American journal of translational research最新文献

{"title":"Cadherin family genes in non-small cell lung cancer: implications for diagnosis, prognosis, and targeted therapy.","authors":"Yirui Wang, Xuan Qin, Wei Dong, Changjiang Lei, Su Zheng, Mohamed M Salem, Mounir M Bekhit, Nihal Almuraikhi","doi":"10.62347/SDZI3679","DOIUrl":"10.62347/SDZI3679","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed to explore the diagnostic, prognostic, and therapeutic values of cadherin family genes (CDH1, CDH2, and CDH3) in non-small cell lung cancer (NSCLC) subtypes: lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC).</p><p><strong>Methodology: </strong>We analyzed the expression of CDH1, CDH2, and CDH3 in LUAD and LUSC using TCGA and TIMER2 data, and evaluated protein levels through immunostaining data from the HPA database. Gene expression across LUAD and LUSC stages was examined using GEPIA2. Methylation and mutation analyses were conducted vby OncoDB and cBioPortal, respectively. Prognostic significance was assessed through survival analyses using the KM Plotter tool. Gene enrichment and immune infiltration correlations were investigated using DAVID and GSCA databases. Knockdown experiments in PC9 cells were performed to assess the effects of CDH1 and CDH2 on cell proliferation, colony formation, and wound healing.</p><p><strong>Results: </strong>The expression of CDH1, CDH2, and CDH3 was significantly elevated in both LUAD and LUSC. Methylation analysis revealed reduced promoter methylation of cadherin genes in tumor samples compared to normal tissues. Mutational analysis showed that CDH2 exhibited the highest mutation frequency (63%), followed by CDH3 (23%) and CDH1 (19%). Survival analysis indicated that higher expression of CDH1, CDH2, and CDH3 was associated with poor prognosis in both LUAD and LUSC. Knockdown of CDH1 and CDH2 in PC9 cells resulted in reduced cell proliferation, colony formation, and impaired wound healing, with CDH2 knockdown showing more pronounced effects.</p><p><strong>Conclusion: </strong>CDH1, CDH2, and CDH3 were upregulated in LUAD and LUSC, contributing to tumor progression and poor prognosis. Knockdown of CDH1 and CDH2 in PC9 cells impaired proliferation, colony formation, and wound healing, highlighting their potential as therapeutic targets.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 5","pages":"3718-3737"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cystic fibrosis caused by homozygous <i>CFTR</i> gene mutation leading to pulmonary involvement: a case report.","authors":"Ji-Peng Duan, Ru-Fen Dai, Wan-Hong Ma, Tian-Shu Luo, Zhi-Zhen Duan, Xue-Fen Zhang, Yan-Ling Zhang, Ming-Wei Liu","doi":"10.62347/ENOM2926","DOIUrl":"10.62347/ENOM2926","url":null,"abstract":"<p><p>Cystic fibrosis (CF) is an autosomal recessive monogenic disorder caused by mutations in the CF transmembrane conductance regulator (<i>CFTR</i>) gene, resulting in impaired <i>CFTR</i> protein function. Predominantly affecting Caucasians, CF involves multiple organ systems, including the lungs, pancreas, liver, gastrointestinal tract, and reproductive system. In contrast, CF remains rare among Asian populations, particularly within the Chinese demographic. Reported cases in China predominantly feature heterozygous <i>CFTR</i> mutations, with no confirmed instances of homozygous mutations. A 15-year-old male presented with a 6-year history of recurrent cough and purulent yellow-green sputum production, without hemoptysis. Whole exome sequencing identified a homozygous <i>CFTR</i> mutation, NM_000492.4:c.2290C>T (p.Arg764*), confirming the diagnosis of CF complicated by pulmonary infection. The patient received intravenous cefoperazone/sulbactam (2.25 g every 12 hours) and moxifloxacin (400 mg once daily). Symptomatic improvement was achieved after 2 weeks, and azithromycin was prescribed (three times weekly) upon discharge. This case highlights the importance of considering <i>CFTR</i> gene mutations in patients with prolonged respiratory symptoms (recurrent cough and sputum production) and imaging findings indicative of pulmonary CF. Whole exome sequencing is recommended to determine the genetic etiology in such cases and guide targeted management.