American journal of translational research最新文献

{"title":"Clinical value of shear wave elastography (SWE) combined with superb microvascular imaging (SMI) for the evaluation of semen abnormalities.","authors":"Hailan Chen, Jianxin Huang, Wei Li, Hongsheng Chen, Jinqiang Peng, Chen Zeng","doi":"10.62347/KFDL8989","DOIUrl":"10.62347/KFDL8989","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical value of shear wave elastography (SWE) and superb microvascular imaging (SMI) in patients with semen abnormalities.</p><p><strong>Methods: </strong>A total of 150 patients with abnormal semen parameters who were treated at the Reproductive Center of the Affiliated Hospital of Putian University between January 2024 and December 2025 were retrospectively enrolled as the case group. In addition, 100 healthy individuals aged 18-35 were selected as the control group. SWE and SMI examinations were performed in both groups. Testicular volume and semen quality parameters were also measured. The diagnostic performance of SWE and SMI for semen abnormalities was evaluated using receiver operating characteristic (ROC) curve analysis. Spearman correlation analysis and one-way analysis of variance (ANOVA) were used to assess the correlation between SWE/SMI parameters and semen quality.</p><p><strong>Results: </strong>Bilateral testicular shear wave elastography values in the control group were significantly lower than thsoe in the case group (both <i>P</i><0.05). Bilateral microvascular grading based on SMI was significantly lower in the case group than the control group (χ²=153.344, 160.346, <i>P</i><0.05). LTSWE was significantly negatively correlated with left testicular volume, sperm concentration, total sperm count, semen volume, and total sperm motility (<i>P</i><0.05). Right testicular shear wave elastography (RTSWE) was significantly negatively correlated with total sperm motility. The area under the ROC curve (AUC) for LTSWE and RTSWE in diagnosing semen abnormalities was 0.805 and 0.626, respectively, while the combined AUC was 0.814. Left testicular SMI microvascular grading was related to LTSWE, total sperm count, and total sperm motility (<i>P</i><0.05). Right SMI microvascular grading was associated with RTSWE, sperm concentration, total sperm count, and total sperm motility (P<0.05). The sensitivity and specificity of SMI in diagnosing semen abnormalities were 83.33% and 96.0%, respectively. When combined with TSWE, the sensitivity and specificity increased to 87.33% and 99.0%, respectively.</p><p><strong>Conclusion: </strong>SWE and SMI are associated with semen quality parameters and demonstrate diagnostic value for semen abnormalities.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"7438-7448"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of flexible ureteroscopy assisted by a flexible-tip negative pressure suction sheath for 2-4 cm renal calculi.","authors":"Wei Tang, Guiqian Liu, Jiaxing Zhang, Bowen Chen, Zhisheng Pei, Weiqi Li, Yunbo Yang","doi":"10.62347/VLEP3960","DOIUrl":"10.62347/VLEP3960","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the efficacy and safety of flexible ureteroscopy assisted by a flexible-tip negative-pressure suction sheath for the treatment of 2-4 cm renal calculi.</p><p><strong>Methods: </strong>We retrospectively analyzed clinical data from 103 patients with 2-4 cm renal calculi who underwent flexible ureteroscopic holmium laser lithotripsy. Patients were divided into a flexible-tip suction sheath group (n=52) and a conventional sheath group (n=51) according to the type of ureteral access sheath used during surgery. Perioperative findings and surgical complications were compared between the two groups.</p><p><strong>Results: </strong>The stone-free rate was significantly higher in the flexible-tip suction sheath group (92.31%) than in the conventional sheath group (76.47%), and hospital stay was significantly shorter (both P < 0.05). The incidences of postoperative fever and steinstrasse in the flexible-tip suction sheath group were 3.85% and 1.92%, respectively, which were significantly lower than the 17.65% and 15.67% observed in the conventional sheath group (both P < 0.05). Postoperatively, serum levels of TNF-α, IL-1β, IL-6, CRP, cortisol, norepinephrine, and adrenocorticotropic hormone increased in both groups; however, the magnitude of elevation was significantly smaller in the flexible-tip suction sheath group (all P < 0.05). In addition, postoperative levels of serum creatinine, cystatin C, blood urea nitrogen, and neutrophil gelatinase-associated lipocalin, as well as 6-month postoperative quality-of-life scores, were significantly better in the flexible-tip suction sheath group than in the conventional sheath group (all P < 0.05).