Journal of experimental animal science最新文献_第3页

Quantification of MyHC mRNA in masticatory muscles during orthodontic treatment in pigs 正畸治疗期间猪咀嚼肌MyHC mRNA的定量分析

Journal of experimental animal science Pub Date : 2005-05-26 DOI: 10.1016/j.jeas.2004.11.002

Olaf Luck , Cordula Büttner , Tomas Gedrange , Torsten Kotyra , Winfried Harzer

{"title":"Quantification of MyHC mRNA in masticatory muscles during orthodontic treatment in pigs","authors":"Olaf Luck , Cordula Büttner , Tomas Gedrange , Torsten Kotyra , Winfried Harzer","doi":"10.1016/j.jeas.2004.11.002","DOIUrl":"10.1016/j.jeas.2004.11.002","url":null,"abstract":"<div>Orthodontic treatment of retrognathism is aimed at correcting the position of the jaws and at rectifying functional problems. Changes induced in the bone morphology of the mandible and in the dentoalveolar region by different appliances are well documented. Less is known and measurable with respect to the functional status and muscular capacity of orofacial muscles induced by the appliance within the framework of the training effect in Class II malocclusion cases. The aim of the present investigation was to analyze and quantify the change in fiber type levels of type I (slow Myosin heavy chain (MyHC)) and type IIb (fast b MyHC) MyHC RNA transcripts, using the competitive reverse polymerase chain reaction (cRT-PCR) during sagittal advancement of the mandible in pigs. The experiments were carried out on 10-week-old piglets (six test animals, six controls) over a period of 28 days. Six piglets received acrylic build-ups for permanent sagittal advancement of the mandible. Muscle tissue was taken from seven different regions of the masseter (m1-3), temporal (tp1, 2), medial pterygoid (pm), and geniohyoid muscles (gh). A marked increase in the absolute amount of slow MyHC RNA transcripts was detected after treatment in the anterior region and in the medial region of the masseter muscle, and in the anterior region of the temporal muscle. For fast b MyHC fibers no significant changes were registered. This can be seen as a shift directed to slow MyHC, confirming results of the training effect in sport and rehabilitation medicine.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 2","pages":"Pages 101-115"},"PeriodicalIF":0.0,"publicationDate":"2005-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2004.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54679569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Morphometric changes in the adrenal medulla of intact and ethane dimethanesulphonate (EDS)-injected rats subjected to chronic treatment with isoproterenol or propranolol 异丙肾上腺素或心得安慢性治疗大鼠肾上腺髓质的形态学变化

Journal of experimental animal science Pub Date : 2005-05-26 DOI: 10.1016/j.jeas.2005.02.001

Bosiljka Plećaš-Solarović, Gordana Leposavić

{"title":"Morphometric changes in the adrenal medulla of intact and ethane dimethanesulphonate (EDS)-injected rats subjected to chronic treatment with isoproterenol or propranolol","authors":"Bosiljka Plećaš-Solarović, Gordana Leposavić","doi":"10.1016/j.jeas.2005.02.001","DOIUrl":"10.1016/j.jeas.2005.02.001","url":null,"abstract":"<div>Morphometric characteristics of adrenal medulla were analysed stereologically in adult male rats injected with a single dose of ethane dimethanesulphonate (EDS), an agent that causes atrophy of the inner adrenocortical zone, or vehicle, and subjected to isoproterenol (ISO) or propranolol (PROP) treatment over the following 15 days. Compared with dimethylsulphoxide (DMSO) vehicle-injected controls, in EDS-administered rats the volume of chromaffin cell nuclei was decreased. ISO treatment in these rats increased the volumes of chromaffin cells and their nuclei. Furthermore, in both EDS- and vehicle-injected rats ISO significantly enlarged the total volume of medullary blood vessels, suggesting a vasodilatatory effect of <math><mrow><mi>β</mi></mrow></math>-adrenoceptor stimulation. However, unexpectedly, in EDS-injected rats PROP treatment also caused an increase in the volumes of chromaffin cells and their nuclei. This finding, most likely, may be related to a non-<math><mrow><mi>β</mi></mrow></math>-adrenoceptor-related action of PROP. Collectively, the present results suggest that the response of adrenomedullary chromaffin cells to chronic ISO-induced <math><mrow><mi>β</mi></mrow></math>-adrenoceptor stimulation is dependent on the functional status of adrenal cortex, so that the stereologically detectable changes were found only in rats previously exposed to the destructive action of EDS on the adrenal gland cortical cells. Moreover, they indicate that ISO treatment exerts a reversing effect on the morphometrical changes of chromaffine cells induced by EDS administration.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 2","pages":"Pages 117-125"},"PeriodicalIF":0.0,"publicationDate":"2005-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2005.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54679657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postnatal growth in male Dunkin–Hartley guinea pigs (Cavia cutleri f. porcellus) 雄性Dunkin-Hartley豚鼠(Cavia cutleri f. porcellus)的产后生长

