American journal of translational research最新文献

{"title":"New insights in nanocrystal technology chemotherapeutic drugs targeting cancer with a translational research paradigm.","authors":"Furqan Muhammad Iqbal, Florence Delie, Muhammad Omer Iqbal, Safia Gul, Sumaira Kanwal, Muhammad Muzammal, Aftab Ahmad, Nayla Munawar","doi":"10.62347/ETMK5291","DOIUrl":"10.62347/ETMK5291","url":null,"abstract":"<p><p>Nanocrystal formulation has been increasingly investigated for overcoming the limitations of Biopharmaceutical Classification System (BCS) Class II and IV chemotherapeutic agents. Nanocrystal (NC) formation is widely accepted for increasing the solubility, biological barrier permeability, and cell internalization of poorly-soluble chemotherapeutic drugs. Nanocrystalization improves the bioavailability of anticancer agents, increasing their cytotoxicity and effectiveness for cancer treatment. NCs are nanodrug particles that are coated with a thin polymer or surfactant layer, that enhance their stability, solubility, and internalization in tumor cells. For active targeting, NCs can be decorated with ligands, e.g., proteins and amino acids. NCs also undergo passive targeting by high cellular uptake and retention in the mononuclear phagocyte system (MPS). They are prepared by either top-down, or bottom-up methods or a combination and can be scaled up for industrial manufacturing. NCs are safely administered by oral, parenteral, or transdermal routes. This review highlights the role of morphology and stabilizer, with brief discussions on nanocrystal production, ligand conjugation on drug nanocrystal targeting, and uptake in cancer cells. The benefits of NC formulations over conventional drug delivery are presented by discussing aspects of cytotoxicity studies of anticancer drugs.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"5829-5847"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for comorbid hypertension in elderly patients with type 2 diabetes.","authors":"Wei-Xian Zheng, Ping Liao, Li-Li Luo, Jin Gu, Lian Du, Yi-Zhu Tian","doi":"10.62347/UCOV1190","DOIUrl":"10.62347/UCOV1190","url":null,"abstract":"<p><strong>Objective: </strong>To identify risk factors for hypertension (HT) in elderly patients with diabetes, using retrospective data analysis.</p><p><strong>Methods: </strong>A total of 139 elderly patients with diabetes were recruited, including 61 in the HT group and 78 to the non-HT group. Demographic characteristics, lifestyle factors, and serum biochemical marker were compared between the two groups. Binary logistic regression was performed to identify independent risk factors for HT, and their predictive utility was later examined using receiver operating characteristic (ROC) analysis. Cardio-cerebrovascular events were recorded during a two-year follow-up period, along with scores from the 36-item Short-Form Health Survey Questionnaire (SF-36).</p><p><strong>Results: </strong>Compared with the non-HT group, patients with HT were significantly older and had longer disease duration, higher low-density lipoprotein cholesterol (LDL-C), and a higher incidence of cardio-cerebrovascular events. Besides, patients with HT were more likely to smoke, consume excessive alcohol, exercise less, and had lower high-density lipoprotein cholesterol (HDL-C) levels and SF-36 scores. According to binary logistic regression-based analysis, disease duration, alcohol consumption, exercise frequency, HDL-C, LDL-C, UA, and microalbuminuria were significant contributors to HT in elderly people suffering from diabetes. Individually, these factors yielded area under the curve (AUC) values ranging from 0.580 to 0.740, while their combined model achieved an AUC of 0.910.</p><p><strong>Conclusions: </strong>Prolonged disease duration, heavy alcohol consumption, physical inactivity, decreased HDL-C, and elevated LDL-C, UA, and microalbuminuria are significant risk factors for HT in elderly patients with diabetes. A combined assessment of these variables provides strong predictive value for identifying HT risk.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6453-6461"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemotherapy-induced suppression of colorectal cancer-associated gut microbiota and modulation of host miRNA expression.","authors":"Chunli Wang, Baoyang Kuang, Kuan Yang, Xuewen Xiao, Caixia Wu, An Li, Xiaoli Zeng, Xiangcai Wang, Jianming Ye","doi":"10.62347/XXCS3377","DOIUrl":"10.62347/XXCS3377","url":null,"abstract":"<p><strong>Objectives: </strong>To characterize gut microbiome alterations in colorectal cancer (CRC) patients following cancer chemotherapy (CCT) and to explore associations with bacterial translocation and host miRNA dynamics.