Journal of biological standardization最新文献_第5页

The use of a nitrocellulose-enzyme immunoassay for the rapid screening of monoclonal antibodies to human enteroviruses 利用硝化纤维素酶免疫分析法快速筛选人肠道病毒单克隆抗体

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/0092-1157(89)90022-X

F. Fuchs-Beraud , M. Aymard

引用次数: 2

The quality control of tuberculin PPD products from seven Latin American laboratories 七个拉丁美洲实验室结核菌素PPD产品的质量控制

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/0092-1157(89)90015-2

Isabel N. de Kantor , Graciela Spizzamiglio , Alejandro Costa , Vicente Garcia

{"title":"The quality control of tuberculin PPD products from seven Latin American laboratories","authors":"Isabel N. de Kantor , Graciela Spizzamiglio , Alejandro Costa , Vicente Garcia","doi":"10.1016/0092-1157(89)90015-2","DOIUrl":"10.1016/0092-1157(89)90015-2","url":null,"abstract":"<div><p>The Pan American Health Organization, through its Pan American Zoonoses Center (CEPANZO), distributes the concentrated stock solution of PPD batch RT23 (1 mg ml<sup>−1</sup> = 50 000 TU ml<sup>−1</sup>) to national central laboratories. In the laboratories the stock solution is diluted to 2 TU/0·1 ml and dispensed with the addition of Tween 80 and either chinosol or phenol as preservatives in rubber-stoppered vials for use in the Mantoux test. The expiry date is usually fixed at six months after filling. Samples of nine lots of PPD 2 TU from seven countries (Argentina, Brazil, Colombia, Dominican Republic, Ecuador, Paraguay and Uruguay) were received by CEPANZO for quality control. The biological activity and phenol concentrations of these samples were assayed periodically after storage at 4, 25 or 37 °C either unopened or opened and re-used in a manner simulating extreme field conditions of use and storage. The object of the study was to determine the stability of the biological activity as well as the maintenance of sterility in these conditions with a view to assessing the need for stricter requirements for the labelling of tuberculin.</p><p>The results obtained showed that PPD 2 TU with 0·4–0·5% (<span><math><mtext>w</mtext><mtext>v</mtext></math></span>) phenol added, and dispensed in vials, was stable in its biological activity and maintained its sterility. Therefore, it seems unnecessary to issue stricter requirements than those currently applied. PPD 2 TU can be used for six months after dilution provided that it has been stored at 5 ± 3 °C, protected from sunlight, and held in vials or ampoules filled to capacity provided that containers used twice with a remaining volume of half or less of the original volume were discarded.</p></div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"17 3","pages":"Pages 233-239"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(89)90015-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13933358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Etude des anatoxines diphtériques et tétaniques par électrofocalisation en gel d’agarose 琼脂糖凝胶电聚焦白喉和破伤风安纳托辛的研究

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/S0092-1157(89)80005-8

Jacques Bourleaud

引用次数: 0

The effects of purified pertussis components and lipopolysaccharide on the results of the mouse weight gain test 纯化百日咳成分及脂多糖对小鼠增重试验结果的影响

Journal of biological standardization Pub Date : 1988-01-01 DOI: 10.1016/0092-1157(88)90020-0

R.K. Gupta , S.N. Saxena , Suniti B. Sharma , Subhash Ahuja

{"title":"The effects of purified pertussis components and lipopolysaccharide on the results of the mouse weight gain test","authors":"R.K. Gupta , S.N. Saxena , Suniti B. Sharma , Subhash Ahuja","doi":"10.1016/0092-1157(88)90020-0","DOIUrl":"10.1016/0092-1157(88)90020-0","url":null,"abstract":"<div><p>The effects of highly purified components of <em>Bordetella pertussis</em>, that is pertussis toxin (PT) and filamentous haemagglutinin (FHA), and of lipopolysaccharide (LPS) were studied in the active mouse weight gain test (MWGT). The PT when given alone or with other components in various combinations caused weight losses and deaths 2–3 days after inoculation but FHA was not toxic in the MWGT. When FHA was given with PT, the toxic effect of PT was reduced. The LPS caused weight losses at 24 h which decreased when LPS was given with PT. The toxic effects of PT as indicated by late deaths and late weight losses or failure to gain weight continued until 14 days after inoculation. The various components had similar effects on mouse weight gain in both LACA and NIH strains of mice. The doses of PT used in the MWGT caused marked leucocytosis but FHA and LPS did not. No agglutinins appeared in the sera of mice inoculated with various purified components. The components were thus pure and did not contain agglutinogens.</p></div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"16 4","pages":"Pages 321-331"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(88)90020-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14328492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

