The toxin binding inhibition test as a reliable in vitro alternative to the toxin neutralization test in mice for the estimation of tetanus antitoxin in human sera

Journal of biological standardization Pub Date : 1988-01-01 DOI:10.1016/0092-1157(88)90017-0

C.F.M. Hendriksen , J.W.v.d. Gun , J. Nagel , J.G. Kreeftenberg

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引用次数: 84

Abstract

A method for the screening of human sera for tetanus antibodies has been developed and evaluated. The toxin binding inhibition test (ToBI-test) is based on inhibition of the binding of tetanus toxin to an antitoxin-coated immunoassay microtitre plate by tetanus antibodies. Serum samples from 191 healthy adults with different vaccination histories have been titrated for tetanus antibodies by the toxin neutralization (TN) test in mice, by toxoid-ELISA and by the ToBI-test. In every respect, the ToBI-test proved to be the best in vitro alternative to the TN-test in mice. Comparisons showed a higher degree of correlation between the ToBI-test and the TN-test than between the toxoid-ELISA and the TN-test. Furthermore, no overestimation of antibody content was seen in titrating low titre sera by the ToBI-test. In contrast, several false positive results were seen when using the toxoid-ELISA. It is concluded that the ToBI-test is a reliable and precise alternative to the TN-test and can be performed under simple laboratory conditions in a short time.

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毒素结合抑制试验是测定人血清中破伤风抗毒素的可靠的体外替代毒素中和试验方法

一种筛选人血清破伤风抗体的方法已被开发和评价。毒素结合抑制试验(tobi试验)是基于破伤风抗体抑制破伤风毒素与抗毒素包被免疫测定微滴板的结合。采用小鼠毒素中和(TN)试验、类毒素elisa和tobi试验对191例不同接种史的健康成人血清样本进行破伤风抗体滴定。在各方面，tobi试验在小鼠体内被证明是最好的体外替代tn试验。比较显示tobi试验与tn -试验的相关性高于类毒素elisa与tn -试验的相关性。此外，在用tobi试验滴定低滴度血清时，没有发现抗体含量的高估。相反，当使用类毒素elisa时，出现了一些假阳性结果。结果表明，tobi试验是tn试验的可靠、精确的替代方法，可在简单的实验室条件下在短时间内完成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of biological standardization

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