Journal of experimental animal science最新文献_第2页

Characterization of a rat model of right-sided heart failure induced by pulmonary trunk banding 大鼠肺干带性右心衰模型的建立

Journal of experimental animal science Pub Date : 2007-02-26 DOI: 10.1016/j.jeas.2006.10.004

Uffe K. Schou , Christian D. Peters , Soo Wan Kim , Jørgen Frøkiær , Søren Nielsen

{"title":"Characterization of a rat model of right-sided heart failure induced by pulmonary trunk banding","authors":"Uffe K. Schou , Christian D. Peters , Soo Wan Kim , Jørgen Frøkiær , Søren Nielsen","doi":"10.1016/j.jeas.2006.10.004","DOIUrl":"10.1016/j.jeas.2006.10.004","url":null,"abstract":"<div><p><span>Animal models of disease are essential for cardiovascular research. However, animal models of right-sided heart failure are few and remain poorly characterized. The aim with this study was to establish a rat model of right-sided heart failure (HF) using pulmonary trunk banding (PTB) and subsequently to characterize the systemic and cardiac changes in this model, including protein expression of SERCA2 and </span><em>α</em><span>-sarcomeric actin. Rats underwent banding or sham operation. To evaluate the development of HF over time three groups were included in this study. They were killed 2–3, 5–7 or 16–17 weeks after operation, respectively. PTB rats showed marked hypertrophy of the right ventricle (RV). Catheterization of the RV showed a three- to four-fold increase in right ventricular systolic and diastolic pressures as well as increased d</span><em>P</em>/d<em>T</em> max and d<em>P</em>/d<em>T</em><span> min. Plasma analyses revealed increased liver enzymes in most PTB groups and post mortem examination revealed congestion of the liver as well as formation of ascites and hydrothorax in many PTB rats. Immunoblotting of the RV revealed no changes in SERCA2 or </span><em>α</em><span>-sarcomeric actin. In conclusion, PTB was an effective method to induce right-sided HF. The presence of HF was confirmed by severe signs of backward failure in conjunction with markedly elevated RV pressures and reduced RV ejection fraction (EF).</span></p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 4","pages":"Pages 237-254"},"PeriodicalIF":0.0,"publicationDate":"2007-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2006.10.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54680897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

A place preference test in the fish Nile tilapia 尼罗罗非鱼的地方偏好试验

Journal of experimental animal science Pub Date : 2006-12-12 DOI: 10.1016/j.jeas.2006.01.001

Helton Carlos Delicio, Rodrigo Egydio Barreto, Edvaldo Bento Normandes, Ana Carolina Luchiari, Ana Lúcia Marcondes

引用次数: 25

Medium-term tongue carcinogenesis assays: A comparative study between 4-nitroquinoline 1-oxide (4NQO)-induced rat and dimethylbenzanthracene (DMBA)-induced hamster carcinogenesis 中期舌癌检测:4-硝基喹啉1-氧化物(4NQO)诱导大鼠和二甲基苯并蒽(DMBA)诱导的仓鼠癌变的比较研究

Journal of experimental animal science Pub Date : 2006-12-12 DOI: 10.1016/j.jeas.2006.09.001

D. Kitakawa , L.A.G. Cabral , M.E.A. Marques , D.M.F. Salvadori , D.A. Ribeiro

{"title":"Medium-term tongue carcinogenesis assays: A comparative study between 4-nitroquinoline 1-oxide (4NQO)-induced rat and dimethylbenzanthracene (DMBA)-induced hamster carcinogenesis","authors":"D. Kitakawa , L.A.G. Cabral , M.E.A. Marques , D.M.F. Salvadori , D.A. Ribeiro","doi":"10.1016/j.jeas.2006.09.001","DOIUrl":"10.1016/j.jeas.2006.09.001","url":null,"abstract":"<div><p>The most used animal models in oral cancer research are the hamster treated by dimethylbenzanthracene (DMBA), and the rat treated by 4-nitroquinoline 1-oxide (4NQO). The purpose of this study was to compare the DMBA-induced hamster tongue carcinogenesis and 4NQO-induced rat tongue carcinogenesis by means of morphological analysis. Male Wistar rats were distributed into three groups of ten animals each and treated with 50<!--> <span>ppm 4NQO solution by drinking water for 4, 12 or 20 weeks. A total of 18 Syrian golden hamsters were submitted to 0.5% DMBA (dissolved in acetone) topical application three times/week for 4, 12 and 20 weeks. The primary histopathological change i.e., hyperplasia and hyperkeratosis<span>, was evidenced after 4 weeks treatment with DMBA. Regarding 12 weeks treatment, 4NQO and DMBA were able to induce morphological changes as depicted by hyperplasia and dysplasia. At 20 weeks, squamous cell carcinoma was found in the majority of animals for both carcinogens used. Taken together, our results suggest that the hamster experimental model disclosed aspects related with tongue carcinogenesis in lesser time than rats. Probably, such discrepancies depend strongly on route of administration and the susceptibility with respect to animal species.</span></span></p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 3","pages":"Pages 219-227"},"PeriodicalIF":0.0,"publicationDate":"2006-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2006.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54680044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Expression of a variant of human tissue-type plasminogen activator in transgenic mouse milk 人组织型纤溶酶原激活物在转基因小鼠乳汁中的表达

