The angiogenic potential of autogenous free omental graft in experimental tibial defects in rabbit: Short-term preliminary histopathological study

Journal of experimental animal science Pub Date : 2006-12-12 DOI:10.1016/j.jeas.2006.02.002

M.M. Oloumi , A. Derakhshanfar , M.M. Molaei , M. Tayyebi

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引用次数: 25

Abstract

This study was designed to evaluate the role of free autogenous greater omentum in vasculogenesis during the process of bone healing. The study was carried out on 15 male adult white New Zealand rabbits in the same condition. Both tibiae of the animals were exposed medially and a 2 mm hole was drilled at proximal diaphysis. A small piece of greater omentum, obtained from a 1 cm mid-abdominal incision was secured on left tibial hole. The animals were euthanized 14 days after operation and the bones removed for histopathological and immunohistochemical (anti-CD31 application for endothelial cell labeling) studies. In histopathological sections, the soft callus area in treatment legs was significantly more than the control legs. Also, the hole diameter in treatment group was significantly less than the control group. The number of capillary buds in treatment legs was significantly more than the control group. This study showed that an autogenous free greater omentum graft can influence bone healing through augmentation of vasculogenesis, as an essential element for proper bone healing.

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兔实验性胫骨缺损自体网膜游离移植血管生成潜能的短期初步组织病理学研究

本研究旨在评估骨愈合过程中游离自体大网膜在血管生成中的作用。这项研究是在15只成年雄性新西兰白兔身上进行的。动物的双胫骨向内侧暴露，在骨干近端钻孔2mm。从腹部正中切口取一小片大网膜固定在左侧胫骨孔上。术后14天对动物实施安乐死，取骨进行组织病理学和免疫组化(抗cd31应用于内皮细胞标记)研究。在组织病理切片上，实验组的软愈伤组织面积明显大于对照组。治疗组的孔直径明显小于对照组。处理腿毛细血管芽数明显多于对照组。本研究表明，自体游离大网膜移植物可以通过增强血管生成来影响骨愈合，这是骨愈合的必要因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of experimental animal science

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