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引用次数: 2
摘要
利用纯化的肠病毒吸附在硝化纤维素上，建立了一种简单的间接酶免疫分析法，用于筛选肠病毒单克隆抗体。该方法对病毒蛋白的灵敏度为10 ng ~ 1 μg，比传统的微孔板上的EIA灵敏度高10 ~ 50倍。这种简单、灵敏、特异的检测方法被证明是检测单克隆抗体的一种有用的实用工具，这种单克隆抗体在传统的EIA筛选程序中找不到。
The use of a nitrocellulose-enzyme immunoassay for the rapid screening of monoclonal antibodies to human enteroviruses
A simple, indirect enzyme immunoassay using purified enterovirus adsorbed on to nitrocellulose has been developed for screening monoclonal antibodies to enteroviruses. The sensitivity of the assay ranged from 10 ng to 1 μg of viral protein and was 10- to 50-fold more sensitive than conventional EIA on microplates. This simple, sensitive and specific assay proved to be a useful and practical tool for detecting monoclonal antibodies which would not be found in a conventional EIA screening procedure.
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