Journal of biological standardization最新文献_第4页

A collaborative study to establish the international reference reagent for hepatitis B vaccine containing plasma-derived hepatitis B surface antigen 建立含血浆源性乙型肝炎表面抗原的乙型肝炎疫苗国际参比试剂的合作研究

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/0092-1157(89)90005-X

Morag Ferguson , Valerie Seagroatt , Geoffrey C. Schild

引用次数: 2

Potency assay of antibodies against rabies. A report on a collaborative study 抗狂犬病抗体效价测定。关于合作研究的报告

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/0092-1157(89)90019-X

Jørn Lyng , M.Weis Bentzon , E.A. Fitzgerald

引用次数: 6

Notes to applicants for marketing authorizations on the production and quality control of medicinal products derived by recombinant DNA technology 关于重组DNA技术衍生药品生产和质量控制的上市许可申请人须知

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/0092-1157(89)90014-0

Committee for Proprietary Medicinal Products: Ad Hoc Working Party on Biotechnology/Pharmacy

引用次数: 0

Purification and sequencing of glycosylation variants of BSF-1, as a MAF, from the EL-4 leukaemia cell line EL-4白血病细胞系BSF-1作为MAF的糖基化变体的纯化和测序

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/0092-1157(89)90029-2

C. Sutton , P. Depledge , L. Bawden , A. Carne , M. Meltzer , V. Newton , L. Vodinelich

{"title":"Purification and sequencing of glycosylation variants of BSF-1, as a MAF, from the EL-4 leukaemia cell line","authors":"C. Sutton , P. Depledge , L. Bawden , A. Carne , M. Meltzer , V. Newton , L. Vodinelich","doi":"10.1016/0092-1157(89)90029-2","DOIUrl":"10.1016/0092-1157(89)90029-2","url":null,"abstract":"<div>Macrophage activation activity was characterized from a PMA-induced subclone of the murine EL-4 leukaemic cell line. The MAF was purified from the cell line culture supernatant by concentration, CM-Sepharose and lentil lectin Sepharose chromatography, AcA 54 gel filtration, Mono Q FPLC and reverse phase HPLC. Four protein bands of different abundance were observed on SDS-PAGE with molecular weights of 17 500 to 21 000 Da. Three of the four proteins were sequenced from the N-terminal and shared homology with the published sequence of BSF-1. Variation of the molecular weight due to glycosylation was demonstrated by N-glycanase treatment, all four proteins gave a band of 14 200 Da after deglycosylation. Both glycosylated and deglycosylated forms of BSF-1 were equally active in the MAF assay. A monoclonal antibody to BSF-1 neutralized 80% of the activity from crude culture supernatants in the MAF assay. These studies have indicated that BSF-1 is the major, if not the only, MAF activity from this particular subline of the murine EL-4 leukaemic cell line.</div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"17 1","pages":"Pages 65-70, IN3-IN4, 71-74"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(89)90029-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13788556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Combined estimation of tetanus and diphtheria antitoxin in human sera by the in vitro Toxin-Binding Inhibition (ToBI) test 用体外毒素结合抑制(ToBI)试验联合测定人血清中破伤风和白喉抗毒素

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/0092-1157(89)90009-7

C.F.M. Hendriksen , J.W. van der Gun , J.G. Kreeftenberg

{"title":"Combined estimation of tetanus and diphtheria antitoxin in human sera by the in vitro Toxin-Binding Inhibition (ToBI) test","authors":"C.F.M. Hendriksen , J.W. van der Gun , J.G. Kreeftenberg","doi":"10.1016/0092-1157(89)90009-7","DOIUrl":"10.1016/0092-1157(89)90009-7","url":null,"abstract":"<div>The use of the principle of inhibition of toxin binding to an antitoxin coated immunoassay plate as described in a previous paper for tetanus antitoxin titration, was adapted for the estimation of diphtheria antitoxin in human sera. With a few modifications, a Toxin-Binding Inhibition (ToBI) test was developed which could be used for a combined estimation of both tetanus and diphtheria antitoxin levels. The application of streptavidin-biotinylated peroxidase complex when using small serum samples (<50 μl) is discussed. Antitoxin titres (both diphtheria and tetanus) of 0·002 IU ml−1 were detectable by the ToBI test, this being far below the level considered to be protective in man.Sera from 140 adults with different vaccination histories were titrated for both tetanus and diphtheria antitoxin. Good correlations were found between the estimates obtained by the ToBI test and those obtained by the toxin-neutralization (TN) test in mice (tetanus antitoxin) and those obtained in the in vitro neutralization test in VERO cells (diphtheria antitoxin).It is concluded that the ToBI test is a simple and reliable alternative to the functional models currently in use for the estimation of diphtheria and tetanus antitoxin levels. In addition, the ToBI test eliminates the need for laboratory-animal or cell-culture facilities and can be performed with small quantities of serum as required in field trials.</div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"17 2","pages":"Pages 191-200"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(89)90009-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13855211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 54