Journal of experimental animal science Pub Date : 2005-05-26 DOI: 10.1016/j.jeas.2004.11.001

A. Gericke, U. Gille, T. Trautvetter, F.-V. Salomon

引用次数: 4

Cryopreservation of transgenic mouse embryos — an eight years’ experience 转基因小鼠胚胎的低温保存——八年的经验

Journal of experimental animal science Pub Date : 2005-05-26 DOI: 10.1016/j.jeas.2004.09.001

Klaus Wayss , Michaela Klefenz , Johannes Schenkel

引用次数: 18

第三版，Robert Pies Schulz Hofen。Parey Verlag，斯图加特（2004），（138,00欧元），国际标准图书编号：3-8304-4134-7

Journal of experimental animal science Pub Date : 2005-05-26 DOI: 10.1016/j.jeas.2005.02.002

M.M. Dorsch

引用次数: 0

A new transplant bone for maxillary alveolar cleft 上颌牙槽嵴裂的新移植骨

Journal of experimental animal science Pub Date : 2004-04-30 DOI: 10.1016/j.jeas.2004.01.004

T Kawata , A Matsuki , S Kohno , T Fujita , H Sugiyama , C Tokimasa , M Kaku , K Tsutsui , H.B Moon , K Tanne

{"title":"A new transplant bone for maxillary alveolar cleft","authors":"T Kawata , A Matsuki , S Kohno , T Fujita , H Sugiyama , C Tokimasa , M Kaku , K Tsutsui , H.B Moon , K Tanne","doi":"10.1016/j.jeas.2004.01.004","DOIUrl":"10.1016/j.jeas.2004.01.004","url":null,"abstract":"<div>An innovative technique with distraction osteogenesis has been developed in our research group to explore autogenous bone transplantation into craniofacial bone defects. This technique was designed to investigate bone-marrow transplantion using a chondroid or fibula bone graft into simulated alveolar bone defects in mice in terms of the osteogenic process and activity. As an experimental model of maxillary alveolar bone cleft available for testing bone-inductive materials, a critical-size defect was formed in the pre-maxillary bone of male mice using a surgical trephine bur with a low-speed dental engine. Distraction osteogenesis was performed using an external fixation device. The osteotomy site was occupied by an external callus consisting of hyaline cartilage with a large quantity of chondroid bone. Moreover, bone remodelling with new bone formation was demonstrated 30 days after the transplantation. Bone adhesion was better in chondroid bone grafting than in fibula bone grafting. The present findings are the first to demonstrate the potential of chondroid bone transplantation as a new therapeutic system of bone grafting, suitable for bone substitutes in craniofacial bone defects.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 1","pages":"Pages 19-28"},"PeriodicalIF":0.0,"publicationDate":"2004-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2004.01.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54679281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Problems in physiological experimental animal models investigated with factorial design 用因子设计研究生理实验动物模型中的问题

Journal of experimental animal science Pub Date : 2004-04-30 DOI: 10.1016/j.jeas.2004.01.002

