American journal of translational research最新文献

{"title":"Investigation and analysis of the prevalence of oral diseases and oral health-related quality of life in children with cerebral palsy, and associated factors.","authors":"Yuan Zeng, Jingwen Zhang, Haixia Liu, Xiaoqing Shen","doi":"10.62347/UHOE6541","DOIUrl":"10.62347/UHOE6541","url":null,"abstract":"<p><strong>Objective: </strong>To assess the prevalence of oral diseases and oral health-related quality of life (OHRQoL) in children with cerebral palsy (CP) and identify associated factors.</p><p><strong>Methods: </strong>This retrospective study included 192 children with CP who were admitted to Zhujiang Hospital, Southern Medical University between March 2023 and May 2024. The prevalence of oral diseases, such as dental caries, pulp diseases, malocclusion, and dental trauma, was statistically analyzed. The 5-item Oral Health Impact Profile (OHIP-5) was used to assess OHRQoL. OHIP-5 scores were compared across different degrees of CP severity, and factors influencing OHRQoL were evaluated.</p><p><strong>Results: </strong>Among the 192 children, 50.00% had dental caries, 22.40% had pulp diseases, 16.15% had malocclusion, and 11.46% had dental trauma, with only 14.06% being disease-free. The mean OHIP-5 score was 9.24 ± 1.87. Severe CP was associated with a higher prevalence of oral diseases and higher OHIP-5 scores compared to mild and moderate CP (P < 0.05). Poorer OHRQoL was associated with parental education (vocational school or below), low family income (≤ 5000 RMB/month), infrequent tooth brushing (< 2 times/day), short brushing duration (< 3 minutes), and irregular toothbrush replacement (P < 0.05). Multivariate analysis identified severe CP (OR = 2.050), low family income (OR = 1.923), infrequent brushing (OR = 1.960), short brushing duration (OR = 1.929), and irregular toothbrush replacement (OR = 1.895) as significant risk factors for reduced OHRQoL (P < 0.05).</p><p><strong>Conclusion: </strong>Children with CP have a high prevalence of oral diseases, which significantly impact their OHRQoL. Severe CP and modifiable risk factors, such as oral hygiene practices and socioeconomic status, are critical contributors. Interventions targeting these factors could improve oral health outcomes in this population.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 4","pages":"2773-2781"},"PeriodicalIF":1.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rare esophageal large-cell neuroendocrine carcinoma treated with Serplulimab: a case report.","authors":"Danxia Wen, Xiaoyang Wang, Min Luo, Hengchen Yan","doi":"10.62347/TBQW3054","DOIUrl":"10.62347/TBQW3054","url":null,"abstract":"<p><p>Esophageal large-cell neuroendocrine carcinoma (LCNEC) is a rare and aggressive malignancy that presents diagnostic and therapeutic challenges. We present the case of a 72-year-old female with LCNEC of the distal esophagus who was initially treated with chemotherapy and radiation therapy, followed by immunotherapy due to disease progression. Diagnostic imaging revealed extensive metastases to the abdominal lymph nodes. Histopathological examination confirmed the diagnosis of LCNEC with high-grade squamous intraepithelial neoplasia. The treatment complexities underscore the necessity of a multidisciplinary approach involving medical oncologists, radiation oncologists, and pathologists. While chemotherapy remains the standard, the role of immunotherapies, such as Slurilizumab in LCNEC management is evolving. Subsequent imaging revealed gradual tumor reduction, and the patient was maintained on immunotherapy. The patient has remained on immunotherapy since then, with a progression-free survival of 1 year and 4 months as of the latest follow-up, approximately 3 years post-diagnosis. This case highlights the potential of combining immunotherapy with conventional treatments for disease control. Further research is crucial to optimizing therapeutic strategies and prognostic factors for esophageal LCNEC to enhance clinical outcomes and patient care.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 4","pages":"3247-3251"},"PeriodicalIF":1.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between dynamic-contrast enhanced MRI with histopathological features in atypical HCC using spatial co-registration with biopsy.","authors":"Hans-Jonas Meyer, Johann Potratz, Dörthe Jechorek, Kai Ina Schramm, Jan Borggrefe, Alexey Surov","doi":"10.62347/PJYE7877","DOIUrl":"10.62347/PJYE7877","url":null,"abstract":"<p><strong>Objective: </strong>The relationships between histopathology and imaging remain elusive and investigating the underlying causes of tumor microstructure that result in an imaging phenotype is of clinical importance. In the present study, cross-sectional guided biopsy specimens were used to correlate prebioptic magnetic resonance imaging (MRI) with immunohistochemical staining of histopathologic specimens using precise spatial biopsy localization.