American journal of translational research最新文献

{"title":"Effect of percutaneous vertebroplasty on the treatment of osteoporotic spinal fractures in elderly patients and risk factors for postoperative lower extremity deep vein thrombosis.","authors":"Jinpeng Chen, Gaochen Wu, Yiqi Miao, Lulu Wang, Fanjian Meng","doi":"10.62347/XELD8876","DOIUrl":"https://doi.org/10.62347/XELD8876","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effectiveness of PVP (PVP) in treating osteoporotic spinal fractures in the elderly and analyze the risk factors for postoperative deep vein thrombosis (DVT) in the lower extremities.</p><p><strong>Methods: </strong>A total of 100 elderly patients with osteoporotic spinal fractures, treated between August 2019 and July 2021, were divided into two groups: PVP (research group, n=50) and conservative treatment (control group, n=50). Outcome measures, including injured vertebrae, pain levels, and treatment outcomes, were retrospectively analyzed. Patients who underwent PVPs were further categorized based on the presence of lower extremity DVT one month post-surgery. Logistic regression analysis was used to identify risk factors for post-surgical lower limb DVT.</p><p><strong>Results: </strong>PVP resulted in a significantly smaller posterior convexity angle of the injured spine and higher anterior, midline, and posterior edges of the injured spine compared to conservative treatment (P<0.001). Patients in the research group had significantly lower visual analogue scale (VAS) scores and higher treatment efficiency compared to those in the conservative treatment group (all P<0.05). Nine cases of lower extremity DVT were observed after PVP. Logistic regression analysis identified age, body mass, smoking, and diabetes as independent risk factors for post-surgical lower extremity DVT.</p><p><strong>Conclusion: </strong>PVP improves spinal function and relieves pain in elderly patients with osteoporotic fractures. However, age, body mass, smoking, and diabetes are independent risk factors for postoperative lower extremity DVT.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of AFP-L3 and Des-γ-carboxy prothrombin in advanced primary liver cancer treated with Sorafenib and transarterial chemoembolization.","authors":"Shiwen Tang, Hao Liu, Peiyang Chen, Jiaqing He, Haiwei Chen, Jingqi Chen, Yanli Liu","doi":"10.62347/PMYP4404","DOIUrl":"https://doi.org/10.62347/PMYP4404","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the prognostic value of serum alpha-fetoprotein (AFP-L3) and Des-γ-carboxy prothrombin (DCP) in advanced primary liver cancer (PLC) undergoing combined treatment with Sorafenib and transarterial chemoembolization (TACE).</p><p><strong>Methods: </strong>This retrospective analysis included 82 patients with advanced PLC treated at the Second Affiliated Hospital, Guangzhou Medical University from January 2018 to January 2020. The patients were divided into an observation group (41 cases) and a control group (41 cases) based on their treatment method. The control group received TACE, while the observation group received a combination of Sorafenib and TACE. Both groups were evaluated after 12 weeks of treatment. Serum AFP-L3 and DCP levels were measured using a chemiluminescence immunoassay with magnetic particles. Short-term efficacy was compared between the two groups after 12 weeks of treatment. Additionally, Karnofsky Performance Status (KPS) scores, serum AFP-L3 and DCP levels before and after 12 weeks of treatment, and the survival rate after 2 years of follow-up were recorded. Serum AFP-L3 and DCP levels were compared between surviving and deceased patients.</p><p><strong>Results: </strong>The objective response rate in the observation group (68.29%) was higher than in the control group (46.34%) (P<0.05). KPS scores in both groups were significantly higher 12 weeks post-treatment compared to pre-treatment (P<0.05); the observation group had higher post-treatment KPS scores than the control group (P<0.05). Serum AFP-L3 and DCP levels were reduced in both groups after 12 weeks of treatment compared to pre-treatment levels (P<0.05). However, post-treatment serum AFP-L3 and DCP levels were lower in the observation group compared to the control group (both P<0.05). After 2 years of follow-up, the survival rate was higher in the observation group compared to the control group (P<0.05). AFP-L3 and DCP levels were higher in deceased patients compared to surviving patients after 2 years of follow-up (both P<0.05).</p><p><strong>Conclusion: </strong>Combination therapy with Sorafenib and TACE is effective for patients with advanced PLC, reducing AFP-L3 and DCP levels and improving patient survival rates. Additionally, higher levels of serum AFP-L3 and DCP are associated with poor prognosis.