Journal of biological standardization最新文献_第10页

An evaluation of some proposals for a reduction in the number of animals used for the potency testing of diphtheria and tetanus vaccines 关于减少用于白喉和破伤风疫苗效力试验的动物数量的一些建议的评价

Journal of biological standardization Pub Date : 1987-01-01 DOI: 10.1016/0092-1157(87)90039-4

P.A. Knight , P.A.G. Roberts

引用次数: 7

The detection of antibodies to recombinant interferon alfa-2a in human serum 人血清中重组干扰素α -2a抗体的检测

Journal of biological standardization Pub Date : 1987-01-01 DOI: 10.1016/0092-1157(87)90026-6

U. Hennes , W. Jucker , E.A. Fischer , Th. Krummenacher , A.V. Palleroni , P.W. Trown , S. Linder-Ciccolunghi , M. Rainisio

{"title":"The detection of antibodies to recombinant interferon alfa-2a in human serum","authors":"U. Hennes , W. Jucker , E.A. Fischer , Th. Krummenacher , A.V. Palleroni , P.W. Trown , S. Linder-Ciccolunghi , M. Rainisio","doi":"10.1016/0092-1157(87)90026-6","DOIUrl":"10.1016/0092-1157(87)90026-6","url":null,"abstract":"<div>Three different procedures have been used for detecting antibodies to Roferon-A (recombinant human interferon alfa-2a, rHuIFNα-2a) in the serum of patients who received this interferon as part of ongoing clinical trials: an antiviral neutralization bioassay (ANB), the standard method recommended by the World Health Organization (WHO), and the more recently developed radioimmunoassay (RIA) and enzymeimmunoassay (EIA). Although the three tests are based on different principles, the correlation among them was excellent. The assays show differences in sensitivities with the ANB being the least sensitive of the three. The EIA equals the RIA in sensitivity, reproducibility, accuracy and labor and provides the advantage of safety and convenience in the use of non-radioactive materials. Therefore, the EIA has been selected as the most suitable assay for initial screening of the sera of patients receiving Roferon-A for the presence of antibodies to this interferon. EIA positive sera are then tested in the ANB to determine whether or not neutralizing activities are present.</div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"15 3","pages":"Pages 231-244"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(87)90026-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14430213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 56

The quantitation of human growth hormone by a radioreceptor assay using an established human cell line 利用已建立的人类细胞系，用放射受体测定法测定人类生长激素

Journal of biological standardization Pub Date : 1987-01-01 DOI: 10.1016/0092-1157(87)90023-0

Thore Nederman , Lars Sjödin

{"title":"The quantitation of human growth hormone by a radioreceptor assay using an established human cell line","authors":"Thore Nederman , Lars Sjödin","doi":"10.1016/0092-1157(87)90023-0","DOIUrl":"10.1016/0092-1157(87)90023-0","url":null,"abstract":"<div>Membrane receptors on cultured human lymphocytes (IM-9) have been shown to bind human growth hormone (hGH) in a specific manner. The aim of the present study was to develop an in vitro assay of hGH based on this binding. The assay should fulfil established pharmacopoeial requirements for quantitation of hormones. The binding of [125I]hGH was studied as a function of time, temperature, cell density, tracer concentration and the concentration of unlabelled hGH and other related hormones. Also, the dissociation of bound hGH and the chemical stability of hGH in the incubation medium were studied. From these studies, the conditions for an appropriate radioreceptor assay were determined. Briefly, 1·5–3·0 × 107 cells ml−1 were incubated with 5–20 × 10−12 m [125I]hGH and three different concentrations of unlabelled hGH chosen from the linear part of the [125I]hGH displacement curve. The results were analyzed according to general pharmacopoeial principles. The mean values for growth hormone activity tested by radioreceptor assay were within the fiducial limits (P = 0·05) of the corresponding activity determined by the hypophysectomized rat body-weight gain assay. The in vitro assay was found to be more precise and less resource demanding than the in vivo bioassay of hGH. It is concluded that the in vitro bioassay described here is well suited as a screening method for potency determination of hGH preparations.</div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"15 3","pages":"Pages 199-211"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(87)90023-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14732073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