</p><p><strong>Methods: </strong>Overall, 52 patients with atypical hepatocellular carcinoma (HCC) were included in the present analysis. All patients were imaged with a 1.5 T clinical scanner at least one month prior to biopsy. The contrast-enhanced dynamic sequences were analyzed with quantified signal intensities. The bioptic specimens were obtained by cross-sectional guided biopsy and was further analyzed for cell density, proliferation index (Ki 67), tumor-infiltrating lymphocytes, tumor-stroma ratio.</p><p><strong>Results: </strong>Per high power field, the mean values of the histologic parameters were as follows: the tumor-stroma ratio was 17.1 ± 20, the cell count was 147.0 ± 60.3, the CD45 count was 7.3 ± 8.0 and the Ki 67-index was 16.9 ± 16.5%. There were no statistically significant correlations between the MRI signal intensities and cell count, tumor-stroma ratio and CD45 count. There was a moderate inverse correlation that was identified between arterial phase signal intensities and Ki 67 max (r=-0.41, P=0.002) and Ki 67 mean (r=-0.37, P=0.005). The signal intensities of the hepatobiliary phase were statistically significantly different between high and low proliferating HCC using thresholds of 20% and 10% (P=0.01 and P=0.02, respectively). The resulting AUC for the 10% threshold was 0.73 and 0.67 for the 20% threshold.</p><p><strong>Conclusion: </strong>DCE-MRI is associated with Ki 67 index in atypical HCC. The hepatobiliary phase could discriminate HCCs according to their Ki 67 index. Quantitative MRI could be used as an imaging-based surrogate for proliferative HCC. Further studies are needed to validate the present results.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 4","pages":"2967-2975"},"PeriodicalIF":1.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MiR-124-3p inhibits proliferation, migration, and epithelial-mesenchymal transformation in gastric cancer by targeting ITGB1.","authors":"Qiuhao Li, Li Zhou, Qiong Liang, Huaxin Qin, Yao Chen, Tiancheng Wang, Qian Wei, Yuanyun Luo, Guanghao Li, Haige Huang","doi":"10.62347/AJPA6532","DOIUrl":"10.62347/AJPA6532","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the functional interaction between miR-124-3p and integrin β1 (ITGB1) in gastric cancer.</p><p><strong>Methods: </strong>A comprehensive approach integrating bioinformatic prediction with experimental validation was employed. The study used dual luciferase reporter assay, CCK-8 assay, RT-qPCR, western blotting, wound healing assay, and transwell assays to systematically investigate the role of the miR-124-3p/ITGB1 regulatory axis in gastric cancer cell models.</p><p><strong>Results: </strong>This study identified a novel regulatory axis involving miR-124-3p and ITGB1. Mechanistic investigations demonstrated that miR-124-3p directly targets ITGB1, as confirmed by dual-luciferase reporter assays. Aberrant expression of miR-124-3p significantly suppressed ITGB1 mRNA and protein levels, leading to impaired oncogenic properties, including reduced proliferation, migration, and invasion of gastric cancer cells. Furthermore, ITGB1 downregulation inhibited gastric cancer cell growth and invasion while suppressing epithelial-mesenchymal transformation (EMT). Notably, key EMT regulators, such as E-cadherin, were up-regulated. These findings suggest that miR-124-3p-mediated ITGB1 downregulation effectively suppresses gastric cancer progression by inhibiting cell proliferation, invasion, and metastasis.</p><p><strong>Conclusions: </strong>miR-124-3p functions as a tumor-suppressive miRNA that inhibits gastric cancer development through targeting ITGB1. The miR-124-3p/ITGB1 axis provides novel insight and may serve as a promising biomarker for gastric cancer diagnosis and treatment.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 4","pages":"2500-2512"},"PeriodicalIF":1.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value of using blood routine indicators combined with artificial intelligence in sepsis patients.","authors":"Jianhui Chen, Minghuan Huang, Rongbin Xu, Yanya Lin, Shijun Chen, Ying Xie, Jianxiong Hu","doi":"10.62347/MMNQ1017","DOIUrl":"10.62347/MMNQ1017","url":null,"abstract":"<p><strong>Objective: </strong>To explore the correlation between Blood Routine Indicators (BRI) and sepsis using machine learning algorithms (MLAs) and evaluate their application in early sepsis for prognosis assessment.