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between cognitive impairment and body composition indicators in patients with chronic kidney disease after hemodialysis.","authors":"Dan Yuan, Zhenxing Sun, Jingjing Zhou, Xiaoqi Wang, Zhongxin Li","doi":"10.62347/HFGL5701","DOIUrl":"https://doi.org/10.62347/HFGL5701","url":null,"abstract":"<p><strong>Objective: </strong>To examine the relationship between cognitive impairment and body composition indicators in chronic kidney disease (CKD) patients post-hemodialysis.</p><p><strong>Methods: </strong>This retrospective study included 110 CKD patients admitted to Beijing Luhe Hospital, Capital Medical University between January 2019 and January 2023. General clinical data and body composition indicators were compared between patients with and without cognitive impairment. Multiple logistic regression and ROC curve analysis were used to identify influencing factors and to develop a predictive model.</p><p><strong>Results: </strong>Cognitive impairment occurred in 50% of the patients post-hemodialysis. No significant differences were found in demographics, disease duration, comorbidities, or hemodialysis duration between the groups (all P > 0.05). However, significant differences were observed in body mass index (BMI) (P < 0.001), lean body mass index (LTI) (P = 0.007), fat tissue index (FTI) (P = 0.024), and total body water (TBW) (P < 0.001). Multiple logistic regression identified TBW (OR 4.900, 95% CI 3.062-7.511, P < 0.001), the TBW/extracellular water (ECW) ratio (OR 7.244, 95% CI 5.092-8.7613, P = 0.016), and the ECW/body cell mass (BCM) ratio (OR 6.720, 95% CI 4.564-8.692, P = 0.030) as independent risk factors for cognitive impairment post-hemodialysis. ROC analysis confirmed their predictive capacity, with AUC values of 0.840, 0.840, and 0.850 respectively. A predictive model incorporating these indicators was developed, showing good calibration (Hosmer-Lemeshow test, P = 0.912) and discrimination (C-index 0.974, 95% CI 0.952-0.997).</p><p><strong>Conclusion: </strong>Total body water, the TBW/ECW ratio, and the ECW/BCM ratio are independently associated with cognitive impairment in CKD patients post-hemodialysis. Body composition analysis serves as a valuable tool for predicting cognitive impairment in this population, guiding clinicians in assessing cognitive function and planning interventions for these patients.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early use of SGLT2 inhibitors reduces the progression of diabetic kidney disease: a retrospective cohort study.","authors":"Shaowei Pang, Xiaoli Li","doi":"10.62347/ARYA8831","DOIUrl":"https://doi.org/10.62347/ARYA8831","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the potential of sodium-glucose cotransporter 2 (SGLT2) inhibitors in preventing the progression of diabetic kidney disease and to provide guidance for clinical practice to improve renal health management strategies for diabetic patients.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 178 patients with diabetic kidney disease admitted to Baoji High Tech Hospital from March 2023 to March 2024. Of these, 88 patients who received early treatment with the SGLT2 inhibitor dapagliflozin were included in the early SGLT2-i group, while 90 patients receiving later treatment with SGLT2 inhibitor dapagliflozin were included in the late SGLT2-i group. Clinical data, overall effectiveness, adverse reactions, blood glucose, renal function, lipid levels, and inflammatory markers were compared between the two groups.</p><p><strong>Results: </strong>Prior to treatment, there were no differences in blood glucose indicators between the two groups (all P > 0.05). Following treatment, both groups showed reductions in 2-hour postprandial blood glucose (2hPG), fasting plasma glucose (FPG), and glycosylated hemoglobin (HbA1c), with the early SGLT2-i group demonstrating significantly lower values compared to the late SGLT2-i group (all P < 0.05). Similarly, there were no differences in renal function indicators between the two groups before treatment (all P > 0.05). However, following treatment, the early SGLT2-i group showed more noticeable improvements compared to the late SGLT2-i group (P < 0.05). Inflammatory markers and lipid levels followed similar patterns. The overall effectiveness of the early SGLT2-i group was higher than that of the late SGLT2-i group (92.05% vs. 78.89%, P < 0.05), while the incidence of adverse reactions did not differ statistically between the two groups (6.82% vs. 10.00%, P > 0.05).