</p><p><strong>Conclusion: </strong>The application of flexible-tip negative-pressure suction sheath-assisted flexible ureteroscopy in patients with 2-4 cm renal calculi was associated with a higher stone-free rate, reduced incidences of specific postoperative complications, and more favorable short-term recovery outcome. This suggests its clinical value. Nevertheless, prospective randomized controlled trials are warranted for further validation.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6712-6722"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of analgesic and sedative effects of dexmedetomidine and propofol in patients with traumatic brain injury.","authors":"Xiaoping Shen, Jie Wang, Chao Zhang, Hui Cao, Baoqin Xu","doi":"10.62347/DRCZ2185","DOIUrl":"10.62347/DRCZ2185","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To assess the analgesic and sedative efficacy of dexmedetomidine (DEX) compared with propofol in individuals suffering from traumatic brain injury (TBI).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 126 participants with TBI and agitation, who were admitted to Suzhou Ninth Hospital Affiliated to Soochow University from January 2023 to January 2025, were enrolled in this study. According to the sedative agent assigned, these participants were categorized either into Group A (DEX) or Group B (propofol). Vital signs and blood gas measurements of the two cohorts were documented and contrasted before and after treatment. The Behavioral Pain Scale (BPS) and the Richmond Agitation-Sedation Scale (RASS) were employed for evaluation and comparison purposes. The frequencies of adverse reactions, including delirium, elevated blood pressure (BP), reduced BP, and bradycardia (slowed heart rate, HR), were monitored and contrasted between the two cohorts. The analgesic effectiveness of DEX was appraised in traumatic brain injury patients, alongside an evaluation of its consequences on vital signs, blood gas parameters, and safety profile. Records were kept regarding sedative and analgesic maintenance outcomes, sedation-linked adverse occurrences, and cerebral oxygen metabolism indices. Spearman correlation and logistic regression analyses were applied to determine factors influencing adverse events.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;At three hours following drug delivery, measurements of heart rate, mean arterial pressure, respiratory rate, and blood gas parameters in Group A were more favorable than those in Group B (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The proportion achieving target sedation and analgesic maintenance between 12 and 24 hours was elevated in Group A, whereas the numbers of analgesic rescue events and sedation modifications, together with the risks of patient-ventilator asynchrony, unplanned extubation, and excessive sedation, were diminished in Group A relative to Group B (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Cerebral oxygen metabolism remained more stable in Group A, accompanied by reduced concentrations of inflammatory cytokines and brain injury indicators, but elevated amounts of brain-derived neurotrophic factor (BDNF) (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Correlation analyses indicated that pain scores were linked to indicators of inflammation and neural injury. Regression analysis identified propofol, inadequate pain management, and sedation variability as independent risk determinants for adverse events. The overall incidence of adverse events in Group A was 9.30%, which was markedly lower than the 65.00% observed in Group B (&lt;i&gt;P&lt;/i&gt;&lt;0.001).