</p><p><strong>Methods: </strong>A total of 4,558 blood routine data (BRD) samples were collected, including 149 sepsis patients and 186 patients with common infections (CI). A binary logistic regression model (BLRM) was constructed to predict sepsis based on BRI. Additionally, MLAs were applied, including support vector machines, neural networks, Bayesian classifiers, k-nearest neighbors), decision trees, and random forest classification models (RFCM). The performance of these seven predictive models was evaluated.</p><p><strong>Results: </strong>The RFCM demonstrated the best predictive performance among the MLAs, with accuracy of 86.97%, precision of 87.02%, recall of 86.97%, and F1 score of 0.87. These metrics were significantly higher than those of the BLRM (accuracy: 68.77%, precision PRE: 71.45%, recall: 69.47%, F1 Score: 0.70). In the random forest model, red blood cell volume distribution width (RDW) was identified as the most significant feature, with RDW-coefficient of variation contributing 6.98% and RDW-standard deviation contributing 5.32%.</p><p><strong>Conclusion: </strong>Combining blood routine indicators (BRI) with MLA has considerable potential in predicting sepsis. The RFCM showed the highest predictive value, and RDW may play a crucial role in sepsis prediction.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 4","pages":"2678-2689"},"PeriodicalIF":1.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunotherapy combined with targeted therapy and transcatheter arterial chemoembolization: a promising approach for advanced liver cancer.","authors":"Yuanren Gao, Wenbo Wang, Hongzhe Kang, Yan Liu","doi":"10.62347/KBMZ4400","DOIUrl":"10.62347/KBMZ4400","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical efficacy of combining immunotherapy and targeted therapy with transcatheter arterial chemoembolization (TACE) for advanced liver cancer.</p><p><strong>Methods: </strong>A retrospective analysis was performed on 144 patients with advanced liver cancer, divided into three groups based on treatment choice: TACE group, the TACE + immunotherapy group, and the TACE + immunotherapy + targeted therapy group, with 48 patients in each group. Short-term efficacy, T lymphocyte subsets (CD4+, CD8+, CD4+/CD8+), Th1/Th2 cytokines (interleukin-2 [IL-2], tumor necrosis factor-alpha [TNF-α], IL-4, IL-6), tumor markers (carcinoembryonic antigen, alpha-fetoprotein, carbohydrate antigen 199 [CA199], CA125), angiogenesis-related factors (vascular endothelial growth factor, vascular endothelial growth factor receptor, basic fibroblast growth factor, platelet-derived growth factor), and liver function indicators (alanine aminotransferase, aspartate aminotransferase, total bilirubin, albumin), adverse reactions, and long-term prognosis were compared.</p><p><strong>Results: </strong>Disease control rates for the three groups were 47.92%, 56.25%, and 77.08%, respectively. Objective response rates were 19.00%, 25.00%, and 45.83% (all P < 0.05). The combined therapy group showed significantly improved CD4+, CD8+, CD4+/CD8+, tumor markers, angiogenesis factors, and liver function indicators compared to the other groups (all P < 0.05). Progression-free and cumulative survival rates were also significantly better in the combined therapy group (both P < 0.05).</p><p><strong>Conclusion: </strong>Combining immunotherapy and targeted therapy with TACE offers significant advantages in treating advanced liver cancer, including improved tumor control, enhanced survival, better liver function, reduced tumor marker levels, and enhanced immune response, with a favorable safety profile.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 4","pages":"2790-2799"},"PeriodicalIF":1.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regression analysis and validation of risk factors for upper limb dysfunction following modified radical mastectomy for breast cancer patients.","authors":"Yonggang Li, Shuan Hui","doi":"10.62347/CZYA6232","DOIUrl":"10.62347/CZYA6232","url":null,"abstract":"<p><strong>Objective: </strong>To develop and validate a predictive tool using machine learning models for identifying risk factors for upper limb dysfunction following modified radical mastectomy (MRM) in breast cancer patients.