</p><p><strong>Conclusion: </strong>Early use of SGLT2 inhibitors in diabetic kidney disease patients effectively controls blood glucose and lipid levels, improves renal function, reduces inflammatory responses, and exhibits a low incidence of adverse reactions. This demonstrates high safety and an important role in delaying disease progression. Therefore, it is worth considering clinical promotion and use for this patient population.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of repeated low-level red-light therapy combined with optical lenses for myopia control in children and adolescents.","authors":"Gangyue Wu, Xiaodan Dai, Junchi Tian, Jingru Sun","doi":"10.62347/DTLF6342","DOIUrl":"https://doi.org/10.62347/DTLF6342","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the efficacy of repeated low-level red-light (RLRL) therapy combined with optical lenses in children and adolescents with myopia.</p><p><strong>Methods: </strong>This retrospective study included 108 children and adolescents. Based on the difference in the combination intervention scheme participants were divided into four groups based on the intervention they received: the RLRL+orthokeratology (OK) lens intervention group (RLRL+OK group), the RLRL+defocus distributed multi-point (DDM) lens intervention group (RLRL+DDM group), the RLRL+single-vision spectacles (SVS) intervention group (RLRL+SVS group), and a control group. Visual acuity, spherical equivalent refraction (SER), and axial length (AL) were measured before and after the intervention. Binary logistic regression was used to identify factors influencing vision recovery.</p><p><strong>Results: </strong>The SER and AL at baseline were statistically different (<i>P</i><0.01). After the intervention, the AL increase in the RLRL+OK, RLRL+DDM, and RLRL+SVS groups was significantly better than the control group across time points (<i>P</i><0.001). Changes in SER were also statistically significant in the RLRL+DDM and RLRL+SVS groups compared to the control group across time points (<i>P</i><0.001). The intervention method was identified as a significant factor influencing vision recovery (<i>P</i><0.001).</p><p><strong>Conclusion: </strong>RLRL therapy combined with optical lenses is effective in controlling myopia progression in children and adolescents.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for varus knee osteoarthritis and their relationship with disease severity based on Kellgren-Lawrence classification.","authors":"Xiaohua Xue, Jianchi Li, Yuanqing Liang, Fanfan Cai, Zhongwei Zhao, Gang Liu","doi":"10.62347/YMJV4880","DOIUrl":"https://doi.org/10.62347/YMJV4880","url":null,"abstract":"<p><strong>Objective: </strong>To explore the hazard element of varus knee osteoarthritis (OA) and its relationship with Ailment severity based on the Kellgren-Lawrence classification.</p><p><strong>Methods: </strong>This study selected a cohort of patients who initially presented without clinical signs of arthritis and did not exhibit knee valgus or varus deformities when assessed from a hip-knee-ankle alignment perspective, focusing on the internal side. As the study progressed some individuals developed OA. Eligible participants were those who had undergone a standard whole lower extremity weight-bearing X-ray examination at our orthopedic outpatient clinic. The study period spanned from January 2021 to April 2022, and the selection criteria were strictly applied throughout this timeframe. Data on clinical and X-ray indices, including fibular head height, Kellgren-Lawrence grade, joint line convergence angle (JLCA), proximal medial tibial angle (MPTA), and Hip-knee-ankle angle, were collected from patients.</p><p><strong>Results: </strong>The study included 23 grade 0, 22 grade I, 32 grade II, 32 grade III, and 97 grade IV Kellgren-Lawrence classified patients. Significant differences in age, gender, and BMI were observed across groups (all P<0.05). With increasing OA severity, fibular head height and MPTA decreased, while JLCA and Hip-knee-ankle angle increased significantly (all P<0.05). Patients with OA exhibited larger fibular head height and MPTA but smaller JLCA and Hip-knee-ankle angle than those without arthritis (all P<0.05). Logistic regression analysis confirmed age, fibular head height, MPTA, JLCA, and Hip-knee-ankle angle as independent risk factors for varus knee OA (all P<0.05). Additionally, there was a strong positive correlation between age and fibular head height with disease severity, but a strong negative correlation between Hip-knee-ankle angle and disease severity (all P<0.05).</p><p><strong>Conclusion: </strong>Patients with varus knee OA have larger fibular head height and MPTA than those without arthritis. Age, fibular head height, and Hip-knee-ankle angle are risk factors for the development of varus knee OA. The severity of arthritis and varus deformity increases with age and fibular head height.