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 5","pages":"3813-3823"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in macrophage immune regulation of atherosclerosis.","authors":"Shuangyou Deng, Yanjuan Liu, Ying Wang, Shumeng Zhang, Xing Chen, Zixuan Yu, Lingli Chen, Jie Li","doi":"10.62347/GMTC2479","DOIUrl":"10.62347/GMTC2479","url":null,"abstract":"<p><p>Atherosclerosis is a chronic inflammatory disease that contributes to cardiovascular conditions, including coronary artery disease and stroke. Macrophages are central to its pathogenesis, accumulating in arterial walls, engulfing oxidized low-density lipoprotein (oxLDL), and forming foam cells that exacerbate inflammation. These macrophages can polarize into two main subsets: M1 macrophages, which promote inflammation, and M2 macrophages, which resolve inflammation and support tissue repair. The balance between these subsets is crucial for plaque progression and stability. Recent studies have elucidated the immune regulatory functions of macrophages in modulating atherosclerotic plaque formation and vulnerability. Understanding the mechanisms governing macrophage activation, polarization, and immune interactions presents promising therapeutic targets aimed at stabilizing plaques and preventing cardiovascular events. This review summarizes current research on the role of macrophages in atherosclerosis and discusses potential therapies targeting macrophage immune regulation.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 5","pages":"3261-3275"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Twenty years of misdiagnosis of X-linked adrenoleukodystrophy: a case report.","authors":"Deyu Xia, Xiaochen Fu, Yiran Meng, Chunjing Yang, Wei Wang, Zhongkui Wang","doi":"10.62347/MGJG8532","DOIUrl":"10.62347/MGJG8532","url":null,"abstract":"<p><p>X-linked adrenoleukodystrophies (x-ALDs) constitute a group of rare neurological disorders characterized by both genetic and clinical heterogeneity. The diagnostic process necessitates detecting elevated very long-chain fatty acid concentrations in conjunction with genetic analysis of the ATP-binding cassette transporter D1 (ABCD1) gene. This report presents a case of an atypical manifestation and clinical progression of x-ALD, which was initially misdiagnosed. A 38-year-old male patient with x-ALD exhibited progressively worsening gait disturbances and lower limb weakness. Over two decades of medical intervention, the patient had persistently been diagnosed and treated for hereditary spastic paraplegia. A novel hemizygous mutation in exon 1 (c.356dupC) of the ABCD1 gene was identified. The patient's diagnosis was subsequently revised to x-ALD. This case highlights the necessity of considering x-ALD as a potential differential diagnosis in patients presenting with gradually progressive spastic paraplegia.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 5","pages":"3571-3574"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study on the clinical efficacy and safety of conventional open thyroidectomy and bilateral areolar approach thyroidectomy.","authors":"Qinghua Wu, Chunyu Chai, Xiting Bao, Songyao Jiang, Xin Liu","doi":"10.62347/IJSQ9396","DOIUrl":"10.62347/IJSQ9396","url":null,"abstract":"<p><strong>Objective: </strong>To compare the clinical outcomes and safety profiles of conventional open thyroidectomy and bilateral areolar approach thyroidectomy.</p><p><strong>Methods: </strong>A total of 72 patients who underwent endoscopic thyroidectomy at Ruijin Hospital, Shanghai Jiao Tong University School of Medicine between August 2021 and March 2024 were retrospectively included as the endoscopic group (EG). Sixty-five patients who underwent conventional open thyroidectomy during the same period served as the control group (CG). Baseline characteristics, intraoperative parameters, postoperative outcomes, pain levels, incidence of postoperative complications, and three-month Numerical Scoring System (NSS) aesthetic scores were compared between the two groups.