Heat inactivation of human immunodeficiency virus type 2 (HIV-2) 热灭活人类免疫缺陷病毒2型(HIV-2)

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/S0092-1157(89)80009-5

J.P. Gregersen , J. Hilfenhaus , J.F. Lemp Jr

引用次数: 11

The effect of cyclodextrin on lipopolysaccharide production in cultures of Bordetella pertussis 环糊精对百日咳杆菌脂多糖产生的影响

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/S0092-1157(89)80003-4

P. Ibsen , C. Schou , M. Au-Jensen , I. Heron

{"title":"The effect of cyclodextrin on lipopolysaccharide production in cultures of Bordetella pertussis","authors":"P. Ibsen , C. Schou , M. Au-Jensen , I. Heron","doi":"10.1016/S0092-1157(89)80003-4","DOIUrl":"10.1016/S0092-1157(89)80003-4","url":null,"abstract":"<div>The effect of adding 500 μg of (2,6-0-dimethyl) β-cydodexcrin (Me-β-CD) per ml of Stainer-Scholte (SS) medium in two-day shaker flask cultures of Bordetella pertussis on the production of lipopolysaccharide (LPS) was investigated. The amount of LPS per 109 cells found in the supernatants of these cultures was either somewhat reduced or unaffected by comparison with the amounts in cultures grown in SS-medium alone. In addition, the time course of LPS release from cultures of B. pertussis strain 3843 cells during a 96-h growth period in normal and Me-β-CD-enriched SS medium is described. By using the enriched medium bacterial growth, the production of filamentous haemagglutinin (FHA) and of pertussis toxin (Pt) and the levels of haemagglutination and lymphocytosis-promoting activity were enhanced to various degrees. Measurements made on sedimented whole and on sonicated B. pertussis cells grown in the two media showed no differences in LPS content.The reasons for the reduced/unaffected LPS production are discussed. It has been suggested that an interaction between hydrophobic cavities of the Me-β-CD molecules and the ‘lipid A’ part of LPS reduces the reactivity of LPS in the Limulus Amoebocyte Lysate (LAL) assay. This possibility, however, was rejected as the reactivity of Me-β-CD-spiked purified B. pertussis strain 3803 LPS, compared with unspiked samples, remained unchanged.</div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"17 4","pages":"Pages 321-330"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0092-1157(89)80003-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13703360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Salmonella typhi vaccine strain in vitro; low infectivity in human cell line U937 伤寒沙门氏菌体外疫苗株;对人细胞系U937具有低传染性

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/S0092-1157(89)80006-X

Evgenia M. Dragunsky , Carole R. Wooden , Susan A. Vargo , Inessa S. Levenbook

引用次数: 11

Effect of strain differences on the potency testing of rabies vaccines in mice 毒株差异对狂犬疫苗效价测定的影响

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/0092-1157(89)90018-8

J. Blancou , M.F.A. Aubert , Eveline Cain , Myriam Selve , O. Thraenhart , L. Bruckner

引用次数: 19

Immunity to diphtheria, six to 15 years after a basic three-dose immunization schedule 在基本三剂免疫计划后6至15年对白喉免疫

Journal of biological standardization Pub Date : 1989-01-01 DOI: 10.1016/0092-1157(89)90025-5

Carla Cellesi , Clara Michelangeli , Gian Maria Rossolini , Franco Giovannoni , Aldo Rossolini

引用次数: 8