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of internet-based integrated care on breastfeeding outcomes and emotional well-being in primiparous women.","authors":"Xiaoting Shi, Chunhua Liu, Xueping Yang, Xianghong Liu, Xinyun Hui","doi":"10.62347/UMKH2239","DOIUrl":"https://doi.org/10.62347/UMKH2239","url":null,"abstract":"<p><strong>Objective: </strong>This study investigates the impact of Internet-based integrated care on breastfeeding outcomes and emotional well-being in primiparous women.</p><p><strong>Methods: </strong>We conducted a retrospective analysis of 154 primiparous women admitted to Baoji People's Hospital from July 2020 to September 2023. Participants were divided into an observation group (n = 58), which received internet-based integrated care, and a control group (n = 96), which received routine telephone follow-up. We compared breastfeeding outcomes, knowledge, self-efficacy, and infant physical development between the groups, and performed logistic regression to identify risk factors affecting breastfeeding.</p><p><strong>Results: </strong>Before the intervention, there were no significant differences in breastfeeding knowledge and self-efficacy scores between the groups (all P > 0.05). At 6 months postpartum, the observation group had a higher breastfeeding success rate and improved scores in breastfeeding knowledge and self-efficacy (all P < 0.05). Infants in the observation group exhibited better physical development compared to those in the control group (P < 0.05). Logistic regression analysis identified age (P = 0.019), mode of delivery (P = 0.006), and nipple condition (P = 0.029) as independent risk factors for exclusive breastfeeding, while education level (P < 0.001), type of employment (P = 0.002), and daily sucking frequency (P = 0.005) were identified as independent protective factors.</p><p><strong>Conclusions: </strong>The Internet-based integrated care model significantly enhances breastfeeding knowledge, self-efficacy, and exclusive breastfeeding rates among primiparous women, while also supporting better infant physical development.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of SIRT6 and VNN1 in children with primary nephrotic syndrome and their correlation with acute kidney injury.","authors":"Fang Hao, Shujun Zhang, Yi Gao","doi":"10.62347/TOUH6376","DOIUrl":"https://doi.org/10.62347/TOUH6376","url":null,"abstract":"<p><strong>Objective: </strong>To explore the diagnostic and prognostic values of Sirtuin 6 (SIRT6) and Vanin-1 (VNN1) in peripheral blood monocytes of children with primary nephrotic syndrome (PNS) and their correlation with acute kidney injury (AKI).</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 101 children (observation group) diagnosed with PNS and treated at the Shanxi University of Traditional Chinese Medicine Affiliated Hospital from December 2021 to December 2023. These children were categorized into two groups: the AKI group (n=35) and the non-AKI group (n=66), based on the presence of AKI. Additionally, 101 healthy children who underwent physical examinations during the same period served as the control group. Western blotting and RT-PCR were employed to measure the protein and mRNA levels of SIRT6 and VNN1 in monocytes across the three groups. The correlation between SIRT6 and VNN1 mRNA levels and clinical data, as well as kidney function indicators, was analyzed. The diagnostic value of SIRT6 and VNN1 mRNA levels for AKI in PNS was assessed using ROC curves. Multivariate logistic regression identified independent factors influencing AKI in PNS. The mRNA levels of SIRT6 and VNN1 were also compared before and after treatment in children with PNS.</p><p><strong>Results: </strong>The AKI group exhibited lower SIRT6 protein and mRNA levels, and higher VNN1 protein and mRNA levels in monocytes compared to the other groups (all P<0.05). Correlation analysis revealed that SIRT6 mRNA levels were positively correlated with serum creatinine (Scr), uric acid (UA), blood urea nitrogen (BUN), 24-hour urine protein (24h UP), cystatin C (Cys-C), and β2-microglobulin (β2-MG), but negatively correlated with albumin (ALB) and estimated glomerular filtration rate (eGFR) (all P<0.05). In contrast, VNN1 levels showed the opposite correlations (P<0.05). ROC curve analysis showed that the AUC for SIRT6 or VNN1 mRNA alone in diagnosing AKI was above 0.8, with a combined diagnostic AUC exceeding 0.9. Logistic regression indicated that eGFR, β2-MG, Cys-C, and the mRNA levels of SIRT6 and VNN1 were independent risk factors for AKI in PNS (all P<0.05). After treatment, SIRT6 mRNA levels significantly decreased, while VNN1 mRNA levels increased in children with PNS (both P<0.05).