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;In agitated patients with TBI, dexmedetomidine was associated with improved sedative and analgesic effects, more stable sedation maintenance, reduced need for additional clinical interventions, and better preservation of respiratory and hemodynamic stability, as well as cerebral oxygen metabolic bal","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6901-6915"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of systemic vascular resistance index-guided versus conventional fluid management on airway pressure, oxygenation and postoperative recovery in patients undergoing one-lung ventilation for thoracic surgery.","authors":"Rui Qiu, Na He, Wei He","doi":"10.62347/DAUL3237","DOIUrl":"10.62347/DAUL3237","url":null,"abstract":"<p><strong>Objective: </strong>To compare the effects of systemic vascular resistance index (SVRI)-guided goal-directed fluid therapy (GDFT) versus conventional fluid management on perioperative airway pressure, oxygenation, and postoperative recovery in thoracic surgery patients with one-lung ventilation (OLV), and to identify independent risk factors for postoperative pulmonary complications (PPCs).</p><p><strong>Methods: </strong>A total of 386 patients were retrospectively enrolled and randomly split into a training set (n=270) and a validation set (n=116) at a 7:3 ratio. The training set was divided into SVRI group (n=155) and control group (n=115). Baseline data, airway pressure, oxygenation, recovery indicators, and 7-day PPCs were collected. Independent risk factors were screened by logistic regression; a nomogram was constructed and evaluated by ROC and calibration curves.</p><p><strong>Results: </strong>Baseline characteristics were comparable between the groups (P>0.05). The SVRI group had lower airway pressure, fluid volume, infusion rate, recovery time, and PPCs incidence, as well as better oxygenation and urine output (all P<0.05). Ppeak ≥26.845 cmH₂O, Pplat ≥21 cmH₂O, ΔP ≥14.945 cmH₂O, and PaO₂/FiO₂ <303.815 mmHg were independent risk factors for PPCs. The combined four-index model showed better predictive efficiency.</p><p><strong>Conclusion: </strong>SVRI-guided GDFT improves perioperative outcomes and reduces PPCs in OLV patients. The four indicators effectively predict PPCs, and their combined detection enhances risk stratification accuracy.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"7285-7296"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Huaier Granules combined with transarterial chemoembolization on recurrence and survival in postoperative hepatocellular carcinoma patients with microvascular invasion.","authors":"Xiaochun Liu, Yanan Li, Fangrong Liang, Lei Gou, Yue Liang, Hong Li","doi":"10.62347/FUOU7602","DOIUrl":"10.62347/FUOU7602","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate whether Huaier Granules combined with transcatheter arterial chemoembolization (TACE) reduces recurrence and improves survival in patients with microvascular invasion (MVI)-positive primary hepatocellular carcinoma (HCC) after resection.</p><p><strong>Methods: </strong>A single-center retrospective study enrolled 266 patients (2019-2021), who were divided into the Huaier Granules + TACE group (Observation Group) (n=137) and the TACE alone group (Control Group) (n=129). Key outcomes included recurrence-free survival (RFS), overall survival (OS), objective response rate (ORR), liver function, adverse events (AEs), and health-related quality of life (HRQoL) assessed by the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire Hepatocellular Carcinoma 18-question module. Survival was analyzed using the Kaplan-Meier method; Cox regression and receiver operating characteristic (ROC) analysis were employed to explore prognostic factors and evaluate discrimination.</p><p><strong>Results: </strong>Compared with TACE alone, the combination group had a higher ORR (55.47% vs 38.76%, P<0.005), lower 1-/2-/3-year recurrence rates (16.79%/44.53%/78.10% vs 26.36%/53.48%/89.15%, P<0.05), and longer RFS and OS (both P<0.05). The combination group exhibited more significant improvements in alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil), and albumin (ALB) (all P<0.05), with a comparable AE rate (P>0.05) and better 3-month HRQoL (all P<0.05). Multivariable analysis identified Tumor-Node-Metastasis (TNM) stage, post-treatment AST, and treatment modality as independent predictors of 3-year mortality, with modest area under the curve values (0.572-0.699).