligands (<i>e.g.</i> α-latrotoxin, FLRT3) induced their expression. Knockdown of LPHN1 or LPHN2 via shRNA virus infection significantly reduced cell viability and migration, while the stimulatory effects of LPHN ligands on cell viability were more significant in AR-negative or AR-knockdown lines than in corresponding AR-positive lines. Immunohistochemical analysis in surgical specimens further showed that LPHN1 overexpression (<i>i.e.</i> moderate/strong) in muscle-invasive tumors (n = 62) independently predicted poorer disease-specific survival following radical cystectomy (hazard ratio 2.662, <i>P</i> = 0.031). Analysis of The Cancer Genome Atlas (TCGA) dataset (n = 305, stage II-IV bladder cancer) also revealed that high <i>ADGRL2</i> expression was associated with significantly worse overall survival.</p><p><strong>Conclusion: </strong>These findings suggest that LPHN1 and LPHN2 function as downstream effectors of AR and contribute to the progression of bladder cancer.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6403-6413"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics of primary pulmonary hemangiosarcoma outcomes: a Surveillance, Epidemiology, and End Results database analysis.","authors":"Jia-Chun Sun, Zi-Han Yang, Hao-Lin Shi, Ting-Ting Wei, Bo Sun, Jing-Xiang Su, Hong-Yan Liu, Qing-Feng Wang, Xin-Yang Li","doi":"10.62347/VHRY6983","DOIUrl":"10.62347/VHRY6983","url":null,"abstract":"<p><p>Primary pulmonary hemangiosarcoma (PPHS) is an extraordinarily rare disorder. The objective of this study was to investigate the demographic characteristics and prognostic factors of patients with PPHS. Patients diagnosed with PPHS between 2000 and 2020 based on data from the Surveillance, Epidemiology, and End Results (SEER) database were retrospectively analyzed. Survival analysis was performed using the survival package of R (4.2.0). Univariate and multivariate Cox proportional hazards models were used to analyze independent prognostic factors. A nomogram was constructed by the R Regression Modeling Strategies (rms) package. A total of 123 patients with PPHS were included in this study; their mean survival time was 13.8 months. Further investigation revealed that the disease-specific survival rates of patients with PPHS at 1, 2, 3, and 5 years were 20.17%, 14.29%, 10.08%, and 5.88% respectively. In the regression analysis, age, tumor status, and treatment were identified as risk factors for patients with PPHS. In addition, chemotherapy was crucial for the treatment of patients with PPHS (P < 0.05). Finally, the nomogram we constructed to predict 0.5-, 1-, 2-, and 3-year disease-specific survival in patients with PPHS showed good accuracy (concordance index: 0.769). Age, tumor laterality, and tumor size were independent factors affecting the prognosis of patients with PPHS, and chemotherapy may significantly improve the long-term prognosis.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6191-6202"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-124 inhibits the proliferation, migration and invasion of esophageal squamous cell carcinoma by downregulating STATS.","authors":"Baolong Ding, Weiqian Wang, Ruijuan Sun, Can Sun, Xu Yang, Chunbo Zhai","doi":"10.62347/RSNT9484","DOIUrl":"10.62347/RSNT9484","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the expression, role and the underlying mechanism of miR-124 in esophageal squamous cell carcinoma (ESCC).</p><p><strong>Methods: </strong>A total of 25 pairs of ESCC and adjacent tissues were collected for analysis. The human normal esophageal epithelial cell line (Het-1A) and human esophageal cancer cell lines (EC-1) was cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS). Cells were transfected with miR-124 mimics and inhibitors. A series of assays, including Cell-Counting-Kit-8 (CCK-8), Transwell migration and invasion assays, real-time quantitative polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay (ELISA) and luciferase reporter gene assay, were performed to detect the effects of miR-124 on ESCC cells. Spearman's correlation analysis was used to evaluate the relationship of miR-124 expression and signal transducer and activator of transcription-3 (STAT3) expression.</p><p><strong>Results: </strong>miR-124 was significantly downregulated in ESCC tissues and cells. Overexpression of miR-124 inhibited the proliferation, migration and invasion of ESCC cells in vitro, while inhibition of miR-124 promoted these processes (all P<0.05). miR-124 was found to specifically bind to the 3'-untranslated region (3'UTR) of STAT3. The expression level of STAT3 was obviously higher in tumor tissues and cells compared to normal adjacent tissues and cells (all P<0.05). Moreover, miR-124 expression was negatively associated with STAT3 levels. Restoring STAT3 expression in ESCC cells transfected with miR-124 mimics partially reversed the inhibitory effects of miR-124 mimics on cell proliferation, migration, and invasion. Conversely, inhibiting STAT3 expression in ESCC cells transfected with miR-124 inhibitors partially abolished the promoting effects of miR-124 inhibitors on the proliferation, migration and invasion of ESCC cells.</p><p><strong>Conclusion: </strong>miR-124 inhibits the proliferation, migration and invasion of ESCC cells by downregulating STATS, indicating that miR-124 may serve as a molecular candidate for treating ESCC.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"5939-5948"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}