</p><p><strong>Methods: </strong>A total of 768 breast cancer patients who underwent Modified radical mastectomy (MRM) between January 2022 and December 2023 were included in this study. The dataset was divided into a training set (506 cases) and a validation set (262 cases). The collected data encompassed demographic characteristics, clinicopathological features, medical history, and postoperative rehabilitation plans. Predictive analyses were conducted using machine learning models, including support vector machine (SVM), extreme gradient boosting (XGBOOST), Gaussian naïve Bayes (GNB), adaptive boosting (ADABOOST), and random forest. Model evaluation was performed using ten-fold cross-validation, with performance metrics including receiver operating characteristic (ROC) curves, area under the curve (AUC) values, specificity, sensitivity, accuracy, and F1-score. DeLong's test was used to compare AUC values and identify the optimal predictive model.</p><p><strong>Results: </strong>Baseline characteristics showed no significant differences between the training and validation sets (P>0.05). Analysis of factors associated with upper limb dysfunction in the training set revealed significant differences in variables such as age, BMI, cancer type, axillary lymph node dissection, ipsilateral radiotherapy, postoperative rehabilitation plans, and monthly per capita household income (P<0.05). Low correlations were observed among these variables (R values close to 0), indicating minimal multicollinearity. Model performance evaluation showed that the XGBOOST and random forest models demonstrated high AUC values (0.817-0.884) across both the training and validation sets. These models also exhibited superior specificity and sensitivity, indicating strong predictive performance and robustness in identifying patients at risk of postoperative upper limb dysfunction.</p><p><strong>Conclusion: </strong>The XGBOOST and random forest models exhibited excellent predictive accuracy, offering valuable tools for the early identification and personalized management of high-risk patients. These models provide critical data support for postoperative rehabilitation planning and contribute to improving the quality of life for breast cancer patients.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 4","pages":"2614-2628"},"PeriodicalIF":1.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between ultrasound measurements and the expression of Her-2 and CD147 in breast cancer.","authors":"Ping He, Lei Zhang, Fengwei Zhu, Jing Zhao, Yue Liu, Huabin Zhang","doi":"10.62347/JAVK7987","DOIUrl":"10.62347/JAVK7987","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the correlation between ultrasound data and Her-2 and CD147 expressions in breast carcinoma.</p><p><strong>Method: </strong>A retrospective analysis was conducted on 95 patients with invasive breast carcinoma, hospitalized during March 2021 to May 2024, designated as the breast cancer group. Additionally, 80 cases with benign breast lesions were selected as the control group. Contrast-enhanced ultrasound (CEUS) was performed on both groups. Immunohistochemistry was used to detect the expression of Her-2 and CD147 proteins in breast cancer and adjacent tissues, while RT-qPCR was employed to measure the mRNA expression of Her-2 and CD147 in the cancer and adjacent tissues.</p><p><strong>Results: </strong>The peak time in the breast cancer group was shorter than in the control group (<i>P</i><0.05). Conversely, the enhancement amplitude, slope of ascending branch, area under the curve, and gradient were higher in the breast cancer group than in the control group (all <i>P</i><0.05). There was a significant negative correlation between peak time of ultrasound parameters and the relative expression of Her-2 and CD147 mRNA (<i>P</i><0.05). In contrast, the enhancement amplitude, slope of the ascending branch, area under the curve, and gradient exhibited positive correlations with the relative expression of Her-2 and CD147 mRNA in cancer tissues (<i>P</i><0.05).</p><p><strong>Conclusion: </strong>The contrast-enhanced ultrasound data of malignant breast tumors significantly differed from those of benign tumors, demonstrating good diagnostic value for breast cancer. In addition, these ultrasound data were significantly correlated with the expression of Her-2 and CD147 in tumor tissues, providing valuable insight for the non-invasive assessment of early-stage breast cancer and offering a reference for diagnosis and treatment planning.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 4","pages":"3109-3118"},"PeriodicalIF":1.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness and safety profiles of percutaneous nephrolithotomy and ureteroscopic lithotripsy for proximal ureteral calculi.","authors":"Hong Jiang, Banggao Huang, Hongjun Lu, Shaojiang Li","doi":"10.62347/GYUQ6118","DOIUrl":"10.62347/GYUQ6118","url":null,"abstract":"<p><strong>Objective: </strong>To compare the effectiveness and safety of percutaneous nephrolithotripsy (PCNL) and ureteroscopic lithotripsy (URSL) in treating proximal ureteral stones.