American journal of translational research最新文献

{"title":"Minimally invasive triumph: the role of thoracic duct embolization in managing severe post-esophagectomy chylothorax.","authors":"Rong Yan, Shan Gao, Peng Xia","doi":"10.62347/XINP3272","DOIUrl":"10.62347/XINP3272","url":null,"abstract":"<p><p>Chylothorax, a rare and severe complication of esophageal cancer surgery, results from damage to the lymphatic system. We report on a 70-year-old female with esophageal cancer and a history of poorly managed diabetes who developed high-flow chylothorax following esophagectomy. Initial conservative measures, including pleural drainage, lymph production reduction medication, and nutritional support, failed to improve her condition. Subsequent surgical attempts to ligate the thoracic duct were also unsuccessful due to extensive pleural adhesions. This led to a referral for percutaneous thoracic duct embolization (TDE). At another medical facility, the patient underwent lymphangiography, cisterna chyli puncture embolization, and cavity puncture drainage. The TDE successfully embolized the thoracic duct, markedly reducing the leakage of chylous fluid. Subsequent CT scans and follow-up assessments confirmed the patient's recovery with no recurrence of chylothorax. This case illustrates the complexities of managing post-esophagectomy chylothorax and highlights the importance of individualized treatment strategies. It also emphasizes the potential of minimally invasive TDE as an effective alternative for treatment-resistant chylothorax cases.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6610-6618"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alcohol promoted prostate microbial imbalance in the rat model of prostatitis.","authors":"Xin Zhu, Ping Xu, Yandong He, Wenlong Lu, Zhong Wang, Feng Liu","doi":"10.62347/CRGN1110","DOIUrl":"10.62347/CRGN1110","url":null,"abstract":"<p><strong>Objective: </strong>Alcohol may aggravate the clinical symptoms of chronic prostatitis (CP)/chronic pelvic pain syndrome (CPPS), but the molecular mechanism behind this connection have not been fully understood. In our study, we established a rat model of experimental autoimmune prostatitis (EAP) to investigate the impact of alcohol exposure on the changes in prostatic microbiota.</p><p><strong>Methods: </strong>The EAP rat model was established using prostate steroid-binding protein with subsequently administered alcohol exposure. The concentration of alcohol was quantified by a standard alcohol concentration assay. The inflammatory factors were measured through enzyme-linked immunosorbent assay (ELISA). Subsequently, the composition and diversity of the prostate microbiota were analyzed using 16S rRNA gene sequencing data.</p><p><strong>Results: </strong>Elevated levels of inflammatory factors and morphological characteristics of prostate tissue confirmed that the EAP rat model was successfully established. Following alcohol exposure, a significant increase in blood alcohol concentration was observed. Alcohol exposure further exacerbated dysbiosis in prostate microbiota, altering microbial abundance, evenness, and composition in EAP rats. More than 50 metabolic pathways related to biosynthesis, degradation/utilization/assimilation, detoxification, generation of prostate metabolite and energy, macromolecule modification, glycan pathways and metabolic clusters were predicted to be disrupted. Additionally, metabolomics profiling revealed that alcohol impaired pathways such as PWY-6876, PWY-6339, PWY-722, and PWY-5177, which were strongly associated with microbial changes, including <i>Streptomyces, Oscillospira, Pseudomonas, Lactobacillus</i>, unidentified-<i>Clostridiales</i>, and unclassified-Bacteria.</p><p><strong>Conclusion: </strong>Our findings suggested that alcohol exacerbates prostatitis by disrupting the balance of prostate microbiota. This finding could provide valuable insights for improving the diagnosis and treatment for patients with alcoholic prostatitis.