</p><p><strong>Methods: </strong>Stool samples were prospectively collected from 20 CRC patients who had undergone radical surgery followed by adjuvant chemotherapy (CAPOX/mFOLFOX6). Stool samples were collected pre- and post-CCT. Microbial profiling was performed using 16S rRNA sequencing. Bacterial translocation was assessed by measuring serum anti-Lipopolysaccharides (LPS) IgA/IgG levels by ELISA. miRNA expression of miR-143 and miR-145 was quantified using qPCR.</p><p><strong>Results: </strong>Post-CCT samples showed significant increases in gut microbiome diversity (<i>P</i><0.05), with higher relative abundances of <i>Porphyromonas, Peptostreptococcus,</i> and <i>Parvimonas</i>, and decreased abundances of <i>Faecalibacterium</i> and <i>Ruminococcaceae</i> (P<0.005). Network analysis identified <i>Peptostreptococcus</i> and <i>Parvimonas</i> as possible CRC-associated taxa. Serum anti-LPS IgA and IgG levels significantly declined post-CCT, indicating reduced bacterial translocation. Concurrently, miR-143 and miR-145 levels increased more than twofold post-CCT (P<0.01), positively correlating with microbial shifts.</p><p><strong>Conclusion: </strong>CCT induces significant remodeling of CRC-associated gut microbiota, characterized by suppression of pathogenic genera and enrichment of pro-inflammatory taxa. These changes align with reduced bacterial translocation and increased expression of tumor-suppressive miRNAs, suggesting that CCT exerts dual therapeutic effects by simultaneously modulating microbial communities and host molecular pathways.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6573-6586"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effect of permeable resin and composite resin filling combined with self-etching adhesive in the treatment of dental caries in children.","authors":"Chen Ma, Xiaofei Wang, Jin Li","doi":"10.62347/VPDH5139","DOIUrl":"10.62347/VPDH5139","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the clinical effect of permeable resin filling compared with composite resin filling compared with a self-etching adhesive in the treatment of dental caries in children.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on the clinical data of 118 children with dental caries treated at Shenzhen Second People's Hospital from April 2021 to April 2024. According to the treatment plans, patients were divided into two groups: the control group (n = 59), treated with composite resin filling and a self-etching adhesive, and the observation group (n = 59), treated with permeable resin filling. The overall clinical efficacy and adverse reactions were assessed at 3, 6 and 12 months after treatment. In addition, inflammatory markers [Tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8)], periodontal health indicators [bleeding index (BI), plaque index (PLI), gingival index (GI), probe depth (PD)], restoration quality (marginal adaptation rate and retention rate), tooth sensitivity, treatment cooperation [visual analog scale (VAS) score, Tactile value, Schiff score, Houpt score, Frank1 score] and oral health-related quality of life-based on the Children's Oral Health Impact Profile (COHIP) scale were evaluated before and 3 months after treatment.</p><p><strong>Results: </strong>At 3, 6 and 12 months after treatment, the observation group showed significantly higher overall clinical efficacy compared to the control group (P < 0.05). Subgroup analysis revealed that among patients with superficial caries, the observation group achieved a significantly higher response rate (P < 0.05). After treatment, levels of TNF-α, IL-6, IL-8, BI, PLI, GI, PD, VAS, Schiff score, Houpt score, Frank1 score, and COHIP score decreased in both groups, while tactile value increased. Moreover, the observation group exhibited greater improvements across all inflammatory, periodontal, restorative, tooth sensory, behavioral and quality of life metrics compared to the control group (P < 0.05). The incidence of adverse reactions was 5.08% in the observation group and 13.56% in the control group, with no statistically significant difference (P > 0.05).</p><p><strong>Conclusion: </strong>Permeable resin filling demonstrates superior permeability, enhanced caries repair outcomes, and favorable safety in the treatment of pediatric dental caries, making it a clinically valuable therapeutic option.