The detoxification of Bordetella pertussis with glutaraldehyde 戊二醛对百日咳杆菌的解毒作用

Journal of biological standardization Pub Date : 1987-01-01 DOI: 10.1016/0092-1157(87)90013-8

T. Iida , Y. Horiuchi

引用次数: 8

A modification of the natural killer cell assay by substitution of 111indium for 51Chromium 用111铟代替51铬对自然杀伤细胞测定法的改进

Journal of biological standardization Pub Date : 1987-01-01 DOI: 10.1016/S0092-1157(87)80011-2

Andrew D. Blann

{"title":"A modification of the natural killer cell assay by substitution of 111indium for 51Chromium","authors":"Andrew D. Blann","doi":"10.1016/S0092-1157(87)80011-2","DOIUrl":"10.1016/S0092-1157(87)80011-2","url":null,"abstract":"<div>Natural killer (NK) cells are a heterogenous subgroup of lymphocytes possessing the ability to lyse spontaneously various normal, virally infected and transformed cell lines without prior sensitization. Although their lytic activity can be augmented by interferon and interleukin-2, and their function can be inhibited by prostaglandins and corticosteroids, their precise physiological or immunological role has not yet been determined. Roles in anti-tumour surveillance, resistance to viral infection and haemopoietic regulation have been suggested. NK activity has been shown to be defective in a number of diverse conditions such as the Chediak-Higashi syndrome, systemic lupus erythematosus, sarcoidosis and pregnancy. Most current estimations of NK activity rely on a modification of Brunner's cell mediated cytotoxicity assay in which cells bearing NK activity are co-cultured with chromium-labelled K562, a cell line derived from a myeloid leukaemia. However, the work of this and other groups have demonstrated the advantages of indium in isotope-release assays. Hence the experiments described in this report were undertaken to evaluate the use of indium in the NK/K562 assay.Cell line K562 was maintained in permanent cell culture at 37°C in a humidified atmosphere of 5% CO2, 95% air in RPMI 1640 cell-culture medium supplemented with 10% foetal calf serum (FCS), 100 IU ml-1 of penicillin, 100 μg ml-1 of streptomycin and 2 mM of L-glutamine. All cell culture plastics and medium were obtained from Flow or Gibco.</div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"15 4","pages":"Pages 379-383"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0092-1157(87)80011-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14800230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison of an enzyme-linked immunosorbent assay and counter current electrophoresis for the detection of bovine serum albumin in virus vaccines 酶联免疫吸附法和逆流电泳法检测病毒疫苗中牛血清白蛋白的比较

Journal of biological standardization Pub Date : 1987-01-01 DOI: 10.1016/0092-1157(87)90027-8

Anja R. ter Avest , Gijsbert van Steenis , Albert D.M.E. Osterhaus

引用次数: 8

The use of reverse indirect haemagglutination test for the in-process quality control of Japanese encephalitis vaccine 反向间接血凝试验在乙型脑炎疫苗生产过程质量控制中的应用

Journal of biological standardization Pub Date : 1987-01-01 DOI: 10.1016/0092-1157(87)90032-1

R.K. Gupta , V.K. Mehta , V.K. Gupta , C.N. Misra , S.N. Saxena

引用次数: 6

Human rabies immunoglobulin assayed by the rapid fluorescent focus inhibition test suppresses active rabies immunization 快速荧光聚焦抑制试验测定人狂犬病免疫球蛋白抑制狂犬病主动免疫

Journal of biological standardization Pub Date : 1987-01-01 DOI: 10.1016/0092-1157(87)90040-0

R. Glück , A. Wegmann , H. Keller , J.M. Hoskins , R. Germanier

引用次数: 10

The immunization of children with combined diphtheria and tetanus vaccine in Sweden 瑞典儿童白喉和破伤风联合疫苗的免疫接种