The production and evaluation of monoclonal antibodies to Clostridium perfringens type D epsilon toxin 产气荚膜梭菌D型epsilon毒素单克隆抗体的制备及评价

Journal of biological standardization Pub Date : 1988-01-01 DOI: 10.1016/0092-1157(88)90008-X

C.D.H. Boarer , M.G. Sojka , V.J. White , P.L. Roeder

引用次数: 12

Lipopolysaccharides in a traditional pertussis vaccine 传统百日咳疫苗中的脂多糖

Journal of biological standardization Pub Date : 1988-01-01 DOI: 10.1016/0092-1157(88)90018-2

P. Ibsen , S. Møller , I. Heron

{"title":"Lipopolysaccharides in a traditional pertussis vaccine","authors":"P. Ibsen , S. Møller , I. Heron","doi":"10.1016/0092-1157(88)90018-2","DOIUrl":"10.1016/0092-1157(88)90018-2","url":null,"abstract":"<div><p>Analysis of the lipopolysaccharide (LPS, endotoxin) in cell sonicates of four Danish vaccine strains of <em>Bordetella pertussis</em> (3803, 3825, 3843 and 3860) and of purified strain 3803 LPS in sodium dodecyl sulphate-polyacrylamide gel electrophoresis by silver staining, showed identical profiles. The LPS profile revealed a dominant, brownish LPS II band and a minor, faster-migrating, black-stained LPS I band. However, the ratio of LPS I to LPS II in the preparation of purified LPS differed slightly from the cell sonicates. Using marker LPS, the molecular weights of LPS I and LPS II were estimated at 5·4 and 6·0 kD, respectively. Seven different lots of whole cell <em>pertussis</em> vaccine were assayed for LPS in the <em>Limulus</em> Amoebocyte Lysate test and were found to contain 0·9 – 2·8 μg LPS/ml. No significant difference in the content of LPS in similar dilutions of the individual strains was observed. In addition, the distribution of free and cell-bound LPS in four pertussis vaccines was investigated. Most of the LPS was found to exist as free LPS. During several months, the course of both LPS and pertussis toxin (Pt) release in freshly killed <em>B. pertussis</em> preparations was followed. In the first few weeks, 35–50% of the LPS was released and after 5–6 months of storage 60–80% had been released. In contrast, less than 10% of the biologically active pertussis toxin was released during the experimental period. The possibility of producing a safer whole cell <em>pertussis</em> vaccine by reducing the amount of free LPS without reducing the protective value correspondingly is discussed.</p></div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"16 4","pages":"Pages 299-302, IN5-IN6, 303-309"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(88)90018-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14040269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

The toxin binding inhibition test as a reliable in vitro alternative to the toxin neutralization test in mice for the estimation of tetanus antitoxin in human sera 毒素结合抑制试验是测定人血清中破伤风抗毒素的可靠的体外替代毒素中和试验方法

Journal of biological standardization Pub Date : 1988-01-01 DOI: 10.1016/0092-1157(88)90017-0