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"5914-5927"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432687/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of platelet-rich fibrin combined with low-level erbium laser on soft and hard tissues and bone regeneration around implants.","authors":"Xiaoling Zhang, Liangzhi Du, Ningbo Zhao, Lizhe Zhu, Lei Wang, Xiaofeng Chang","doi":"10.62347/QJPT4439","DOIUrl":"10.62347/QJPT4439","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effects of adjunctive low-energy erbium-doped yttrium aluminum garnet (Er:YAG) laser therapy combined with platelet-rich fibrin (PRF) on peri-implant tissue healing, implant stability, bone regeneration, and postoperative inflammation in dental implant patients.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted with 171 patients who underwent dental implant placement from November 2020 to October 2024. Patients were divided into PRF (PRF alone, n=92) and PRF-ER (PRF combined with low-energy Er:YAG laser therapy, n=79). Clinical parameters, including modified plaque index (mPI), modified sulcus bleeding index (mSBI), probing depth (PD), and implant stability quotient (ISQ) by resonance frequency analysis, were assessed at 2 weeks, 6 weeks, and 3 months post-implantation. Peri-implant crevicular fluid was collected for osteoprotegerin (OPG) quantification. Radiographic assessments of bone density (BD) and ridge measurements were performed using cone-beam computed tomography at baseline and 3 months. Postoperative inflammation and healing were evaluated by visual inspection and the Landry Index.</p><p><strong>Results: </strong>At 3 months postoperatively, the PRF-ER group showed significantly lower mPI, mSBI, and PD, and higher ISQ compared to the PRF group (all P<0.05). OPG levels were significantly higher in the PRF-ER group at 3 months, as were BD, horizontal ridge, and vertical ridge measurements (all P<0.05). Soft tissue thickness remained similar. A greater proportion of PRF-ER patients showed no inflammation and optimal healing.</p><p><strong>Conclusion: </strong>Adjunctive low-energy Er:YAG laser therapy with PRF significantly improves peri-implant tissue healing, implant stability, bone regeneration, and reduces postoperative inflammation compared to PRF alone in dental implant patients. These findings support the clinical utility of combined modality therapy for enhancing peri-implant outcomes.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6042-6055"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of ACTH therapy in treating infantile epileptic spasm syndrome and its effect on Kramer score.","authors":"Fengtong Li, Dongqin Bai, Juanli Li, Qiang Yuan, Xuehong Wang, Jumei Qu","doi":"10.62347/EUPP5177","DOIUrl":"10.62347/EUPP5177","url":null,"abstract":"<p><strong>Objective: </strong>To compare the clinical efficacy and safety of adrenocorticotropic hormone (ACTH) combined with topiramate versus topiramate monotherapy in treating Infantile Epileptic Spasm Syndrome (IESS), and to explore the risk factors for relapse.</p><p><strong>Methods: </strong>This retrospective cohort study included 185 IESS pediatric patients treated at Yan'an People's Hospital between April 2018 and January 2024. Patients were divided into a control group (n=95, topiramate monotherapy) and an observation group (n=90, ACTH combined with topiramate) based on their treatment regimen. Propensity score matching (PSM) was employed to balance baseline differences. Outcomes compared between the groups included spasm control rates, electroencephalogram (EEG) improvement, changes in functional scores, and incidence of adverse reaction. Multivariate logistic regression was used to identify independent risk factors for relapses.</p><p><strong>Results: </strong>The observation group demonstrated significantly higher rates of complete spasm control (75.56% vs. 50.53%) and complete EEG improvement (76.67% vs. 54.74%) compared to the control group (P<0.01). Post-treatment, the observation group showed more significant improvements in developmental quotient (DQ), motor index (MI), and Kramer scores (P<0.01). Multivariate analysis identified structural etiology (OR=3.12), monotherapy (OR=2.54), and higher Kramer scores (OR=1.45) as independent risk factors for relapse (all P<0.01). The incidence of adverse reactions did not differ significantly between groups (34.44% vs. 29.47%, P=0.468).</p><p><strong>Conclusion: </strong>ACTH combined with topiramate offers superior efficacy for spasm control, EEG improvement, and neurological function recovery compared to topiramate monotherapy, without increasing adverse reactions. Structural etiology, monotherapy, and Kramer scores are independent predictors for relapse.