</p><p><strong>Results: </strong>Compared to the CG, the EG had a longer operative time but experienced less intraoperative blood loss and a shorter hospital stay (both P < 0.05). Postoperative white blood cell counts and C-reactive protein levels were significantly lower in the EG (both P < 0.05). Visual Analogue Scale scores on postoperative days 1, 2, and 3 were also significantly lower in the EG (all P < 0.05). The incidence of complications was lower in the EG than in the CG (P < 0.05). At the three-month follow-up, the EG demonstrated significantly higher NSS aesthetic scores compared to the CG (P < 0.05). Multivariable analysis identified age ≥ 50 years, open surgery, operative time ≥ 130 minutes, and intraoperative blood loss ≥ 60 mL as independent risk factors for postoperative complications (all P < 0.05).</p><p><strong>Conclusion: </strong>Bilateral areolar approach thyroidectomy offers advantages over conventional open thyroidectomy, including reduced tissue trauma, faster postoperative recovery, lower pain levels, a reduced incidence of complications, and superior short-term aesthetic satisfaction.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 5","pages":"3908-3916"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170384/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical study on ultrasound-guided percutaneous microwave ablation for hepatocellular carcinoma.","authors":"Rongrong Li, Yang Yu","doi":"10.62347/ESHV1093","DOIUrl":"10.62347/ESHV1093","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the efficacy, safety, and survival benefits of ultrasound-guided microwave ablation (MWA) compared to surgical resection (SR) in patients with hepatocellular carcinoma (HCC).</p><p><strong>Methods: </strong>This retrospective study included 100 patients with HCC who underwent either ultrasound-guided MWA (n = 58) or SR (n = 42). Baseline characteristics, liver function tests, tumor markers, complications, and survival outcomes were analyzed. Tumor response was assessed using Response Evaluation Criteria in Solid Tumors criteria, with follow-up evaluations at 12, 24, and 36 months.</p><p><strong>Results: </strong>MWA demonstrated superior clinical outcomes compared to SR (P < 0.001). Post-treatment levels of liver enzymes (alanine aminotransferase, aspartate aminotransferase) and total bilirubin. Both groups showed significant reductions in alpha-fetoprotein and carbohydrate antigen 19-9 levels, with no significant difference between them (both P < 0.001). The complication rate was significantly lower in the MWA group (P < 0.001). Tumor response, including complete response (CR) and overall response rate (ORR), was higher in the MWA group (CR: 40 vs. 20; ORR: 86.2% vs. 65.6%). Additionally, progression-free survival (PFS) and overall survival (OS) at 12, 24, and 36 months were significantly better in the MWA group (all P < 0.001).</p><p><strong>Conclusion: </strong>Ultrasound-guided MWA provides notable advantages over SR in the treatment of HCC, including less hepatic injury, fewer complications, and improved PFS and OS. These findings support MWA as a safe, minimally invasive, and effective alternative for HCC management.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 5","pages":"3640-3648"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of PECS II versus RISS block for postoperative analgesia in breast cancer surgery: a randomized controlled trial.","authors":"Xiaoyu Zhang, Yulong Jia, Chenxu Chou, Xiaguang Duan","doi":"10.62347/CEED1530","DOIUrl":"10.62347/CEED1530","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the analgesic effects of Pectoral Nerve Block Type II (PECS II) versus Rhomboid Intercostal and Subserratus Plane Block (RISS) after modified radical mastectomy (MRM) for breast cancer.</p><p><strong>Methods: </strong>This prospective, double-blind, randomized controlled trial recruited 67 female patients undergoing unilateral MRM from December 1, 2023, to December 1, 2024 at Inner Mongolia Baogang Hospital. Patients were randomly assigned (1:1) to the PECS II group (n=30) or the RISS group (n=30). Primary outcomes included Quality of Recovery (QoR-40) scores at 6 and 24 hours and Visual Analogue Scale (VAS) pain scores in the PACU and at 2, 6, 12, 24, and 48 hours postoperatively. Secondary outcomes included sufentanil consumption via PCIA, the number of effective compressions, remifentanil use during surgery, additional analgesic administrations, sleep quality, and adverse events.