C.F.M. Hendriksen , J.W.v.d. Gun , J. Nagel , J.G. Kreeftenberg

{"title":"The toxin binding inhibition test as a reliable in vitro alternative to the toxin neutralization test in mice for the estimation of tetanus antitoxin in human sera","authors":"C.F.M. Hendriksen , J.W.v.d. Gun , J. Nagel , J.G. Kreeftenberg","doi":"10.1016/0092-1157(88)90017-0","DOIUrl":"10.1016/0092-1157(88)90017-0","url":null,"abstract":"<div><p>A method for the screening of human sera for tetanus antibodies has been developed and evaluated. The toxin binding inhibition test (ToBI-test) is based on inhibition of the binding of tetanus toxin to an antitoxin-coated immunoassay microtitre plate by tetanus antibodies. Serum samples from 191 healthy adults with different vaccination histories have been titrated for tetanus antibodies by the toxin neutralization (TN) test in mice, by toxoid-ELISA and by the ToBI-test. In every respect, the ToBI-test proved to be the best <em>in vitro</em> alternative to the TN-test in mice. Comparisons showed a higher degree of correlation between the ToBI-test and the TN-test than between the toxoid-ELISA and the TN-test. Furthermore, no overestimation of antibody content was seen in titrating low titre sera by the ToBI-test. In contrast, several false positive results were seen when using the toxoid-ELISA. It is concluded that the ToBI-test is a reliable and precise alternative to the TN-test and can be performed under simple laboratory conditions in a short time.</p></div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"16 4","pages":"Pages 287-297"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(88)90017-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14328490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 84

Methode de titrage de Chlamydia psittaci par immunofluorescence 免疫荧光法测定鹦鹉热衣原体的方法。

Journal of biological standardization Pub Date : 1988-01-01 DOI: 10.1016/0092-1157(88)90009-1

Vincent Fuentes , François Eb , Jeanne Orfila

引用次数: 4

A test for the relative potency of herpes simplex virus vaccines based upon the female guinea-pig model of HSV 2 genital infection 基于HSV 2型雌性豚鼠生殖器感染模型的单纯疱疹病毒疫苗相对效力测试

Journal of biological standardization Pub Date : 1988-01-01 DOI: 10.1016/0092-1157(88)90038-8

R.J. Phillpotts , M.J. Welch , P.H. Ridgeway , A.C. Walkland , J. Melling

引用次数: 14

Etude de la stabilité d'un nouveau vaccin amaril 17D thermostable 新型热稳定性amaril 17D疫苗的稳定性研究

Journal of biological standardization Pub Date : 1988-01-01 DOI: 10.1016/0092-1157(88)90023-6

J.P. Durand , B. Kollo , M. Merlin , J.Y. Le Hesran , R. Josse , G.P. Garrigue

{"title":"Etude de la stabilité d'un nouveau vaccin amaril 17D thermostable","authors":"J.P. Durand , B. Kollo , M. Merlin , J.Y. Le Hesran , R. Josse , G.P. Garrigue","doi":"10.1016/0092-1157(88)90023-6","DOIUrl":"10.1016/0092-1157(88)90023-6","url":null,"abstract":"<div><p>Une étude comparative a été menée au Nord Cameroun avec l'objectif d'évaluer l'immunogénicité d'un nouveau vaccin amaril 17D thermostable lorsque la forme reconstituée est conservée à température ambiante. Des enfants en bonne santé, âgés de six à huit ans, ont été répartis en trois groupes A, B et C par randomisation. Ils ont reçu la même dose de vaccin dont la forme reconstituée avait été conservée à une température comprise entre +25°C et +30°C pendant respectivement une heure (A), deux heures (B) et trois heures (C). La réponse sérologique a étéévaluée deux mois après vaccination chez les enfants initialement séronégatifs. 100% des enfants du groupe A, 82% du groupe B et 67% du groupe C ont produit des anticorps neutralisants dont la moyenne géométrique du titre—inverse de la dilution—est respectivement 1,589, 1,432 et 1,276. Les taux de séroconversion et les moyennes géométriques sont significativement différentes, et leur décroissance est une fonction linéaire du temps écoulé entre la reconstitution et l'administration. Aucun effet indésirable n'a été rapporté pendant l'essai.</p><p>Cette étude démontre que la forme reconstituée de ce nouveau vaccin amaril 17D thermostable garde son efficacité après conservation à température ambiante pendant deux heures.</p></div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"16 1","pages":"Pages 1-7"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(88)90023-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14477779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5