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6601-6609"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FBXO22 regulates proliferation, migration, and invasion of esophageal cancer cells via the WNT/β-catenin signaling pathway.","authors":"Rui Zheng, Wangwang Xu, Li Ma, Jing Wang, Jia Ma, Chen Liu, Li Yu, Yuyun Li, Yingjie Zhang, Hui Xu","doi":"10.62347/TOAI4814","DOIUrl":"10.62347/TOAI4814","url":null,"abstract":"<p><strong>Objectives: </strong>FBXO22, a member of the F-box family, plays a crucial role in cancer development and progression. However, its expression and biological functions in esophageal cancer (ESCA) remain poorly understood.</p><p><strong>Methods: </strong>In this study, we investigated FBXO22 expression in ESCA cancer tissues using immunohistochemistry and Western blot analyses. Functional assays, including CCK-8, flow cytometry, Western blot, scratch healing, and Transwell migration and invasion assays, were employed to evaluate the effects of FBXO22 modulation on ESCA cell viability, apoptosis, migration, and invasion. Additionally, a nude mouse model was used to assess the impact of FBXO22 silencing on tumor growth.</p><p><strong>Results: </strong>We found that FBXO22 expression was upregulated in ESCA tissues compared to normal tissues. Silencing FBXO22 inhibited ESCA cell viability, migration and invasion while promoting apoptosis. Conversely, FBXO22 overexpression had the opposite effects. Mechanistically, FBXO22 was found to influence the WNT/β-catenin signaling pathway, and its silencing retarded tumor growth in vivo.</p><p><strong>Conclusions: </strong>Our findings highlight the critical role of FBXO22 in ESCA progression and suggest it as a potential therapeutic target for ESCA.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"5962-5974"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Albumin ratio and liver-to-abdominal area ratio are promising prognostic indicators for patients with post-hepatitis B cirrhosis.","authors":"Xin Luo, Jichang Li, Yi'an Ma, Lingling Yang, Naqi Ma","doi":"10.62347/KKMB6772","DOIUrl":"10.62347/KKMB6772","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the clinical relevance of the Albumin Ratio (AR) and Liver-to-Abdominal Area Ratio (LAAR) as prognostic markers for patients with hepatitis B-related cirrhosis.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted on 278 patients diagnosed with hepatitis B-associated cirrhosis at Baoji Central Hospital from January 2017 to December 2020. Clinical data, laboratory results (AR), and imaging data (LAAR) were collected. Prognostic value of AR and LAAR was assessed using Kaplan-Meier survival analysis, Cox proportional hazards regression, and receiver operating characteristic (ROC) curves. A nomogram integrating multiple variables was developed and validated to dynamically predict 6-, 12-, and 24-month survival outcomes.</p><p><strong>Results: </strong>Both AR and LAAR were significantly reduced in the deceased group compared to survivors (P<0.05). Cox regression analysis identified AR (HR=1.86, 95% CI: 1.40-2.45, P<0.001) and LAAR (HR=1.67, 95% CI: 1.25-2.22, P=0.002) as independent prognostic factors. Kaplan-Meier survival curves revealed significantly shorter survival in patients with lower AR and LAAR (P<0.001). Time-dependent ROC analysis indicated good predictive performance for AR (AUC up to 0.85), while LAAR showed relatively low discrimination, with AUCs ranging from 0.507 to 0.623. The nomogram, incorporating both AR and LAAR, exhibited excellent discrimination (C-index =0.950), and its predictive accuracy was validated through calibration curves.</p><p><strong>Conclusion: </strong>AR and LAAR are critical prognostic indicators in hepatitis B-related cirrhosis. The nomogram model, integrating these values, supports tailored treatment plans and monitoring strategies in clinical settings. Patients with lower AR and LAAR have poorer survival outcomes, and the model's robust predictive capacity has been validated, enhancing precision in patient management and supporting individualized therapeutic decisions.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"5949-5961"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minimally invasive total hip arthroplasty versus minimally invasive hemiarthroplasty for geriatric femoral neck fractures: efficacy and impact on inflammatory and stress markers.","authors":"Linwu Li, Chaoxu Li, Dongxu Chen, Wei Peng, Mingzhou Chen, Shibin Su, Bin Huang","doi":"10.62347/TANY5623","DOIUrl":"10.62347/TANY5623","url":null,"abstract":"<p><strong>Objective: </strong>To compare the clinical efficacy, inflammatory responses, and surgical stress between minimally invasive total hip arthroplasty (MIS-THA) and minimally invasive hemiarthroplasty (MIS-HA) in the treatment of femoral neck fractures (FNFs) in elderly patients.