</p><p><strong>Methods: </strong>The clinical data of 86 patients with proximal ureteral stones, admitted to the Department of Urology at the First People's Hospital of Chun'an County from May 2021 to May 2023, were retrospectively analyzed. Patients were divided into a PCNL group (n=41) and a URSL group (n=45) based on the treatment modality. Key outcomes compared between the two groups included surgical time, intraoperative blood loss, incidence of complications, hospital stay, stone clearance rate, lithotripsy success rate, renal function indicators, and levels of inflammatory factors.</p><p><strong>Results: </strong>The PCNL group had significantly higher intraoperative blood loss and longer postoperative hospital stay compared with the URSL group, whereas the operative time was remarkably shorter (<i>P</i><0.05). The stone clearance and lithotripsy success rates were considerably higher in the PCNL group than in the URSL group, and the complication rates were significantly lower (<i>P</i><0.05). After treatment, the levels of renal function indicators, including serum creatinine (Scr), blood urea nitrogen (BUN), and cystatin C (CysC), decreased significantly in both groups, with the PCNL group showing more pronounced decrease compared to the URSL group (<i>P</i><0.05), approaching normal levels. Additionally, while the levels of procalcitonin (PCT), interleukin-13 (IL-13), and high-sensitivity C-reactive protein (hs-CRP) were elevated in both groups after treatment, the PCNL group showed significantly lower levels of these inflammatory markers compared to the URSL group (all <i>P</i><0.05).</p><p><strong>Conclusion: </strong>Both PCNL and URSL are effective treatments for proximal ureteral stones. However, PCNL offers superior clinical effectiveness and safety.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 4","pages":"2984-2991"},"PeriodicalIF":1.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of stellate ganglion block on inflammation and autophagy of spinal cord neurons in rats with neuropathic pain after spinal cord injury.","authors":"Yuanxin Huang, Hai Xu, Dongmei Mao, Jinqi Shao, Lili Deng, Tingyu Wang, Zeqing Liao, Xue Li, Yutao Chen, Jing Yao, Zhongjie Zhang","doi":"10.62347/QEVD3665","DOIUrl":"10.62347/QEVD3665","url":null,"abstract":"<p><strong>Objective: </strong>To assess the therapeutic effects of stellate ganglion block (SGB) on spinal cord injury (SCI)-induced neuropathic pain in rats, and to explore its potential mechanisms in alleviating neuropathic pain, thereby providing a theoretical foundation for clinical treatment.</p><p><strong>Methods: </strong>A rat model of SCI was established, and animals were randomly assigned to one of three groups: the sham surgery group (Sham), the SCI group (SCI), or the SCI group treated with SGB (SCI + SGB). Motor function was assessed using the Basso Beattie Bresnahan (BBB) locomotor rating scale, while thermal hyperalgesia was evaluated using hot plate test. Enzyme-linked immunosorbent assay (ELISA) was utilized to measure the levels of inflammatory cytokines, including interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α), within the spinal cord. Hematoxylin-eosin (HE) staining was performed to observe spinal cord histopathology. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining was used to detect apoptotic cells, and transmission electron microscopy was employed to visualize autophagosomes. Expression of autophagy-related proteins LC3-II/LC3-I and p62 was examined via Western blotting.</p><p><strong>Results: </strong>Compared with the sham group, rats in the SCI group displayed impaired hind limb motor function, decreased pain thresholds, elevated inflammatory cytokine levels, significant spinal cord pathology, increased apoptosis, altered expression of autophagy-related protein, and disrupted autophagic flux. In contrast, SGB treatment improved motor function, alleviated pain, reduced inflammatory cytokines levels, mitigated spinal cord injury and apoptosis, and enhanced autophagy with improved autophagic flux.</p><p><strong>Conclusions: </strong>Stellate ganglion block alleviates neuropathic pain in SCI-induced rats by reducing pro-inflammatory cytokine levels, mitigating spinal cord apoptosis and injury, promoting autophagy, and restoring autophagic flux in the spinal cord.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 4","pages":"3063-3073"},"PeriodicalIF":1.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}