Journal of experimental animal science Pub Date : 2006-12-12 DOI: 10.1016/j.jeas.2006.08.002

Sha Hong-Ying , Liu Si-Guo , Chen Jian-Quan , Zhang Ai-Min , Cheng Guo-Xiang

引用次数: 5

Near-infrared imaging of flare-up arthritis with native fluorochrome Cy5.5 and albumin-bound Cy5.5 用天然荧光色素Cy5.5和白蛋白结合Cy5.5进行关节炎发作的近红外成像

Journal of experimental animal science Pub Date : 2006-12-12 DOI: 10.1016/j.jeas.2005.04.001

Andreas Hansch , Ingrid Hilger , Oliver Frey , Dieter Sauner , Mieczyslaw Gajda , Michael Haas , Ansgar Malich , Joachim Böttcher , Rolf Bräuer , Werner A. Kaiser

{"title":"Near-infrared imaging of flare-up arthritis with native fluorochrome Cy5.5 and albumin-bound Cy5.5","authors":"Andreas Hansch , Ingrid Hilger , Oliver Frey , Dieter Sauner , Mieczyslaw Gajda , Michael Haas , Ansgar Malich , Joachim Böttcher , Rolf Bräuer , Werner A. Kaiser","doi":"10.1016/j.jeas.2005.04.001","DOIUrl":"10.1016/j.jeas.2005.04.001","url":null,"abstract":"<div><p>The aim of the study was to visualize chronic experimental arthritis with near-infrared fluorescence imaging (NIRF) in a murine experimental arthritis model of rheumatoid arthritis (RA) (flare-up arthritis). The flare-up arthritis model is a modification of the primary antigen-induced arthritis (AIA) model. NIRF was done for two preparations of the fluorochrome Cy5.5, one native and the other albumin conjugated. Histological features of flare-up arthritis were evaluated.</p><p>AIA was induced in 16 mice (strain C57/Bl6); flare-up arthritis was induced in a subgroup of eight. On day 7 after induction of flare-up arthritis, four mice received 50nmol/kg native dye and four mice equimolar concentrations of the dye as albumin-dye conjugate intravenously. NIRF imaging was performed immediately before injection (baseline) and until 72h thereafter. Arthritis severity was evaluated histologically for primary AIA and flare-up arthritis mice.</p><p>NIRF imaging revealed higher fluorochrome uptake in all inflamed knees compared to contralateral ones. The signal intensities induced by native Cy5.5 were higher than those generated by albumin–Cy5.5 conjugate. Histological evaluation of arthritic joints showed similar abnormalities in flare-up arthritis and in primary AIA joints.</p><p>Imaging of flare-up arthritis in the near-infrared range was successful for both fluorochrome preparations, but albumin conjugation prior to injection does not improve the uptake of dye in arthritic joints. Flare-up arthritis is a feasible model of chronic relapse of arthritis in human RA.</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 3","pages":"Pages 129-139"},"PeriodicalIF":0.0,"publicationDate":"2006-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2005.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54679767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Microvasculature of the mouse eye: Scanning electron microscopy of vascular corrosion casts 小鼠眼睛微血管:血管腐蚀铸件的扫描电镜

Journal of experimental animal science Pub Date : 2006-12-12 DOI: 10.1016/j.jeas.2006.05.002