</p><p><strong>Methods: </strong>From December 2018 to December 2021, 98 elderly patients with FNFs were enrolled, with 50 undergoing MIS-THA (observation group) and 48 undergoing MIS-HA (reference group). The clinical efficacy, surgery-related parameters (including intraoperative blood loss, postoperative drainage volume, and operation time), postoperative recovery (hip joint recovery time and hospitalization), and complication rates were compared between the two groups. Interleukin-6 (IL-6), C-reactive protein (CRP), Harris Hip Score, and Visual Analog Scale (VAS) scores were assessed preoperatively and postoperatively. Cortisol (Cor) and malondialdehyde (MDA) levels were measured at four time points: preoperatively (T0), immediately postoperatively (T1), and at 24 hours (T2) and 72 hours (T3) post-surgery. Additionally, efficacy comparisons were conducted across different age groups and fracture types.</p><p><strong>Results: </strong>The observation group demonstrated significantly higher treatment efficacy, shorter hip recovery time, and better functional outcomes (as evidenced by higher Harris and lower VAS scores) compared to the reference group. MIS-THA was associated with longer operative time, greater blood loss, and increased drainage volume (P<0.05), while hospitalization time and complication rates were comparable between groups (P>0.05). Postoperative IL-6 and CRP levels were elevated in both groups, but remained lower in the observation group (P<0.05). Stress markers (Cor and MDA) peaked at T2 and declined thereafter, with milder fluctuations observed in the observation group (P<0.05). The total effectiveness rate didn't differ significantly between the two groups across different age groups and fracture types.</p><p><strong>Conclusion: </strong>MIS-THA provides superior efficacy, faster functional recovery, and more pronounced pain relief, along with a milder inflammatory and stress response, compared to MIS-HA in elderly patients with FNFs. Despite longer operation time, greater intraoperative blood loss, and higher postoperative drainage, MIS-THA and MIS-HA exhibit similar safety profiles, as evidenced by comparable hospitalization durations and complication rates.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6113-6121"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral carbohydrate intake before selective laparoscopic liver resection reduces insulin resistance and enhances recovery.","authors":"Hongqiong Li, Yang Zhao, Yizheng Wang, Changlin Chen","doi":"10.62347/DMXL9003","DOIUrl":"10.62347/DMXL9003","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of preoperative oral carbohydrate administration on insulin resistance and recovery quality in patients undergoing selective laparoscopic liver resection.</p><p><strong>Methods: </strong>Data from 110 patients were retrospectively analyzed, and divided into a control group (n=55) and a research group (n=55). The control group received 800 mL of distilled water at 20:00 the evening before surgery, and 400 mL 2.5 hours before surgery. The research group received the same volume of carbohydrates at the same time points. Differences in blood glucose, insulin levels, insulin resistance (HOMA-IR), recovery quality (QoR-15), inflammatory and immune cell markers, and liver function were compared. Spearman correlation and logistic regression analyses were performed to explore the relationship between carbohydrate intake and recovery.</p><p><strong>Results: </strong>The research group had significantly lower incidence and severity of nausea and vomiting compared to the control group (χ²=0.037, χ²=0.030, both P<0.05). Postoperative recovery times, including post-anesthesia care, first rectal exhaust, and first feeding, were shorter in the research group (t=0.034, 0.021, 2.832, all P<0.05). Insulin levels and HOMA-IR were lower on postoperative days 1 and 3 in the research group (both P<0.05). QoR-15 scores were higher in the research group (F=100.100, P<0.001), showing an increasing trend over time (F=22.130, P<0.001). On day 3, inflammatory and liver function markers were lower in the research group, while immune cell markers were elevated (all P<0.05). Preoperative carbohydrate intake correlated with improved insulin sensitivity and recovery quality (P<0.05).</p><p><strong>Conclusion: </strong>Preoperative oral carbohydrate administration reduces insulin resistance, enhances recovery, and improves postoperative rehabilitation quality in patients undergoing elective laparoscopic liver resection.