Eli-Anne B Gjerde , Dag Marcus Eide , Aurora Brønstad , Rolf K Reed

{"title":"Problems in physiological experimental animal models investigated with factorial design","authors":"Eli-Anne B Gjerde , Dag Marcus Eide , Aurora Brønstad , Rolf K Reed","doi":"10.1016/j.jeas.2004.01.002","DOIUrl":"10.1016/j.jeas.2004.01.002","url":null,"abstract":"<div>In the present study we investigated four variables using factorial design to decide if any of these could explain the variations in the control measurements of interstitial fluid pressure (Pif) in rat trachea that were experienced. This approach requires only a fraction of the animals normally needed when studying each factor separately. Pif in tracheal tissue was measured with the servocontrolled counterpressure system using sharpened micropipettes. The measurements were performed over a period of 60min and are presented as mean for every 15min period. The factors investigated in the study were: three strains of female rats (Strain) two brands of diets (Food); two breeder companies (Source); and finally two batches of the same set of animals to repeat the experiment twice (Week), using a total of 48 animals. There was a highly significant effect within Strain the first week (p=0.007), but this response was not observed the second week. The interaction between Strain×Week was significant (p=0.007) while the main effects Strain or Week alone were not significant. The response pattern for Strain and Food was inconsistent for the two experimental weeks studied. These experiments made it possible for us to simultaneously test several factors and exclude these factors as the reason for the observed changes in our experiments since the experiments did not allow the conclusion that one or several of these factors could explain the variation in Pif.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 1","pages":"Pages 1-12"},"PeriodicalIF":0.0,"publicationDate":"2004-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2004.01.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37833307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Spontaneous tubular (cuboidal) metaplasia of the parietal layer of Bowman's capsule in Cynomolgus monkeys (Macaca fascicularis) 食蟹猴(Macaca fascularis)鲍曼囊壁层自发管状(立方)化生

Journal of experimental animal science Pub Date : 2004-04-30 DOI: 10.1016/j.jeas.2004.01.001

J Kaspareit, S Friderichs-Gromoll, E Buse, G Habermann

引用次数: 5

Inherited copper toxicosis with emphasis on copper toxicosis in Bedlington terriers 遗传铜中毒，重点在贝德灵顿梗铜中毒

Journal of experimental animal science Pub Date : 2004-04-30 DOI: 10.1016/j.jeas.2004.01.003

Changbaig Hyun , Lucio J Filippich

引用次数: 9

Penetration and maturation of Schistosoma mansoni in suckling and adult Swiss Webster and DBA/2 mice 曼氏血吸虫在哺乳期和成年瑞士韦伯斯特小鼠和DBA/2小鼠体内的渗透和成熟

Journal of experimental animal science Pub Date : 2004-04-30 DOI: 10.1016/j.jeas.2004.02.001

A.A Fidalgo-Neto, R.R De-Carvalho, A.C.A.X De-Oliveira, D.A Manhães-Rocha, F.J.R Paumgartten

{"title":"Penetration and maturation of Schistosoma mansoni in suckling and adult Swiss Webster and DBA/2 mice","authors":"A.A Fidalgo-Neto, R.R De-Carvalho, A.C.A.X De-Oliveira, D.A Manhães-Rocha, F.J.R Paumgartten","doi":"10.1016/j.jeas.2004.02.001","DOIUrl":"10.1016/j.jeas.2004.02.001","url":null,"abstract":"<div>Murine schistosomosis is a widely used experimental model of the human disease. Different methods have been employed to infect mice with Schistosoma mansoni cercariae, such as subcutaneous or intraperitoneal injections, the tail immersion technique, and the use of a metal ring placed on the abdominal skin of anaesthetized animals. An alternative method of infection that requires no anaesthesia and no restraint is to place suckling mice (10 days old) on a Petri dish with a small volume of water containing cercariae. In this study, we compared the penetration and maturation of S. mansoni in mice infected by this alternative method with those noted in mice infected at an older age (45 days) by the tail immersion technique. Besides evaluating the effects of age and method of infection, we also compared the susceptibility of two strains of mice (Swiss Webster (SW) and DBA/2). Mice were exposed to 100 cercariae and worms (by portal–hepatic perfusion) as well as eggs were recovered in the liver and intestines on postinfection (PI) days 35, 55 and 90. Skin penetration was very efficient (about 100%) irrespective of the mouse strain, sex, age and method of infection. Worm and egg recoveries were higher in SW mice at any PI interval, but strain differences tended to be less pronounced on PI day 90. In both strains, recoveries of worms and eggs were clearly higher in mice infected at a younger age (10 days old). This study thus suggests that infection of free-moving suckling mice is a suitable alternative to other methods of infection with S. mansoni.</div>","PeriodicalId":77206,"journal":{"name":"Journal of experimental animal science","volume":"43 1","pages":"Pages 29-38"},"PeriodicalIF":0.0,"publicationDate":"2004-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jeas.2004.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54679313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4