Hiroyoshi Ninomiya, Tomo Inomata

{"title":"Microvasculature of the mouse eye: Scanning electron microscopy of vascular corrosion casts","authors":"Hiroyoshi Ninomiya, Tomo Inomata","doi":"10.1016/j.jeas.2006.05.002","DOIUrl":"10.1016/j.jeas.2006.05.002","url":null,"abstract":"<div><h3>Purpose</h3><p>For drug safety assessment, ophthalmic fundus examination is of diagnostic importance in experimental animals. Interim blood samples are usually collected from the orbital venous sinus in the mouse. This report characterizes the angioarchitecture of the mouse eye.</p></div><div><h3>Methods</h3><p>In 10 mice, the microvasculature of the eyes of was investigated using scanning electron micrographs of corrosion casts.</p></div><div><h3>Results</h3><p><span>The mouse eye was characterized as having a rich vasculature with many thick retinal arteries and a well-developed orbital venous sinus. The eye receives its primary blood supply from the external ophthalmic artery, which is divided into three branches: the central retinal artery, as well as the medial and lateral long posterior ciliary arteries<span>. The central retinal artery is divided into 8–9 radiating retinal arteries. The mouse has an orbital venous sinus around the orbit rather than a well-developed orbital venous plexus in the retrobulbar space as is in the rat. The orbital venous sinus is formed by the episcleral veins, the </span></span>ophthalmic vein, the superior palpebral vein, inferior palpebral vein and numerous anastomotic veins among these veins.</p></div><div><h3>Conclusions</h3><p>The mouse ocular vasculature is quite similar to that of rats. It is recommended that the best location for insertion of a capillary tube for collecting blood is in the lateral canthus around the eye where the sinus is larger and is most readily accessible. Functional significance of the vascular patterns of the eye is discussed.</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 3","pages":"Pages 149-159"},"PeriodicalIF":0.0,"publicationDate":"2006-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2006.05.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54679936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Post-operative course in a surgical model of acute myocardial infarction in the rabbit 兔急性心肌梗死手术模型的术后过程

Journal of experimental animal science Pub Date : 2006-12-12 DOI: 10.1016/j.jeas.2006.08.001

P. Ypsilantis , V. Didilis , I. Bougioukas , C. Tsigalou , T. Lialiaris , C. Simopoulos , G. Bougioukas

{"title":"Post-operative course in a surgical model of acute myocardial infarction in the rabbit","authors":"P. Ypsilantis , V. Didilis , I. Bougioukas , C. Tsigalou , T. Lialiaris , C. Simopoulos , G. Bougioukas","doi":"10.1016/j.jeas.2006.08.001","DOIUrl":"10.1016/j.jeas.2006.08.001","url":null,"abstract":"<div><p><span>This study was undertaken to evaluate the post-operative (post-OP) course of rabbits in a surgical model of myocardial infarction (MI). Ten </span>New Zealand White rabbits<span><span><span> were subjected to coronary artery<span> ligation after lateral thoracotomy. Anesthesia was delivered using a xylazine–ketamine–isoflurane protocol after endotracheal intubation. Among other cardiorespiratory parameters, arterial pressure was monitored via central ear artery catheterization. Post-OP care included oxygen, antibiotic and analgesic treatment as well as nutritional and fluid support. A series of cardiorespiratory, hematological, blood biochemical and clinical parameters were assessed during the first 21 days. The mortality rate was 10%. Hypotension and hypothermia, noted during the operation, reverted within the first few post-MI hours. Electrocardiographic alterations, which included ST segment elevation and Q wave, appeared after MI and remained throughout the study period. </span></span>Tachycardia was observed during the first 5 days. Hematological examination revealed mild and transient anemia during the post-OP period and leucocytosis up to the 5th day. Urea was increased up to the 12th post-MI hour while increased </span>AST<span>, ALT, LDH and CK levels normalized by the first 4–5 days. Although the post-OP anorexia period lasted around 5 days, the animals did not manage to regain their body weight by the end of the study period. In conclusion, the present model offers a low mortality rate and could be useful in mid- or long-term MI studies. The animals go through a critical post-OP period of around 5 days during which special care should be given. After that time, their clinical and blood laboratory parameters tend to normalize.</span></span></p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 3","pages":"Pages 197-209"},"PeriodicalIF":0.0,"publicationDate":"2006-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2006.08.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54679948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The angiogenic potential of autogenous free omental graft in experimental tibial defects in rabbit: Short-term preliminary histopathological study 兔实验性胫骨缺损自体网膜游离移植血管生成潜能的短期初步组织病理学研究

Journal of experimental animal science Pub Date : 2006-12-12 DOI: 10.1016/j.jeas.2006.02.002