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6080-6091"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors and predictive nomogram for multi-organ failure in patients with acute kidney failure combined with severe sepsis.","authors":"Dongmei Yan, Jing Zhou, Hongying Zhang, Chaohua Peng","doi":"10.62347/JOZT7082","DOIUrl":"10.62347/JOZT7082","url":null,"abstract":"<p><strong>Objectives: </strong>To identify independent risk factors for multiple organ failure (MOF) and construct a clinically applicable predictive nomogram.</p><p><strong>Methods: </strong>We retrospectively analyzed 418 patients with acute kidney failure (AKF) and severe sepsis treated between January 2020 and September 2024. Demographic data, clinical features, and laboratory parameters were collected. Patients were randomly assigned to a training cohort (n=293) and a validation cohort (n=125). Independent risk factors for MOF were identified using logistic regression analysis, and a nomogram was subsequently developed. Model performance was evaluated using the area under the receiver operating characteristic curve (AUC), calibration curves, and decision curve analysis (DCA).</p><p><strong>Results: </strong>Five independent predictors of MOF were identified: abdominal infection, Acute Physiology and Chronic Health Evaluation II (APACHE II) score, neutrophil count (NEU), lactate (Lac), and heparin-binding protein (HBP). The nomogram showed good discrimination, with an AUC of 0.756 (95% CI: 0.701-0.811) in the training cohort and 0.816 (95% CI: 0.743-0.889) in the validation cohort. Calibration curves demonstrated good agreement between predicted and observed outcomes, and DCA indicated a favorable net clinical benefit.</p><p><strong>Conclusions: </strong>A nomogram incorporating abdominal infection, APACHE II score, NEU, Lac, and HBP effectively predicts the risk of MOF in AKF patients with severe sepsis. This model may aid in early risk stratification and clinical decision-making.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6141-6149"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"YiQiWenShen formula ameliorates myasthenia gravis through T follicular helper cells/T follicular regulatory cells immune rebalancing.","authors":"Kaihua Rao, Zhengfeng Li, Chunhua Huang","doi":"10.62347/CYDE1809","DOIUrl":"10.62347/CYDE1809","url":null,"abstract":"<p><strong>Background: </strong>Myasthenia gravis (MG), a chronic autoimmune neuromuscular disorder characterized by muscle weakness, remains a therapeutic challenge. This study aimed to evaluate the efficacy of the traditional Chinese herbal formulation YiQiWenShen (YQWS) in comparison with prednisone in a rat model of experimental autoimmune myasthenia gravis (EAMG), with the goal of exploring new therapeutic strategies.</p><p><strong>Methods: </strong>EAMG was induced in rats, who were subsequently treated with different doses of YQWS and prednisone. Clinical severity was assessed using the Baggi scoring system, alongside measurements of body weight, serum acetylcholine receptor antibody (AChR-Ab) levels, and splenic lymphocyte proliferation in response to the rat AChRα97-116 peptide and concanavalin A. Cytokine profiles, proportions of T follicular helper (Tfh) cells, T follicular regulatory (Tfr) cells, and germinal center B (GC)-B cells in lymph nodes were analyzed using enzyme-linked immunosorbent assay and flow cytometry. The expression levels of B-cell lymphoma 6 (Bcl-6) mRNA and protein were analyzed using quantitative real-time polymerase chain reaction and western blot, respectively.</p><p><strong>Results: </strong>After EAMG induction, the rats exhibited significant weight loss, elevated clinical scores, and increased AChR-Ab levels. Both YQWS and prednisone treatments significantly improved body weight and clinical scores, and reduced serum AChR-Ab levels. YQWS also attenuated the increased splenic lymphocyte proliferation. Cytokine dysregulation observed in EAMG rats was partially corrected following YQWS treatment. Furthermore, both YQWS and prednisone effectively normalized the Tfh/Tfr cell ratio, decreased GC-B cell populations, and downregulated Bcl-6 expression.</p><p><strong>Conclusion: </strong>YQWS exerts therapeutic effects in EAMG comparable to those of prednisone, demonstrating its potential as an alternative treatment for MG. Its efficacy is associated with the normalization of body weight, modulation of immune responses, correction of cytokine imbalances and reestablishment of Tfh/Tfr and GC-B cell homeostasis.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6333-6346"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}