</p><p><strong>Results: </strong>The PECS II group demonstrated significantly lower VAS scores than the RISS group at rest and during movement in the PACU and at 2, 6, 12, and 24 hours (P<0.05). The QoR-40 score at 6 hours was significantly higher in the PECS II group (P<0.0001). The remifentanil consumption, sufentanil dosage and effective compressions via PCIA during surgery were also significantly lower in the PECS II group, compared to the RISS group (P<0.0001).</p><p><strong>Conclusion: </strong>PECS II block appears to be a more effective analgesic technique than RISS block for patients undergoing breast cancer surgery, providing better pain control, reducing opioid consumption, and potentially facilitating faster recovery.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 5","pages":"3971-3981"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dectin-1 participates in β-glucan- or PMA-induced neutrophil extracellular trap formation during antifungal defense.","authors":"Shoude Zhang, Ying Lu, Yuan Zhao, Zhanwei Dong, Mao Jin, Mina Xu, Hong Pan, Mang Xiao","doi":"10.62347/YQCM4496","DOIUrl":"10.62347/YQCM4496","url":null,"abstract":"<p><strong>Objectives: </strong>The formation of neutrophil extracellular traps (NETs) plays a crucial role in neutrophil-mediated defense against fungal infections and has become a hot topic of immunological research. This study aimed to investigate whether high expression of Dectin-1, a key pattern recognition receptor, contributes to NET formation in response to fungal pathogens.</p><p><strong>Methods: </strong>Human neutrophils were isolated and characterized, then stimulated with cell wall β-glucan to induce NET formation. Phorbol 12-myristate 13-acetate (PMA), a diacylglycerol mimetic, was used as a positive control. Dectin-1 antibody was used to determine the functional significance of Dectin-1 in the formation of NETs. NET formation was detected by Sytox Green staining, myeloperoxidase (MPO) and neutrophil elastase (NE) immunofluorescence staining, and western blot analysis. The relative kits, 2',7'-dichlorodihydrofluorescein diacetate staining and MitoSOX Red staining were used to determine the mechanism of Dectin-1 induced NET formation.</p><p><strong>Results: </strong>Dectin-1 was overexpressed in β-glucan- and PMA-treated neutrophils. Dectin-1 deficiency reduced NET formation, accompanied by decreased Sytox Green fluorescence, lower levels of dsDNA content, and decreased expression of NE, MPO and citrullinated histone H3 (H3Cit). Dectin-1-mediated NET formation was dependent on reactive oxygen species (ROS) produced by NADPH oxidase (NOX), NOX2 protein and mitochondrial superoxide. Moreover, up-regulated Dectin-1 expression activated the extracellular regulated protein kinases (ERK) and p38 MAPK pathways, which were critical for the induction of NETs.</p><p><strong>Conclusion: </strong>Dectin-1 promotes NET formation in neutrophils stimulated by β-glucan or PMA through activation of the ERK and p38 signaling pathways, which might contribute to defense against fungal pathogens.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 5","pages":"3898-3907"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogen distribution and risk factors of positive stone culture for patients with upper urinary tract stone.","authors":"Yu Cao, Hui Cao","doi":"10.62347/GSAT9658","DOIUrl":"10.62347/GSAT9658","url":null,"abstract":"<p><strong>Background: </strong>Upper urinary tract stones are often complicated by perioperative infections. Bacterial culture analysis of calculi can reveal pathogen distribution on stone surfaces and help identify risk factors for positive culture outcomes, aiding in infection control.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 173 patients with upper urinary tract stones who underwent holmium laser lithotripsy. Clinical characteristics potentially influencing positive stone cultures were collected and used to establish a binary logistic regression model for identifying risk factors. Additionally, the correlation between positive stone cultures and postoperative infection markers was analyzed.