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6150-6165"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined trigger point acupuncture knife and traditional Chinese medicine split-tendon massage for shoulder periarthritis: improved function and quality of life.","authors":"Shijian Wang, Boxu Lang, Weiguang Mou, Yongjiu Wang, Xinxin Chen, Jiawang Lang","doi":"10.62347/DIDZ9909","DOIUrl":"10.62347/DIDZ9909","url":null,"abstract":"<p><strong>Objectives: </strong>To compare the effectiveness of combining trigger point acupuncture knife (TPAK) with Traditional Chinese Medicine (TCM) split-tendon massage therapy versus TPAK alone.</p><p><strong>Methods: </strong>A retrospective study included 237 patients diagnosed with shoulder periarthritis. Of these, 114 patients received only TPAK therapy (TPAK Group), while 123 patients underwent a combination of TPAK and TCM split-tendon massage therapy (TPAK + TCM Group). Shoulder function was assessed using the Constant-Murley Score (CMS) and shoulder range of motion (ROM). Pain levels were evaluated using the Short-Form McGill Pain Questionnaire (SF-MPQ). Psychological status, sleep quality, and overall quality of life were measured using the WHOQOL-BREF questionnaire, both before and 3 months after treatment.</p><p><strong>Results: </strong>Both groups showed improvements across all outcomes, with the TPAK + TCM group showing significantly better results than the TPAK group. CMS scores revealed greater improvement in shoulder function and pain relief in the TPAK + TCM group, particularly in affective pain dimensions (<i>P</i> < 0.001). Additionally, the TPAK + TCM group exhibited greater reductions in anxiety and depression, as well as improvements in sleep quality and overall physical and psychological health (all <i>P</i> < 0.05), compared to the TPAK group.</p><p><strong>Conclusion: </strong>Combining TPAK with TCM split-tendon massage proved more effective than TPAK alone in treating shoulder periarthritis, improving function, reducing pain, enhancing psychological well-being, and improving sleep quality.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6652-6662"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternating intravesical instillation of Bacillus Calmette-Guérin and <i>Pseudomonas aeruginosa</i> effectively prevents postoperative recurrence in high-risk non-muscle-invasive bladder cancer.","authors":"Yiqun Shao, Yongjun Guan, Jingying Zhao, Mierxiati Abudurexiti, Zhong Wang","doi":"10.62347/GSYN6858","DOIUrl":"10.62347/GSYN6858","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the role of alternating intravesical instillation of Bacillus Calmette-Guérin (BCG) and <i>Pseudomonas aeruginosa</i> (PA) in preventing postoperative recurrence in high-risk non-muscle-invasive bladder cancer (HR-NMIBC).</p><p><strong>Methods: </strong>We retrospectively reviewed the clinical data from 115 HR-NMIBC cases who underwent transurethral resection of bladder tumors (TURBT) at Gongli Hospital of Shanghai Pudong New Area between March 2021 and January 2023. Patients were grouped based on postoperative management: a control group (n=51) treated with standard gemcitabine instillations and an intervention group (n=64) given alternating BCG and PA instillations. This study assessed 1- and 2-year recurrence, recurrence-free survival, safety (gastrointestinal reactions, fever, bladder irritation symptoms, and hematuria), serum tumor markers, and life quality. Univariate and multivariate Cox proportional hazards analyses were applied to identify the recurrence predictors. A nomogram predictive model was further developed for postoperative recurrence risk estimation, and its performance was later validated.</p><p><strong>Results: </strong>Despite an equivalent 1-year recurrence rate, the intervention group showed a lower 2-year recurrence rate, prolonged recurrence-free survival, and superior safety (fewer adverse events) than controls. The intervention group also showed decreased post-treatment serum tumor marker concentrations and greater life quality enhancement relative to the control cohort. Univariate and multivariate analyses identified tumor number ≥3 (P=0.036), high-grade tumors (P=0.040), and gemcitabine monotherapy (P=0.035) as independent predictors for 2-year recurrence. The nomogram's scoring system reliably associated elevated risk points with heightened recurrence risk, demonstrating strong discrimination and reliable calibration in medium-to-high-risk ranges.