Journal of biological standardization Pub Date : 1987-01-01 DOI: 10.1016/0092-1157(87)90033-3

Kerstin Larsen , Karin Ullberg-Olsson , Birgitta Wickbom , Bengt Hederstedt

{"title":"The immunization of children with combined diphtheria and tetanus vaccine in Sweden","authors":"Kerstin Larsen , Karin Ullberg-Olsson , Birgitta Wickbom , Bengt Hederstedt","doi":"10.1016/0092-1157(87)90033-3","DOIUrl":"10.1016/0092-1157(87)90033-3","url":null,"abstract":"<div>Two groups derived from 97 children three-four months of age were vaccinated with diphtheria and tetanus vaccines containing either a routinely prepared diphtheria toxoid or a more purified preparation. Two injections were given with an interval of one month and a third injection was given one year after the first.Prior to the third injection no child was without protection against diphtheria, i.e. had an antitoxin titre <0·01 IU ml−1. After the third injection 95 and 94% of the children vaccinated with the routinely and more purified diphtheria toxoids, respectively, had diphtheria antitoxin titres >1 IU ml−1 (estimated to provide protection for at least ten years).Systemic reactions such as fever and malaise occurred in five children. Local reactions >10 cm were observed in three children and reactions >5 but ⩽10 cm were seen in 14% of the children.The routinely prepared combined diphtheria and tetanus vaccine, DT, produced very good immunity against diphtheria with moderate side effects. The use of a more purified diphtheria toxoid in the combined vaccine produced the same immunity and side effects.</div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"15 2","pages":"Pages 103-107"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(87)90033-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14719176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

The immunization of adults against diphtheria in Sweden 瑞典成人白喉免疫接种

Journal of biological standardization Pub Date : 1987-01-01 DOI: 10.1016/0092-1157(87)90034-5

Kerstin Larsen , Karin Ullberg-Olsson , Erik Ekwall , Bengt Hederstedt

{"title":"The immunization of adults against diphtheria in Sweden","authors":"Kerstin Larsen , Karin Ullberg-Olsson , Erik Ekwall , Bengt Hederstedt","doi":"10.1016/0092-1157(87)90034-5","DOIUrl":"10.1016/0092-1157(87)90034-5","url":null,"abstract":"<div>Two hundred and three women who disclaimed vaccination against diphtheria were divided into four groups and injected with either 2·0 or 6·25 Lf of a routine diphtheria toxoid or of a more purified preparation. One hundred and twenty-six of these women who did not show a secondary antibody response were given a second and a third injection one month and one year, respectively, after the first injection.Prebooster (third injection) antitoxin titres of ⩾0·01 IU ml−1 (the minimum level tor protection) were found in 22 and 37% of those who received 2·0 and 6·25 Lf, respectively. Postbooster titres of ⩾1·0 IU ml−1 (calculated to give a protection of at least ten years of duration) were found in 23 and 58% of those who received 2·0 and 6·25 Lf, respectively.The rate of untoward reactions was low. Fever of short duration occurred in five women. Four out of the five women received 6·25 Lf of the more purified diphtheria toxoid and one 2 Lf of the routine toxoid. Local reactions >10 cm were observed in three women. All received the higher dose, 6·25 Lf of diphtheria toxoid. Local reactions >5 but ⩽10 cm occurred in up to 13% (6·25 Lf of diphtheria toxoid). No significant difference between the groups of women vaccinated with routine or more purified toxoid was found.It was concluded that the diphtheria toxoids in the two doses of 2 Lf and 6·25 Lf did not induce a satisfactory immune response. To induce adequate protection the dose of diphtheria vaccine needs to be the same for adults and children, i.e. 12·5 Lf.</div>","PeriodicalId":75991,"journal":{"name":"Journal of biological standardization","volume":"15 2","pages":"Pages 109-116"},"PeriodicalIF":0.0,"publicationDate":"1987-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0092-1157(87)90034-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14719177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9