</p><p><strong>Results: </strong>Among the 173 patients, 44 had positive stone cultures, and 47 pathogen strains were detected. Forty-six patients had positive urine cultures, with 49 pathogen strains identified. The main pathogens in stone cultures were <i>Escherichia coli</i> (22 strains, 46.81%), <i>Proteus mirabilis</i> (5 strains, 10.64%), <i>Pseudomonas aeruginosa</i> (4 strains, 8.51%), and <i>Klebsiella pneumoniae</i> (3 strains, 6.38%). In urine culture, the main pathogens were <i>Escherichia coli</i> (26 strains, 53.06%), <i>Klebsiella pneumoniae</i> (5 strains, 10.2%), and <i>Acinetobacter baumannii</i> (4 strains, 8.16%). Binary logistic regression identified the following risk factors for positive stone cultures: positive urine white blood cells (OR = 2.881, 95% CI = 1.070-7.760, P = 0.036), hydronephrosis (OR = 5.644, 95% CI = 2.168-14.696, P < 0.0001), struvite stones (OR = 7.512, 95% CI = 1.864-30.283, P = 0.005), and a history of diabetes (OR = 6.580, 95% CI = 1.820-23.791, P = 0.004). Spearman correlation analysis showed a strong correlation between positive stone cultures and postoperative fever (r = 0.666) and CRP failure to return to normal 48 hours post-surgery (r = 0.633), both of which were higher than the correlation with urine bacterial culture.</p><p><strong>Conclusion: </strong><i>Escherichia coli</i> is the predominant pathogen in stone cultures. Risk factors for positive cultures include positive urine leukocytes, hydronephrosis, struvite stones, and a history of diabetes. Positive stone cultures are associated with a higher risk of postoperative infection compared to positive urine cultures.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 5","pages":"3658-3664"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture improves lipid metabolism via proteome and gut microbiota profiling in obese rats.","authors":"Xia Liu, Chang She, Xiang Li, Menglin Yang, Yunhui Zhang, Penglong Yu, Yanqiu Tong, Xunhao Zhang, Xiurong Tian","doi":"10.62347/ZQZS9458","DOIUrl":"10.62347/ZQZS9458","url":null,"abstract":"<p><strong>Objectives: </strong>Electroacupuncture (EA) is widely utilized for obesity treatment, but the mechanisms remain incompletely understood. This study explored electroacupuncture effects on gut microbiota and adipose tissue proteomics in obese rats.</p><p><strong>Methods: </strong>Twenty-two Sprague-Dawley male rats were randomly divided into the control (n=6) and model (n=16) groups. After establishing an obesity model, the rats were further categorized into the high-fat diet (n=6) and EA groups (n=6). The EA group underwent EA at \"Quchi\" (LI 11) and \"Zusanli\" (ST 36) acupoints for 21 days. Body weight was measured on alternate days, and hematoxylin and eosin staining were used to assess pathological changes in the adipose tissue and liver. Serum lipid levels were measured using a biochemical analyzer. Amplicon sequencing for bacterial 16S rRNA gene and liquid chromatography-mass spectrometry determined the gut microbiota structure and short-chain fatty acid (SCFA) content in the fecal sample, respectively. Tandem mass tag quantitative proteomics was used to analyze differentially expressed adipose tissue proteins.</p><p><strong>Results: </strong>EA significantly reduced body weight, improved adipose tissue pathology, and decreased total cholesterol and triglyceride levels. EA significantly modulated key gut microbiota involved in lipid metabolism and increased SCFA content, particularly acetic and propionic acids. The proteomic analysis revealed EA-mediated protein regulation, including Hmgcs1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1) and Fabp3 (Fatty Acid-Binding Protein 3), associated with peroxisome proliferator-activated receptor (PPAR) signaling pathway regulation.</p><p><strong>Conclusion: </strong>EA improved dysregulated gut microbiota and SCFAs, modulated PPAR signaling, and improved lipid metabolism; thus, it has a potential role in obesity treatment.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 5","pages":"4008-4022"},"PeriodicalIF":1.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12170374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}