</p><p><strong>Conclusion: </strong>Huaier Granules combined with TACE was associated with reduced recurrence, prolonged survival, favorable safety, and better short-term HRQoL in this cohort. These findings require cautious interpretation and prospective validation due to the study's limitations.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6875-6888"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated P-RBC complexes are associated with thrombotic risk and diagnostic potential in myeloproliferative neoplasms: a retrospective cohort study.","authors":"Xiaonan Niu, Caihong Jia, Yuefan Ma, Xuezhi Huang, Chao Li","doi":"10.62347/LVNO6845","DOIUrl":"10.62347/LVNO6845","url":null,"abstract":"<p><strong>Background: </strong>Myeloproliferative neoplasms (MPNs) are clonal hematopoietic stem cell disorders characterized by excessive myeloid proliferation. The impaired clearance of senescent erythrocytes is a key pathological feature, yet its mechanisms remain unclear. Pro-phagocytic platelet-erythrocyte (P-RBC) complexes may represent a novel intercellular interaction involved in this process.</p><p><strong>Objective: </strong>To investigate the levels of P-RBC complexes in MPN patients, their association with clinical characteristics and thrombotic risk, and their potential diagnostic value.</p><p><strong>Methods: </strong>In this retrospective cohort study, 152 MPN patients (including polycythemia vera [PV], essential thrombocythemia [ET], and primary myelofibrosis [PMF]) and 152 healthy controls were enrolled. P-RBC complex levels in peripheral blood were measured using flow cytometry. Correlations with laboratory parameters, thrombotic events, and survival were analyzed.</p><p><strong>Results: </strong>P-RBC complex levels were significantly higher in MPN patients than in controls (2.76 ± 0.53% vs. 1.28 ± 0.37%, P < 0.001). Levels varied by subtype, with PMF patients showing the highest levels (3.28 ± 0.52%), followed by PV (2.76 ± 0.44%) and ET (2.49 ± 0.39%) (P < 0.001). P-RBC levels positively correlated with hemoglobin (r = 0.456), C-reactive protein (r = 0.291), and lactate dehydrogenase (r = 0.736) (all P < 0.001). Patients with thrombotic events had higher P-RBC levels than those without (3.04 ± 0.70% vs. 2.72 ± 0.48%, P = 0.009). Multivariate analysis identified P-RBC level as an independent risk factor for thrombosis (OR = 3.07, P = 0.012). Although survival was worse in the high-P-RBC group, the difference was not statistically significant (P = 0.194). ROC analysis showed excellent diagnostic performance for MPNs (AUC = 0.990), with 100.0% sensitivity and 91.4% specificity at a 1.80% cutoff.</p><p><strong>Conclusion: </strong>P-RBC complexes are significantly elevated in MPN patients and correlate with disease subtype, inflammation, and thrombotic risk. They represent a promising novel biomarker for MPN diagnosis and risk stratification.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6535-6549"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13589834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental pathologic score 1-2 subclinical abnormalities and early neonatal clinical indicators in term infants: a retrospective study.","authors":"Xinwu Mao, Fanbin Ye, Haidi Chang, Xiaowen Zhang, Tingting Cheng","doi":"10.62347/VVZM8791","DOIUrl":"10.62347/VVZM8791","url":null,"abstract":"<p><strong>Objective: </strong>To explore the association between placental pathology scores of 1-2 and early neonatal clinical indicators, and evaluate the independent predictive effect of such abnormalities on composite adverse outcomes.</p><p><strong>Methods: </strong>This single-center retrospective case-control study enrolled 125 term singleton live births that underwent placental pathologic examination. Based on Amsterdam Consensus scoring criteria, neonates were assigned to abnormal (score 1-2, n = 63) or control (score 0, n = 62) groups. Neonatal clinical indicators within 7 days postpartum were compared between groups, followed by multivariate logistic regression and receiver operating characteristic (ROC) curve analyses.