</p><p><strong>Conclusions: </strong>Alternating intravesical BCG and PA instillations markedly decreases 2-year postoperative recurrence on the premise of favorable safety in HR-NMIBC patients.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6619-6629"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the development and evaluation of animal models for chronic fatigue syndrome.","authors":"Chuwen Feng, Yongfang Wu, Weibo Sun, Yuanyuan Qu, Miao Zhang, Tiansong Yang","doi":"10.62347/RYAU2739","DOIUrl":"10.62347/RYAU2739","url":null,"abstract":"<p><p>Chronic fatigue syndrome (CFS) is a debilitating multisystem disorder with an increasing prevalence, significantly impairing patients' quality of life. Several challenges remain, including unclear pathogenesis, the absence of specific diagnostic criteria, and limited therapeutic efficacy. Animal models play a crucial role in understanding the pathogenesis of CFS and testing potential therapies, necessitating the construction and evaluation of these models to be objective and scientifically rigorous. This study provides a comprehensive review of the methods for developing and assessing CFS models, analyzes their strengths and limitations, identifies key challenges in current research, and guides the development of clinically relevant CFS animal models.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"5848-5861"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic outcomes and inflammatory modulation of inhaled N-acetylcysteine bronchoalveolar lavage in severe pneumonia.","authors":"Shuzhen Long, Huazhao Qin, Jing Guo, Lihua Liu","doi":"10.62347/CDJX3226","DOIUrl":"10.62347/CDJX3226","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the therapeutic efficacy of inhaled N-acetylcysteine (NAC) bronchoalveolar lavage (iNAC-BAL) in severe pneumonia (SP) and explore its effects on inflammatory cytokines modulation.</p><p><strong>Methods: </strong>A total of 146 SP cases were assigned to two groups: the control group received bronchoalveolar lavage with isotonic saline (0.9% NaCl) alone, while the observation group received additional NAC aerosol inhalation. Clinical efficacy, Acute Physiology and Chronic Health Evaluation (APACHE) II scores, intensive care unit (ICU) stay, duration of ventilator dependence, adverse reactions, symptom resolution times, respiratory mechanics, pulmonary function, inflammatory cytokines, and humoral immunity were assessed and compared between the two groups.</p><p><strong>Results: </strong>The observation group achieved better therapeutic effects (P=0.007), with significantly lower APACHE II scores, shorter ICU stays, reduced ventilator dependence, faster symptom resolution, and fewer adverse events (all P<0.05). Additionally, respiratory dynamics, lung function, inflammatory cytokines, and humoral immunity improved markedly in the observation group compared with the control group (all P<0.05).</p><p><strong>Conclusion: </strong>iNAC-BAL demonstrates significant clinical efficacy and potent anti-inflammatory effects in the management of SP.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6630-6638"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalein inhibits DDX60 to suppress pancreatic cancer growth and regulate the tumor microenvironment.","authors":"Lanying Song, Renming Cai","doi":"10.62347/TTQJ2494","DOIUrl":"10.62347/TTQJ2494","url":null,"abstract":"<p><strong>Objective: </strong>To explore the effects of baicalein on immune cell infiltration and tumor progression in pancreatic cancer by modulating DDX60 expression.</p><p><strong>Methods: </strong>RNA-seq data of pancreatic cancer and normal tissues were obtained from the UCSC XENA database. DDX60 expression differences and their associations with patient prognosis and immune infiltration were analyzed. Panc02 pancreatic cancer cells were treated with baicalein (0, 20, 40, 60 μmol/L) for 24, 48, and 72 hours. Cell viability was assessed by MTT assay, while apoptosis and DDX60 expression were evaluated by flow cytometry and RT-qPCR, respectively. In vivo, tumor-bearing mice received baicalein, and tumor volume, immune cell infiltration, and DDX60 expression in tumor tissues were assessed.