DCUN1D2 is insignificant for spermatogenesis and male fertility in mice.

IF 1.6 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

American journal of translational research Pub Date : 2025-07-25 eCollection Date: 2025-01-01 DOI:10.62347/CLIG5523

Yuxuan Feng, Mingyuan Bao, Wenyi Sheng, Shiqi Liu, Yuchen Cui, Nianchao Zhou, Tiantian Wu, Xiaoyan Huang, Shunyu Hou, Meng Liu

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引用次数: 0

Abstract

Objectives: Defective in cullin neddylation 1 domain containing 2 (DCUN1D2) belongs to the DCNLs family and can induce cell growth arrest and is also related to neddylation. While its potential role in spermatogenesis is hypothesized, the functional significance of DCUN1D2 in male germ cells remains undefined.

Methods: To investigate this, germ cell and Sertoli cell-specific Dcun1d2-knockouts were generated. Sperm parameters were analyzed via computer-assisted sperm analysis (CASA), while histological and immunofluorescence staining were employed to evaluate spermatogenic progression and apoptosis.

Results: Compared with the control group, Dcun1d2 conditional knockout (cKO) mice exhibited no significant differences in histology, semen quality, sperm apoptosis or fertility tests.

Conclusions: This study indicated that DCUN1D2 has no significant effect on mouse spermatogenesis or male fertility. These findings will help avoid redundant studies and provide new information for the study of human fertility genes.

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DCUN1D2对小鼠精子发生和雄性生育能力无显著影响。

目的：cullin neddylation 1 domain containing 2 （DCUN1D2）属于dcnl家族，可诱导细胞生长阻滞，也与neddylation有关。虽然它在精子发生中的潜在作用是假设的，但DCUN1D2在男性生殖细胞中的功能意义仍未明确。方法：制备生殖细胞和支持细胞特异性dcun1d2敲除基因。通过计算机辅助精子分析（CASA）分析精子参数，采用组织学和免疫荧光染色评估精子发生进展和凋亡。结果：与对照组相比，Dcun1d2条件敲除（cKO）小鼠在组织学、精液质量、精子凋亡和生育试验方面均无显著差异。结论：本研究提示DCUN1D2对小鼠精子发生或雄性生育能力无显著影响。这些发现将有助于避免重复研究，并为人类生育基因的研究提供新的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of translational research ONCOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL

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552

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