</p><p><strong>Conclusion: </strong>SIRT6 and VNN1 are closely associated with AKI in children with PNS and may serve as valuable biomarkers for the diagnosis of AKI.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CB2-PKC pathway is involved in esketamine-induced anti-inflammation in BV-2 microglial cells exposed to lipopolysaccharides.","authors":"Yuqing Wang, Ming Cao, Yuanyuan Zhang, Qian Chen, Zhaojie Chen, Ji Jia","doi":"10.62347/RRZF5229","DOIUrl":"https://doi.org/10.62347/RRZF5229","url":null,"abstract":"<p><strong>Objective: </strong>Esketamine (ESK), an intravenous anesthetic, exerts antidepressant effects; however, the antidepression mechanism is not clear. The aim of this study was to explore whether microglial cannabinoid type 2 (CB2) receptor and protein kinase C (PKC) are involved in the antidepressant effects of ESK.</p><p><strong>Methods: </strong>In this investigation, lipopolysaccharide (LPS) was used to stimulate BV-2 microglia to mimic neuroinflammation. An enzyme-linked immunosorbent assay (ELISA) and Griess reagent kits were used to determine cytokine and nitrite concentrations in the medium. CB2, inducible nitric oxide synthase (iNOS) and nuclear factor (NF)-κB (p65) protein expression were evaluated by immunocytochemistry and western blot analysis.</p><p><strong>Results: </strong>Compared with the control, LPS enhanced proinflammatory factor and nitrite concentration in the medium, upregulated iNOS and NF-κB (p65) expressions, and coadministration of ESK decreased proinflammatory cytokine and nitrite levels, and downregulated iNOS and NF-κB (p65) expression. Moreover, ESK exposure enhanced CB2 receptor expression; coadministration of the CB2 receptor antagonist AM630 or the PKC inhibitor chelerythrine (Che), however, markedly blocked the anti-inflammatory effect of ESK in reducing cytokine and nitrite concentration, and downregulating iNOS and NF-κB (p65) expression.</p><p><strong>Conclusions: </strong>These observations demonstrated that the microglial CB2-PKC pathway mediates ESK-induced anti-inflammation in LPS-stimulated microglial cells.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470345/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of risk factors for pulmonary infection in acute ischemic stroke patients following intravenous thrombolysis with alteplase.","authors":"Fei Liu, Jingfei Chen","doi":"10.62347/VZQQ5140","DOIUrl":"https://doi.org/10.62347/VZQQ5140","url":null,"abstract":"<p><strong>Objective: </strong>To identify the risk factors for pulmonary infection in acute ischemic stroke patients treated with intravenous thrombolysis using alteplase.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 110 acute ischemic stroke patients who received intravenous alteplase thrombolysis between January 2019 and November 2022. The patients were categorized into a pulmonary infection group (40 cases) and a non-infection group (70 cases).</p><p><strong>Results: </strong>Multivariate logistic regression analysis identified the following independent risk factors for pulmonary infection: age, National Institutes of Health Stroke Scale (NIHSS) score at admission, underlying lung disease, hypertension, mechanical ventilation, aspiration, confusion, and elevated C-reactive protein (CRP) levels (all P<0.05). The sensitivity and specificity of CRP ifor predicting pulmonary infection were 88.57% and 75.00%, respectively. The NIHSS score demonstrated a sensitivity of 87.14% and a specificity of 70.00%. Further stratification of patients into a good prognosis group (75 cases) and a poor prognosis group (35 cases) revealed that high NIHSS scores at admission, increased fibrinogen (FIB) levels, a thrombolysis window exceeding 3 hours, and concurrent pulmonary infection were independent risk factors for poor prognosis. The area under the ROC curve for NIHSS in predicting prognosis was 0.890, and for FIB, it was 0.854 (P<0.001). The sensitivity and specificity of NIHSS for predicting poor prognosis were 89.33% and 82.86%, respectively, while for FIB, they were 84.00% and 82.86%.</p><p><strong>Conclusions: </strong>These findings indicate that factors such as age, NIHSS score, underlying lung disease, hypertension, and elevated CRP levels significantly contribute to the risk of pulmonary infection in acute ischemic stroke patients. Clinicians should closely monitor these values to manage the risk of pulmonary infection effectively.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11470298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142456118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}