</p><p><strong>Results: </strong>DDX60 expression was significantly upregulated in pancreatic cancer tissues compared to normal tissues (P < 0.05). Patients with low DDX60 had better survival (P < 0.05). DDX60 levels correlated significantly with multiple immune cell types, including DCs, eosinophils, macrophages, neutrophils, T cell subsets, and NK cells (P < 0.05). Baicalein inhibited Panc02 cell proliferation and induced apoptosis in a dose- and time-dependent manner (P < 0.05), accompanied by downregulation of DDX60 (P < 0.05). In vivo, baicalein significantly suppressed tumor growth and increased CD8⁺ T cells and macrophages in tumor tissues (P < 0.05). DDX60 expression decreased with increasing baicalein dosage (P < 0.05).</p><p><strong>Conclusion: </strong>Baicalein suppresses pancreatic cancer growth and promotes apoptosis, apparently through downregulation of DDX60 and modulation of immune responses in the tumor microenvironment.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"5885-5895"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432716/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered expression of miR-132, miR-155, VEGF, and IGF-1 as key indicators in the pathogenesis of diabetic foot infection.","authors":"Pengbo Yang, Guanwen Sun, Huhe Bao, Wanyin Zhang, Lihang Wang, Fei Huang, Yaxing Zhang","doi":"10.62347/ILFJ9140","DOIUrl":"10.62347/ILFJ9140","url":null,"abstract":"<p><strong>Aims: </strong>This study aimed to investigate the alterations of miR-132, miR-155, vascular endothelial growth factor (VEGF), and insulin-like growth factor-1 (IGF-1) expression in patients with diabetic foot infection (DFI), and to analyze and the distribution of pathogenic microorganisms, with the goal of elucidating underlying molecular mechanisms and identifying potential diagnostic and therapeutic biomarkers.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 114 patients with diabetic foot, who were divided into two groups: an observation group (n = 56) comprising patients with DFI, and a control group (n = 58) without infection. The primary outcomes included the expression levels of miR-132, miR-155, VEGF, and IGF-1, as well as microbial profiles isolated from infected wound sites. Secondary outcomes encompassed inflammatory markers [C-reactive protein (CRP), white blood cell count (WBC), interleukin-6 (IL-6), procalcitonin (PCT)], biochemical markers [fasting glucose, glycated hemoglobin (HbA1c), lipid profile], and hormonal markers [cortisol, thyroid-stimulating hormone (TSH), growth hormone (GH), and leptin].</p><p><strong>Results: </strong>Microbiological cultures identified Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli as the predominant pathogens in DFI cases, with a mixed infection observed in a subset. Expression levels of miR-132 (5.6 ± 1.2 vs. 2.3 ± 0.5, P < 0.001), miR-155 (4.8 ± 1.1 vs. 1.9 ± 0.6, P < 0.001), VEGF (245.3 ± 32.5 pg/mL vs. 150.7 ± 25.3 pg/mL, P < 0.001), and IGF-1 (182.4 ± 30.6 pg/mL vs. 124.8 ± 21.7 pg/mL, P < 0.001) were significantly upregulated in the observation group compared to controls, suggesting their involvement in the pathogenesis of DFI. Inflammatory and biochemical markers were markedly elevated in the observation group (P < 0.001), reflecting systemic inflammation and metabolic dysregulation. Hormonal analysis revealed increased cortisol and insulin levels, along with decreased TSH, GH, and leptin levels (P < 0.001). Additionally, significant negative correlations were found between miR-132/miR-155 and VEGF/IGF-1, indicating potential regulatory interactions in the context of inflammation and vascular remodeling (P < 0.001).</p><p><strong>Conclusion: </strong>Elevated expression of miR-132, miR-155, VEGF, and IGF-1, together with characteristic microbial profiles, plays a critical role in the pathogenesis of DFI. These molecules may serve as promising biomarkers for disease diagnosis and therapeutic monitoring.</p>","PeriodicalId":7731,"journal":{"name":"American journal of translational research","volume":"17 8","pages":"6092-6102"},"PeriodicalIF":1.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}