M.M. Oloumi , A. Derakhshanfar , M.M. Molaei , M. Tayyebi

{"title":"The angiogenic potential of autogenous free omental graft in experimental tibial defects in rabbit: Short-term preliminary histopathological study","authors":"M.M. Oloumi , A. Derakhshanfar , M.M. Molaei , M. Tayyebi","doi":"10.1016/j.jeas.2006.02.002","DOIUrl":"10.1016/j.jeas.2006.02.002","url":null,"abstract":"<div><p><span><span>This study was designed to evaluate the role of free autogenous greater omentum in </span>vasculogenesis<span> during the process of bone healing. The study was carried out on 15 male adult white New Zealand rabbits in the same condition. Both tibiae of the animals were exposed medially and a 2</span></span> <span>mm hole was drilled at proximal diaphysis. A small piece of greater omentum, obtained from a 1</span> <span>cm mid-abdominal incision was secured on left tibial hole. The animals were euthanized 14 days after operation and the bones removed for histopathological and immunohistochemical (anti-CD31 application for endothelial cell labeling) studies. In histopathological sections, the soft callus area in treatment legs was significantly more than the control legs. Also, the hole diameter in treatment group was significantly less than the control group. The number of capillary buds in treatment legs was significantly more than the control group. This study showed that an autogenous free greater omentum graft can influence bone healing through augmentation of vasculogenesis, as an essential element for proper bone healing.</span></p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 3","pages":"Pages 179-187"},"PeriodicalIF":0.0,"publicationDate":"2006-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2006.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54679869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

The incidence of giant spike bursts and repetitive bursts of action potentials in the ovine small bowel before and after cholecystokinin administration 在给药前后，绵羊小肠中巨大的脉冲和动作电位的重复爆发的发生率

Journal of experimental animal science Pub Date : 2006-12-12 DOI: 10.1016/j.jeas.2006.02.001

K.W. Romański

{"title":"The incidence of giant spike bursts and repetitive bursts of action potentials in the ovine small bowel before and after cholecystokinin administration","authors":"K.W. Romański","doi":"10.1016/j.jeas.2006.02.001","DOIUrl":"10.1016/j.jeas.2006.02.001","url":null,"abstract":"<div><p>Giant spike bursts (GSBs) or giant contractions (GCs) and repetitive bursts of action potentials (RBAPs) are less common motility patterns as compared to the migrating motor complex (MMC), fed pattern or minute rhythm. They are present in small and large intestines in various animal species. Their occurrence in ruminants has not been satisfactorily evidenced. Thus, the aim of this study was to present the incidence of these patterns in the ovine small bowel before and after different doses of cholecystokinin octapeptide (CCK-OP) and cerulein as well as to demonstrate the motor correlates of RBAPs.</p><p><span>Six sheep equipped with electrodes in the antrum and entire small intestine and with duodenal strain gauge force transducer were used. In fasted and non-fasted animals, continuous myoelectrical and motor recordings were performed before and after the slow injection of cholecystokinin octapeptide (20, 200 and 2000</span>    <!-->MMC. The incidence of GSBs and RBAPs was assessed and these patterns arrived before and after Cholecystokinin (CCK). During the control period RBAPs were most frequently observed in the ileum. GSBs and RBAPs were induced by the highest dose of the hormones. RBAPs exhibited the motor correlates and their tonic component was more pronounced following CCK-OP and cerulein injection.</p><p>It is concluded that GSBs and RBAPs occur in the small intestine and the administration of CCK peptides further increases their incidence.</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 3","pages":"Pages 161-178"},"PeriodicalIF":0.0,"publicationDate":"2006-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2006.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54679841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Ventilatory frequency of Nile tilapia subjected to different stressors 不同应激源对尼罗罗非鱼通气频率的影响

Journal of experimental animal science Pub Date : 2006-12-12 DOI: 10.1016/j.jeas.2006.05.001

Rodrigo Egydio Barreto, Gilson Luiz Volpato

{"title":"Ventilatory frequency of Nile tilapia subjected to different stressors","authors":"Rodrigo Egydio Barreto, Gilson Luiz Volpato","doi":"10.1016/j.jeas.2006.05.001","DOIUrl":"10.1016/j.jeas.2006.05.001","url":null,"abstract":"<div><p>Ventilatory frequency (VF) was investigated in the fish Nile tilapia, <span><em>Oreochromis niloticus</em></span>, subjected to confinement, electroshock or social stressor. Fish were allowed to acclimatize to tank conditions for 10 days (1 fish/aquarium). VF baseline was determined 5 days after adjustment had been started. At the 10th day of isolation, stressor effects on VF were assessed. The stressors were imposed during 60min: pairing with a larger resident animal (social stressor), or gentle electroshock (AC, 20V, 15mA, 100Hz for 1min every 4min), or space restriction (confinement). VF was quantified immediately after the end of the stressor imposition. Baseline of VF was not statistically different among groups. Social stressor clearly induced VF to increase, while an increased or decreased VF can be observed for both confinement and electroshock. However, fish tend to increase their VF in response to confinement and decrease in the case of electroshock. These results suggest that VF is a sensitive behavioural indicator for distinguishing stress responses in the fish Nile tilapia among different stressors.</p></div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 3","pages":"Pages 189-196"},"PeriodicalIF":0.0,"publicationDate":"2006-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2006.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54679920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40