</p><p><strong>Results: </strong>Baseline maternal and neonatal characteristics were balanced across the two groups (all P>0.05). The abnormal group exhibited a markedly higher composite adverse outcome rate (50.8% vs. 19.4%, P<0.001), lower umbilical artery pH (7.25±0.07 vs. 7.31±0.05, P<0.001), a higher acidosis rate (19.0% vs. 0.0%, P<0.001), lower 1-h blood glucose (3.18±0.65 vs. 3.48±0.67 mmol/L, P = 0.012), and elevated C-reactive protein (CRP) levels (8.94±7.42 vs. 3.61±2.78 mg/L, P<0.001) alongside a higher proportion of CRP >10 mg/L (34.9% vs. 4.8%, P<0.001). No significant intergroup differences were observed in the incidence of clinical hypoglycemia (19.0% vs. 11.3%, P = 0.338) or intravenous antibiotic administration (3.2% vs. 4.8%, P = 0.680). Multivariate logistic regression confirmed that placental subclinical abnormalities constituted an independent risk factor for composite adverse outcome (adjusted OR = 3.779, 95% CI: 1.499-9.529, P = 0.005). The area under the ROC curve was 0.672.</p><p><strong>Conclusions: </strong>Placental pathology scores of 1-2 are independently associated with neonatal acidosis and elevated inflammatory markers in term neonates, and correlate with a nearly fourfold increased risk of composite adverse outcome. Mild placental subclinical abnormalities should be integrated into routine neonatal risk assessment to optimize perinatal transitional care.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6505-6516"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13589835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A <i>CHD7</i> intronic variant induces aberrant splicing and structural alterations in the CHD7 DNA-binding domain to cause CHARGE syndrome.","authors":"Yangfan Fei, Dan Hu, Lyu Liu, Xianglian Tian, Jinbao Chai, Lixia Wu, Aiping Li, Qinglian Zhang, Daibo Li","doi":"10.62347/UFCX1012","DOIUrl":"10.62347/UFCX1012","url":null,"abstract":"<p><strong>Objectives: </strong>CHARGE syndrome (CS), a rare inherited condition, is mainly caused by pathogenic variants in the gene encoding chromodomain helicase DNA-binding protein 7 (<i>CHD7</i>). In the present investigation, the genetic etiology of CS was elucidated in a Chinese patient presenting with characteristic clinical features.</p><p><strong>Methods: </strong>A 28-year-old female with amenorrhea, facial asymmetry, right ear malformation, hearing impairment, and congenital cardiac anomalies was evaluated using whole-exome sequencing (WES) for mutation screening. Familial Sanger sequencing subsequently confirmed the identified variant as <i>de novo</i>. Minigene assays were used to assess the pathogenicity of the splicing variant, while the structural alterations in CHD7 were characterized using computational modeling and electrostatic potential analyses.</p><p><strong>Results: </strong>WES detected a heterozygous <i>CHD7</i> splice-site variant, c.5405-17 G>A, which was not detected in either parent. Minigene-based functional assessments revealed this <i>de novo CHD7</i> variant to cause a 15-bp insertion, resulting in a five-amino-acid insertion between the helicase and the SANT domains of CHD7, thereby potentially altering DNA binding. Hormone replacement therapy successfully induced cyclic menstruation, and surgical intervention corrected the auricular deformity.</p><p><strong>Conclusions: </strong>A <i>de novo</i> pathogenic splice-site mutation (c.5405-17 G>A) was identified in this study, which leads to the pathogenesis of CS. Subsequent functional evaluation confirmed that this mutation disrupts normal mRNA processing, thereby inducing structural alterations in the CHD7 protein. The results obtained herein advance our understanding of the mutational spectrum of <i>CHD7</i>, while emphasizing the importance of combining clinical, genetic, and functional analyses for the definitive diagnosis of CS.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6662-6671"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydatin ameliorates alcohol-induced gastric ulcer by inhibiting CASP3-mediated apoptosis.","authors":"Lei Zhu, Yexin Tang, Qinglan Song, Yongbo Wang","doi":"10.62347/ECGE1624","DOIUrl":"10.62347/ECGE1624","url":null,"abstract":"<p><strong>Objective: </strong>To explore the protection of polydatin (PD) against alcoholic gastric ulcer (AIGU) in mice and the related molecular pathways.</p><p><strong>Methods: </strong>PD targets related to AIGU were predicted using network pharmacology, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed. A mouse gastric ulcer model was established via intragastric administration of ethanol, and the effects of PD on gastric mucosal morphology, oxidative damage, inflammation, and apoptosis-related proteins were evaluated; In vitro, the effects of PD on ethanol-injured GES-1 cells were further assessed in the presence or absence of the caspase-3 inhibitor Z-DEVD-FMK.</p><p><strong>Results: </strong>Network pharmacology showed that PD exerts its effects through apoptosis-related pathways. In vivo, PD significantly alleviated gastric mucosal injury, reduced the levels of malondialdehyde and pro-inflammatory cytokines, and increased the activities of antioxidant enzymes. It up-regulated mucosal protection factors (epidermal growth factor, prostaglandin E₂) and regulates apoptosis-related proteins (B-cell lymphoma-2, B-cell lymphoma-2-associated X protein, cysteinyl aspartate-specific proteinase-3). In vitro, PD protects gastric mucosal epithelial cells from apoptosis; the caspase-3 inhibitor Z-DEVD-FMK alone mimicked the protective effects of PD, and combined treatment with PD and Z-DEVD-FMK did not produce a markedly additive effect, suggesting that PD exerts its anti-apoptotic effect primarily through inhibition of caspase-3 (CASP3)-mediated apoptosis.</p><p><strong>Conclusion: </strong>PD exerts antioxidant, anti-inflammatory, and anti-apoptotic effects against AIGU, especially inhibiting CASP3-mediated apoptosis, which shows its potential as a natural gastric protection therapy.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6850-6865"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of endoscopic transnasal orbital decompression on anterior segment ocular blood flow and clinical outcome in thyroid-associated ophthalmopathy: a retrospective study.","authors":"Qian Xiao, Suhui Zhu, Baozhu Dai, Yusheng Zhao, Xiaohu Chen, Yan Dai","doi":"10.62347/WWVM4417","DOIUrl":"10.62347/WWVM4417","url":null,"abstract":"<p><strong>Background: </strong>Thyroid-associated ophthalmopathy (TAO) is characterized by orbital inflammation and elevated intraorbital pressure, which may compromise anterior segment microcirculation. While endoscopic transnasal orbital decompression (ETOD) effectively alleviates proptosis and improves vision, its effect on microcirculation remains underexplored.</p><p><strong>Objective: </strong>This study aimed to evaluate the effect of ETOD on anterior segment microcirculation in TAO using SS-OCTA and to correlate these changes with clinical outcome.</p><p><strong>Methods: </strong>This retrospective study included 45 TAO patients undergoing ETOD and 45 matched healthy controls. SS-OCTA measures (superficial, deep, and small vessel density; blood flow area) and clinical measures (exophthalmos, intraocular pressure, best-corrected visual acuity) were assessed preoperatively and 3 months postoperatively.</p><p><strong>Results: </strong>Preoperatively, SVD was significantly higher in TAO patients than in healthy controls (50.63 ± 1.98% vs. 48.53 ± 2.78%, P < 0.001), whereas DVD showed no significant difference (47.93 ± 1.59% vs. 47.30 ± 2.57%, P = 0.169). In contrast, BFA was significantly lower (2.07 ± 0.31 vs. 2.49 ± 0.27 mm², P < 0.001), whereas SmiVD showed no significant difference (P = 0.939). Three months after ETOD, SVD and DVD decreased significantly (temporal quadrant SVD: from 51.53 ± 2.26% to 43.37 ± 1.78%, -15.8%, P < 0.001; temporal DVD: from 47.81 ± 2.49% to 38.66 ± 2.70%, -19.1%, P < 0.001), whereas BFA increased significantly (temporal BFA: from 2.08 ± 0.49 mm² to 2.34 ± 0.45 mm², +12.5%, P < 0.001). SmiVD remained unchanged (P > 0.05). BCVA showed no significant change (LogMAR 0.05 ± 0.06 to 0.04 ± 0.07, P = 0.290). Exophthalmos reduction showed a significant positive correlation with SVD reduction (r = 0.346, P = 0.020), and postoperative IOP was not significantly correlated with postoperative SmiVD (r = 0.024, P = 0.875).</p><p><strong>Conclusions: </strong>ETOD effectively reduced exophthalmos and IOP and restored anterior segment hemodynamics by decreasing pathologically elevated vessel densities toward normal levels. SS-OCTA provides a valuable noninvasive tool for monitoring microcirculatory changes after orbital decompression in TAO.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"18 8","pages":"6988-6998"},"PeriodicalIF":1.8,"publicationDate":"2026-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13590796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}